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標(biāo)題: Titlebook: Handbook of Capillary Electrophoresis Applications; Hideharu Shintani (Section Director),J. Polonsky ( Book 1997 Chapman & Hall 1997 bioch [打印本頁]

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Handbook of Capillary Electrophoresis Applications
作者: 反復(fù)拉緊    時間: 2025-3-22 12:20
Hideharu Shintani (Section Director),J. Polonsky (
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Handbook of Capillary Electrophoresis Applications978-94-009-1561-9
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http://image.papertrans.cn/h/image/420941.jpg
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Alexander Schmitt,Wolfgang MinkerIn capillary electrophoresis, a small amount of sample solution is injected from the inlet end of a capillary and a high voltage is applied between the two ends of the capillary, both of which are immersed in electrolyte. Satisfactory reproducibility of the injection volume is required for quantitative analysis.
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Experimental Vehicles as Development ToolsHigh performance liquid chromatography (HPLC) is a most important technique for general purposes and has a potential market in the field of analytical chemistry. The principles and theory of HPLC can be partially applied to CE. The comparison of CE with HPLC provides a useful index in considering a future development plan in CE.
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Sample injectionIn capillary electrophoresis, a small amount of sample solution is injected from the inlet end of a capillary and a high voltage is applied between the two ends of the capillary, both of which are immersed in electrolyte. Satisfactory reproducibility of the injection volume is required for quantitative analysis.
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Amino acidsSeveral analytical methods have been reported for standard or derivatized amino acids using combinations of several capillary electrophoresis modes. This chapter will present several of these current methods and explain exactly how this technique is applied. These applications are summarized in Table 12.1.
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The Open Door to Foreign Capital,tion constants in affinity CE, the molecular weight in molecular sieving mode, isoelectric point (pI) in capillary isoelectric focusing (CIEF), etc. These two pieces of information, the peak area/height and the migration time, are described in this chapter.
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Towards Cultural Psychology of Religionadvancement. In this chapter, recent developments in column technology are described including how to control EOF, the method of coating the surface of inner wall of the capillary, gel-filled columns, connection of capillaries and design of capillary shape.
作者: 招惹    時間: 2025-3-25 07:06
Ayesha Kashif,Stephane Ploix,Julie Dugdale conventional on-line UV detection. Several studies have been carried out to enhance the detection sensitivity, e.g. sample preconcentration, choice of detection system including strong light source, a detector with high sensitivity and efficient optical alignment, and derivatization of analytes [1].
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Remco Hoogma,Matthias Weber,Boelie Elzen inexact collection and, in the cases where the collection vials contain a small volume of buffer, gives dilution of the fraction. The first problem is solved by calculating the migration time for fraction collection corresponding to the related migration behavior [1].
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Quantitative analysis, precision and reproducibilitytion constants in affinity CE, the molecular weight in molecular sieving mode, isoelectric point (pI) in capillary isoelectric focusing (CIEF), etc. These two pieces of information, the peak area/height and the migration time, are described in this chapter.
作者: 漂浮    時間: 2025-3-25 23:07
Column technologyadvancement. In this chapter, recent developments in column technology are described including how to control EOF, the method of coating the surface of inner wall of the capillary, gel-filled columns, connection of capillaries and design of capillary shape.
作者: 起來了    時間: 2025-3-26 01:30
Detection conventional on-line UV detection. Several studies have been carried out to enhance the detection sensitivity, e.g. sample preconcentration, choice of detection system including strong light source, a detector with high sensitivity and efficient optical alignment, and derivatization of analytes [1].
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Analysis of erythropoietin by capillary electrophoresishe first functional recombinant human erythropoietin was cloned, expressed [6] and made available as one of the most celebrated therapeutic drugs in recent years. Recombinant EPO (r-EPO) and natural EPO purified from the urine showed no significant difference in their functional activity . or their carbohydrate structures [7, 8].
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https://doi.org/10.1007/978-94-017-3558-2m dodecyl sulfate)—polyacrylamide gel electrophoresis (SDS-PAGE) or isoelectric focusing (IEF) can also be carried out by CE as capillary gel electrophoresis (CGE) or capillary isoelectric focusing (CIEF).
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IPR Management to Business Management,ly high such that on a molar basis, they can catalyze the transformation of 10. to 10. moles of the substrate per minute. Enzymes provide some of the best chemical amplification processes in nature which have been used in many practical applications, including enzyme assay, enzyme-linked immunoassay (ELISA) and DNA probe assays.
