標(biāo)題: Titlebook: HDAC/HAT Function Assessment and Inhibitor Development; Methods and Protocol Oliver H. Kr?mer Book 2017 Springer Science+Business Media New [打印本頁] 作者: Novice 時間: 2025-3-21 17:09
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書目名稱HDAC/HAT Function Assessment and Inhibitor Development網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱HDAC/HAT Function Assessment and Inhibitor Development被引頻次
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書目名稱HDAC/HAT Function Assessment and Inhibitor Development年度引用
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書目名稱HDAC/HAT Function Assessment and Inhibitor Development讀者反饋
書目名稱HDAC/HAT Function Assessment and Inhibitor Development讀者反饋學(xué)科排名
作者: reject 時間: 2025-3-21 23:31
https://doi.org/10.1007/978-1-4020-4968-2 the response of the immune system, like the B cell function. HDACi treatment affects differentiation, proliferation, and survival of B cells. Here we describe how to investigate the effects of HDACi treatment on na?ve B cells regarding class-switch recombination (CSR) in vitro using flow cytometry.作者: 小臼 時間: 2025-3-22 03:51
https://doi.org/10.1057/978-1-137-56104-6 lines. This plasmid-based method, first published by Pierce et al. 1999 from Maria Jasin’s laboratory, is used along with flow cytometry for demonstrating the positive regulation of class I histone deacetylases on the repair of DNA double-strand breaks by homologous recombination.作者: 背心 時間: 2025-3-22 05:57
https://doi.org/10.1007/978-3-031-29746-5heir ability to target and kill cancer cells in vivo. Here, we describe the xenografting of (germ cell) cancer cell lines into the flank of nude mice and the subsequent intravenous application of HDIs.作者: 骨 時間: 2025-3-22 09:40 作者: 不可侵犯 時間: 2025-3-22 14:59 作者: BRIDE 時間: 2025-3-22 17:57
Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Ho lines. This plasmid-based method, first published by Pierce et al. 1999 from Maria Jasin’s laboratory, is used along with flow cytometry for demonstrating the positive regulation of class I histone deacetylases on the repair of DNA double-strand breaks by homologous recombination.作者: LEVER 時間: 2025-3-23 00:07 作者: Digest 時間: 2025-3-23 05:06
Oliver H. Kr?merIncludes cutting-edge methods and protocols to study HDACs and HATs.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includ作者: 浮雕 時間: 2025-3-23 06:04
Methods in Molecular Biologyhttp://image.papertrans.cn/h/image/420177.jpg作者: commune 時間: 2025-3-23 09:42 作者: refine 時間: 2025-3-23 13:54
978-1-4939-8226-4Springer Science+Business Media New York 2017作者: antidote 時間: 2025-3-23 19:54
HDAC/HAT Function Assessment and Inhibitor Development978-1-4939-6527-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Harness 時間: 2025-3-24 00:56 作者: Inelasticity 時間: 2025-3-24 05:15
https://doi.org/10.1057/9780230599857followed by selection of CD34. progenitor cells. AML-LTC cells can be maintained in liquid culture supplemented with cytokines and utilized for in vitro analyses to assess proliferation, apoptosis, expression of surface proteins or intracellular proteins and signal transduction, respectively.作者: BUST 時間: 2025-3-24 06:35
https://doi.org/10.1057/9781403918659technique, which has gained popularity recently, is the analysis of protein–protein interactions by the proximity ligation assay (PLA). Here, we describe a liver perfusion protocol and the detection of the histone deacetylase function of Sirtuin 6 (SIRT6) using PLA in pmHep.作者: Estrogen 時間: 2025-3-24 11:53 作者: CURL 時間: 2025-3-24 17:57 作者: adipose-tissue 時間: 2025-3-24 20:02
Establishment and Characterization of Long-Term Cultures Derived from Primary Acute Myeloid Leukemiafollowed by selection of CD34. progenitor cells. AML-LTC cells can be maintained in liquid culture supplemented with cytokines and utilized for in vitro analyses to assess proliferation, apoptosis, expression of surface proteins or intracellular proteins and signal transduction, respectively.作者: Modify 時間: 2025-3-25 02:41
Assessing the Histone Deacetylase Activity of SIRT6 in Primary Murine Hepatocytes via Proximity Ligatechnique, which has gained popularity recently, is the analysis of protein–protein interactions by the proximity ligation assay (PLA). Here, we describe a liver perfusion protocol and the detection of the histone deacetylase function of Sirtuin 6 (SIRT6) using PLA in pmHep.作者: 期滿 時間: 2025-3-25 03:42 作者: perpetual 時間: 2025-3-25 08:04 作者: ANN 時間: 2025-3-25 13:27 作者: MAG 時間: 2025-3-25 18:13 作者: Talkative 時間: 2025-3-25 20:15 作者: 重疊 時間: 2025-3-26 02:10
Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localizationn tumor cells, thus leading to severe mitotic defects often followed by apoptosis. In this chapter, we describe how HDACi-induced changes of the CPC localization can be analyzed by indirect immunofluorescence using CPC-specific primary and fluorophore-coupled secondary antibodies followed by confocal microscopy.作者: 執(zhí) 時間: 2025-3-26 04:37 作者: 卡死偷電 時間: 2025-3-26 10:17
https://doi.org/10.1007/978-90-481-8969-4tionated cell populations could be used for further studies. In our laboratory, these protocols are routinely used to specifically investigate the molecular basis of histone acetylation/acylation-driven epigenetic programming.作者: 催眠 時間: 2025-3-26 14:08
