作者: Project 時間: 2025-3-21 20:41 作者: 卜聞 時間: 2025-3-22 03:33 作者: 谷物 時間: 2025-3-22 08:35 作者: 進步 時間: 2025-3-22 10:09
Ton J. Cleophas,Aeilko H. Zwindermanhogenesis, where decreased TM cell numbers and alterations to the amount and composition of extracellular matrix hinder outflow of aqueous humor fluid from the anterior chamber. This causes intraocular pressure (IOP) elevation. Elevated IOP, a main risk factor for primary open-angle glaucoma, damage作者: MIR 時間: 2025-3-22 16:26 作者: MIR 時間: 2025-3-22 20:18 作者: delegate 時間: 2025-3-22 23:22 作者: lethal 時間: 2025-3-23 04:20 作者: 小歌劇 時間: 2025-3-23 09:34 作者: 高興去去 時間: 2025-3-23 09:51
Correlation: From Relationshipsat produces a homogeneous intraocular pressure (IOP) response and can be induced in any mouse strain of choice without the need for extensive breeding. The procedure utilizes an ocular hypertension and glaucoma-relevant transgene, bioactivated TGFβ2, and an adenovirus 5 virus with select tropism for作者: 巨碩 時間: 2025-3-23 14:17
https://doi.org/10.1007/978-1-4614-5743-5ical and pathological features of primary open-angle glaucoma (POAG), and therefore mouse models of GC-induced glaucoma are utilized to study pathophysiology of glaucoma. We have recently demonstrated that weekly periocular injections of dexamethasone-21-acetate (Dex-Ac) lead to robust and significa作者: 含鐵 時間: 2025-3-23 19:33 作者: inscribe 時間: 2025-3-24 00:45 作者: gerontocracy 時間: 2025-3-24 06:13 作者: BARGE 時間: 2025-3-24 08:22
Statistics for Business and Economicsfor the early detection of glaucoma but for the elucidation of potential mechanisms underlying retinal ganglion cell loss. Early reports of the selective vulnerability of larger retinal ganglion cells (RGCs) in human studies did not translate simply to the loss of a particular class of RGC but more 作者: 多產(chǎn)魚 時間: 2025-3-24 13:29
Statistics for Business and Economics. During the process of ganglion cell injury, morphological changes can occur prior to gross structural changes and cell death. Similarly, following injury, functional changes can occur in the absence of substantive structural changes. These more subtle effects can often be detected using functional作者: Harpoon 時間: 2025-3-24 17:02
Probability Concepts and Their Analysise both the scotopic and photopic visions of nonrestrained animals through tracking head movement, providing a quantitative estimate of visual functions. In restrained animals, optokinetic response (OKR), compensatory eye movements for visual shifts in the surroundings, is utilized. Both OMR and OKR 作者: 摘要記錄 時間: 2025-3-24 20:17
https://doi.org/10.1007/978-3-319-21509-9tic nerve damage for decades. Methods that produce sustained IOP elevation can mimic the chronic nature of glaucoma and produce optic nerve damage. However, the pressure course for individual animals can be variable, unpredictably high at times, and difficult to monitor with current tonometry method作者: reptile 時間: 2025-3-25 01:56
Evaluation of Cross-Linked Actin Networks (CLANs) in Human Trabecular Meshwork Cells and Tissues,oss and blindness. An overall increase in resistance to aqueous humor outflow causes sustained elevation in IOP. Glaucomatous insults in the aqueous humor outflow pathway, including the trabecular meshwork (TM), precede such chronic physiological changes in IOP. These insults include ultrastructural作者: 完整 時間: 2025-3-25 03:28
Three-Dimensional Extracellular Matrix Protein Hydrogels for Human Trabecular Meshwork Cell Studiesnt a realistic three-dimensional extracellular milieu to cells in vitro. Here, we describe the fabrication and use of soft tissue-mimetic extracellular matrix protein hydrogels for investigations of human trabecular meshwork cell biology.作者: 內(nèi)疚 時間: 2025-3-25 09:23 作者: 含鐵 時間: 2025-3-25 13:56
Measurements of Phagocytosis in Trabecular Meshwork Cells,Among the functions of the trabecular meshwork that help to prevent this restriction and maintain the movement of aqueous humor is phagocytosis. Cells in the trabecular meshwork actively phagocytose pigment, cellular debris, and other materials in order to help keep the drainage pathway clear. A dec作者: Breach 時間: 2025-3-25 16:54
