標(biāo)題: Titlebook: Handbook of ELISPOT; Methods and Protocol Alexander E. Kalyuzhny Book 20051st edition Humana Press 2005 Antigen.Termination.antibody.biolog [打印本頁] 作者: magnify 時(shí)間: 2025-3-21 16:41
書目名稱Handbook of ELISPOT影響因子(影響力)
書目名稱Handbook of ELISPOT影響因子(影響力)學(xué)科排名
書目名稱Handbook of ELISPOT網(wǎng)絡(luò)公開度
書目名稱Handbook of ELISPOT網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Handbook of ELISPOT被引頻次
書目名稱Handbook of ELISPOT被引頻次學(xué)科排名
書目名稱Handbook of ELISPOT年度引用
書目名稱Handbook of ELISPOT年度引用學(xué)科排名
書目名稱Handbook of ELISPOT讀者反饋
書目名稱Handbook of ELISPOT讀者反饋學(xué)科排名
作者: Cubicle 時(shí)間: 2025-3-21 22:44
Alvin H. Strelnick,Margaret Gilpinexperienced scientists as well as newcomers to the field an overview over the major standardization issues for each step of the protocol. Guidelines are given on how to validate the ELISPOT performance.作者: 不可接觸 時(shí)間: 2025-3-22 04:13 作者: 除草劑 時(shí)間: 2025-3-22 05:45 作者: GUMP 時(shí)間: 2025-3-22 10:00 作者: 聽覺 時(shí)間: 2025-3-22 13:50 作者: 刺激 時(shí)間: 2025-3-22 19:11
Mohd Farid Ahmad,Muhammad Syahmi Hishamuddinymphocytes with antigens. In rhesus macaques immunized with an HIV envelope peptide cocktail vaccine, the DC-ELISPOT protocol enabled more accurate enumeration of the cellular immune responses, as antigen-specific inteferon-γ-producing cells, with up to 18-fold increase in detection sensitivity comp作者: LASH 時(shí)間: 2025-3-23 00:11 作者: 熱心助人 時(shí)間: 2025-3-23 01:54
Urban Identity and the Atlantic Worldoteins of myelin: myelin basic protein and proteolipid protein. Our goal was to use the enzyme-linked immunospot assay to perform comprehensive epitope mapping in relapsing-remitting MS patients in a longitudinal study to help define the role of myelin responses in disease progression.作者: 輕率的你 時(shí)間: 2025-3-23 07:55
Standardization and Validation Issues of the ELISPOT Assayexperienced scientists as well as newcomers to the field an overview over the major standardization issues for each step of the protocol. Guidelines are given on how to validate the ELISPOT performance.作者: 愛社交 時(shí)間: 2025-3-23 13:08 作者: 燈絲 時(shí)間: 2025-3-23 14:14 作者: 出汗 時(shí)間: 2025-3-23 19:38
Image Analysis and Data Management of ELISPOT Assay ResultsPractice and Code for Federal Regulations Part 11 guidelines. In addition, ELISPOT assays in clinical and basic science settings frequently require high degrees of throughput, thus further raising the need for advanced data management and statistical analysis. The ImmunoSpot software port-folio has 作者: 沒血色 時(shí)間: 2025-3-23 22:56
High Resolution as a Key Feature to Perform Accurate ELISPOT Measurements Using Zeiss KS ELISPOT Reasmooth borderline. Resolution is a key feature for a precise detection of ELISPOT. In standard applications shape and edge steepness are essential parameters in addition to size and color for an accurate spot recognition. These parameters need a minimum spot diameter of 6 pixels. Collecting one sing作者: right-atrium 時(shí)間: 2025-3-24 06:02 作者: agnostic 時(shí)間: 2025-3-24 08:46
Feline Cytokine ELISPOTmmunodeficiency virus. Broader applications of the ELISPOT should further our understanding of feline diseases and be useful in the rational development of more efficacious vaccines and therapeutic modalities for the enhancement of feline health. This chapter discusses important parameters of ELISPO作者: 暗諷 時(shí)間: 2025-3-24 13:30
Epitope Mapping in Multiple Sclerosis Using the ELISPOT Assayoteins of myelin: myelin basic protein and proteolipid protein. Our goal was to use the enzyme-linked immunospot assay to perform comprehensive epitope mapping in relapsing-remitting MS patients in a longitudinal study to help define the role of myelin responses in disease progression.作者: preeclampsia 時(shí)間: 2025-3-24 18:17
