標(biāo)題: Titlebook: Golgi; Methods and Protocol Yanzhuang Wang,Vladimir V Lupashin,Todd R. Graham Book 2023 The Editor(s) (if applicable) and The Author(s), un [打印本頁(yè)] 作者: 照相機(jī) 時(shí)間: 2025-3-21 16:47
書目名稱Golgi影響因子(影響力)
書目名稱Golgi影響因子(影響力)學(xué)科排名
書目名稱Golgi網(wǎng)絡(luò)公開(kāi)度
書目名稱Golgi網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書目名稱Golgi被引頻次
書目名稱Golgi被引頻次學(xué)科排名
書目名稱Golgi年度引用
書目名稱Golgi年度引用學(xué)科排名
書目名稱Golgi讀者反饋
書目名稱Golgi讀者反饋學(xué)科排名
作者: 修飾 時(shí)間: 2025-3-21 21:12
Book 2023ubleshooting and avoiding known pitfalls..Cutting-edge and authoritative, .Golgi: Methods and Protocols. is a valuable tool for researchers in the field who wish to explore new areas of Golgi biology and for new investigators interested in exploring Golgi structure and function..作者: mastopexy 時(shí)間: 2025-3-22 00:38 作者: Brain-Imaging 時(shí)間: 2025-3-22 05:07 作者: 希望 時(shí)間: 2025-3-22 12:21 作者: 或者發(fā)神韻 時(shí)間: 2025-3-22 15:11 作者: 或者發(fā)神韻 時(shí)間: 2025-3-22 19:23
Monitoring Effects of Membrane Traffic Via Changes in Cell Polarity and Morphogenesis in Three-Dimenrovides unique opportunities to examine the role of membrane traffic during epithelial morphogenesis. We also present methods to process hPSC-cysts for immunofluorescence and staining with commonly used fluorescence labels useful for detecting defects in polarization and morphogenesis caused by defe作者: arrogant 時(shí)間: 2025-3-22 21:50 作者: Allodynia 時(shí)間: 2025-3-23 01:24 作者: 小步舞 時(shí)間: 2025-3-23 08:30
An Electron Tomographic Analysis of Giantin-Deficient Golgi Proposes a New Function of the Golgin Prt there exists a correlation between Golgi structural changes caused by the loss of Giantin and Golgi function. Here, we describe electron tomography methods for the detection of structural changes in the Golgi.作者: giggle 時(shí)間: 2025-3-23 11:13 作者: cartilage 時(shí)間: 2025-3-23 16:28
Quantification of Golgi Protein Mislocalization to the Budding Yeast Vacuolend retrograde vesicular trafficking. Disruptions in these signals or in the retrograde pathways often lead to mislocalization of Golgi proteins to the vacuole in budding yeast. The extent of vacuolar mislocalization can be quantified through colocalization of GFP-tagged Golgi proteins with fluoresce作者: reject 時(shí)間: 2025-3-23 19:43 作者: 睨視 時(shí)間: 2025-3-23 22:40
Microscopy and Immunocytochemistry-Based Methods to Study Cell Wall Biosynthetic Enzymes in the Golgcellulosic cell wall. The Golgi apparatus houses numerous cell wall-synthesizing or cell wall-modifying enzymes to generate the complex cell wall structure. However, several putative cell wall biosynthetic candidates await characterization, which requires verification of the subcellular localization作者: 善辯 時(shí)間: 2025-3-24 05:14 作者: 等級(jí)的上升 時(shí)間: 2025-3-24 09:15 作者: 惰性氣體 時(shí)間: 2025-3-24 13:36 作者: follicle 時(shí)間: 2025-3-24 18:23
Studying the Organization of the Golgi by Super-Resolution Microscopyibution of Golgi proteins to understand Golgi functions. The .-to-. or axial localization of a Golgi protein can be obtained using our previously developed method, Golgi protein localization by imaging centers of mass (GLIM), in nocodazole-induced Golgi ministacks (hereafter referred to as ministack作者: 火車車輪 時(shí)間: 2025-3-24 21:55 作者: 你敢命令 時(shí)間: 2025-3-24 23:22
Studying Golgi Structure and Function by Thin Section TEMM) and for 3,3′-diaminobenzidine (DAB) cytochemical staining and pre-embedding immunolabelling to localize specific Golgi proteins. Furthermore, we demonstrate how the Golgi morphology can be elucidated by classifying the Golgi membranes using Microscopy Image Browser—a software that provides anonym作者: MIRTH 時(shí)間: 2025-3-25 06:44
Algorithm for Modern Electron Microscopic Examination of the Golgi Complexsident proteins is not trivial. Fast development of microscopic methods generates a huge difficulty for Golgi researchers to select the best protocol to use. Modern methods of light microscopy, such as super-resolution light microscopy (SRLM) and electron microscopy (EM), open new possibilities in a作者: 表否定 時(shí)間: 2025-3-25 10:58
