標(biāo)題: Titlebook: Hedgehog Signaling; Methods and Protocol Xiaochun Li Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to [打印本頁(yè)] 作者: APL 時(shí)間: 2025-3-21 19:59
書(shū)目名稱Hedgehog Signaling影響因子(影響力)
作者: hauteur 時(shí)間: 2025-3-21 21:21 作者: 泛濫 時(shí)間: 2025-3-22 04:14 作者: 宏偉 時(shí)間: 2025-3-22 06:26
Sandii Constable,Kevin White,Bandarigoda Nipunika Somatilaka,Saikat Mukhopadhyayinds of principles and relations which hold necessarily but, on occasions, are defeasible if a competing value demands preference .. The focus here is to show that our argument from defeasibility and justified violation can help bridging the gap between ethics and its application. This also reveals the crucial trait of rational morality.作者: Orchiectomy 時(shí)間: 2025-3-22 12:28
Yuhong Han,Jin Jiangstructing evolution process models are presented. Furthermore, a procedure for modelling software evolution processes at the global level is developed. Finally, the relationship between descriptions and models of software evolution processes is also discussed.作者: Kinetic 時(shí)間: 2025-3-22 14:33 作者: 吸引力 時(shí)間: 2025-3-22 19:52
Characterization of Smoothened Phosphorylation and Activation,ng in vitro kinase assay, phospho-specific antibodies, luciferase reporter assay, cell surface accumulation, and ciliary localization assays. These assays provide powerful tools to study Smo phosphorylation and activation, leading to mechanistic insight into Smo regulation.作者: GAVEL 時(shí)間: 2025-3-23 00:48
Generation and Analysis of Mosaic Spinal Cord Organoids Derived from Mouse Embryonic Stem Cells,n approach using genetically modified murine stem cell lines to individually address the contribution of these cells in the establishment and response to a morphogenetic gradient in mosaic spinal cord organoids.作者: 多產(chǎn)子 時(shí)間: 2025-3-23 03:07
Analysis of Protein Cholesterylation by Biorthogonal Labeling,sent a protocol of a chemical biology method to analyze the Smoothened protein cholesterylation using an azido-conjugated cholesterol analog combined with click chemistry. This method can be applied to analyze Hedgehog cholesterylation, and be extended to identify novel cholesterylated proteins with minor changes.作者: FLAT 時(shí)間: 2025-3-23 08:33
An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched,living cells. When applied to the hedgehog signaling system, it enables direct quantitative measurement of the cholesterol transporter activity of Patched1 and the effect of the hedgehog ligand on this activity.作者: 協(xié)議 時(shí)間: 2025-3-23 12:19
,Expression, Purification, and Structure Determination of Human PTCH1–HH-N Complexes,ng the therapeutic development of cancers. Here, we describe the expression and purification of a nearly full-length functional PTCH1 variant, PTCH1*. With purified PTCH1* protein, two forms of PTCH1*–Sonic Hedgehog (SHH) complexes were assembled, and their structures subsequently determined by cryo-electron microscope (cryo-EM).作者: 事情 時(shí)間: 2025-3-23 13:57
Rapid, Direct SMOOTHENED Activity Assays in Live Cells Using cAMP-Based Conformational Sensors,ing changes in SMO conformation within minutes of PTCH1 inactivation by Hh ligands. As a result, it expands the range of experimental perturbations that can be used to dissect PTCH1-SMO communication, enabling a deeper mechanistic understanding of a longstanding mystery in Hh signal transduction.作者: Cryptic 時(shí)間: 2025-3-23 22:05 作者: cortex 時(shí)間: 2025-3-24 00:37
Light-Induced Differentiation of Forebrain Organoids by NVOC-SAG,y induce ventral differentiation of human iPS cell-derived forebrain organoids in a light-dependent manner. This article describes preparation of NVOC-SAG, culture of iPS cell-derived forebrain organoids, light irradiation, and downstream analyses.作者: d-limonene 時(shí)間: 2025-3-24 04:06 作者: 胡言亂語(yǔ) 時(shí)間: 2025-3-24 07:08
Analysis of Dispatched Protein Processing and Sonic Hedgehog Ligand Release,pter, we present a ligand release/retention protocol that allows for the analysis of DISP cleavage, DISP-mediated release of SHH ligand from producing cells, and secretion-dependent signal induction in target cells.作者: 完全 時(shí)間: 2025-3-24 13:01
Cell-Based Assays for Smoothened Ubiquitination and Sumoylation,h, which counteracts Smo ubiquitination by recruiting the dub USP8. We describe cell-base assays for Smo ubiquitination and its regulation by Hh and the E3 ligases in .. We also describe assays for Smo sumoylation in both . and mammalian cultured cells.作者: gentle 時(shí)間: 2025-3-24 17:10 作者: Palpate 時(shí)間: 2025-3-24 21:30 作者: 打火石 時(shí)間: 2025-3-25 02:25 作者: 火車車輪 時(shí)間: 2025-3-25 05:08 作者: 魔鬼在游行 時(shí)間: 2025-3-25 08:46 作者: Terminal 時(shí)間: 2025-3-25 13:12
