標(biāo)題: Titlebook: Handbook of ELISPOT ; Methods and Protocol Alexander E. Kalyuzhny Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), [打印本頁(yè)] 作者: 緩和緊張狀況 時(shí)間: 2025-3-21 16:05
書(shū)目名稱Handbook of ELISPOT 影響因子(影響力)
書(shū)目名稱Handbook of ELISPOT 影響因子(影響力)學(xué)科排名
書(shū)目名稱Handbook of ELISPOT 網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱Handbook of ELISPOT 網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱Handbook of ELISPOT 被引頻次
書(shū)目名稱Handbook of ELISPOT 被引頻次學(xué)科排名
書(shū)目名稱Handbook of ELISPOT 年度引用
書(shū)目名稱Handbook of ELISPOT 年度引用學(xué)科排名
書(shū)目名稱Handbook of ELISPOT 讀者反饋
書(shū)目名稱Handbook of ELISPOT 讀者反饋學(xué)科排名
作者: iodides 時(shí)間: 2025-3-21 23:12
Joost Hauer,Harry Timmermans,Neil Wrigleyas been proposed that the detection and measurement of memory B cells capable of secreting antibodies against donor HLA antigens could be carried out using B-cell ImmunoSpot, to better inform the degree of immune sensitization of transplant patients prior to as well as after transplantation. Such an assay is described here.作者: ironic 時(shí)間: 2025-3-22 01:36 作者: entreat 時(shí)間: 2025-3-22 06:23
Using PBMCs in a Multiplex FluoroSpot Assay for Detection of Innate Immune Response-Modulating Impuse of a triple-color IL-1β/IL-6/TNF-α FluoroSpot assay as a sensitive tool for quantification of the frequencies of IIRMI-activated monocytes as well as determination of the relative amount of pyrogenic cytokine(s) produced by each activated cell.作者: 施舍 時(shí)間: 2025-3-22 11:19
1064-3745 ation advice from the experts.This fourth edition volume expands on the previous editions with discussions on the latest protocols that can be applied in different laboratory landscapes ranging from purely research to clinical diagnostic environments. This volume particularly focuses on ELISpot vali作者: JAUNT 時(shí)間: 2025-3-22 13:51
https://doi.org/10.1007/978-981-13-1168-0se of a triple-color IL-1β/IL-6/TNF-α FluoroSpot assay as a sensitive tool for quantification of the frequencies of IIRMI-activated monocytes as well as determination of the relative amount of pyrogenic cytokine(s) produced by each activated cell.作者: sparse 時(shí)間: 2025-3-22 20:50 作者: Commission 時(shí)間: 2025-3-22 21:40 作者: Culpable 時(shí)間: 2025-3-23 04:01
Urban Biodiversity and Built EnvironmentLISpot protocol has been left to diverse opinions about how to interpret and tackle typical validation parameters. This chapter addresses important considerations for ELISpot validation, including the interpretations of validation parameters for a meaningful description of assay performance.作者: RECUR 時(shí)間: 2025-3-23 06:52 作者: Estrogen 時(shí)間: 2025-3-23 12:02
Important Considerations for ELISpot Validation,LISpot protocol has been left to diverse opinions about how to interpret and tackle typical validation parameters. This chapter addresses important considerations for ELISpot validation, including the interpretations of validation parameters for a meaningful description of assay performance.作者: 焦慮 時(shí)間: 2025-3-23 14:38 作者: landmark 時(shí)間: 2025-3-23 18:20 作者: 束縛 時(shí)間: 2025-3-23 23:58
Handbook of ELISPOT 978-1-0716-3690-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 密切關(guān)系 時(shí)間: 2025-3-24 04:21
Alexander E. KalyuzhnyIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: ILEUM 時(shí)間: 2025-3-24 08:42 作者: pessimism 時(shí)間: 2025-3-24 11:42 作者: 古董 時(shí)間: 2025-3-24 16:50 作者: 財(cái)政 時(shí)間: 2025-3-24 20:27
https://doi.org/10.1007/978-4-431-56856-8eting cells. In addition, this method can measure biological activity and immunological secretions from immune cells. In general, membrane-bound antigen allows binding of antibody secreted by B cells, or a membrane-bound analyte-specific antibody binds to the specific analyte (e.g., cytokines) elici作者: beta-carotene 時(shí)間: 2025-3-25 01:50
Zhiming Cheng,Mark Wang,Junhua ChenISPOT), which depend on antigen-specific stimulation, increasingly use peptides to represent the antigen of interest. Besides single peptides, mixtures of peptides (peptide pools) are very frequently applied. Such peptide pools may, for example, represent entire proteins (with overlapping peptides c作者: 固執(zhí)點(diǎn)好 時(shí)間: 2025-3-25 03:46 作者: 格言 時(shí)間: 2025-3-25 09:49
