書目名稱Group A Streptococcus網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Group A Streptococcus被引頻次
書目名稱Group A Streptococcus被引頻次學(xué)科排名
書目名稱Group A Streptococcus年度引用
書目名稱Group A Streptococcus年度引用學(xué)科排名
書目名稱Group A Streptococcus讀者反饋
書目名稱Group A Streptococcus讀者反饋學(xué)科排名
作者: 無底 時間: 2025-3-21 21:01
https://doi.org/10.1007/978-3-030-39407-3presented approach can be used for the analysis of all subcellular proteomes from . and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membrane are of particular interest for the development of new antimicrobial therapies.作者: Strength 時間: 2025-3-22 01:47
Protocol for Proteome Analysis of Group A Streptococcuspresented approach can be used for the analysis of all subcellular proteomes from . and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membrane are of particular interest for the development of new antimicrobial therapies.作者: chronicle 時間: 2025-3-22 04:44
https://doi.org/10.1057/9780230276086iplex PCR reactions to detect genes encoding 20 virulence factors: .3, ., .B, .D, .B, .CEP, .A, ., sic, .L, .K, .M, .C, .I, .A, .H, .G, .J, .Z, and .. Classification of strains based on the virulence factors absence or presence correlates with PFGE MLST and emm typing results. The typing/detection s作者: prediabetes 時間: 2025-3-22 08:58 作者: 平庸的人或物 時間: 2025-3-22 15:52
Hysterical Hypnosis and Infectious Theatre,siology. The Tn-seq approach allows the . monitoring of highly complex mutant libraries, leveraging massive parallel DNA sequencing as a means to characterize the composition of these mutant pools on a genome-scale with unprecedented nucleotide-level high resolution. In this chapter, we present step作者: 平庸的人或物 時間: 2025-3-22 20:02
Performing Objects and Theatrical Thingsdifferent strains. Here, we outline an optimized, rapid method for creating markerless isogenic mutations that combines Gibson assembly cloning with a new temperature-sensitive plasmid, pLZts. This method is highly efficient and reduces the time needed to create GAS mutants to ~2–3?weeks, with the a作者: jabber 時間: 2025-3-22 22:14
https://doi.org/10.1007/978-3-319-96701-1ethod for labeling GAS with a set of plasmids we have developed and deposited with Addgene. These plasmids can be used to either integrate firefly luciferase (Ffluc) or red-shifted firefly luciferase (FflucRT) into the GAS genome or to introduce the reporters on plasmids which have been stabilized w作者: conifer 時間: 2025-3-23 05:18
https://doi.org/10.1007/978-981-10-7998-6nical advancements and the development of new bioinformatic tools for analyzing genomic data; however, the basic principles and processes for defining and processing high-quality genome sequence information remain unchanged. Here, we introduce some considerations and describe some commonly used bioi作者: Cupidity 時間: 2025-3-23 06:47
Michael Lambert,Tamantha Hammerschlagies, real-time PCR or microarray studies were the mainstay of assessing differences in gene expression in bacteria. Real-time PCR remains a critical tool for targeted gene expression analyses. However, microarray studies have given way to the plethora of advantages in RNA sequencing (RNA-seq) for th作者: 中止 時間: 2025-3-23 12:07
https://doi.org/10.1007/978-3-030-39407-3presented approach can be used for the analysis of all subcellular proteomes from . and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membrane are of particular interest for the development of new antimicrobial therapies.作者: 可憎 時間: 2025-3-23 14:25
Performing Statelessness in Europef host glycans by pathogenic lectins is an important process that allows the adherence of bacteria to the host epithelial surface in many species, including Group A Streptococcus (GAS). Glycan microarrays present a sensitive, high-throughput approach for identifying novel lectin–glycan interactions 作者: 晚間 時間: 2025-3-23 20:30
Kim Beauchesne,Alessandra Santos virulence factors. The redundancy between many surface proteins such as adhesins also adds complexity and difficulty. . is a non-pathogenic Gram-positive species related to GAS that can be an ideal surrogate organism to circumvent this problem. Genetic manipulation in . is easy, and the mechanisms 作者: STALE 時間: 2025-3-23 23:29 作者: LATE 時間: 2025-3-24 05:21 作者: 發(fā)起 時間: 2025-3-24 09:45 作者: 裝飾 時間: 2025-3-24 13:08 作者: 鉆孔 時間: 2025-3-24 17:01 作者: 空氣 時間: 2025-3-24 21:46 作者: 狼群 時間: 2025-3-24 23:37 作者: photophobia 時間: 2025-3-25 06:09