Nanotubules and Cellular Communication in Trabecular Meshwork Cells,hogenesis, where decreased TM cell numbers and alterations to the amount and composition of extracellular matrix hinder outflow of aqueous humor fluid from the anterior chamber. This causes intraocular pressure (IOP) elevation. Elevated IOP, a main risk factor for primary open-angle glaucoma, damage作者: 聲明 時間: 2025-3-25 20:31 作者: laparoscopy 時間: 2025-3-26 02:51
Time-Lapse Live-Cell Imaging Using Fluorescent Protein Sensors in Outflow Pathway Cells Under FluidSC) of the eye is an emerging field. Shear stress has been shown to activate mechanosensitive ion channels in TM cells and induce nitric oxide production in SC cells, which can affect outflow resistance and lower IOP. Live-cell imaging using fluorescent protein sensors has provided real-time data to作者: 盡管 時間: 2025-3-26 05:53
Human Anterior Segment Perfusion Organ Culture,essure regulation. In this model, anterior segments dissected from human donor eyes can be fixed to a modified petri dish and perfused with media along with various study agents at the physiological flow rate of 2.5?μL/min. The model mimics the one-way flow of aqueous humor in human eyes and can be 作者: AORTA 時間: 2025-3-26 11:44 作者: 擔憂 時間: 2025-3-26 16:17
Human Corneal Rim Perfusion Culture,es which are expensive. Here, we describe a method using human corneal rims as an alternative to anterior segments for perfusion culture. In this method, the human corneal rim is glued to a three-dimensional-printed perfusion plates instead of using mechanical compression in traditional perfusion cu作者: 殘暴 時間: 2025-3-26 17:46
,The Inducible TGFβ2 Ocular Hypertension Mouse Model,at produces a homogeneous intraocular pressure (IOP) response and can be induced in any mouse strain of choice without the need for extensive breeding. The procedure utilizes an ocular hypertension and glaucoma-relevant transgene, bioactivated TGFβ2, and an adenovirus 5 virus with select tropism for作者: theta-waves 時間: 2025-3-26 22:36
Mouse Model of Glucocorticoid-Induced Glaucoma,ical and pathological features of primary open-angle glaucoma (POAG), and therefore mouse models of GC-induced glaucoma are utilized to study pathophysiology of glaucoma. We have recently demonstrated that weekly periocular injections of dexamethasone-21-acetate (Dex-Ac) lead to robust and significa作者: 完成才會征服 時間: 2025-3-27 03:58 作者: LASH 時間: 2025-3-27 09:18
Aqueous Humor Angiography, humor outflow pathways. As a translational technique, it is applicable for in vivo imaging in living subjects and ex vivo imaging using whole-globe preparations or anterior segment perfusion setups. Excellent spatial and temporal resolution has enabled insights into the segmental distribution of aq作者: Felicitous 時間: 2025-3-27 10:33 作者: filial 時間: 2025-3-27 14:12 作者: 索賠 時間: 2025-3-27 17:49 作者: fluffy 時間: 2025-3-28 01:10
Testing Visual Function by Assessment of the Optomotor Reflex in Glaucoma,e both the scotopic and photopic visions of nonrestrained animals through tracking head movement, providing a quantitative estimate of visual functions. In restrained animals, optokinetic response (OKR), compensatory eye movements for visual shifts in the surroundings, is utilized. Both OMR and OKR 作者: STALE 時間: 2025-3-28 02:37
Controlled Elevation of Intraocular Pressure (CEI) Glaucoma Model in Rats,tic nerve damage for decades. Methods that produce sustained IOP elevation can mimic the chronic nature of glaucoma and produce optic nerve damage. However, the pressure course for individual animals can be variable, unpredictably high at times, and difficult to monitor with current tonometry method作者: Pseudoephedrine 時間: 2025-3-28 06:29
Evaluation of Cross-Linked Actin Networks (CLANs) in Human Trabecular Meshwork Cells and Tissues,ads to formation of polygonal arrays within the cytoplasm, resembling a geodesic dome-like structure, that we identified as CLANs. In addition to increased amounts of CLANs in POAG TM cells and tissues, we also discovered that glucocorticoid (GC) and TGFβ2 signaling pathways associated with the deve作者: Instrumental 時間: 2025-3-28 10:28 作者: insincerity 時間: 2025-3-28 16:25