High-Pressure Freezing Followed by Freeze Substitution: An Optimal Electron Microscope Technique to t technique that freezes the samples at high pressure (~2100?bar) and low temperature (?196?°C) within milliseconds. This ultrarapid fixation enables simultaneous immobilization of all cellular components and preserves the samples in a near-native state. This facilitates the study of dynamic process作者: 國(guó)家明智 時(shí)間: 2025-3-25 12:39 作者: 大包裹 時(shí)間: 2025-3-25 18:45 作者: glacial 時(shí)間: 2025-3-25 21:45
Modeling the Cryo-EM Structure of the Exocyst Complexes from the donor compartments to the membrane of receptor compartments. The exocyst complex is an octameric MTC that tethers the post-Golgi secretory vesicles to the plasma membrane. To learn the function and regulation of the exocyst complex, it is crucial to understand the structure of the comple作者: AUGUR 時(shí)間: 2025-3-26 03:40
Quantitatively Assessing Co-Localization of Golgi Proteins by Distance Analysis Using the DiAna Softcalization assays and is based on measuring distances between pairs of objects representative of the two proteins. It makes use of a relatively recently developed ImageJ plugin called DiAna, which employs semi-automated object recognition and subsequent distance analysis of the recognized objects. T作者: Restenosis 時(shí)間: 2025-3-26 04:57 作者: GRE 時(shí)間: 2025-3-26 10:00 作者: WAIL 時(shí)間: 2025-3-26 14:30
E. Beckmann,I. Broser,R. Broserociated constituents. Here, we describe an antibody staining method to detect Golgi-associated proteins in . larval salivary glands, using the .-Golgi protein Lava lamp and the clathrin adaptor AP-1 as a suitable example. Golgi bodies immunostained using this protocol can be visualized using confoca作者: oxidize 時(shí)間: 2025-3-26 19:37
Luminescent Processes in Semiconductorscellulosic cell wall. The Golgi apparatus houses numerous cell wall-synthesizing or cell wall-modifying enzymes to generate the complex cell wall structure. However, several putative cell wall biosynthetic candidates await characterization, which requires verification of the subcellular localization作者: Estimable 時(shí)間: 2025-3-26 22:59 作者: Harridan 時(shí)間: 2025-3-27 02:06 作者: 蘆筍 時(shí)間: 2025-3-27 08:43
Crater-Hopping: Observing the Moon on Day 6,ed, and then structures are labelled using primary antibodies directly conjugated to fluorophores, or, more commonly, first with an antibody against an antigen of interest followed by a secondary antibody conjugated to a fluorophore that binds to the primary antibody. Fluorescence can be visualized 作者: kyphoplasty 時(shí)間: 2025-3-27 12:41 作者: 情愛(ài) 時(shí)間: 2025-3-27 14:07
https://doi.org/10.1007/978-88-470-2637-7or simultaneous imaging. Using this technology, we are now able to observe the fine details of various dynamic events going on in living cells, such as membrane traffic and organelle dynamics. The retention using selective hooks (RUSH) system is a powerful tool to control synchronous release of natu作者: MAIM 時(shí)間: 2025-3-27 18:47 作者: 牙齒 時(shí)間: 2025-3-27 23:00
https://doi.org/10.1007/978-1-84628-154-9sident proteins is not trivial. Fast development of microscopic methods generates a huge difficulty for Golgi researchers to select the best protocol to use. Modern methods of light microscopy, such as super-resolution light microscopy (SRLM) and electron microscopy (EM), open new possibilities in a作者: 橡子 時(shí)間: 2025-3-28 02:59 作者: CRACY 時(shí)間: 2025-3-28 07:17 作者: 失眠癥 時(shí)間: 2025-3-28 13:43 作者: Meditative 時(shí)間: 2025-3-28 15:47 作者: 反話 時(shí)間: 2025-3-28 20:45 作者: 值得尊敬 時(shí)間: 2025-3-28 23:14
Yanzhuang Wang,Vladimir V Lupashin,Todd R. GrahamIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 人類 時(shí)間: 2025-3-29 03:17
978-1-0716-2641-2The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: asthma 時(shí)間: 2025-3-29 10:37
Golgi978-1-0716-2639-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: incarcerate 時(shí)間: 2025-3-29 13:20 作者: 河流 時(shí)間: 2025-3-29 18:15 作者: commute 時(shí)間: 2025-3-29 22:55 作者: Mast-Cell 時(shí)間: 2025-3-30 02:20
Soft Lunar Landing Guidance Sensor,M) and for 3,3′-diaminobenzidine (DAB) cytochemical staining and pre-embedding immunolabelling to localize specific Golgi proteins. Furthermore, we demonstrate how the Golgi morphology can be elucidated by classifying the Golgi membranes using Microscopy Image Browser—a software that provides anonymization, modelling, and annotation.作者: 租約 時(shí)間: 2025-3-30 07:14