Protocols for Tn-seq Analyses in the Group A Streptococcussiology. The Tn-seq approach allows the . monitoring of highly complex mutant libraries, leveraging massive parallel DNA sequencing as a means to characterize the composition of these mutant pools on a genome-scale with unprecedented nucleotide-level high resolution. In this chapter, we present step作者: Paraplegia 時間: 2025-3-25 08:06 作者: Insensate 時間: 2025-3-25 12:37
Generation of Bioluminescent Group A Streptococcus for Biophotonic Imagingethod for labeling GAS with a set of plasmids we have developed and deposited with Addgene. These plasmids can be used to either integrate firefly luciferase (Ffluc) or red-shifted firefly luciferase (FflucRT) into the GAS genome or to introduce the reporters on plasmids which have been stabilized w作者: 兇殘 時間: 2025-3-25 18:29 作者: 耐寒 時間: 2025-3-25 21:40 作者: inflame 時間: 2025-3-26 03:02
Protocol for Proteome Analysis of Group A Streptococcuspresented approach can be used for the analysis of all subcellular proteomes from . and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membrane are of particular interest for the development of new antimicrobial therapies.作者: esthetician 時間: 2025-3-26 07:37 作者: critic 時間: 2025-3-26 09:54
Using , as Surrogate Organism to Study Group A Streptococcus Surface Proteins virulence factors. The redundancy between many surface proteins such as adhesins also adds complexity and difficulty. . is a non-pathogenic Gram-positive species related to GAS that can be an ideal surrogate organism to circumvent this problem. Genetic manipulation in . is easy, and the mechanisms 作者: Tdd526 時間: 2025-3-26 14:26
Expression and Purification of Collagen-Like Proteins of Group A Streptococcusis often fused to additional ligand-binding domains and plays both structural and functional roles in modular “bacterial collagens.” Here, we describe the step-by-step expression and purification of the recombinant streptococcal collagen-like proteins, rScl, using the .-tag II system. The integrity 作者: 大約冬季 時間: 2025-3-26 19:23
Detection of Fibronectin-Binding Proteins of , Using Ligand Blot Analysis abundant ECM proteins and targeted by a wide variety of secreted Fn-binding proteins (Fbps) of .. However, prior to detailed kinetic analysis of that binding process, evaluations of the ability of . strains to bind to Fn as well as interactions of target molecules with Fn are required. In this chap作者: 公理 時間: 2025-3-27 00:55
Morphology and Ultrastructure of Group A Streptococcus Biofilmsunded by a bluish film are seen under a light microscope after alcian blue staining of preparations grown on coverslips. The extracellular matrix (indicator of biofilm maturity) becomes visible on ultrathin sections in transmission electron microscopy after additional staining with alcian blue; fila作者: pericardium 時間: 2025-3-27 01:57 作者: 憤怒歷史 時間: 2025-3-27 05:39 作者: 鍵琴 時間: 2025-3-27 11:27 作者: profligate 時間: 2025-3-27 16:57 作者: Urologist 時間: 2025-3-27 21:25 作者: dapper 時間: 2025-3-27 22:36 作者: NAV 時間: 2025-3-28 05:23
https://doi.org/10.1007/978-981-10-7998-6 and processing high-quality genome sequence information remain unchanged. Here, we introduce some considerations and describe some commonly used bioinformatic steps for processing raw genome sequence data to generate genome assemblies through to understanding basic population genomics.作者: 使顯得不重要 時間: 2025-3-28 09:00
Performing Statelessness in Europeluding Group A Streptococcus (GAS). Glycan microarrays present a sensitive, high-throughput approach for identifying novel lectin–glycan interactions and can be applied in the context of whole bacteria or purified bacterial proteins.作者: APO 時間: 2025-3-28 12:37 作者: MOTTO 時間: 2025-3-28 14:52
