標題: Titlebook: Handbook of Biological Confocal Microscopy; James B. Pawley Book 19952nd edition Springer-Verlag US 1995 Confocal.Microscopy.Pawley.biolog [打印本頁] 作者: Entangle 時間: 2025-3-21 18:38
書目名稱Handbook of Biological Confocal Microscopy影響因子(影響力)
書目名稱Handbook of Biological Confocal Microscopy影響因子(影響力)學(xué)科排名
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書目名稱Handbook of Biological Confocal Microscopy網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Handbook of Biological Confocal Microscopy被引頻次
書目名稱Handbook of Biological Confocal Microscopy被引頻次學(xué)科排名
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書目名稱Handbook of Biological Confocal Microscopy年度引用學(xué)科排名
書目名稱Handbook of Biological Confocal Microscopy讀者反饋
書目名稱Handbook of Biological Confocal Microscopy讀者反饋學(xué)科排名
作者: 晚間 時間: 2025-3-21 22:35
Visualization Systems for Multidimensional CLSM Images,e are available from confocal microscope manufacturers, third-party vendors, and other microscopists. The author has attempted to collate important techniques used in these programs. Sources and approximate cost of example systems are given in Table 1.作者: 溝通 時間: 2025-3-22 00:55
Fluorophores for Confocal Microscopy, a century ago (in the case of fluoresceins or rhodamines) or several billion years ago (in the case of phycobiliproteins). Moreover, whereas competition between commercial makers of confocal microscopes stimulates ardent efforts to refine the instrumentation, relatively few companies or academic sc作者: heterogeneous 時間: 2025-3-22 05:01
General Aspects of Thoracic Anesthesiaeld. We may also produce images in which object height is coded as brightness, or combine the whole data set to provide an isometric view of the object (Fig. 1). It is also possible to use false color to label features of interest or, by simple processing, to obtain stereoscopic pairs (van der Voort作者: MUTED 時間: 2025-3-22 09:12 作者: Adulterate 時間: 2025-3-22 12:54 作者: Flatus 時間: 2025-3-22 18:25
Book 19952nd editionf, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D F作者: 手工藝品 時間: 2025-3-23 00:48
Francis C. Wells,Aman S. Coonarpletely developed. Computational image processing provides a powerful approach for reducing the systematic errors present in any 3D data set and enhancing the clarity and contrast of relevant features.作者: Obituary 時間: 2025-3-23 04:24 作者: 公式 時間: 2025-3-23 09:09
Gabriel C. Tender MD,David G. Kline MDeral other chapters in this volume present additional specific information concerning some of these optical components (e.g., light sources, intermediate optics, objective lenses). Our purpose is to treat the illumination path as an integral system and describe its contribution to the overall performance of the confocal microscope.作者: canvass 時間: 2025-3-23 10:02
Artefacts in Thoracic Ultrasoundd or dormant, because it takes time to obtain a 3D data set, and the image will be spoiled if the object moves or changes shape during that time. Now, however, fast disk-scanning TSMs with flat spectrum sources and special objective lenses with linear chromatic dispersion (LCD) can provide 3D data in which height is coded in color, at video rate.作者: 無法取消 時間: 2025-3-23 15:21 作者: semble 時間: 2025-3-23 21:11 作者: Kaleidoscope 時間: 2025-3-24 01:15
https://doi.org/10.1057/9780230005808losely a given instrument performs. Additional information to help the user to operate the confocal microscope in an optimal manner can be found in Chapter 36, “Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen,” which also includes methods for measuring resolution.作者: floaters 時間: 2025-3-24 02:59 作者: SPECT 時間: 2025-3-24 07:41 作者: 勉勵 時間: 2025-3-24 11:50 作者: 消滅 時間: 2025-3-24 16:03 作者: Efflorescent 時間: 2025-3-24 20:25 作者: 返老還童 時間: 2025-3-25 00:58 作者: 琺瑯 時間: 2025-3-25 05:38
cope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxi作者: 溫和女人 時間: 2025-3-25 09:29
Thomas Reid’s Lectures on the Fine Artsen running parallel to, the line of sight; penetrate deep light-scattering tissues; gain impressive three-dimensional (3D) views at very high resolution; obtain differential interference or phase-contrast images in exact register with confocal fluorescence images; and improve the precision of microphotometry.作者: CANT 時間: 2025-3-25 13:53
John Finnis and the New Natural Law Theory, we don’t “cook” the sample by oversampling. The material described here is generally applicable to all imaging systems, but is of particular import for the flexible digital images available from a confocal laser scanning microscope (CLSM).作者: Promotion 時間: 2025-3-25 17:22
