標(biāo)題: Titlebook: HIV Protocols; Nelson L. Michael,Jerome H. Kim Book 19991st edition Humana Press 1999 [打印本頁] 作者: 熱情美女 時間: 2025-3-21 20:08
書目名稱HIV Protocols影響因子(影響力)
書目名稱HIV Protocols影響因子(影響力)學(xué)科排名
書目名稱HIV Protocols網(wǎng)絡(luò)公開度
書目名稱HIV Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱HIV Protocols被引頻次
書目名稱HIV Protocols被引頻次學(xué)科排名
書目名稱HIV Protocols年度引用
書目名稱HIV Protocols年度引用學(xué)科排名
書目名稱HIV Protocols讀者反饋
書目名稱HIV Protocols讀者反饋學(xué)科排名
作者: 來自于 時間: 2025-3-21 23:23 作者: Generator 時間: 2025-3-22 00:49
Rapid Identification of Cloned HIV-1 Fragmentsntification, vectors containing the lacZ promoter and a partial . gene encoding the α-fragment of β-galactosidase were developed (pUC vectors) (.,.). Upon induction by IPTG (isopropyl-β-.-thio-galactopyranoside), the expressed β-galactosidase could cleave X-gal (5-bromo-4-choloro-3-indoyl-β-.-galact作者: 解凍 時間: 2025-3-22 05:33
Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter esignated A through H, have been identified (.,.). More recently, a ninth subtype, I, has been detected (.), as well as several highly divergent, or “outlying” variants of HIV-1 that have been tentatively classified as group O (.,.). This subtyping is based on a relatively small number of specimens 作者: enumaerate 時間: 2025-3-22 10:54 作者: 繁忙 時間: 2025-3-22 13:15
Book 19991st editionIV infection. HIVProtocols reflects the state of HIV research in several ways. First, chapters are organized into four sections: Virology, Molecular Biology, Humoral Immunology, and Cellular Immunology. This organization is a natural consequence of the diverse scientific disciplines that have been a作者: 琺瑯 時間: 2025-3-22 19:26
https://doi.org/10.1007/978-3-030-57473-4ted “R5,” whereas syncytium-inducing viruses preferentially utilize CXCR4, but may be dual tropic and are designated either “X4” or X4R5, respectively (.,.). Some viruses also appear to be able to utilize chemokine receptors CCR2B and CCR3 (.–.). In addition, the role of chemokine coreceptors in the作者: conference 時間: 2025-3-22 23:12 作者: tenuous 時間: 2025-3-23 05:11
https://doi.org/10.1007/978-94-6300-654-5ntification, vectors containing the lacZ promoter and a partial . gene encoding the α-fragment of β-galactosidase were developed (pUC vectors) (.,.). Upon induction by IPTG (isopropyl-β-.-thio-galactopyranoside), the expressed β-galactosidase could cleave X-gal (5-bromo-4-choloro-3-indoyl-β-.-galact作者: OTHER 時間: 2025-3-23 06:44
Gagari Chakrabarti,Chitrakalpa Senesignated A through H, have been identified (.,.). More recently, a ninth subtype, I, has been detected (.), as well as several highly divergent, or “outlying” variants of HIV-1 that have been tentatively classified as group O (.,.). This subtyping is based on a relatively small number of specimens 作者: 開始發(fā)作 時間: 2025-3-23 12:36
https://doi.org/10.1007/978-3-642-59246-1he core promoter elements by the viral Tat protein is located within the R region of the LTR at positions +1 to +59 (.,.). This transactivating region (TAR) folds into alternate RNA stem-loop structures that are found in the 5′ termini of all viral transcripts. The TAR element mediates a substantial作者: corpuscle 時間: 2025-3-23 16:31 作者: Instrumental 時間: 2025-3-23 21:50 作者: forecast 時間: 2025-3-24 00:00
Methods in Molecular Medicinehttp://image.papertrans.cn/h/image/420198.jpg作者: 狂亂 時間: 2025-3-24 03:26
culture assay based on the detection of HIV in patient peripheral blood mononuclear cells (PBMC) by the appearance of p24 gag protein in the culture supernatant. It can be used in a research setting as a measure of viral burden because the number of patient cells required to produce a positive culture is indicative of cellular viral load.作者: 效果 時間: 2025-3-24 09:22
Quantitative HIV Culture culture assay based on the detection of HIV in patient peripheral blood mononuclear cells (PBMC) by the appearance of p24 gag protein in the culture supernatant. It can be used in a research setting as a measure of viral burden because the number of patient cells required to produce a positive culture is indicative of cellular viral load.作者: 皮薩 時間: 2025-3-24 11:43
https://doi.org/10.1007/978-3-030-21784-6The predominant mode of transmission of HIV-1 worldwide is sexual intercourse. Therefore, there has been growing interest in studying HIV-1 in genital secretions. Given the urgency to develop a vaccine to protect against HIV-1, techniques have been developed to isolate and quantitate HIV-1 from genital secretions.作者: airborne 時間: 2025-3-24 17:12