Performing the Body in Irish Theatreicator of biofilm maturity) becomes visible on ultrathin sections in transmission electron microscopy after additional staining with alcian blue; filamentous structures, characteristic of biofilm, are observed in intercellular spaces. The data obtained by scanning electron microscopy also demonstrate the presence of biofilm.作者: 全部 時間: 2025-3-28 20:29
Performing the Nation in Global Koreaents that take place in this GAS–macrophage battleground, the cellular consequences for the pathogen and for the immune cell, and the balance between the magnitude of infection and the efficiency of the host immune response can be investigated with a variety of assays presented in this chapter.作者: 花爭吵 時間: 2025-3-28 23:23 作者: critique 時間: 2025-3-29 03:13
Performing the Nation in Interwar Germanyserved lysosome-mediated catabolic process, which is critical for cellular homeostasis. Autophagy also acts as an intracellular immune system. In this section, we describe how to identify GcAVs in infected cells using fluorescent microscopy.作者: 欄桿 時間: 2025-3-29 09:06
Detection of Fibronectin-Binding Proteins of , Using Ligand Blot Analysister, we present routine procedures for ligand blot analysis with labeled human Fn, using bacterial cell wall extracts prepared by either enzymatic digestion of cells or extraction with a denaturing agent.作者: Isolate 時間: 2025-3-29 13:55
Identification of Group A Streptococcus-Containing Autophagosome-Like Vacuolesserved lysosome-mediated catabolic process, which is critical for cellular homeostasis. Autophagy also acts as an intracellular immune system. In this section, we describe how to identify GcAVs in infected cells using fluorescent microscopy.作者: BRAND 時間: 2025-3-29 17:25 作者: Fibrillation 時間: 2025-3-29 21:49 作者: 物質(zhì) 時間: 2025-3-30 03:06 作者: 終止 時間: 2025-3-30 07:37 作者: 起來了 時間: 2025-3-30 10:57
Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughpuluding Group A Streptococcus (GAS). Glycan microarrays present a sensitive, high-throughput approach for identifying novel lectin–glycan interactions and can be applied in the context of whole bacteria or purified bacterial proteins.作者: Creatinine-Test 時間: 2025-3-30 13:05
Expression and Purification of Collagen-Like Proteins of Group A Streptococcus the step-by-step expression and purification of the recombinant streptococcal collagen-like proteins, rScl, using the .-tag II system. The integrity and structural characterization of recombinant collagen-like proteins is very important for defining their function.作者: 嘲弄 時間: 2025-3-30 18:46 作者: 慷慨援助 時間: 2025-3-30 23:53
Dynamic Interactions of Group A Streptococcus with Host Macrophagesents that take place in this GAS–macrophage battleground, the cellular consequences for the pathogen and for the immune cell, and the balance between the magnitude of infection and the efficiency of the host immune response can be investigated with a variety of assays presented in this chapter.作者: 在前面 時間: 2025-3-31 04:03
Kim Beauchesne,Alessandra Santosroteins from other bacterial species, and modern molecular cloning tools and protocols have been developed. This chapter describes the workflow of generating recombinant . strains expressing GAS surface proteins and the validation and quantification of their surface expression.作者: 符合規(guī)定 時間: 2025-3-31 08:24
Using , as Surrogate Organism to Study Group A Streptococcus Surface Proteinsroteins from other bacterial species, and modern molecular cloning tools and protocols have been developed. This chapter describes the workflow of generating recombinant . strains expressing GAS surface proteins and the validation and quantification of their surface expression.作者: 觀點 時間: 2025-3-31 10:20
Hysterical Hypnosis and Infectious Theatre,seq analyses. Most of the protocols presented here were developed and implemented using the . M1T1 serotype clinical isolate 5448, but they have been successfully applied to multiple GAS serotypes as well as other pathogenic Streptococci.作者: AND 時間: 2025-3-31 14:51
Michael Lambert,Tamantha Hammerschlagns grown in vitro in standard laboratory media, including cell growth, RNA extraction, ribosomal RNA depletion, and library construction. Considerations for library sequencing and data analysis are also provided.作者: 愉快么 時間: 2025-3-31 21:06