Nubia Beray Armond,Jo?o Lima Sant’Anna Netoibodies that B cells secrete, inherently result in a broad range of spot sizes and intensities. The diversity of secretory footprint morphologies reflects the polyclonal nature of the antigen-specific B cell repertoire, with individual antibody-secreting B cells in the test sample differing in their作者: 我就不公正 時(shí)間: 2025-3-25 12:10
ion has its own advantages and challenges. ELISpot is more sensitive compared to ELISA and appears to be more consistent in detecting IL-10 production than flow cytometry. ELISpot can be used for detecting the secretion of multiple cytokines but not from the same cells simultaneously, whereas flow c作者: granite 時(shí)間: 2025-3-25 19:25
Democracy in big cities: the case of Germanyd cell material, such as peripheral blood mononuclear cells (PBMC). Establishing their frequency within all PBMC permits to assess the magnitude of antigen-specific T-cell immunity; the simultaneous measurement of their cytokine signatures reveals these T-cells’ lineage and effector functions, that 作者: 夾克怕包裹 時(shí)間: 2025-3-25 22:58 作者: 使閉塞 時(shí)間: 2025-3-26 00:58
Urban Design and Representation and 2 (FCoV2). Cats naturally infected with FCoV1 have been shown to possess serum reactivities with FCoV1 and SCoV2 RBDs but not with FCoV2 RBD. In the current study, COVID-19-vaccinated humans and FCoV1-infected laboratory cats were evaluated for interferon-gamma (IFNγ) and interleukin-2 (IL-2 EL作者: overweight 時(shí)間: 2025-3-26 04:33 作者: 表示問(wèn) 時(shí)間: 2025-3-26 10:56 作者: 上坡 時(shí)間: 2025-3-26 14:42 作者: 手榴彈 時(shí)間: 2025-3-26 16:46
Mamata Jafrin Mouli,Ishrat Islam,Marufa Khany to rapidly generate high-affinity protective antibody specificities. Detailed measurement of antibody affinity so far has largely been confined to studies of monoclonal antibodies (mAbs) and are laborious since each individual mAb needs to be evaluated in isolation. Here, we introduce two variants作者: 概觀 時(shí)間: 2025-3-26 23:54 作者: Feigned 時(shí)間: 2025-3-27 02:32
Danilo Palazzo,Frederick Steinerunctions. Production of the appropriate Ig class/subclass is critical for both successful host defense and avoidance of immunopathology. The assessment of an antigen-specific B cell response, including its magnitude and Ig class/subclass composition, is most often confined to the antibodies present 作者: anniversary 時(shí)間: 2025-3-27 07:39
https://doi.org/10.1007/978-3-642-88583-9ngle-cell level. However, traditional ELISPOT based on enzyme-substrate color development can only detect one target. Therefore, scientists developed multiple-target ELISPOT based on enzyme-substrate coloring. Besides, FluoroSPOT that can detect 2–4 fluorescent signals are developed. Nevertheless, t作者: 小樣他閑聊 時(shí)間: 2025-3-27 12:57 作者: Acclaim 時(shí)間: 2025-3-27 17:20 作者: 初學(xué)者 時(shí)間: 2025-3-27 21:44
Important Considerations for ELISpot Validation,ls in a straightforward and easy-to-learn approach. Its use in basic research, translational, and clinical work has been documented in countless publications. Harmonization guidelines and invaluable tools for optimal assay performance and evaluation exist. However, the validation of an established E作者: 遭遇 時(shí)間: 2025-3-27 22:15 作者: Badger 時(shí)間: 2025-3-28 03:57
An Overview of Peptides and Peptide Pools for Antigen-Specific Stimulation in T-Cell Assays,ISPOT), which depend on antigen-specific stimulation, increasingly use peptides to represent the antigen of interest. Besides single peptides, mixtures of peptides (peptide pools) are very frequently applied. Such peptide pools may, for example, represent entire proteins (with overlapping peptides c作者: obviate 時(shí)間: 2025-3-28 09:13
The Applications of ELISpot in the Identification and Treatment of Various Forms of Tuberculosis anidely used for the diagnosis of various infectious diseases, e.g., tuberculosis and sarcoidosis, and it is also widely used in cancer immunotherapy research. Its ability to distinguish between active and latent forms of tuberculosis makes it an extremely powerful tool for epidemiological studies and作者: Fibrin 時(shí)間: 2025-3-28 12:06 作者: 名字的誤用 時(shí)間: 2025-3-28 16:35
