標(biāo)題: Titlebook: HDAC/HAT Function Assessment and Inhibitor Development; Methods and Protocol Oliver H. Kr?mer Book 2023Latest edition The Editor(s) (if app [打印本頁] 作者: osteomalacia 時(shí)間: 2025-3-21 19:12
書目名稱HDAC/HAT Function Assessment and Inhibitor Development影響因子(影響力)
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書目名稱HDAC/HAT Function Assessment and Inhibitor Development讀者反饋
書目名稱HDAC/HAT Function Assessment and Inhibitor Development讀者反饋學(xué)科排名
作者: guardianship 時(shí)間: 2025-3-21 21:41 作者: Amplify 時(shí)間: 2025-3-22 02:16
Evaluation of Antitumor and On-Target Activity of HDAC Inhibitors with the Zebrafish Embryo Xenografctivity of HDAC inhibitors was verified through immunohistochemistry staining on paraffin-embedded early larvae. Overall, the zebrafish embryo xenotransplantation model allows for fast and cost-efficient in vivo evaluation of targeted drug toxicity, efficacy, and on-target activity in the field of p作者: 天然熱噴泉 時(shí)間: 2025-3-22 05:49 作者: Hamper 時(shí)間: 2025-3-22 12:47 作者: geometrician 時(shí)間: 2025-3-22 13:11 作者: Aura231 時(shí)間: 2025-3-22 19:58 作者: mechanical 時(shí)間: 2025-3-22 22:39 作者: Latency 時(shí)間: 2025-3-23 02:54 作者: Outspoken 時(shí)間: 2025-3-23 08:17
Marco J. Mariotto,Gordon L. Paulr-Blue to determine HDACi-induced cell death in healthy/diseased cellular models. We next focus on assessing the target engagement of inhibitors with the appropriate HDAC isoforms in a cellular environment via Western blotting of acetylated HDAC substrates. Finally, we provide detailed guidelines on作者: 摸索 時(shí)間: 2025-3-23 10:08 作者: Aqueous-Humor 時(shí)間: 2025-3-23 16:41 作者: Libido 時(shí)間: 2025-3-23 18:25
1064-3745 expertsThis fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for作者: Ostrich 時(shí)間: 2025-3-23 22:14 作者: 縮短 時(shí)間: 2025-3-24 06:12 作者: inundate 時(shí)間: 2025-3-24 08:14
The Christian Right in American Politics, regulation of gene expression. We here describe the generation of a genetic toolbox by the CRISPR/Cas9 methodology in nearly haploid human tumor cells. This novel model system allows to discriminate between catalytic and structural functions of class I HDAC enzymes and to mimic the treatment with specific HDAC inhibitors.作者: Yourself 時(shí)間: 2025-3-24 10:39 作者: 大暴雨 時(shí)間: 2025-3-24 18:28 作者: Nuance 時(shí)間: 2025-3-24 21:27
Human Platelet Lysate as Valid Cell Growth Additive to Assess Protein Acetylationtail?how to generate and use hPL as a cost-effective substitute for FCS in experiments with mammalian cell cultures. A large panel of cells and conditions can be cultured and tested in media with hPL.作者: Coordinate 時(shí)間: 2025-3-25 02:42 作者: Observe 時(shí)間: 2025-3-25 05:08
Fenianism Subdued and Authority Upheld?,tors on DNA damage and cell viability. Here, we report an efficient and fast protocol for the isolation of mpH by liver perfusion. These mpH can be used for downstream applications such as the detection of the DNA damage marker γH2AX by confocal laser scanning microscopy.作者: CAMP 時(shí)間: 2025-3-25 07:46 作者: ostensible 時(shí)間: 2025-3-25 13:50
Analyzing Lymphoma Development and Progression Using HDACi in Mouse Modelsfore, the development of specific HDAC inhibitors may extend the therapeutic strategy for cancer therapy. Here, we describe how to investigate the therapeutic potential of specific HDACi by treatment in a mouse model for B-cell lymphoma, exemplified by the HDAC6 inhibitor Marbostat-100.作者: 委屈 時(shí)間: 2025-3-25 18:27
Colony Formation Assay to Test the Impact of HDACi on Leukemic Cellsmonitored in vitro and can provide insights into the sensitivity of tumor cells to chemotherapeutics. The following chapter describes how clonogenic hematopoietic cell growth can be determined with the colony formation assay.作者: conjunctivitis 時(shí)間: 2025-3-25 20:43 作者: 抗原 時(shí)間: 2025-3-26 00:24
The Christopher Columbus Encyclopediaient and transgenic mice provide a useful tool to understand the mechanisms of aging and related pathologies. In this chapter, we summarized the most widely applied methods to understand the physiopathological function of SIRT7 in mice.作者: lymphoma 時(shí)間: 2025-3-26 04:34
The Chronic Crisis of American Democracyar drug release and activity. Suitable methods, opportunities, and challenges will be discussed to provide general guidelines for the analysis of HDACi drug carrier systems with a special focus on recently developed cellulose-based VPA-coupled NPs.作者: Brocas-Area 時(shí)間: 2025-3-26 09:40 作者: 他日關(guān)稅重重 時(shí)間: 2025-3-26 15:00 作者: Factorable 時(shí)間: 2025-3-26 19:56 作者: sperse 時(shí)間: 2025-3-26 22:25
Investigating Physiopathological Roles for Sirtuins in a Mouse Modelient and transgenic mice provide a useful tool to understand the mechanisms of aging and related pathologies. In this chapter, we summarized the most widely applied methods to understand the physiopathological function of SIRT7 in mice.作者: MELON 時(shí)間: 2025-3-27 02:28 作者: Extort 時(shí)間: 2025-3-27 08:12 作者: 使人入神 時(shí)間: 2025-3-27 12:55
