標(biāo)題: Titlebook: HIV Protocols ; Vinayaka R. Prasad,Ganjam V. Kalpana Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclus [打印本頁] 作者: abandon 時(shí)間: 2025-3-21 17:46
書目名稱HIV Protocols 影響因子(影響力)
書目名稱HIV Protocols 影響因子(影響力)學(xué)科排名
書目名稱HIV Protocols 網(wǎng)絡(luò)公開度
書目名稱HIV Protocols 網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱HIV Protocols 被引頻次
書目名稱HIV Protocols 被引頻次學(xué)科排名
書目名稱HIV Protocols 年度引用
書目名稱HIV Protocols 年度引用學(xué)科排名
書目名稱HIV Protocols 讀者反饋
書目名稱HIV Protocols 讀者反饋學(xué)科排名
作者: Engaged 時(shí)間: 2025-3-21 21:11
Single-Cell Single-Molecule RNA-FISH Combined with Immunofluorescence and High-Speed and High-Resolunted information about cell-to-cell variability in reactivation. Here, we describe the single-cell single-molecule RNA-FISH (smRNA-FISH) method to visualize HIV-1 gag RNA combined with the immunofluorescence (IF) method to detect Gag protein to characterize the reactivated cells. This method allows 作者: HALL 時(shí)間: 2025-3-22 03:00
Single-Virion Analysis: A Method to Visualize HIV-1 Particle Content Using Fluorescence Microscopyanalysis was first developed to study HIV-1 RNA genome packaging. In this assay, HIV-1 unspliced RNA is labeled with a fluorescently tagged RNA-binding protein (protein A) and some of the Gag proteins are labeled with a different fluorescent protein (protein B). Using fluorescence microscopy, HIV-1 作者: 感激小女 時(shí)間: 2025-3-22 06:22 作者: 含糊其辭 時(shí)間: 2025-3-22 11:19 作者: 銀版照相 時(shí)間: 2025-3-22 14:54 作者: 我不明白 時(shí)間: 2025-3-22 17:35 作者: 有限 時(shí)間: 2025-3-22 23:52 作者: 中和 時(shí)間: 2025-3-23 03:34
The Glaciers of Equatorial East Africanted information about cell-to-cell variability in reactivation. Here, we describe the single-cell single-molecule RNA-FISH (smRNA-FISH) method to visualize HIV-1 gag RNA combined with the immunofluorescence (IF) method to detect Gag protein to characterize the reactivated cells. This method allows 作者: Myocyte 時(shí)間: 2025-3-23 08:44
https://doi.org/10.1007/978-0-387-76700-0analysis was first developed to study HIV-1 RNA genome packaging. In this assay, HIV-1 unspliced RNA is labeled with a fluorescently tagged RNA-binding protein (protein A) and some of the Gag proteins are labeled with a different fluorescent protein (protein B). Using fluorescence microscopy, HIV-1 作者: Liability 時(shí)間: 2025-3-23 09:48
https://doi.org/10.1057/9780230611702the development of a robust fluorescence microscopy method to quantify the translocation of CPSF6 into nuclear speckles following HIV-1 infection. This assay holds the potential to support studies aimed at understanding the role of CPSF6 translocation to nuclear speckles in HIV-1 infection. Addition作者: Cholesterol 時(shí)間: 2025-3-23 16:57 作者: 純樸 時(shí)間: 2025-3-23 20:35 作者: 世俗 時(shí)間: 2025-3-24 01:17 作者: kindred 時(shí)間: 2025-3-24 06:01 作者: FATAL 時(shí)間: 2025-3-24 08:19 作者: Lipoprotein(A) 時(shí)間: 2025-3-24 12:16 作者: COUCH 時(shí)間: 2025-3-24 16:29
Vinayaka R. Prasad,Ganjam V. KalpanaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 不適 時(shí)間: 2025-3-24 20:08 作者: 公社 時(shí)間: 2025-3-25 02:45 作者: Dedication 時(shí)間: 2025-3-25 06:54 作者: filicide 時(shí)間: 2025-3-25 11:07
The Global Cosmopolitan Mindset, the Rev-Response Element (RRE). HIV primary isolates differ with respect to the sequence and functional activity of the Rev-RRE system. Here, we describe a high throughput assay system for analyzing Rev-RRE functional activity using packageable viral vectors.作者: 全國性 時(shí)間: 2025-3-25 12:27 作者: PURG 時(shí)間: 2025-3-25 17:40 作者: CLEFT 時(shí)間: 2025-3-25 21:19
https://doi.org/10.1007/978-3-031-48105-5describe the methods used to assess the composition and liquid–liquid phase separation (LLPS) properties of these organelles using fluorescence microscopy. The study of HIV-1 MLOs represents a new frontier that may reveal previously unknown key players in the fate of HIV-infected cells.作者: 威脅你 時(shí)間: 2025-3-26 03:33 作者: Kinetic 時(shí)間: 2025-3-26 05:36
RNA-FISH for HIV Transcription/Localization Analysiss, visualization, and the subsequent analysis of the data acquired. These parameters play a pivotal role in enhancing our comprehension of the molecular processes during infection, particularly at the crucial transcription phase of the viral life cycle.作者: 歪曲道理 時(shí)間: 2025-3-26 10:32 作者: 本能 時(shí)間: 2025-3-26 14:54