Live Cell Imaging of Yeast Golgi Dynamicsthrin-coated vesicle dynamics in a single focal plane at the .-Golgi network of the yeast .. This method can be readily adapted for live cell imaging of a?diverse?set of dynamic processes within cells.作者: Blazon 時(shí)間: 2025-3-30 09:47 作者: glamor 時(shí)間: 2025-3-30 15:38
Studying Golgi Structure and Function by Thin Section TEMM) and for 3,3′-diaminobenzidine (DAB) cytochemical staining and pre-embedding immunolabelling to localize specific Golgi proteins. Furthermore, we demonstrate how the Golgi morphology can be elucidated by classifying the Golgi membranes using Microscopy Image Browser—a software that provides anonymization, modelling, and annotation.作者: 無(wú)思維能力 時(shí)間: 2025-3-30 17:19 作者: 粗野 時(shí)間: 2025-3-30 21:20
Reconstitution of Golgi Biogenesis in Permeabilized , CellsThe protozoan parasite, ., offers a simple system to study the growth and duplication of the Golgi. Cell lines stably expressing a photoactivatable GFP attached to an endogenous Golgi protein are permeabilized using digitonin. Photoactivation followed by imaging can then be used to follow the formation of the new Golgi.作者: nerve-sparing 時(shí)間: 2025-3-31 02:21 作者: archaeology 時(shí)間: 2025-3-31 07:18 作者: 壓艙物 時(shí)間: 2025-3-31 10:06 作者: Pageant 時(shí)間: 2025-3-31 14:44
Quantification of Golgi Protein Mislocalization to the Budding Yeast Vacuoleuch larger vacuole is problematic for thresholding the images prior to calculating the MCC. In this chapter, we describe the use of Multi-Otsu thresholding in ImageJ to quantify the degree of GFP-tagged protein mislocalization to the vacuole. Furthermore, these methods can be applied to other colocalization events within the cell.作者: Redundant 時(shí)間: 2025-3-31 18:37
Generation of GM130 Conditional Knockout Mouses established through gene targeting, stem cell screening, and blastocyst injection. Such model has been successfully applied for studies of physiological functions of GM130 and Golgi apparatus at the cellular and animal levels.作者: Resistance 時(shí)間: 2025-4-1 00:07
Studying the Organization of the Golgi by Super-Resolution MicroscopyHere, we summarize our method to identify en face and side views of ministacks. It takes advantage of the characteristic ring and double-punctum staining patterns exhibited by cisternal rim-localized proteins. After averaging multiple en face views, the resulting image reveals the intrinsic organization of cisternae in a non-biased manner.作者: Chauvinistic 時(shí)間: 2025-4-1 02:09
Luminescent Processes in Semiconductors stably produced in transgenic plants, by confocal microscopy using fluorescent-tagged proteins along with known Golgi markers or the trafficking inhibitor brefeldin A. We also detail a procedure to analyze the enzymatic products through antibody-based immunoblotting after cell wall enrichment.作者: 敬禮 時(shí)間: 2025-4-1 09:55
https://doi.org/10.1007/978-88-470-1353-7subunits using chemical cross-linking mass spectrometry. Here, we describe the method of modeling and validating the cryo-EM structure of the exocyst complex. This method could provide a guide for modeling of other protein complexes of which the structures are solved at medium to near-atomic resolution.作者: 幸福愉悅感 時(shí)間: 2025-4-1 11:22
Pathologic Evaluation of Lung Cancer,eliant on arbitrarily set intensity threshold values. We present here a use case for the DiAna plugin in the context of plant cells with fluorescently labeled subcellular structures, such as proteins associated with the plant Golgi apparatus.作者: 帶來(lái)墨水 時(shí)間: 2025-4-1 14:48
Microscopy and Immunocytochemistry-Based Methods to Study Cell Wall Biosynthetic Enzymes in the Golg stably produced in transgenic plants, by confocal microscopy using fluorescent-tagged proteins along with known Golgi markers or the trafficking inhibitor brefeldin A. We also detail a procedure to analyze the enzymatic products through antibody-based immunoblotting after cell wall enrichment.