HDACi Delivery Systems Based on Cellulose Valproate Nanoparticlestion. The esterification of three hydroxy groups of cellulose with VPA leads to products having a high amount of VPA loading. Subsequent shaping yielded uniform nanoparticles (NPs) of around 150?nm in size capable of releasing VPA in a controlled way under physiological conditions.作者: BUCK 時(shí)間: 2025-3-27 14:29 作者: 墊子 時(shí)間: 2025-3-27 20:04 作者: FIG 時(shí)間: 2025-3-27 22:45
Colony Formation Assay to Test the Impact of HDACi on Leukemic Cellsiving therapy can be resistant to the treatment, but they can also lose the ability to proliferate. The ability of single cells to proliferate can be monitored in vitro and can provide insights into the sensitivity of tumor cells to chemotherapeutics. The following chapter describes how clonogenic h作者: Crater 時(shí)間: 2025-3-28 02:44 作者: Rotator-Cuff 時(shí)間: 2025-3-28 09:29 作者: Gratulate 時(shí)間: 2025-3-28 14:20 作者: 使害羞 時(shí)間: 2025-3-28 14:35 作者: armistice 時(shí)間: 2025-3-28 22:27
Investigating Physiopathological Roles for Sirtuins in a Mouse Model metabolism, DNA repair, epigenetics, gene expression, cell proliferation, differentiation, and survival. Using genetically modified model organisms, sirtuins are proved to be one of the most conserved aging-regulatory and longevity-promoting genes/pathways among species. Of the seven sirtuins, SIRT作者: 混合物 時(shí)間: 2025-3-29 01:21 作者: VOC 時(shí)間: 2025-3-29 04:59 作者: Debark 時(shí)間: 2025-3-29 09:32
Development of Pyrazine-Anilinobenzamides as Histone Deacetylase HDAC1–3 Selective Inhibitors and Bithe synthesis of a novel series of class-I selective HDAC inhibitors containing anilinobenzamide moieties as ZBG connected with a central (piperazin-1-yl)pyrazine moiety. Compounds were tested in vitro against class-I HDAC1, 2, and 3 isoforms. Some highly potent HDAC inhibitors were obtained and wer作者: Vulnerable 時(shí)間: 2025-3-29 12:04
Evaluation of Small-Molecule HDAC Inhibitors Through In Vitro and In Cellulo Approachesl-molecule inhibitors that target one or more members of the HDAC protein family. Emerging HDAC inhibitors that show promise in drug discovery programs must be assessed across a range of . assays to establish an inhibitor profile for potency and cellular selectivity towards target HDAC(s) as well as作者: Rustproof 時(shí)間: 2025-3-29 19:17
Synthesis, Biochemical, and Cellular Evaluation of HDAC6 Targeting Proteolysis Targeting Chimeras functions and in the diseased state. Proteolysis-targeting chimeras (PROTACs) are bifunctional molecules that hijack the cell’s ubiquitin-proteasome system (UPS). One of the promising targets for this approach is histone deacetylase 6 (HDAC6), which is highly expressed in several types of cancers a作者: abstemious 時(shí)間: 2025-3-29 22:45 作者: 結(jié)果 時(shí)間: 2025-3-30 00:36
Synthesis and Characterization of Reversible Covalent HDAC4 Inhibitorsreasing the residence time and prolonging the inhibitory effect on the target protein under nonequilibrium conditions. Herein, we describe the synthetic access to cyanoacrylate-based HDAC4 inhibitors and the procedures for the characterization of the transient nature of the covalent bond between cya作者: configuration 時(shí)間: 2025-3-30 08:06 作者: meritorious 時(shí)間: 2025-3-30 09:21 作者: 浮雕 時(shí)間: 2025-3-30 14:17 作者: consolidate 時(shí)間: 2025-3-30 19:17
The German Model: Secular Dominanceiving therapy can be resistant to the treatment, but they can also lose the ability to proliferate. The ability of single cells to proliferate can be monitored in vitro and can provide insights into the sensitivity of tumor cells to chemotherapeutics. The following chapter describes how clonogenic h作者: 暫時(shí)別動 時(shí)間: 2025-3-30 22:55
The Christian Philosophy of William Templearmacological inhibitors that specifically target oncoproteins and their executing protein complex partners. In acute myeloid leukemia (AML), transcription factors such as RUNX1 and MLL1, which are important for normal blood cell development, frequently harbor mutations including chromosomal translo作者: 貝雷帽 時(shí)間: 2025-3-31 03:53
The Christian Right in American Politics,g targets for the treatment of cancer, neurological, and immunological disorders. These enzymes have both catalytic and non-catalytic functions in the regulation of gene expression. We here describe the generation of a genetic toolbox by the CRISPR/Cas9 methodology in nearly haploid human tumor cell作者: 搜尋 時(shí)間: 2025-3-31 05:01
https://doi.org/10.1007/978-3-030-30089-0for xenotransplantation studies. Here, we describe the evaluation of toxicity, efficacy, and on-target activity of histone deacetylase (HDAC) inhibitors in a zebrafish embryo yolk sac xenotransplantation model of medulloblastoma and neuroblastoma cells. For this, we performed toxicity assays with ou
