標(biāo)題: Titlebook: Handbook of ELISPOT; Methods and Protocol Alexander E. Kalyuzhny Book 2018Latest edition Springer Science+Business Media, LLC, part of Spri [打印本頁] 作者: 高出來的名詞 時間: 2025-3-21 18:31
書目名稱Handbook of ELISPOT影響因子(影響力)
書目名稱Handbook of ELISPOT影響因子(影響力)學(xué)科排名
書目名稱Handbook of ELISPOT網(wǎng)絡(luò)公開度
書目名稱Handbook of ELISPOT網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Handbook of ELISPOT被引頻次
書目名稱Handbook of ELISPOT被引頻次學(xué)科排名
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書目名稱Handbook of ELISPOT讀者反饋
書目名稱Handbook of ELISPOT讀者反饋學(xué)科排名
作者: 小平面 時間: 2025-3-21 21:32
Mastering the Computational Challenges of Elispot Plate Evaluation, addressing the analysis step, the plate evaluation. Comparing that fact with the vast amount of analysis advise available for other single cell immune assay, for example, intracellular cytokine staining, the overall impression may be that Elispot evaluation is just simple enough to not require exte作者: Alveolar-Bone 時間: 2025-3-22 01:15 作者: 一回合 時間: 2025-3-22 07:01
Automatic Search of Spots and Color Classification in ELISPOT Assay,their morphological variations make it difficult developing a reliable software application. An image recognition method allowing the automatic detection and classification of round objects (spots) on ELISPOT images independently of the registration conditions was developed. The emphasis is done on 作者: incontinence 時間: 2025-3-22 10:43
,Four Color ImmunoSpot? Assays for Identification of Effector T-Cell Lineages,FN-γ in response to antigen exposure, these assays permit the measurement of the frequency of antigen-specific T cells among white blood cells. These assays therefore are well suited to assess clonal expansions, that is, whether a (Th1) T cell response has been induced to an antigen in a test subjec作者: 匍匐前進 時間: 2025-3-22 14:43
Detection of Cross-Reactive B Cells Using the FluoroSpot Assay,ingle antigen. By reversing the B cell ELISpot and using anti-IgG capture antibodies instead of coated antigen, the specificity of antibodies secreted by B cells can be defined using soluble tagged antigen for detection. When combining this approach with fluorescent detection of the antigen in a B c作者: PANG 時間: 2025-3-22 20:35
,Multiplex ImmunoSpot? Assays for the Study of Functional B Cell Subpopulations,of effector molecules such as cytokines, chemokines, and granzyme. B cell subpopulations releasing such effector molecules have been implicated in immunobiology and a number of diseases..Unlike antigen-specific T cells that can be identified by multimer staining, and then counter-stained to define T作者: 命令變成大炮 時間: 2025-3-22 22:59 作者: 贊成你 時間: 2025-3-23 03:28
Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpotUnique single cell resolution along with high throughput potential sets ELISPOT apart from flow cytometry, ELISA, microarray- and bead-based multiplex assays. The necessity to unambiguously identify individual T and B cells that do, or do not co-express certain analytes, including polyfunctional cyt作者: arthroplasty 時間: 2025-3-23 05:38
,Multi-Color FLUOROSPOT Counting Using ImmunoSpot? Fluoro-X? Suite, have recently become a major new avenue of development of ELISPOT technology. Advances in assay techniques and the availability of commercial test kits stimulated development of multi-color FLUOROSPOT data analysis platforms. The ImmunoSpot. Fluoro-X? Software Suite was developed by CTL as an integ作者: synovitis 時間: 2025-3-23 13:30
B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Bloomunological research as compared to the surgically excised and biopsied lymphoid specimens. To determine the functional status of immune system from peripheral blood, the enzyme-linked immunospot (ELISpot) assay is a popular method of choice owing to its high sensitivity, great accuracy, and easy pe作者: labile 時間: 2025-3-23 16:28 作者: 費解 時間: 2025-3-23 19:44 作者: 令人苦惱 時間: 2025-3-23 22:30
Cultured ELISpot Assay to Investigate Dengue Virus Specific T-Cell Responses,The use of an in vitro expansion step before the ELISpot distinguishes such “cultured” ELISpots from “ex vivo” ELISpots. Cultured ELISpots have the advantage that lower frequency responses can be analyzed compared to ex vivo ELISpots, but do carry the associated potential distortions of the expansio作者: 聾子 時間: 2025-3-24 04:46 作者: Synapse 時間: 2025-3-24 06:32
Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection,ulation with flow cytometry in functionality studies is challenging. iNKT cells are known to produce both Th1 and Th2 cytokines immediately upon antigen stimulation. Therefore, we have used an ELISpot assay to determine the production of IFN-? and IL-4 after stimulation with KRN7000, which has shown作者: 改良 時間: 2025-3-24 11:10 作者: AMITY 時間: 2025-3-24 15:04
Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccine, as heterologous FIV strains from the vaccine subtypes with closely related envelope (Env) sequences. Such protection was mediated by the FIV neutralizing antibodies (NAbs) induced by the vaccines. Remarkably, both prototype and commercial FIV vaccines also conferred protection against heterologous 作者: 高射炮 時間: 2025-3-24 21:14 作者: Deceit 時間: 2025-3-25 02:45 作者: Apogee 時間: 2025-3-25 05:00
Domenica Di Costanzo,Mariano Mazza addressing the analysis step, the plate evaluation. Comparing that fact with the vast amount of analysis advise available for other single cell immune assay, for example, intracellular cytokine staining, the overall impression may be that Elispot evaluation is just simple enough to not require exte作者: jettison 時間: 2025-3-25 10:26
Phrenic Nerve Stimulation in Central Apnea,caused by different factors including cell- and immunoassay-related issues. In our study, we have shown that nonspecific spots can result from either cells or their debris sticking to the membranes in ELISpot plates, as well as by impurities in wash buffers and precipitation of aggregated detection 作者: DEI 時間: 2025-3-25 14:42 作者: 模范 時間: 2025-3-25 17:00 作者: 極小 時間: 2025-3-25 23:44 作者: aerial 時間: 2025-3-26 01:12
Upper Tract Urothelial Carcinomaof effector molecules such as cytokines, chemokines, and granzyme. B cell subpopulations releasing such effector molecules have been implicated in immunobiology and a number of diseases..Unlike antigen-specific T cells that can be identified by multimer staining, and then counter-stained to define T作者: 古董 時間: 2025-3-26 05:04 作者: 做方舟 時間: 2025-3-26 09:00 作者: Employee 時間: 2025-3-26 12:57 作者: Banquet 時間: 2025-3-26 17:13 作者: 先兆 時間: 2025-3-26 21:55 作者: unstable-angina 時間: 2025-3-27 02:34
Upscaling Multiphase Flow in Porous Media a sense of urgency in the field to further define the genesis and to more fully describe the immune response. The recent explosive epidemics of Zika in the Americas and the co-circulation of ZIKV with the phylogenetically similar DENV has raised important questions and concerns regarding the role o作者: TIGER 時間: 2025-3-27 08:28 作者: sleep-spindles 時間: 2025-3-27 11:58 作者: BROOK 時間: 2025-3-27 15:17
Other Important Upwelling Systems,ulation with flow cytometry in functionality studies is challenging. iNKT cells are known to produce both Th1 and Th2 cytokines immediately upon antigen stimulation. Therefore, we have used an ELISpot assay to determine the production of IFN-? and IL-4 after stimulation with KRN7000, which has shown作者: Instinctive 時間: 2025-3-27 18:36
Stanley Osher,Sukumar Chakravarthys challenge in non-human primates. Generally, T cell responses can be investigated with peptides. However, hundreds of over-lapping peptides need to be synthesized to cover the whole sequence of a protein, which brings the cost up to a much higher level than purchasing a protein. We have developed a作者: febrile 時間: 2025-3-27 22:28 作者: FADE 時間: 2025-3-28 04:49
https://doi.org/10.1007/978-3-662-12506-9. In addition to antibody titers, the reliable measurement of these cells in human blood (peripheral blood mononuclear cells/PBMCs or purified B cells) provides valuable information on the preparedness of the adaptive immune system to respond to infection or vaccines, and potentially supports the di作者: saphenous-vein 時間: 2025-3-28 07:29
Mastering the Computational Challenges of Elispot Plate Evaluation,port was published about the subjectivity and operator-dependency of plate evaluation even when using automated reader systems (Janetzki et al., J Immunol Methods 291, 175–183, 2004). Later, the plate evaluation was identified as a main factor causing variability in Elispot results, triggering the i作者: Antioxidant 時間: 2025-3-28 11:55
,Four Color ImmunoSpot? Assays for Identification of Effector T-Cell Lineages, cells, stem-cell like CD8 memory cells, dysfunctional- and senescent CD8 cells all differ in the extent they produce these molecules upon antigen re-encounter. We therefore have developed, and introduce here, a four color T cell ELISPOT assay in which the co-expression levels of IFN-γ, IL-2, TNF-α,作者: 船員 時間: 2025-3-28 14:34