F. W. Schildberg,E. Kiffner,M. H. Schoenbergt disciplines as far afield as physiology, spectroscopy, fluorescence lifetime analysis, and even DNA sequencing. As a result, the requirements and design constraints on appropriate detection systems are as varied as the fields to which the technique is applied.作者: SHRIK 時間: 2025-3-25 21:40 作者: 巫婆 時間: 2025-3-26 01:59 作者: capsule 時間: 2025-3-26 06:22
Foundations of Confocal Scanned Imaging in Light Microscopy,en running parallel to, the line of sight; penetrate deep light-scattering tissues; gain impressive three-dimensional (3D) views at very high resolution; obtain differential interference or phase-contrast images in exact register with confocal fluorescence images; and improve the precision of microphotometry.作者: 冥界三河 時間: 2025-3-26 09:10
The Pixilated Image, we don’t “cook” the sample by oversampling. The material described here is generally applicable to all imaging systems, but is of particular import for the flexible digital images available from a confocal laser scanning microscope (CLSM).作者: NOCT 時間: 2025-3-26 12:43
Photon Detectors for Confocal Microscopy,t disciplines as far afield as physiology, spectroscopy, fluorescence lifetime analysis, and even DNA sequencing. As a result, the requirements and design constraints on appropriate detection systems are as varied as the fields to which the technique is applied.作者: Cloudburst 時間: 2025-3-26 16:49
Image Contrast in Confocal Light Microscopy,ive or print. In other words, contrast is the difference in signal strength between various parts of an image or between details of interest and “background.” In science, the contrast is proportional to the intensity difference (Δ.) between two image areas, divided by the average image brightness ..作者: frivolous 時間: 2025-3-27 00:39
Guiding Principles of Specimen Preservation for Confocal Fluorescence Microscopy, react with electron-dense stains deposited in fixed specimens. Immunogold labeling permits the study of non-electron-dense material, but EM sections must still be very thin to avoid problems with the penetration of the labeled antibodies and to reduce scattering of the electron beam.作者: 招募 時間: 2025-3-27 01:55 作者: Ptosis 時間: 2025-3-27 08:33
Foundations of Confocal Scanned Imaging in Light Microscopy,he new microscope, one can slice incredibly clean thin optical sections out of thick fluorescent specimens; view specimens in planes tilted to, and even running parallel to, the line of sight; penetrate deep light-scattering tissues; gain impressive three-dimensional (3D) views at very high resoluti作者: 星星 時間: 2025-3-27 12:19
Fundamental Limits in Confocal Microscopy,y the use of confocal sampling techniques. This chapter will discuss the fundamental considerations that limit the performance of all confocal microscopes. Though at present no commercially available equipment approaches these limits, some simple tests will be described to help the user assess how c作者: Frequency-Range 時間: 2025-3-27 16:16 作者: bacteria 時間: 2025-3-27 17:49 作者: humectant 時間: 2025-3-27 22:07 作者: 揮舞 時間: 2025-3-28 03:18 作者: PALL 時間: 2025-3-28 07:37
The Specimen Illumination Path and Its Effect on Image Quality,n) optical paths contribute equally to the formation of the final image. However, many of the optical and imaging properties of confocal microscopes can be analyzed effectively by considering the illumination and detection paths to be two distinct entities. In this chapter, we explore one of these o作者: 規(guī)章 時間: 2025-3-28 10:49 作者: Ballad 時間: 2025-3-28 16:08
Intermediate Optics in Nipkow Disk Microscopes, optical cross-sectioning. Its transverse resolution, ., and the contrast of the image are better than with a standard microscope, and because it uses a laser beam, the illumination intensity is very high. At the same time, a choice of illumination wavelengths is available (Wilson and Sheppard, 1984作者: NOMAD 時間: 2025-3-28 20:31
The Role of the Pinhole in Confocal Imaging System,on) and optical sectioning (Wilson and Sheppard, 1984). It is probably the latter property that is most useful, as it gives rise to the ability to image a thick specimen in three dimensions. This is possible because the optical system detects information only from a thin region in the neighborhood o作者: 疲憊的老馬 時間: 2025-3-29 01:37
