標(biāo)題: Titlebook: HIV Protocols; Vinayaka R. Prasad,Ganjam V. Kalpana Book 2016Latest edition Springer Science+Business Media New York 2016 HIV-1 RNA struct [打印本頁(yè)] 作者: 法庭 時(shí)間: 2025-3-21 19:05
書(shū)目名稱HIV Protocols影響因子(影響力)
作者: 深陷 時(shí)間: 2025-3-21 20:50 作者: Tincture 時(shí)間: 2025-3-22 01:38 作者: 贊美者 時(shí)間: 2025-3-22 08:38 作者: 羊欄 時(shí)間: 2025-3-22 11:20 作者: apropos 時(shí)間: 2025-3-22 16:53
https://doi.org/10.1057/9781137291301ng the emergence of drug resistance, immune system escape, and invasion of distinct anatomical compartments. Extensive research has focused on understanding how various cellular and viral factors alter the rates and types of mutations produced during viral replication. Here, we describe a single-cyc作者: 刻苦讀書(shū) 時(shí)間: 2025-3-22 19:57 作者: indigenous 時(shí)間: 2025-3-22 22:55
https://doi.org/10.1057/9780230511330ely been applied in virology. Because it has an RNA genome, RNA-protein interactions play critical roles in human immunodeficiency virus type 1 (HIV-1) replication. In many cases, the binding sites of proteins on HIV-1 RNA molecules in physiologically relevant settings are not known. .ross-.inking-.作者: maculated 時(shí)間: 2025-3-23 01:32 作者: Hallowed 時(shí)間: 2025-3-23 06:05 作者: 仔細(xì)閱讀 時(shí)間: 2025-3-23 13:27 作者: 侵略者 時(shí)間: 2025-3-23 14:24 作者: 小口啜飲 時(shí)間: 2025-3-23 21:37 作者: resilience 時(shí)間: 2025-3-24 01:07 作者: ARCH 時(shí)間: 2025-3-24 02:39 作者: 深陷 時(shí)間: 2025-3-24 08:25 作者: fidelity 時(shí)間: 2025-3-24 12:56
Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometryd an uninfected, CD4-expressing “target” cell. In vitro studies indicate that HIV-1 cell-to-cell infection is much more efficient than infection by cell-free viral particles; however, the exact mechanisms of the enhanced efficiency of this infection pathway are still unclear. Several assays have bee作者: 江湖騙子 時(shí)間: 2025-3-24 18:07
HIV-1 Capsid Stabilization Assayfects in HIV-1 reverse transcription and infection. We developed a novel and simple assay to measure stability of in vitro-assembled HIV-1 CA-NC complexes. This assay allowed us to demonstrate that cytosolic extracts strongly stabilize the HIV-1 core (Fricke et al., J Virol 87:10587–10597, 2013). By作者: 原告 時(shí)間: 2025-3-24 20:12
Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Vi of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.作者: antipsychotic 時(shí)間: 2025-3-25 03:10
HIV-1 Reverse Transcriptase-Based Assay to Determine Cellular dNTP Concentrations methods are currently employed to determine dNTP concentrations from cellular extracts. Here, we describe a highly efficient, HIV-1 reverse transcriptase (RT)-based assay to quantitate dNTP concentrations. The assay is based on the ability of HIV-1 RT to function at very low dNTP concentrations, th作者: Ascendancy 時(shí)間: 2025-3-25 05:23
Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual-Repong the emergence of drug resistance, immune system escape, and invasion of distinct anatomical compartments. Extensive research has focused on understanding how various cellular and viral factors alter the rates and types of mutations produced during viral replication. Here, we describe a single-cyc作者: 繁榮地區(qū) 時(shí)間: 2025-3-25 07:46 作者: 產(chǎn)生 時(shí)間: 2025-3-25 15:05 作者: Capture 時(shí)間: 2025-3-25 19:48
Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Protes through a progression of host RNPs composed of RNA-binding proteins regulating all stages of synthesis, processing, nuclear transport, translation, decay, and co-localization with assembling virions. RNA affinity chromatography is a versatile method to identify RNA-binding proteins to investigate 作者: Confidential 時(shí)間: 2025-3-26 00:02
Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integraseric IN inhibitors or ALLINIs, which potently impair viral replication by promoting aberrant, higher order IN multimerization as well as inhibit IN interactions with its cellular cofactor, LEDGF/p75, has opened new venues to exploit IN multimerization as a therapeutic target. Furthermore, the recent 作者: 夜晚 時(shí)間: 2025-3-26 00:46 作者: meretricious 時(shí)間: 2025-3-26 08:00
Methods to Study Determinants for Membrane Targeting of HIV-1 Gag In Vitron drives viral particle assembly at the plasma membrane via three different types of interactions: protein-protein, protein-RNA, and protein-membrane interactions. As an approach to tease apart the importance of these interactions during viral particle assembly, in particular at the step of Gag memb作者: Functional 時(shí)間: 2025-3-26 08:54 作者: 貧窮地活 時(shí)間: 2025-3-26 13:07
Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Micedying HIV-1 pathogenesis, prevention and therapies. HIV-1 infection of hu-mice results in chronic viremia and CD4+ T cell loss, thus mimicking key aspects of the disease progression. In addition, the new generation hu-mice are permissive for HIV-1 sexual transmission by vaginal and rectal routes thu作者: meritorious 時(shí)間: 2025-3-26 19:58
High-Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesisal human and mouse proteins required for HIV-1 replication. This has limited their use for in vivo assessment of anti-HIV-1 therapeutics and the mechanism by which cofactors, such as illicit drug use accelerate HIV-1 replication and disease course in substance abusers. Here, we describe the developm作者: Nonflammable 時(shí)間: 2025-3-27 00:18
Vinayaka R. Prasad,Ganjam V. KalpanaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: visual-cortex 時(shí)間: 2025-3-27 02:45 作者: Tractable 時(shí)間: 2025-3-27 09:17 作者: 終端 時(shí)間: 2025-3-27 10:19 作者: 施魔法 時(shí)間: 2025-3-27 17:35
https://doi.org/10.1057/9781137537614 of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.作者: 離開(kāi)可分裂 時(shí)間: 2025-3-27 18:34
Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Vi of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.作者: 災(zāi)禍 時(shí)間: 2025-3-28 01:30
https://doi.org/10.1007/978-1-4939-3046-3HIV-1 RNA structure; HIV-1 latency; HIV-1 replication; NeuroAIDS; RNA protein interactions; Virology作者: JOG 時(shí)間: 2025-3-28 03:58 作者: 半身雕像 時(shí)間: 2025-3-28 06:57
Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometryuorescent virus particles, can be used to measure cell-to-cell transfer of virus particles. HIV NL-GI, a clone that expresses GFP as an early gene, facilitates the measure of productive infection after cell-to-cell contact. Lastly, a variation of the β-lactamase (BlaM)-Vpr fusion assay can be used t作者: heckle 時(shí)間: 2025-3-28 11:59
Novel Biochemical Tools for Probing HIV RNA Structureres of RNA, while probing strategies that utilize “through-space” cleavage reagents such as methidiumpropyl-EDTA (MPE) and peptides appended with an ATCUN (amino terminal copper/nickel binding motif) can provide insight into 3D organization. Combinational application of these techniques provides a f作者: ASTER 時(shí)間: 2025-3-28 17:44
Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seqify the RNA molecules that are bound by the HIV-1 Gag protein in cells and in virions. This protocol can easily be applied to other viral and cellular RNA-binding proteins that influence HIV-1 replication.作者: DEMN 時(shí)間: 2025-3-28 19:08
Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrasees for the inhibitor-induced aberrant IN multimerization, a dynamic light scattering-based assay which allows for monitoring the formation and sizes of oligomeric IN particles in a time-dependent manner, and a chemical cross-linking-based assay of interacting IN subunits which allows for the determi作者: 轉(zhuǎn)折點(diǎn) 時(shí)間: 2025-3-29 01:54
High-Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesisase (HIV-LucR) and enables investigators to use bioluminescence to visualize and quantitate the temporal effects of therapeutics on HIV-1 infection. The second model, hCD4/R5/cT1 mice, consists of transgenic mice carrying human CD4, CCR5 and cyclin T1 genes, which enables murine CD4-expressing cells作者: 高貴領(lǐng)導(dǎo) 時(shí)間: 2025-3-29 06:38
https://doi.org/10.1007/978-3-642-11522-6uorescent virus particles, can be used to measure cell-to-cell transfer of virus particles. HIV NL-GI, a clone that expresses GFP as an early gene, facilitates the measure of productive infection after cell-to-cell contact. Lastly, a variation of the β-lactamase (BlaM)-Vpr fusion assay can be used t作者: 迷住 時(shí)間: 2025-3-29 08:30 作者: OFF 時(shí)間: 2025-3-29 12:54 作者: 債務(wù) 時(shí)間: 2025-3-29 17:12 作者: 鋪?zhàn)?nbsp; 時(shí)間: 2025-3-29 21:06
The Global Old Age Care Industryase (HIV-LucR) and enables investigators to use bioluminescence to visualize and quantitate the temporal effects of therapeutics on HIV-1 infection. The second model, hCD4/R5/cT1 mice, consists of transgenic mice carrying human CD4, CCR5 and cyclin T1 genes, which enables murine CD4-expressing cells作者: 偽造 時(shí)間: 2025-3-30 02:53 作者: lattice 時(shí)間: 2025-3-30 05:39
https://doi.org/10.1007/978-3-319-66215-2CA-NC complexes (Fricke et al., J Virol 87:10587–10597, 2013). Here we describe in detail the use of this capsid stability assay. We believe that our assay can be a powerful tool to assess HIV-1 capsid stability in vitro.作者: 匍匐前進(jìn) 時(shí)間: 2025-3-30 11:54 作者: alliance 時(shí)間: 2025-3-30 16:20 作者: 緩解 時(shí)間: 2025-3-30 18:13