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Introduction and Lukacs have improved the technique substantially with capillaries of inner diameter less than 100μm [4–6]. They demonstrated the characteristics of modern CE, i.e. the sufficient dissipation of heat resulting from high applied voltage, on-column detection and the high-efficiency separation.
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Ruslan Grinberg,Herbert Brückerand capillary isoelectric focusing (CIEF) modes may be used to determine qualitative data but the most generally used CE techniques for performing quantitative analysis of analytes are capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC).
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Jan Nagler,Jeroen van den Hoven,Dirk Helbingfor electrolytes in CE are as follows; (1) sufficient buffering capacity to suppress pH shift caused by migration of ions in the electrolyte, (2) low electric conductivity to avoid heat generation caused by high current, (3) low absorbance in the wavelength of detection not to disturb the on-column UV detection of the analyte zone.
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Is India Key to Asian Financial Integration?e-zone electrophoresis in a narrow-bore tube of 3 mm inner diameter [1]. His pioneering work was followed by Virtanen [2] and Mikkers . [3]. Jorgenson and Lukacs have improved the technique substantially with capillaries of inner diameter less than 100μm [4–6]. They demonstrated the characteristics
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The Open Door to Foreign Capital,n in the detection zone which corresponds to the sample mass in the objective zone. The other is the migration time which generally represents the qualitative characteristics of the sample. Quantitative indices can be derived from the migration time in certain measuring systems, dissociation/associa
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Jan Nagler,Jeroen van den Hoven,Dirk Helbing in CE corresponds both to the stationary phase and to the eluting solution in high performance liquid chromatography (HPLC). Features worthy of note for electrolytes in CE are as follows; (1) sufficient buffering capacity to suppress pH shift caused by migration of ions in the electrolyte, (2) low
作者: Offbeat    時間: 2025-3-30 02:14
Ayesha Kashif,Stephane Ploix,Julie Dugdaley low. Since the capillary diameter in CE is less than 100μm and the entire volume of the capillary in most cases is less than 10 μl, the optical path should be short enough to detect analyte zones without loss of separation efficiency. Consequently, the detection limit is between 1 and 0.1 ppm with
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Brian Fitzgerald,Audris Mockus,Minghui Zhou on CE, only 120 had been published. They stated ‘During the last decade the number of scientific papers published in this field has grown exponentially’ [1]. Expansion in publication has continued. Now, only five years after their review, the total number of the articles published in 1994 was nearl
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Shampa Chakraverty,Anil Goel,Sanjay Misraels. An additional miniaturization has been demonstrated by the use of photolithographically fabricated microstructure by Ciba Geigy [1, 2]. Photolithographic patterning on the silicon wafer has become a well-known technique in microelectronics and has been applied to gas and liquid chromatography.
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https://doi.org/10.1007/978-1-4020-8962-6of biosynthesis is in the liver in the fetus and switches to the kidney in adults [2–4]. It is maintained in circulation at about 10 pM under normal physiological conditions, and may be excreted in urine [5]. EPO was first purified in small quantities from the urine of aplatic anemia patients [5]. T
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IPR Management to Business Management,bstantial part of modern biochemistry and molecular biology is devoted to their study. Nearly all biochemical reactions occur in complex mixtures with great speed through the mediation of enzymes with a high degree of specificity and great efficiency. In contrast to an antibody which binds specifica
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Column technology. The other is as a pump, the silanol groups on its inner surface yielding electroosmotic flow (EOF). Remarkable progress has been made in making improvements in capillaries to achieve high quality and uniformity in CE. However, more improvements in several properties of capillaries are for further
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Electrolyte systems in CE corresponds both to the stationary phase and to the eluting solution in high performance liquid chromatography (HPLC). Features worthy of note for electrolytes in CE are as follows; (1) sufficient buffering capacity to suppress pH shift caused by migration of ions in the electrolyte, (2) low
作者: 偶然    時間: 2025-4-1 09:02
Detectiony low. Since the capillary diameter in CE is less than 100μm and the entire volume of the capillary in most cases is less than 10 μl, the optical path should be short enough to detect analyte zones without loss of separation efficiency. Consequently, the detection limit is between 1 and 0.1 ppm with




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