https://doi.org/10.1385/1592599036Antigen; Termination; antibody; biology; cell; chemistry; cytokine; cytokines; development; immune response; i作者: microscopic 時(shí)間: 2025-3-24 22:13 作者: EXALT 時(shí)間: 2025-3-25 01:10
Alexander E. KalyuzhnyIncludes supplementary material: 作者: Immunoglobulin 時(shí)間: 2025-3-25 04:43 作者: 下垂 時(shí)間: 2025-3-25 07:30
City Structure and Spatial Patterns,f-the-art technique that requires accuracy, thorough selection of antibodies and detection reagents, and an understanding of the principles of data analysis. This review covers various technical aspects of the ELISPOT assay, including immunochemical principles of the assay, selection of reagents and plates, and troubleshooting recommendations.作者: 伙伴 時(shí)間: 2025-3-25 14:13
Peter Huggenberger,Jannis Eptingtion because antigen specific T-cells can occur at a low frequency .. This method has been successfully used to analyze . immune responses in humans in addition to murine and nonhuman primate models of tuberculosis.作者: uncertain 時(shí)間: 2025-3-25 17:15
Chemistry and Biology of the ELISPOT Assayf-the-art technique that requires accuracy, thorough selection of antibodies and detection reagents, and an understanding of the principles of data analysis. This review covers various technical aspects of the ELISPOT assay, including immunochemical principles of the assay, selection of reagents and plates, and troubleshooting recommendations.作者: diabetes 時(shí)間: 2025-3-25 23:54
Measuring T-Cell Function in Animal Models of Tuberculosis by ELISPOTtion because antigen specific T-cells can occur at a low frequency .. This method has been successfully used to analyze . immune responses in humans in addition to murine and nonhuman primate models of tuberculosis.作者: collagen 時(shí)間: 2025-3-26 01:06 作者: 無情 時(shí)間: 2025-3-26 06:30 作者: 平息 時(shí)間: 2025-3-26 11:00
Sandeep Narula,Narma Bhattacharyaalso outlined. These methods can also be broadly adapted to measure activated T-cells to other viral pathogens and/or pathogen-derived peptides. Therefore, the ELISPOT assay can be used for the design, development, and evaluation of new vaccines.作者: 證明無罪 時(shí)間: 2025-3-26 14:39
Pockets of Urban Informality in Lebanonnner ear-specific autoimmune targets in ASNHL, and our ELISPOT approach may be particularly useful in supporting the diagnosis of this disease entity. In the current chapter we detail how to use the ELISPOT assay for measuring frequencies of IFN-γ-producing T-cells in patients with ASNHL.作者: evince 時(shí)間: 2025-3-26 20:05
Membranes and Membrane Plates Used in ELISPOTybridization and protein chemistry applications. In this chapter, an overview of the development and biotechnology applications of nitrocellulose and polyvinylidene fluoride membrane is provided and characteristics and attributes of each of the membranes that are relevant to ELISPOT are summarized.作者: Meditative 時(shí)間: 2025-3-26 22:18 作者: ALIEN 時(shí)間: 2025-3-27 03:57
Detection of Measles Virus-Specific Interferon-γ-Secreting T-Cells by ELISPOTalso outlined. These methods can also be broadly adapted to measure activated T-cells to other viral pathogens and/or pathogen-derived peptides. Therefore, the ELISPOT assay can be used for the design, development, and evaluation of new vaccines.作者: 未成熟 時(shí)間: 2025-3-27 05:55 作者: 吹牛大王 時(shí)間: 2025-3-27 10:31 作者: 教唆 時(shí)間: 2025-3-27 14:42 作者: tangle 時(shí)間: 2025-3-27 19:48 作者: 全面 時(shí)間: 2025-3-27 22:23 作者: 喃喃訴苦 時(shí)間: 2025-3-28 04:35
ELISPOT Assaystances that lead to the assay’s development within the Perth group. Included are the early studies in 1981 through early 1982 that were conducted to bring the assay to fruition—both setbacks and solutions, and finally some generally unknown but amusing insights into the naming of the ELISPOT assay by the Gothenburg group.作者: 引導(dǎo) 時(shí)間: 2025-3-28 06:25 作者: 政府 時(shí)間: 2025-3-28 11:03 作者: 薄荷醇 時(shí)間: 2025-3-28 15:11