Mouse Model of Glucocorticoid-Induced Glaucoma,g excessive extracellular matrix deposition and induction of endoplasmic reticulum stress. Mouse model of Dex-induced glaucoma represents an ideal animal model to investigate both glaucomatous damage to the TM and RGC axons and to develop novel therapies. Here, we present a detailed protocol for dev作者: Extricate 時間: 2025-3-28 20:53
Retinal Ganglion Cell Transplantation: Differentiation and Quantification Strategies,comes involving confocal imaging of retinal flat-mounts and custom ImageJ and MATLAB scripts to map and analyze the spatial distribution of donor RGCs within the host retina. Altogether, this approach provides a robust framework for exploring RGC transplantation as a potential therapy for vision los作者: GRIN 時間: 2025-3-29 00:45 作者: 虛弱的神經(jīng) 時間: 2025-3-29 05:48
Testing Visual Function by Assessment of the Optomotor Reflex in Glaucoma,ty (CS), the gold standards for measuring clinical vision, they provide reliable and easily accessible results that further eye and brain research. These methods of sight evaluation have been used in multiple animal models, particularly mice and zebrafish. Through OMR assays, these animal models hav作者: 現(xiàn)任者 時間: 2025-3-29 10:50 作者: aptitude 時間: 2025-3-29 12:11
https://doi.org/10.1007/978-3-642-25859-6ads to formation of polygonal arrays within the cytoplasm, resembling a geodesic dome-like structure, that we identified as CLANs. In addition to increased amounts of CLANs in POAG TM cells and tissues, we also discovered that glucocorticoid (GC) and TGFβ2 signaling pathways associated with the deve作者: 疾馳 時間: 2025-3-29 17:39 作者: 突襲 時間: 2025-3-29 21:00
https://doi.org/10.1007/978-1-4614-5743-5g excessive extracellular matrix deposition and induction of endoplasmic reticulum stress. Mouse model of Dex-induced glaucoma represents an ideal animal model to investigate both glaucomatous damage to the TM and RGC axons and to develop novel therapies. Here, we present a detailed protocol for dev作者: 兇兆 時間: 2025-3-30 02:37
D. J. Finney CBE, ScD, FRS, FRSEcomes involving confocal imaging of retinal flat-mounts and custom ImageJ and MATLAB scripts to map and analyze the spatial distribution of donor RGCs within the host retina. Altogether, this approach provides a robust framework for exploring RGC transplantation as a potential therapy for vision los作者: certitude 時間: 2025-3-30 06:41 作者: moratorium 時間: 2025-3-30 09:30 作者: maladorit 時間: 2025-3-30 15:33 作者: circumvent 時間: 2025-3-30 18:59 作者: Gobble 時間: 2025-3-30 22:29
The Interpretation of The P-Values, the size and concentration of exosome samples. Transmission electron microscopy can directly show the size and morphology of exosomes. Moreover, the sample should be characterized by the expression of several exosome biomarker proteins. Exosomal contents such as proteins and miRNAs could be profiled using appropriate technologies.作者: deciduous 時間: 2025-3-31 02:57 作者: SYN 時間: 2025-3-31 08:00 作者: occurrence 時間: 2025-3-31 09:55 作者: 書法 時間: 2025-3-31 16:11
Measurements of Phagocytosis in Trabecular Meshwork Cells,rease in phagocytosis has thus been implicated in the development of glaucomas. In this chapter, we outline two methods using fluorescent markers that can be used to measure the phagocytic properties of trabecular meshwork cells in culture.作者: ALB 時間: 2025-3-31 19:12
Isolation and Characterization of Exosomes from Ocular Samples, the size and concentration of exosome samples. Transmission electron microscopy can directly show the size and morphology of exosomes. Moreover, the sample should be characterized by the expression of several exosome biomarker proteins. Exosomal contents such as proteins and miRNAs could be profiled using appropriate technologies.作者: 有偏見 時間: 2025-3-31 22:02
Human Anterior Segment Perfusion Organ Culture,used to evaluate the effects of various drugs on eye pressure in real time. Using this model, cells and tissues of the anterior segment can be maintained for up to 28?days, enabling histological and molecular evaluations.作者: Cardioversion 時間: 2025-4-1 01:56
Human Corneal Rim Perfusion Culture,lture setup. The corneal rim and perfusion plate are placed onto a special holder for perfusion culture. This method is affordable and easy to set up. The instruments for traditional perfusion culture studies can be used for this model without modification.作者: LINE 時間: 2025-4-1 06:23
