標(biāo)題: Titlebook: Glycovirology; Methods and Protocol Yasuo Suzuki Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri [打印本頁] 作者: 閃爍 時(shí)間: 2025-3-21 16:57
書目名稱Glycovirology影響因子(影響力)
書目名稱Glycovirology影響因子(影響力)學(xué)科排名
書目名稱Glycovirology網(wǎng)絡(luò)公開度
書目名稱Glycovirology網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Glycovirology被引頻次
書目名稱Glycovirology被引頻次學(xué)科排名
書目名稱Glycovirology年度引用
書目名稱Glycovirology年度引用學(xué)科排名
書目名稱Glycovirology讀者反饋
書目名稱Glycovirology讀者反饋學(xué)科排名
作者: 敵意 時(shí)間: 2025-3-21 23:23 作者: prostate-gland 時(shí)間: 2025-3-22 03:32 作者: 制造 時(shí)間: 2025-3-22 07:42 作者: 確保 時(shí)間: 2025-3-22 09:38
Preparation of Glycan Arrays Using Glycopeptides Derived From Biomaterials, only because commercially available, structure-defined glycans are limited, but also because such interactions, if any, between viruses and isolated glycans are relatively weak, and thus, difficult to detect by conventional methods, e.g., enzyme-linked immune-sorbent assay. We describe a practical 作者: 宣傳 時(shí)間: 2025-3-22 13:58
Glycan Profiling of Viral Glycoproteins with the Lectin Microarray,at glycoform of the viral glycoproteins is closely related to their immunogenicity as vaccine antigens. Although mass spectrometric approach is a gold standard for the glycoproteomic analysis of viral glycoproteins, lectin microarray (LMA) is regarded as an alternative method for analyzing glycan at作者: 宣傳 時(shí)間: 2025-3-22 18:29
Preparation of Recombinant PA Endonuclease Domain Protein of Influenza A Virus and Its Application influenza A virus drugs such as glycoconjugates. To test this activity, purified viral PA endonuclease domain protein is necessary. Here, we describe a method for the expression and purification of recombinant influenza A virus PA endonuclease domain protein, and a PA endonuclease assay to test glyc作者: 陰險(xiǎn) 時(shí)間: 2025-3-22 23:10
Receptor-Binding Specificity of Influenza Viruses,hment is mediated by the viral surface glycoprotein hemagglutinin (HA). Influenza A viruses have a wide host range, meaning they are able to infect many mammal and bird species. Influenza pandemics have been caused by viruses that contain genes from avian influenza viruses. Therefore, the infection 作者: bisphosphonate 時(shí)間: 2025-3-23 03:24
Functional Analysis of Sulfatide in Influenza A Virus Infection and Replication,tract, trachea, kidney, and central nervous system. Influenza A virus binds not only to glycoconjugates terminally containing sialic acid as a viral binding receptor but also to sulfatide not containing sialic acid. On the surface of infected cells, the envelope glycoprotein hemagglutinin of influen作者: 保全 時(shí)間: 2025-3-23 09:07
Quantification of Receptor Association, Dissociation, and NA-Dependent Motility of Influenza A Partinylated synthetic sialoglycans or sialoglycoproteins (biotinylated or Fc-tagged) were immobilized on the tip of biosensors (coated with streptavidin or protein A) that were subsequently dipped into IAV particle solutions in 96-well plates. Association and/or dissociation of IAV particles was record作者: 討好女人 時(shí)間: 2025-3-23 13:31
Receptor-Binding Assay for Avian Influenza Viruses,itiate their invasion to host cells. Unlike well-known theories in human and avian influenza viruses, barriers laying between interspecies transmission of influenza viruses among bird species are not well understood. Recently, it was speculated that glycan binding of the HA to fucosylated Siaα2–3Gal作者: frugal 時(shí)間: 2025-3-23 17:51
Characterization of Influenza Virus Binding to Receptors on Isolated Cell Membranes,and mammals determine viral host range, tissue tropism, and pathogenicity. Here, we describe method that allows to characterize binding of IVs to biologically relevant cellular receptors using a conventional solid-phase enzyme-linked assay. In this method, we isolate plasma membranes from respirator作者: companion 時(shí)間: 2025-3-23 18:09 作者: CLEAR 時(shí)間: 2025-3-24 02:08
Solid-Phase Binding Assay for Ganglioside Binding of Human Respiroviruses,ter by utilizing glycoproteins or glycosphingolipids (gangliosides) containing sialic acid on the cell membrane. We developed a solid-phase binding assay to evaluate hPIV-1, hPIV-3, and Sendai virus’ abilities to bind to different types of gangliosides. hPIV1 and hPIV3 show strong binding to neolact作者: GLIDE 時(shí)間: 2025-3-24 05:26 作者: Gastric 時(shí)間: 2025-3-24 08:56 作者: seroma 時(shí)間: 2025-3-24 10:46