ELISPOT AssayWestern Australia, and the second, soon thereafter, from a group in Gothenburg, Sweden. Described here is my recollection of the background and circumstances that lead to the assay’s development within the Perth group. Included are the early studies in 1981 through early 1982 that were conducted to 作者: Horizon 時(shí)間: 2025-3-28 20:39 作者: 懶洋洋 時(shí)間: 2025-3-28 23:22
Membranes and Membrane Plates Used in ELISPOT The membranes in these plates are made from either nitrocellulose or polyvinylidene fluoride. These membranes are well suited for ELISPOT because they have high antibody binding capacities and because their white color provides an excellent backdrop for ELISPOT enumeration. These two membranes and,作者: 流行 時(shí)間: 2025-3-29 04:55
Standardization and Validation Issues of the ELISPOT Assayresponses in humans and a variety of other species. With the ELISPOT assay, immune cell frequencies can be measured at the single cell level without elaborate expansion or manipulation of cell populations. Its usefulness has led to its application in vaccine design and development and, most importan作者: SAGE 時(shí)間: 2025-3-29 11:15 作者: Yourself 時(shí)間: 2025-3-29 13:27 作者: Patrimony 時(shí)間: 2025-3-29 15:43
Image Analysis and Data Management of ELISPOT Assay Resultsof antigen-specific T-cells and also on the secretion rate of the individual cells is captured in spots generated using this technique. Although the overall assessment of ELISPOT results can be conducted visually, this is inevitably subjective, inaccurate, and cumbersome. In contrast, objective, and作者: 避開 時(shí)間: 2025-3-29 21:11
High Resolution as a Key Feature to Perform Accurate ELISPOT Measurements Using Zeiss KS ELISPOT Reaod has been improved, and ELISPOT is used for the determination of the production of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, or various interleukins (IL)-4, IL-5. ELISPOT measurements are performed in 96-well plates with nitrocellulose membranes either visually or by means of image analys作者: STENT 時(shí)間: 2025-3-30 01:00 作者: FADE 時(shí)間: 2025-3-30 07:00 作者: 誘導(dǎo) 時(shí)間: 2025-3-30 11:42 作者: 包庇 時(shí)間: 2025-3-30 15:52
Interferon-γ ELISPOT Assay for the Quantitative Measurement of Antigen-Specific Murine CD8+ T-Cellses. The enzyme-linked immunospot (ELISPOT) is a sensitive technique for the detection of cytokine-producing cells at the single cell level. This assay is rapid and reproducible and permits the direct enumeration of low-frequency antigen-specific T-cells. This protocol describes in detail an interfer作者: Ossification 時(shí)間: 2025-3-30 18:13 作者: uncertain 時(shí)間: 2025-3-30 22:14
Epitope Mapping in Multiple Sclerosis Using the ELISPOT Assayutoaggressive response causes the breakdown of the myelin sheaths that protect nerve axons, leading to impaired nerve conduction and subsequent neurodegeneration that are characteristic of MS. Many studies have attempted to determine the exact target within the brain. However, there appear to be mul作者: 道學(xué)氣 時(shí)間: 2025-3-31 02:49 作者: Intellectual 時(shí)間: 2025-3-31 07:55
ELISPOT Determination of Interferon-γ T-Cell Frequencies in Patients With Autoimmune Sensorineural H is unclear, it is widely believed that antibody and/or T-cell responses directed against inner ear-specific proteins may mediate ASNHL. Using the enzyme-linked immunospot (ELISPOT) assay, we have recently found that many patients with ASNHL have increased frequencies of peripheral blood T-cells cap作者: 饒舌的人 時(shí)間: 2025-3-31 10:39 作者: 矛盾 時(shí)間: 2025-3-31 15:34 作者: covert 時(shí)間: 2025-3-31 20:42
https://doi.org/10.1007/978-981-13-0308-1 The membranes in these plates are made from either nitrocellulose or polyvinylidene fluoride. These membranes are well suited for ELISPOT because they have high antibody binding capacities and because their white color provides an excellent backdrop for ELISPOT enumeration. These two membranes and,