Comparing Flow Cytometry and ELISpot for Detection of IL-10, IL-6, and TNF Alpha on Human PBMCs,ion has its own advantages and challenges. ELISpot is more sensitive compared to ELISA and appears to be more consistent in detecting IL-10 production than flow cytometry. ELISpot can be used for detecting the secretion of multiple cytokines but not from the same cells simultaneously, whereas flow c作者: fatty-acids 時(shí)間: 2025-3-28 19:13
,Reagent Tracker? Platform Verifies and Provides Audit Trails for the Error-Free Implementation of Td cell material, such as peripheral blood mononuclear cells (PBMC). Establishing their frequency within all PBMC permits to assess the magnitude of antigen-specific T-cell immunity; the simultaneous measurement of their cytokine signatures reveals these T-cells’ lineage and effector functions, that 作者: 有毒 時(shí)間: 2025-3-29 00:51 作者: V切開(kāi) 時(shí)間: 2025-3-29 05:36
,Interferon-γ/IL-2 ELISpot and mRNA Responses to the SARS-CoV2, Feline Coronavirus Serotypes 1 (FCoV and 2 (FCoV2). Cats naturally infected with FCoV1 have been shown to possess serum reactivities with FCoV1 and SCoV2 RBDs but not with FCoV2 RBD. In the current study, COVID-19-vaccinated humans and FCoV1-infected laboratory cats were evaluated for interferon-gamma (IFNγ) and interleukin-2 (IL-2 EL作者: Obverse 時(shí)間: 2025-3-29 10:27 作者: Mets552 時(shí)間: 2025-3-29 14:36 作者: palette 時(shí)間: 2025-3-29 18:39 作者: 讓步 時(shí)間: 2025-3-29 21:48 作者: asthma 時(shí)間: 2025-3-30 03:46
Using PBMCs in a Multiplex FluoroSpot Assay for Detection of Innate Immune Response-Modulating Impue-Modulating Impurities (IIRMIs) in injectable drugs by activation of monocytes in human peripheral blood mononuclear cells (PBMCs). We describe the use of a triple-color IL-1β/IL-6/TNF-α FluoroSpot assay as a sensitive tool for quantification of the frequencies of IIRMI-activated monocytes as well 作者: Accolade 時(shí)間: 2025-3-30 08:01 作者: Emasculate 時(shí)間: 2025-3-30 11:16 作者: OVER 時(shí)間: 2025-3-30 15:58
Triple-Color FluoroSpot Analysis of Polyfunctional Antigen-Specific T Cells by Quantification of Spncies of spots derived from cells co-secreting multiple cytokines. Due to the fluorescent read-out signal, sophisticated reader instruments can also measure the relative spot volume, making it possible to differentiate between spots generated by cells secreting different levels of one or more cytoki作者: painkillers 時(shí)間: 2025-3-30 19:21 作者: CARE 時(shí)間: 2025-3-30 20:55 作者: 碎石 時(shí)間: 2025-3-31 04:33
Zhiming Cheng,Mark Wang,Junhua Chen way peptides are produced, they will always contain certain amounts of impurities such as peptides with deletions or truncated peptides, and there may be additional by-products of peptide synthesis. Optimized synthesis protocols as well as purification help reduce impurities that might otherwise ca作者: 混合 時(shí)間: 2025-3-31 07:19 作者: Water-Brash 時(shí)間: 2025-3-31 09:26 作者: Mortal 時(shí)間: 2025-3-31 17:26
rated the differences between ELISpot and flow cytometry by stimulating cells with two toll-like receptor (TLR) agonists, LPS or Pam.CSK.. Both activators increase production of various cytokines, including IL-10, IL-6, and TNF-alpha. The TLR2 antagonist, MMG-11, was used to inhibit this increased c作者: JECT 時(shí)間: 2025-3-31 21:07 作者: 錢(qián)財(cái) 時(shí)間: 2025-3-31 23:21
Urban Design and Representationaccination. However, her IL-2 responses to FCoV1 and FCoV2 RBDs were low but detectable at 10 mo post-last vaccination. This observation suggests that initially robust IFNγ responses to SCoV2 RBD may be an outcome of robust inflammatory IFNγ responses to SCoV2 RBD. Hence, the T-cell responses of vac作者: RENIN 時(shí)間: 2025-4-1 04:54
Pedro Telhado Pereira,Maria Eugénia Mataection and/or vaccination. Here, we describe in detail the methodology for using this ELISPOT assay to quantify SARS-CoV-2 spike-specific memory B cells produced by infection and/or vaccination in human PBMC samples. Application of this assay may help better understand and predict SARS-CoV-2 recall 作者: 符合規(guī)定 時(shí)間: 2025-4-1 08:59
https://doi.org/10.1057/9780230119581x vivo, revealing its immunoglobulin class/subclass utilization, and its affinity distribution for the original, and for variant viruses/antigens. Because such comprehensive B?cell ImmunoSpot. tests can be performed with minimal cell material, are scalable, and robust, they promise to be well-suited作者: induct 時(shí)間: 2025-4-1 10:11