Roles of Sialyl Glycans in HCoV-OC43, HCoV-HKU1, MERS-CoV and SARS-CoV-2 Infections,ng pandemic SARS-CoV-2 (COVID-19) with high mutability giving some variants causing severe illness and death have been reported to attach to sialyl receptors via their spike (S) glycoproteins and via additional short spikes, hemagglutinin-esterase (HE) glycoproteins, for HCoV-OC43 and HCoV-HKU1. The作者: Eructation 時(shí)間: 2025-3-24 18:23 作者: LIKEN 時(shí)間: 2025-3-24 20:12
Assays for Determining the Sialidase Activity of Influenza Viruses and Monitoring Influenza Virus Sviruses. The fluorescent assay is cost-effective and applicable for many laboratories and is, therefore, commonly used for global monitoring of the NA inhibitor susceptibility of influenza viruses. Here, I describe, in detail, protocols for the fluorescence-based NA activity assay and the NA inhibit作者: 蕁麻 時(shí)間: 2025-3-25 02:01 作者: 永久 時(shí)間: 2025-3-25 05:57
Nikolaus Knoepffler,Nikolai Münchportant in the characterization of viruses. In this regard, the high-performance liquid chromatography (HPLC) is a widely used analytical technique. We developed a multidimensional HPLC mapping method that identifies glycan structures. This is achieved by separating and purifying glycans using three作者: 躺下殘殺 時(shí)間: 2025-3-25 10:24 作者: deviate 時(shí)間: 2025-3-25 12:59
Festigkeit von Leim- und Klebfugenu5Ac and N-glycolylneuraminic acid lie at the terminal end of sugar chains on the cell surface. Human influenza viruses preferentially bind to sialic acids bound to galactose by the alpha2-6 linkage (Neu5Acα2-6Gal), abundant in the human airway. In contrast, avian influenza viruses preferentially bi作者: JOG 時(shí)間: 2025-3-25 18:47 作者: brother 時(shí)間: 2025-3-25 20:15
Leipziger mathematische Antrittsvorlesungenat glycoform of the viral glycoproteins is closely related to their immunogenicity as vaccine antigens. Although mass spectrometric approach is a gold standard for the glycoproteomic analysis of viral glycoproteins, lectin microarray (LMA) is regarded as an alternative method for analyzing glycan at作者: 別炫耀 時(shí)間: 2025-3-26 02:28 作者: Ccu106 時(shí)間: 2025-3-26 04:40
Leistungsbewertung als p?dagogische Praktikhment is mediated by the viral surface glycoprotein hemagglutinin (HA). Influenza A viruses have a wide host range, meaning they are able to infect many mammal and bird species. Influenza pandemics have been caused by viruses that contain genes from avian influenza viruses. Therefore, the infection 作者: palette 時(shí)間: 2025-3-26 12:00 作者: 教育學(xué) 時(shí)間: 2025-3-26 15:55 作者: 神化怪物 時(shí)間: 2025-3-26 18:44
Berufsausbildung und Arbeitsmarktausgleich,itiate their invasion to host cells. Unlike well-known theories in human and avian influenza viruses, barriers laying between interspecies transmission of influenza viruses among bird species are not well understood. Recently, it was speculated that glycan binding of the HA to fucosylated Siaα2–3Gal作者: Abutment 時(shí)間: 2025-3-26 23:43
,Traditionelle Ans?tze: haben Grenzen!,and mammals determine viral host range, tissue tropism, and pathogenicity. Here, we describe method that allows to characterize binding of IVs to biologically relevant cellular receptors using a conventional solid-phase enzyme-linked assay. In this method, we isolate plasma membranes from respirator作者: Mangle 時(shí)間: 2025-3-27 03:12 作者: expunge 時(shí)間: 2025-3-27 06:57
https://doi.org/10.1007/978-3-322-97140-1ter by utilizing glycoproteins or glycosphingolipids (gangliosides) containing sialic acid on the cell membrane. We developed a solid-phase binding assay to evaluate hPIV-1, hPIV-3, and Sendai virus’ abilities to bind to different types of gangliosides. hPIV1 and hPIV3 show strong binding to neolact作者: 供過于求 時(shí)間: 2025-3-27 10:33
https://doi.org/10.1007/978-3-322-91508-5r to influenza C virus (ICV), IDV also has seven segments in its genome and has only one major surface glycoprotein, called the hemagglutinin-esterase-fusion (HEF) protein, for receptor-binding, receptor-destroying, and membrane fusion. HEF utilizes 9-O-acetylated sialic acids as its receptor and ha作者: 暗語 時(shí)間: 2025-3-27 17:16 作者: 善于騙人 時(shí)間: 2025-3-27 20:38 作者: 討好女人 時(shí)間: 2025-3-28 00:48 作者: Ferritin 時(shí)間: 2025-3-28 04:20 作者: 財(cái)產(chǎn) 時(shí)間: 2025-3-28 07:57
Leistungsbewertung als p?dagogische Praktikessary for the efficient transmission of avian influenza viruses among humans. Accordingly, constant monitoring of the receptor-binding specificity of avian influenza viruses is important. In this chapter, we describe the protocol for assessing the receptor-binding specificity of influenza A viruses作者: muffler 時(shí)間: 2025-3-28 13:29 作者: Psychogenic 時(shí)間: 2025-3-28 18:03 作者: malapropism 時(shí)間: 2025-3-28 20:53 作者: sebaceous-gland 時(shí)間: 2025-3-29 00:33 作者: 態(tài)學(xué) 時(shí)間: 2025-3-29 06:17