Photon Detectors for Confocal Microscopy,ect in the absence of interfering information from neighboring points. Recently, confocal techniques have expanded to encompass not only morphology but disciplines as far afield as physiology, spectroscopy, fluorescence lifetime analysis, and even DNA sequencing. As a result, the requirements and de作者: AXIOM 時間: 2025-3-29 05:39
The Collection, Processing, and Display of Digital Three-Dimensional Images of Biological Specimensmade it possible to record 3D microscopic images of biological specimens using either electron or light microscopy. While the collection of 3D data sets has now become routine, the analysis and interpretation of these images generally require significant time and effort. This is true, in part, becau作者: notice 時間: 2025-3-29 10:07
Visualization Systems for Multidimensional CLSM Images,ations involving computer-generated models of macroscopic objects. These methods have been adapted for biological visualization of mainly tomographic medical images and serial section data (e.g., Cookson ., 1989; review: Cookson, 1994). Most algorithms were not devised specifically for microscopy da作者: BLINK 時間: 2025-3-29 13:58 作者: BLOT 時間: 2025-3-29 16:16
Fluorophores for Confocal Microscopy,fic than absorbance or reflectance, and because it works so well with epi-illumination, which greatly simplifies scanner design. These advantages of fluorescence are critically dependent on the availability of suitable fluorophores that can either be tagged onto biological macromolecules to show the作者: 跳脫衣舞的人 時間: 2025-3-29 22:24
Image Contrast in Confocal Light Microscopy,ntrast mechanism by which to “see” the structures of interest. As defined by the ., contrast is the difference between light and dark areas of a negative or print. In other words, contrast is the difference in signal strength between various parts of an image or between details of interest and “back作者: 抒情短詩 時間: 2025-3-29 23:56
Guiding Principles of Specimen Preservation for Confocal Fluorescence Microscopy, molecular data with morphology. Electron microscopy (EM) provides fine ultrastructural detail but is limited to the study of cellular structures that react with electron-dense stains deposited in fixed specimens. Immunogold labeling permits the study of non-electron-dense material, but EM sections 作者: 有毒 時間: 2025-3-30 06:55 作者: 埋葬 時間: 2025-3-30 10:58
Thomas Reid’s Lectures on the Fine Artshe new microscope, one can slice incredibly clean thin optical sections out of thick fluorescent specimens; view specimens in planes tilted to, and even running parallel to, the line of sight; penetrate deep light-scattering tissues; gain impressive three-dimensional (3D) views at very high resoluti作者: abduction 時間: 2025-3-30 12:47
https://doi.org/10.1057/9780230005808y the use of confocal sampling techniques. This chapter will discuss the fundamental considerations that limit the performance of all confocal microscopes. Though at present no commercially available equipment approaches these limits, some simple tests will be described to help the user assess how c作者: EVEN 時間: 2025-3-30 17:21
https://doi.org/10.1007/978-3-476-98873-7 minimal loss of image-forming signal. Significant enhancement of both axial and lateral imaging resolution is also available but only with substantial signal loss. Because of these optical advantages, the confocal laser scanning microscope (CLSM) can clearly image thin optical sections from within 作者: 不近人情 時間: 2025-3-31 00:07 作者: Aprope 時間: 2025-3-31 01:11
Concise Key to the Major Groupings,teristics of lasers in relation to their use as an illumination source. Lasers have a number of unique properties compared to other sources emitting electromagnetic radiation, such as arc lamps, which make them an almost ideal light source for use in confocal microscopy. These properties are:作者: consolidate 時間: 2025-3-31 08:52
Sivasubramanian Srinivasan,Ashish Chawlacontrast at which this detail is presented, the depth through the object from which useful information can be derived and the diameter of the useful field are all limited by the performance of the objective. All other imaging components, such as relay optics, Telan systems, tube lenses and eyepieces作者: tariff 時間: 2025-3-31 11:45 作者: 半導(dǎo)體 時間: 2025-3-31 14:27
Richard J. Sanders,Dean M. Donahue geometrical optics. Therefore, the author does not present unnecessary mathematics. The simple calculations that are part of the text should be sufficient to understand which calculations are relevant for the design of confocal microscopes.作者: output 時間: 2025-3-31 20:03 作者: 全國性 時間: 2025-3-31 22:49 作者: 平常 時間: 2025-4-1 03:12 作者: –吃 時間: 2025-4-1 09:50