Christina Hajisoteriou,Panayiotis AngelidesA salient feature regarding the HIV (and all the retroviruses in general) replicative cycle is the retrotranscription of the RNA genome to DNA (provirus), followed by its integration in the genome of the host cell.作者: 運動性 時間: 2025-3-24 20:15
Methods of Culturing HIV-1 from SemenThe predominant mode of transmission of HIV-1 worldwide is sexual intercourse. Therefore, there has been growing interest in studying HIV-1 in genital secretions. Given the urgency to develop a vaccine to protect against HIV-1, techniques have been developed to isolate and quantitate HIV-1 from genital secretions.作者: 一致性 時間: 2025-3-25 00:28
Quantitative PCR for HIV-1 Proviral DNAA salient feature regarding the HIV (and all the retroviruses in general) replicative cycle is the retrotranscription of the RNA genome to DNA (provirus), followed by its integration in the genome of the host cell.作者: 預(yù)感 時間: 2025-3-25 03:20 作者: Ballerina 時間: 2025-3-25 08:41
Clayton A. Hartjen,S. PriyadarsiniM HIV-Is are further divided into a growing number of subtypes (A through H at this writing). Although DNA sequence analysis is the gold standard technique for HIV-1 genetic subtyping, molecular hybridization of untyped viral sequences with subtype-specific probes is frequently used as a subtyping screen.作者: 鄙視 時間: 2025-3-25 13:53
The Globalization of Asian Cuisinestherapy (.,.). These findings, combined with the introduction of potent new antivirals (.,.), have stimulated a growing interest in viral load monitoring, both as a function of disease status, and as a predictor of disease progression and therapeutic efficacy.作者: 圓木可阻礙 時間: 2025-3-25 18:17
mpt to overcome this problem, HIV-1 has been evaluated in genital secretions using polymerase chain reaction (PCR) techniques. Compared to culture techniques, PCR techniques, both DNA and RNA, have had a higher recovery rate of HIV-1 from genital secretions (.,.,.,.–.,.–.).作者: 行業(yè) 時間: 2025-3-25 23:14
Quantitation of Cell-Free HIV by Reverse Transcriptase Activity culture supernatants. Virus is concentrated from the supernatant and separated from soluble cellular contaminants by pelleting in polyethylene glycol and resuspended in a minimal volume of suspension buffer prior to assay.作者: Observe 時間: 2025-3-26 04:04 作者: observatory 時間: 2025-3-26 04:25
Quantification of HIV-1 RNA in Dried Plasma Spots (DPS)therapy (.,.). These findings, combined with the introduction of potent new antivirals (.,.), have stimulated a growing interest in viral load monitoring, both as a function of disease status, and as a predictor of disease progression and therapeutic efficacy.作者: 陶醉 時間: 2025-3-26 11:47 作者: Mingle 時間: 2025-3-26 13:59
Carlos M. Peláez,Carlos A. Peláeze periodic addition of freshly stimulated normal donor PBMCs. During this cocultivation period, culture fluids are harvested at regular intervals and tested for the presence and subsequent replication of HIV. Cultures failing to demonstrate evidence of virus expression within 35 d are usually terminated.作者: saturated-fat 時間: 2025-3-26 19:30 作者: 有危險 時間: 2025-3-27 00:26
Timothy Scott Brown,Andrew Lisonive, requiring frequent sampling and passaging of the infected cultures. Input virus may be carried over and mistaken for virus production at early points in the growth curve. In addition, there is the biohazard associated with working with live virus.作者: Acupressure 時間: 2025-3-27 03:24 作者: 責(zé)怪 時間: 2025-3-27 08:18 作者: Banister 時間: 2025-3-27 11:11 作者: 一大群 時間: 2025-3-27 14:51
IV RNA (.,.), steady-state transcriptional levels and splicing patterns of HIV RNA as a result of antiviral constructs (.), and receptor studies (.,.). In addition, Northern blotting has be utilized to answer questions on the effect on the HIV RNA level of expression of the envelope protein on calmodulin (.) and carbohydrate binding proteins (.).作者: Moderate 時間: 2025-3-27 19:07 作者: 抑制 時間: 2025-3-28 00:52 作者: flamboyant 時間: 2025-3-28 04:09 作者: 內(nèi)行 時間: 2025-3-28 07:19 作者: 來自于 時間: 2025-3-28 10:35 作者: entrance 時間: 2025-3-28 16:27
The Globalisation of Higher Educationn of disease (.,.) as well. Now accepted as a surrogate for all of these features of HIV-1 disease, specific guidelines have been adopted for the use of viral load measures in the clinical management of the disease (.).作者: defuse 時間: 2025-3-28 19:34 作者: 假設(shè) 時間: 2025-3-29 01:57