An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched,. This ratiometric fluorescence technique allows real-time monitoring of the cholesterol transporter activity of plasma membrane-resident proteins in living cells. When applied to the hedgehog signaling system, it enables direct quantitative measurement of the cholesterol transporter activity of Pat作者: Irksome 時(shí)間: 2025-3-25 16:18 作者: boisterous 時(shí)間: 2025-3-25 22:06 作者: Lucubrate 時(shí)間: 2025-3-26 00:56 作者: 殺蟲(chóng)劑 時(shí)間: 2025-3-26 05:44
The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured ugh primary cilia affect Hedgehog signaling, and many Hedgehog pathway components traffic through or accumulate in cilia. This protocol provides methods for immunofluorescence staining of cilia-accumulated Hh pathway components, such as Smoothened, in cultured NIH 3T3 cells.作者: Hearten 時(shí)間: 2025-3-26 11:21 作者: puzzle 時(shí)間: 2025-3-26 12:57
,Expression, Purification, and Structure Determination of Human PTCH1–HH-N Complexes,PTCH1 are implicated in many human cancers. Structural investigation revealed the mechanism of PTCH1-mediated HH signal regulation, further facilitating the therapeutic development of cancers. Here, we describe the expression and purification of a nearly full-length functional PTCH1 variant, PTCH1*.作者: 不足的東西 時(shí)間: 2025-3-26 20:35 作者: 走調(diào) 時(shí)間: 2025-3-26 22:44
Cell-Based Assays for Smoothened Ubiquitination and Sumoylation,d activity of the GPCR family protein Smoothened (Smo). Smo trafficking and subcellular accumulation are controlled by multiple posttranslational modifications (PTMs) including phosphorylation, ubiquitination, and sumoylation, which appears to be conserved from . to mammals. Smo ubiquitination is dy作者: BRIBE 時(shí)間: 2025-3-27 04:13 作者: MILK 時(shí)間: 2025-3-27 06:47
Studies of SMOOTHENED Activation in Cell-Free and Reconstituted Systems,of cell-free and reconstituted systems to study a key step in Hedgehog signal transduction: the activation of SMOOTHENED by membrane lipids. These methods can be adapted to study other steps in Hedgehog signal transduction, particularly those that occur at the membrane.作者: 嚴(yán)厲批評(píng) 時(shí)間: 2025-3-27 10:05
Rapid, Direct SMOOTHENED Activity Assays in Live Cells Using cAMP-Based Conformational Sensors,e a real-time cell-based SMO functional assay based on SMO activity-dependent changes in cellular cAMP concentrations. This assay is capable of detecting changes in SMO conformation within minutes of PTCH1 inactivation by Hh ligands. As a result, it expands the range of experimental perturbations th作者: armistice 時(shí)間: 2025-3-27 14:36
Light-Induced Differentiation of Forebrain Organoids by NVOC-SAG,its ligands finely controls the activity of the Hedgehog pathway during development. To mimic the active regulation of Hedgehog pathway, we have developed a light-inducible Hedgehog signaling activator 6-nitroveratryloxy-carbonyl Smoothened agonist (NVOC-SAG). Here we describe a method to selectivel作者: Recessive 時(shí)間: 2025-3-27 19:55 作者: oxidize 時(shí)間: 2025-3-28 01:09
Measuring Smoothened (SMO)-Mediated Activation of the Gi Protein,nscription factors. Previous studies have shown that SMO is a G-protein-coupled receptor (GPCR) of the Frizzled-class (class-F) that can activate G. family of heterotrimeric G proteins. Here, we describe [.S]-GTPγS assay using SMO cell membranes and purified G. protein to measure the level of G. pro作者: creditor 時(shí)間: 2025-3-28 04:56 作者: Ingenuity 時(shí)間: 2025-3-28 06:46 作者: Hypomania 時(shí)間: 2025-3-28 14:09
Carina J?gers,Henk Roelink. Accessory cells are known to collaborate with lymphocytes and enable them to carry out their essential functions. FDC present antigen (Ag) to B cells and facilitate B cell functions; they may be thought of as B cell-associated accessory cells. In contrast, other dendritic cells, including Langerha作者: Abrade 時(shí)間: 2025-3-28 17:49
Sebastian A. Andrei,Edward W. Tate,Thomas Lanyon-Hoggelligent agents that can cooperatively support production and logistics network decisions, as well as to compare their performance to other more traditional methods. A short description of supply chains is given, as well as a formal characterization of the problem space under investigation. We outli作者: Arresting 時(shí)間: 2025-3-28 19:33 作者: 博識(shí) 時(shí)間: 2025-3-29 01:59 作者: refine 時(shí)間: 2025-3-29 03:05
Tatsuo Miyamoto,Kosuke Hosoba,Silvia Natsuko Akutsu,Shinya Matsuuras the complexity of choosing which multi-agent control technologies are appropriate for a given problem domain or a given application. Without such knowledge, when faced with a new application domain, agent developers must rely on past experience and intuition to determine whether a multi-agent syst作者: 聰明 時(shí)間: 2025-3-29 09:50 作者: 得體 時(shí)間: 2025-3-29 13:42