The Future of Orthopaedic Sports Medicinement procedure itself, the tumor growth surveillance, the explantation procedure, and finally the handling of obtained xenograft tissues are described step by step. This technical description is completed by numerous tips and alternatives designed to allow for an easy adaptation and transfer to other laboratories.作者: travail 時間: 2025-3-26 17:11 作者: enchant 時間: 2025-3-27 00:24
Generation of Xenotransplants from Human Cancer Biopsies to Assess Anti-cancer Activities of HDACiment procedure itself, the tumor growth surveillance, the explantation procedure, and finally the handling of obtained xenograft tissues are described step by step. This technical description is completed by numerous tips and alternatives designed to allow for an easy adaptation and transfer to other laboratories.作者: STENT 時間: 2025-3-27 01:35
Book 2017bleshooting and avoiding known pitfalls..Comprehensive and practical, .HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols. serves as an ideal guide to this vital area of study..作者: 使成波狀 時間: 2025-3-27 07:47
https://doi.org/10.1007/978-3-030-53544-5TUNEL staining and the caspase-3/7 activity assay. Moreover, we summarize flow cytometric analysis of propidium iodide uptake, annexin V staining, cell cycle status, ROS levels, and mitochondrial membrane potential as well as detection of apoptosis by Western blot.作者: vasculitis 時間: 2025-3-27 10:07 作者: 從容 時間: 2025-3-27 13:50
HDAC/HAT Function Assessment and Inhibitor DevelopmentMethods and Protocol作者: 胰島素 時間: 2025-3-27 18:34 作者: endoscopy 時間: 2025-3-28 00:56
https://doi.org/10.1007/978-3-030-68253-8Bonhoeffer and colleagues to examine mechanisms of axonal guidance. The design of this assay allows cells to decide between two different substrates offered at the same time. Utilizing alternating neuronal substrates for migration analyses we can partially mimic the complex in vivo situation for bra作者: 物質(zhì) 時間: 2025-3-28 02:40 作者: peak-flow 時間: 2025-3-28 10:06
https://doi.org/10.1007/978-3-8349-9764-7ng, classification, and measurement of the detected DNA fiber tracks. The obtained quantitative data can be evaluated statistically to reveal the immediate or long-term effects of DNA-damaging agents, DNA repair inhibitors, and epigenetic modulators like HDAC inhibitors on DNA replication in normal 作者: 地牢 時間: 2025-3-28 14:30 作者: AXIOM 時間: 2025-3-28 18:28
Using Histone Deacetylase Inhibitors to Analyze the Relevance of HDACs for Translationmerous disease states, including cancer, where the upregulation of HDAC family members leads to dysregulation of genes and proteins involved in cell proliferation, cell cycle regulation, and apoptosis. These observations have pushed HDAC inhibitors (HDACi) to the forefront of therapeutic development作者: ensemble 時間: 2025-3-28 19:56 作者: hypnotic 時間: 2025-3-29 02:33
https://doi.org/10.1007/978-3-319-26896-5and the response to chemotherapy. Here we describe how autophagy can be monitored in living cells by flow cytometry using the cationic amphiphilic tracer dye Cyto-ID. Green. The detection of autophagy induction in the human leukemia cell line K562 after the treatment with the HDAC class I inhibitor 作者: 認(rèn)識 時間: 2025-3-29 06:36 作者: 銀版照相 時間: 2025-3-29 08:43
https://doi.org/10.1007/978-3-030-53544-5lation and methylation, regulate their structure and control gene expression. Histone acetyltransferases (HATs) acetylate lysine residues in histones while histone deacetylases (HDACs) remove this modification. HDAC inhibitors (HDACi) can alter gene expression patterns and induce cytotoxicity in can作者: avulsion 時間: 2025-3-29 13:18 作者: frugal 時間: 2025-3-29 18:02
https://doi.org/10.1007/978-3-030-68253-8nisms. In diseases such as cancer deregulated cell migration represents a major problem. Therefore, numerous efforts are under way to understand the molecular mechanisms of tumor cell migration and to generate more efficient tumor therapies. Cell migration assays are one of the most commonly used fu作者: meritorious 時間: 2025-3-29 23:15
The Future of Liberal Democracycetylases (HDAC). HAT activity results in the addition of acetyl groups on the lysine residues of histone tails leading to decondensation of the chromatin, and increased gene transcription in general, whereas HDACs remove these acetyl groups, thus leading to an overall suppression of gene transcript作者: 吞下 時間: 2025-3-30 01:48 作者: lavish 時間: 2025-3-30 04:13
https://doi.org/10.1007/978-3-8349-9764-7 provides information on the DNA replication progress and its regulation under normal conditions as well as on replication stress induced by environmental genotoxic agents or cancer drugs. The method relies on the detection of incorporated thymidine analogues during DNA synthesis in the S phase of t作者: 聯(lián)合 時間: 2025-3-30 11:32
https://doi.org/10.1057/978-1-137-56104-6 lines. This plasmid-based method, first published by Pierce et al. 1999 from Maria Jasin’s laboratory, is used along with flow cytometry for demonstrating the positive regulation of class I histone deacetylases on the repair of DNA double-strand breaks by homologous recombination.作者: 完整 時間: 2025-3-30 14:20 作者: 出血 時間: 2025-3-30 18:55 作者: 很是迷惑 時間: 2025-3-30 21:20
https://doi.org/10.1007/978-90-481-8969-4l transitions during spermatogenesis. Large-scale cell purification techniques allow for combined proteomics, genomics, and transcriptomics studies. Herein, we describe a procedure for the purification of meiotic and post-meiotic male germ cells from adult mouse testes. We also describe how the frac作者: 精致 時間: 2025-3-31 02:19 作者: OWL 時間: 2025-3-31 08:14