Nikolaus Knoepffler,Nikolai Münchar, and tissue levels and can be used to study the glycans of virological samples such as virus and their hosts. It also enables the discrimination of isomers, which is difficult via mass spectrometry. In this paper, we describe in detail the principle and method of the structural analysis of .-type glycans using the proposed method.作者: EXULT 時(shí)間: 2025-3-29 10:46
Reservoirs of Visceral Leishmaniasisomain protein was amplified with PCR and cloned into an expression vector. Six His-tagged PA endonuclease domain proteins were expressed in . and purified with Ni.-agarose resin and imidazole using an ion-exchange column. Using the recombinant PA endonuclease domain protein, an endonuclease assay was performed.作者: Insul島 時(shí)間: 2025-3-29 15:23 作者: 征稅 時(shí)間: 2025-3-29 16:35 作者: Chemotherapy 時(shí)間: 2025-3-29 19:59
https://doi.org/10.1007/978-3-322-91508-5-binding and esterase activities of HEF in vitro. The glycan-bind property is monitored through glycan microarray, MDCK cell-binding assay, Hemagglutination assay, solid-phase lectin binding assay, and immunofluorescence of tissue sections, and its esterase property is analyzed via esterase enzymatic activity assay.作者: congenial 時(shí)間: 2025-3-30 02:57 作者: Ankylo- 時(shí)間: 2025-3-30 04:14
Preparation of Recombinant PA Endonuclease Domain Protein of Influenza A Virus and Its Application omain protein was amplified with PCR and cloned into an expression vector. Six His-tagged PA endonuclease domain proteins were expressed in . and purified with Ni.-agarose resin and imidazole using an ion-exchange column. Using the recombinant PA endonuclease domain protein, an endonuclease assay was performed.作者: Moderate 時(shí)間: 2025-3-30 09:53 作者: gospel 時(shí)間: 2025-3-30 12:39
Receptor-Binding Assay for Avian Influenza Viruses,ylated Siaα2–3Gal glycans should be monitored for the better control of avian influenza in both poultry and wild birds. Here, general methods and points for the glycan-binding assay that are specifically modified to target fucosylated Siaα2–3Gal glycans are provided.作者: 赤字 時(shí)間: 2025-3-30 18:24 作者: Fissure 時(shí)間: 2025-3-30 22:41 作者: VICT 時(shí)間: 2025-3-31 03:44 作者: 全面 時(shí)間: 2025-3-31 05:24
Book 2022ractions. Chapters guide readers through glycan analysis, glycan distribution analysis, glycan and lectin microarray, preparation of recombinant viral domain protein, reverse genetics and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors.?Written in作者: 平息 時(shí)間: 2025-3-31 10:40
Book 2022als and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls...?Authoritative and cutting-edge,?.Glycovirology: Methods and Protocols?.aims?to be?an essential resource for researchers who wish to learn more about glycovirology...?.作者: PLUMP 時(shí)間: 2025-3-31 16:07 作者: GNAW 時(shí)間: 2025-3-31 20:06
,Traditionelle Ans?tze: haben Grenzen!,glycoprotein fetuin. Based on the concentration dependence of IV binding to the membrane, we estimate binding avidity and number of binding sites. We describe two variants of the assay in Subheadings 3.6 and 3.7 and provide examples.作者: HERTZ 時(shí)間: 2025-3-31 21:58
https://doi.org/10.1007/3-540-33020-8glutination assays to estimate virus particles’ binding specificity, hemadsorption assays to estimate virus glycoproteins’ binding specificity, and virus infectivity assays. These methods contribute to identifying the specificity of sialic acid lineage recognition of the hPIV or other sialic acid-binding viruses.作者: 過份 時(shí)間: 2025-4-1 02:05 作者: Peristalsis 時(shí)間: 2025-4-1 07:25
Preparation of Glycan Arrays Using Glycopeptides Derived From Biomaterials,ted using an evanescent-field-activated fluorescence scanner. It is clearly shown that H1N1 strains of influenza virus recognize BSA, to which DiNeuα2-6bianntena-peptide (SGP) is covalently linked, while on the other hand H5N1 strains recognize BSA linked to DiNeuα2-3bianntena-peptide (α2,3SGP).作者: Jubilation 時(shí)間: 2025-4-1 12:24 作者: Alienated 時(shí)間: 2025-4-1 14:49
Characterization of Human Parainfluenza Virus Receptor Using Terminal Sialic Acid Linkage-Modified glutination assays to estimate virus particles’ binding specificity, hemadsorption assays to estimate virus glycoproteins’ binding specificity, and virus infectivity assays. These methods contribute to identifying the specificity of sialic acid lineage recognition of the hPIV or other sialic acid-binding viruses.