Quantitation of HIV-1, DNA and RNA From Single Frozen Cell Pelletsn of disease (.,.) as well. Now accepted as a surrogate for all of these features of HIV-1 disease, specific guidelines have been adopted for the use of viral load measures in the clinical management of the disease (.).作者: scrutiny 時間: 2025-3-29 04:13 作者: 使?jié)M足 時間: 2025-3-29 09:10 作者: FAZE 時間: 2025-3-29 11:51
culture assay based on the detection of HIV in patient peripheral blood mononuclear cells (PBMC) by the appearance of p24 gag protein in the culture supernatant. It can be used in a research setting as a measure of viral burden because the number of patient cells required to produce a positive cult作者: 詼諧 時間: 2025-3-29 17:06
The Global Rise of Asian Transformatione detection of reverse transcriptase activity (RT) indicates the presence of HIV-1 in in vitro cultures of human peripheral blood mononuclear cells (PBMCs). Because active HIV-1 infection results in lysis of lymphocytes and release of free virions, the associated enzyme activity can be found in cell作者: Affiliation 時間: 2025-3-29 23:45
marily replicates in CD4. T-lymphocytes, and such viruses are termed clinical or primary isolates. As early as 1986, the in vitro replicative capacity and cell tropism of primary HIV-1 isolates were shown to be important in the pathophysiology of HIV-1 infection (.). High replication capacity in PBM作者: Minatory 時間: 2025-3-30 00:07
https://doi.org/10.1007/978-3-030-57473-4CD4. cells. This coreceptor was first identified by Berger and colleagues who showed that fusion and entry of T-cell line-adapted strains of HIV-1 into CD4. cells were mediated by a member of the seven transmembrane chemokine receptor family (.). This protein was initially termed “fusin” and later f作者: 連鎖 時間: 2025-3-30 05:34
Timothy Scott Brown,Andrew Lison low multiplicity of infection (MOI) and washed extensively to remove input virus. Samples of culture supernatant are then removed over approximately a two week period and frozen. Virus in the supernatants is then quantitated by reverse transcriptase assay or by ELISA for p24. antigen. Using live vi作者: alcoholism 時間: 2025-3-30 10:54
The Global Thermohaline Paleocirculationto cells. For HIV-1 to infect cells, the virus must bind to the cell surface, after which the viral Env protein must be triggered to undergo a conformational change that mediates membrane fusion. Cell-surface binding has long been known to occur via a high-affinity interaction between Env and CD4. H作者: hauteur 時間: 2025-3-30 15:27
Clayton A. Hartjen,S. Priyadarsini correlate with disease state (.–.). The genetic diversity of the viral swarm within an HIV-1 infected individual is so extensive that this entity has been termed as quasi-species. Geographic distributions of HIV-1 reveal sequence clustering into a major group M and a minor (outlier) group O. Group 作者: 驕傲 時間: 2025-3-30 19:53
RNA is first separated by size through a denaturing agarose gel and transferred onto a membrane. With this transfer or blotting, and subsequent hybridization with a DNA or RNA probe, quantitation, expression levels of the RNA, size of the RNA, and mapping of the 5′- and 3′-terminal end of the RNA c作者: drusen 時間: 2025-3-31 00:16 作者: 男學(xué)院 時間: 2025-3-31 02:06
https://doi.org/10.1007/978-3-319-91995-9loned proviruses. Only a handful of full-length clones exist (.), and of these, even a smaller fraction are infectious upon transfection into cell lines. Furthermore, the available infectious clones only represent subtypes B and D (.). The main reasons for the lack of infectious clones are related t作者: Annotate 時間: 2025-3-31 08:56 作者: Adenocarcinoma 時間: 2025-3-31 13:14 作者: avarice 時間: 2025-3-31 14:35 作者: employor 時間: 2025-3-31 18:39 作者: Truculent 時間: 2025-3-31 23:30 作者: 形容詞詞尾 時間: 2025-4-1 04:54
https://doi.org/10.1007/978-3-642-59246-1regulatory sequences (.–.). The LTR from a prototypical, laboratory-adapted HIV-1 (HXB2) can be divided into modulatory, core promoter, and transactivating regions (.). The modulatory regions include elements with limited homology to AP-1 enhancer sequences at positions ?347 to ?329 relative to the 作者: 珍奇 時間: 2025-4-1 06:12 作者: 光滑 時間: 2025-4-1 10:21 作者: Presbyopia 時間: 2025-4-1 17:05