Chromatin Immunoprecipitation of Retroviral Genomes with Antibodies Recognizing Modified Histones anis chapter, a method for chromatin immunoprecipitation of unintegrated DNA is described. This technique allows investigators to examine histone and co-factor interactions with unintegrated viral DNAs as well as to analyze histone modifications in general or in a kinetic fashion at various time points during viral infection.作者: 分發(fā) 時(shí)間: 2025-3-26 17:09 作者: 的闡明 時(shí)間: 2025-3-26 22:04
Book 2024Latest editionw modifications to the viral RNA that impacts HIV biology, and new types of intracellular compartments. The chapters in this book are organized into seven parts and cover topics such as HIV latency reactivation via single molecule RNA detection; T cell responses; new and efficacious anti-HIV CAR T c作者: Parallel 時(shí)間: 2025-3-27 05:01 作者: Chauvinistic 時(shí)間: 2025-3-27 09:03
Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues imaging (MSI) and laser capture microdissection, could further expand spatial imaging capabilities into high-resolution OMIC approaches such as proteomic, lipidomics, small molecule, and drug discovery. Here, we will describe a protocol to integrate the detection of rare viral reservoirs with imaging mass spectrometry.作者: 主講人 時(shí)間: 2025-3-27 12:16
https://doi.org/10.1057/9780230510456racterization of the assembly of viral proteins at the plasma membrane of infected host cells at the single protein level. Here, we describe the TIRF equipment, the T-cell culture for HIV-1, the sample preparation for single-molecule localization microscopies such as PALM and STORM, acquisition protocols, and Gag assembling cluster analysis.作者: 商議 時(shí)間: 2025-3-27 14:59
https://doi.org/10.1007/978-3-030-13016-9. Using this technique, nuclear import of infectious particles can be monitored in both primary and cell culture models. In response to host factor depletion or restriction factors, changes in HIV-1 nuclear import can be effectively measured using the nuclear import kinetics (NIK) assay.作者: Exclaim 時(shí)間: 2025-3-27 18:36
https://doi.org/10.1057/9780230281530A from HIV-1-infected primary CD4. T-cells based on immunoprecipitation. The enriched RNA with m.A modifications can be used in a variety of downstream applications to determine the methylation status of viral or cellular RNA at resolution from transcript level down to single nucleotide.作者: entail 時(shí)間: 2025-3-28 00:10
Visualizing HIV-1 Assembly at the T-Cell Plasma Membrane Using Single-Molecule Localization Microscoracterization of the assembly of viral proteins at the plasma membrane of infected host cells at the single protein level. Here, we describe the TIRF equipment, the T-cell culture for HIV-1, the sample preparation for single-molecule localization microscopies such as PALM and STORM, acquisition protocols, and Gag assembling cluster analysis.作者: 躺下殘殺 時(shí)間: 2025-3-28 03:10 作者: 噴出 時(shí)間: 2025-3-28 09:48
Method for the Enrichment of ,-Methyladenosine-Modified Cellular and HIV-1 RNAA from HIV-1-infected primary CD4. T-cells based on immunoprecipitation. The enriched RNA with m.A modifications can be used in a variety of downstream applications to determine the methylation status of viral or cellular RNA at resolution from transcript level down to single nucleotide.作者: stroke 時(shí)間: 2025-3-28 11:37 作者: 煞費(fèi)苦心 時(shí)間: 2025-3-28 15:06
Imaging HIV-1 Nuclear Import, Uncoating, and Proviral Transcriptionlution. Very few HIV-1 particles in a viral population are infectious and successfully complete replication (~1/50). Single-particle live-cell imaging enables the study of these rare infectious viral particles, which cannot be accomplished in biochemical assays that measure the average property of t作者: 令人苦惱 時(shí)間: 2025-3-28 19:08
RNA-FISH for HIV Transcription/Localization Analysisobservation of messenger RNA (mRNA) molecules associated with particular genes of significance. This technique can also be applied to examine viral transcription and the localization of said transcripts within infected cells. In this context, we provide a comprehensive protocol for the detection, lo作者: Mortar 時(shí)間: 2025-3-29 01:32
Single-Cell Single-Molecule RNA-FISH Combined with Immunofluorescence and High-Speed and High-Resolune of the key strategies involves the reactivation of latent HIV-1 from cells using latency-reversing agents. However, currently it is unclear whether any of the latency-reversing agents are able to completely reactivate HIV-1 provirus transcription in all latent cells. An understanding of the react作者: Geyser 時(shí)間: 2025-3-29 04:31