Maia Kinnebrew,Kristen A. Johnson,Arun Radhakrishnan,Rajat Rohatgis. Here we question the need for the ethical point of view; we ask for justification of ethics?– justification of our being moral. This chapter treats this question as an improper question since it is a question about something that is normally presupposed by the question itself. One reason why ethi作者: hegemony 時(shí)間: 2025-3-29 19:14 作者: CHASM 時(shí)間: 2025-3-29 19:49
Elizabeth Cleverdon,Daniel P. Stewart,Stacey K. Ogdenefficient management of knowledge bases, with the aim of filling part of the technological gap between artificial intelligence and databases. The book first investigates in detail the design process, the architecture, and the working methods of knowledge based systems (KS) in order to point out the 作者: 祝賀 時(shí)間: 2025-3-30 00:25
Xiaofeng Qi,Philip Schmiege,Leticia Esparza,Xiaochun Liefficient management of knowledge bases, with the aim of filling part of the technological gap between artificial intelligence and databases. The book first investigates in detail the design process, the architecture, and the working methods of knowledge based systems (KS) in order to point out the 作者: Predigest 時(shí)間: 2025-3-30 05:58 作者: Mere僅僅 時(shí)間: 2025-3-30 08:51 作者: 胡言亂語(yǔ) 時(shí)間: 2025-3-30 16:06 作者: 沖擊力 時(shí)間: 2025-3-30 20:20
Isaac B. Nelson,Benjamin R. Myersctive and efficient management of knowledge bases, with the aim of filling part of the technological gap between artificial intelligence and databases. The book first investigates in detail the design process, the architecture, and the working methods of knowledge based systems (KS) in order to poin作者: IRK 時(shí)間: 2025-3-30 23:52
Madison F. Walker,Benjamin R. Myersctive and efficient management of knowledge bases, with the aim of filling part of the technological gap between artificial intelligence and databases. The book first investigates in detail the design process, the architecture, and the working methods of knowledge based systems (KS) in order to poin作者: 極端的正確性 時(shí)間: 2025-3-31 02:56
Ryuji Misawa,Yoshiho Ikeuchiefficient management of knowledge bases, with the aim of filling part of the technological gap between artificial intelligence and databases. The book first investigates in detail the design process, the architecture, and the working methods of knowledge based systems (KS) in order to point out the 作者: neutrophils 時(shí)間: 2025-3-31 05:41
Pawe? Kozielewicz,Gunnar Schulteefficient management of knowledge bases, with the aim of filling part of the technological gap between artificial intelligence and databases. The book first investigates in detail the design process, the architecture, and the working methods of knowledge based systems (KS) in order to point out the 作者: 令人苦惱 時(shí)間: 2025-3-31 09:33
Heng Liu,Cheng Zhanglassical waterfall model of the software life cycle. This case study aims to illustrate the proposed approach to modelling and describing classical software processes. The second case study is about a set of software processes which describe the evolution of a software system. This case study aims t作者: Incisor 時(shí)間: 2025-3-31 14:47
Yuhong Han,Jin Jianged by EPMM and EPDL is discussed. The framework is a combination of both hierarchical and an object-oriented frameworks. Firstly, a software evolution process forms a four-level framework: the global level, the process level, the activity level and the task level. Each level possesses specific struc作者: 不斷的變動(dòng) 時(shí)間: 2025-3-31 20:26
Imaging of the Ciliary Cholesterol Underlying the Sonic Hedgehog Signal Transduction,f ciliary cholesterol using the fluorescent small compound Filipin III and the green fluorescent protein tagged Domain 4 of the exotoxin Perfringolysin O derived from the anaerobic bacterium .. These cholesterol probes should be powerful tools for understanding the physiological and pathological rol作者: 出生 時(shí)間: 2025-4-1 00:50 作者: 狂熱文化 時(shí)間: 2025-4-1 02:06 作者: 侵蝕 時(shí)間: 2025-4-1 06:42 作者: 證明無(wú)罪 時(shí)間: 2025-4-1 11:12
Ao Hu,Meng Zhou,Bao-Liang Songal agents and groups of agents in accomplishing an inter-dependent set of goals in a shared simulation under a diverse set of environmental conditions. The findings are explained in text and a rich data visualization. The analysis details the effects of the individual interaction styles, formation o作者: 綠州 時(shí)間: 2025-4-1 14:45 作者: 直覺(jué)沒(méi)有 時(shí)間: 2025-4-1 19:26
Tatsuo Miyamoto,Kosuke Hosoba,Silvia Natsuko Akutsu,Shinya Matsuuraats involved with each decision. ..An Application Science For Multi-Agent Systems. is an excellent reference for anyone involved in developing multi-agent systems. .978-1-4757-7923-3978-1-4020-7868-2Series ISSN 1568-2617 作者: 信徒 時(shí)間: 2025-4-2 01:19