Detection of Cross-Reactive B Cells Using the FluoroSpot Assay,s provides a tool for a single-cell analysis of B cell cross-reactivity, for example, with variable and polymorphic antigens found in various pathogens; or analysis of other types of immune responses where analysis of cross-reactivity is of interest. It is also possible to simultaneously analyze B c作者: 雀斑 時間: 2025-3-28 20:41
,Multiplex ImmunoSpot? Assays for the Study of Functional B Cell Subpopulations,ction and the secretion of other secretory B cell products, it would then be possible to identify B cell subpopulations that co-express effector molecules. Here we introduce multiplex ELISPOT assays in which measurements of antibody secretion are combined with the detection of Granzyme B, IL-6, IL-1作者: Nerve-Block 時間: 2025-3-29 01:07
,Detecting all Immunoglobulin Classes and Subclasses in a Multiplex 7 Color ImmunoSpot? Assay,ntigen-specific B cells producing IgM, IgA, IgE, IgG., IgG., IgG., and IgG.. We report here the development of a multiplex seven color B cell ImmunoSpot. assay in which the number of antigen-specific B cells can be established simultaneously for all major antibody classes and subclasses, requiring t作者: 記成螞蟻 時間: 2025-3-29 03:40 作者: Formidable 時間: 2025-3-29 10:37
,Multi-Color FLUOROSPOT Counting Using ImmunoSpot? Fluoro-X? Suite,rated quality control module, with optional GLP and CFR Part 11 compliant package and role-based security, enables data validation, review, and approval with complete audit trails. The extensive multi-format data output and presentation capabilities of the Fluoro-X? software allow further analysis o作者: 被詛咒的人 時間: 2025-3-29 15:22
B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Bloossay variability amongst samples from different sources. Here we introduce methods to isolate human peripheral blood mononuclear cells (PBMCs) for quantification of the antigen-specific antibody-secreting cells using the ELISpot assay.作者: 障礙 時間: 2025-3-29 18:01 作者: 雪崩 時間: 2025-3-29 23:06
Cultured ELISpot Assay to Investigate Dengue Virus Specific T-Cell Responses,e Japanese encephalitis virus nor other flaviviruses. Therefore, this assay is likely to be useful in determining the past infecting DENV serotype in immune-epidemiological studies and in dengue vaccine trials.作者: BIAS 時間: 2025-3-30 01:56 作者: 潔凈 時間: 2025-3-30 06:12 作者: 吞沒 時間: 2025-3-30 10:35 作者: BOOR 時間: 2025-3-30 13:45 作者: 百科全書 時間: 2025-3-30 17:00
Domenica Di Costanzo,Mariano Mazzaport was published about the subjectivity and operator-dependency of plate evaluation even when using automated reader systems (Janetzki et al., J Immunol Methods 291, 175–183, 2004). Later, the plate evaluation was identified as a main factor causing variability in Elispot results, triggering the i作者: persistence 時間: 2025-3-30 23:09
Stephen R. Rotman,John R. Saltzman cells, stem-cell like CD8 memory cells, dysfunctional- and senescent CD8 cells all differ in the extent they produce these molecules upon antigen re-encounter. We therefore have developed, and introduce here, a four color T cell ELISPOT assay in which the co-expression levels of IFN-γ, IL-2, TNF-α,作者: octogenarian 時間: 2025-3-31 01:03 作者: Colonoscopy 時間: 2025-3-31 06:18 作者: 有常識 時間: 2025-3-31 09:29 作者: gnarled 時間: 2025-3-31 15:44
Raquel E. Aldana,Josephine M. Morenotecting individual cells that secrete multiple analytes vs. random overlays of spots. Here, we present a theoretical and experimental validation study for three and four color T- and B-cell FLUOROSPOT data analysis. The ImmunoSpot. Fluoro-X? analysis system we used includes an automatic image acquis作者: 休閑 時間: 2025-3-31 18:11
Mental Health Aspects of Voluntary Migrationrated quality control module, with optional GLP and CFR Part 11 compliant package and role-based security, enables data validation, review, and approval with complete audit trails. The extensive multi-format data output and presentation capabilities of the Fluoro-X? software allow further analysis o作者: 雄辯 時間: 2025-3-31 23:57
https://doi.org/10.1007/978-1-4684-3794-2ssay variability amongst samples from different sources. Here we introduce methods to isolate human peripheral blood mononuclear cells (PBMCs) for quantification of the antigen-specific antibody-secreting cells using the ELISpot assay.