Visualizing HIV-1 Assembly at the T-Cell Plasma Membrane Using Single-Molecule Localization Microsco visualization of nanoscaled objects in cell biology. Currently, the use of a recent generation of super-resolution fluorescence microscope coupled with improved fluorescent probes gives the possibility to study the replicative cycle of viruses in living cells, at the single-virus particle or protei作者: 縮影 時(shí)間: 2025-3-29 10:04 作者: Eosinophils 時(shí)間: 2025-3-29 12:23
Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues acquisition, software, spatial resolution, and equipment, including confocal, live-cell, super-resolution, and electron microscopy (scanning, transmission, focused ion beam, and cryo-electron microscopy). However, the recent evolution of different laser-related techniques, such as mass spectrometry作者: 貪婪地吃 時(shí)間: 2025-3-29 19:09
Characterization of Nuclear HIV-Induced Membraneless Organelles Through Fluorescence Microscopyese steps has a significant impact on HIV overall replication, including integration site selection and viral gene expression. Recently, another important phenomenon has been uncovered as part of HIV interplay with the nuclear environment, specifically involving the cleavage and polyadenylation spec作者: 商業(yè)上 時(shí)間: 2025-3-29 20:42
Detection of CPSF6 in Biomolecular Condensates as a Reporter of HIV-1 Nuclear Importeus triggers the translocation of CPSF6/CPSF5 from paraspeckles into nuclear speckles, forming puncta-like structures. While this phenomenon is well-documented, the efficiency of CPSF6 translocation to nuclear speckles upon HIV-1 infection varies depending on the type of cell used. In some human cel作者: paradigm 時(shí)間: 2025-3-30 03:26 作者: 政府 時(shí)間: 2025-3-30 07:35 作者: badinage 時(shí)間: 2025-3-30 10:43
Chromatin Immunoprecipitation of Retroviral Genomes with Antibodies Recognizing Modified Histones anviral DNAs is one such antiviral strategy and seems to be of fundamental importance, since most cell types silence unintegrated retroviral DNAs. In this chapter, a method for chromatin immunoprecipitation of unintegrated DNA is described. This technique allows investigators to examine histone and co作者: amputation 時(shí)間: 2025-3-30 13:51 作者: 方舟 時(shí)間: 2025-3-30 16:51 作者: 過于平凡 時(shí)間: 2025-3-30 23:49
Direct Analysis of HIV mRNA m6A Methylation by Nanopore Sequencingy to accurately map and quantify RNA modifications. Modification-specific antibodies or modification-sensitive endonucleases coupled with short-read RNA sequencing technologies have allowed for low-resolution or limited mapping of important regulatory modifications of HIV RNA such as N.-methyladenos作者: Aprope 時(shí)間: 2025-3-31 03:57 作者: 起來了 時(shí)間: 2025-3-31 05:46 作者: Harrowing 時(shí)間: 2025-3-31 10:46
https://doi.org/10.1007/978-3-319-77566-1observation of messenger RNA (mRNA) molecules associated with particular genes of significance. This technique can also be applied to examine viral transcription and the localization of said transcripts within infected cells. In this context, we provide a comprehensive protocol for the detection, lo作者: 字形刻痕 時(shí)間: 2025-3-31 16:17
The Glaciers of Equatorial East Africane of the key strategies involves the reactivation of latent HIV-1 from cells using latency-reversing agents. However, currently it is unclear whether any of the latency-reversing agents are able to completely reactivate HIV-1 provirus transcription in all latent cells. An understanding of the react作者: 沙草紙 時(shí)間: 2025-3-31 17:43 作者: Sleep-Paralysis 時(shí)間: 2025-3-31 23:22
https://doi.org/10.1007/978-0-387-76700-0nd viral proteins, are essential for viral replication, whereas others, such as host innate immune proteins, can inhibit virus replication. Therefore, analyzing the virion content is an integral part of studying HIV-1 replication. Traditionally, virion contents have been examined using biochemical a作者: 無底 時(shí)間: 2025-4-1 03:22
https://doi.org/10.1007/978-3-642-35500-4 acquisition, software, spatial resolution, and equipment, including confocal, live-cell, super-resolution, and electron microscopy (scanning, transmission, focused ion beam, and cryo-electron microscopy). However, the recent evolution of different laser-related techniques, such as mass spectrometry作者: A精確的 時(shí)間: 2025-4-1 08:47 作者: 殘酷的地方 時(shí)間: 2025-4-1 13:17 作者: 男學(xué)院 時(shí)間: 2025-4-1 17:54
https://doi.org/10.1007/978-3-030-13016-9clear pore complex (NPC). Current assay to measure HIV-1 nuclear import relies on a transient byproduct of HIV-1 integration failure called 2-LTR circles. However, 2-LTR circles require complete or near-complete reverse transcription and association with the non-homologous end joining (NHEJ) machine
