標(biāo)題: Titlebook: Glycosaminoglycans; Chemistry and Biolog Kuberan Balagurunathan,Hiroshi Nakato,Umesh R. Des Book 2015 Springer Science+Business Media New Y [打印本頁] 作者: memoir 時間: 2025-3-21 16:51
書目名稱Glycosaminoglycans影響因子(影響力)
書目名稱Glycosaminoglycans影響因子(影響力)學(xué)科排名
書目名稱Glycosaminoglycans網(wǎng)絡(luò)公開度
書目名稱Glycosaminoglycans網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Glycosaminoglycans被引頻次
書目名稱Glycosaminoglycans被引頻次學(xué)科排名
書目名稱Glycosaminoglycans年度引用
書目名稱Glycosaminoglycans年度引用學(xué)科排名
書目名稱Glycosaminoglycans讀者反饋
書目名稱Glycosaminoglycans讀者反饋學(xué)科排名
作者: Nonthreatening 時間: 2025-3-21 23:02 作者: 無能力之人 時間: 2025-3-22 01:19 作者: 帶來墨水 時間: 2025-3-22 06:32 作者: DEI 時間: 2025-3-22 09:14 作者: FOLD 時間: 2025-3-22 15:48 作者: FOLD 時間: 2025-3-22 18:13
https://doi.org/10.1007/978-3-642-96252-3nt, and difficulty of their preparation prohibit library synthesis in significant amounts. Herein, the use of bacterial heparin lyases for the production of HS oligosaccharides via enzymatic depolymerization of HS polymers is discussed. The separation and purification of these oligosaccharides by li作者: exceed 時間: 2025-3-23 01:12 作者: 放逐某人 時間: 2025-3-23 03:07
?Psychoreaktive“oder ?psychogene“St?rungenive cleavage with nitrous acid. The disaccharides formed by exhaustive degradation of the glycans is purified by gel-filtration chromatography on a Superdex-peptide column and analyzed by HPLC on a strong ion exchange Sax-Spherisorb column. 1H or 13C nuclear magnetic resonance spectroscopy in one or作者: FIN 時間: 2025-3-23 07:41 作者: SEEK 時間: 2025-3-23 10:30 作者: Expertise 時間: 2025-3-23 16:29
Book 2015ducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Glycosaminoglycans: Chemistry and Biology. serves as a valuable manual for cutting edge methodologies and practical tips to overcome any obstacles with experimentation pertaining t作者: BLA 時間: 2025-3-23 21:30
978-1-4939-4696-9Springer Science+Business Media New York 2015作者: considerable 時間: 2025-3-23 22:36
Glycosaminoglycans978-1-4939-1714-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: gangrene 時間: 2025-3-24 04:47 作者: 偶然 時間: 2025-3-24 09:50 作者: 可行 時間: 2025-3-24 13:58 作者: maroon 時間: 2025-3-24 16:09 作者: 省略 時間: 2025-3-24 21:13
Analysis of Hyaluronan Synthase Activity) are a family of three isoenzymes located on the plasma membrane that are responsible for the production of such polysaccharide and, therefore, their activity is critical to determine the accumulation of HA in tissues. Here, we describe a nonradioactive method to quantify the HAS enzymatic activity in crude cellular membrane preparation.作者: Homocystinuria 時間: 2025-3-25 00:25 作者: beta-carotene 時間: 2025-3-25 03:42
Abweichungen, Pathologie und Irreseinetic apparatus. The protocols for the gel preparation are based on the variations of the acrylamide/bisacrylamide and buffer concentrations. Different approaches for the extraction and purification of the disaccharides of various biologic samples and pharmaceutical preparations are also stressed.作者: FLACK 時間: 2025-3-25 09:13 作者: Accord 時間: 2025-3-25 12:46
Preparation of Isotope-Enriched Heparan Sulfate Precursors for Structural Biology Studiesion of stable isotope-enriched HS precursors has facilitated the HS structural analysis by NMR spectroscopy and mass spectrometry. Herein we describe a simple method to prepare isotopically enriched HS precursors.作者: chronology 時間: 2025-3-25 17:59 作者: 磨坊 時間: 2025-3-25 20:35
Capillary Electrophoretic Analysis of Isolated Sulfated Polysaccharides to Characterize Pharmaceuticulfate saccharides from urine, their digestion by deployment of heparinase enzymes (Ernst et al., Crit Rev Biochem Mol Biol 30:387–444, 1995), resolution of species through use of orthogonal digestions, and analysis of the resulting disaccharides by capillary electrophoresis.作者: 開玩笑 時間: 2025-3-26 00:54
Die einzelnen Geisteskrankheiten,bile linker. The linker cleavage from the resin is performed in a continuous-flow photoreactor under chemically mild conditions. The described approach will serve as a general scheme to systematically access oligosaccharides of all GAG families.作者: 外表讀作 時間: 2025-3-26 04:40 作者: 人工制品 時間: 2025-3-26 08:44 作者: dissent 時間: 2025-3-26 13:56
R?umliches Wahrnehmen und Vorstellenlic labeling of heparan sulfate with [.S]sulfate or [.H]glucosamine in cell cultures is described, in addition to characterization of polysaccharide chain length and degree of .-sulfation. Methods to measure NDST enzyme activity are also presented.作者: 打擊 時間: 2025-3-26 18:21
Automated Synthesis of Chondroitin Sulfate Oligosaccharidesbile linker. The linker cleavage from the resin is performed in a continuous-flow photoreactor under chemically mild conditions. The described approach will serve as a general scheme to systematically access oligosaccharides of all GAG families.作者: Ceramic 時間: 2025-3-26 22:44
Chemical Modification of Heparin and Heparosanle to generate polymers that can regulate biological processes beyond hemostasis. Here we describe a variety of simple chemical modification methods, .-acetylation, .-deacetylation, .-sulfation, .-sulfation, 2-. desulfation, and complete desulfation, to prepare heparin-like polymers with distinct sulfation patterns.作者: extract 時間: 2025-3-27 04:49 作者: Engulf 時間: 2025-3-27 05:42
Heparan Sulfate Structure: Methods to Study ,-Sulfation and NDST Actionlic labeling of heparan sulfate with [.S]sulfate or [.H]glucosamine in cell cultures is described, in addition to characterization of polysaccharide chain length and degree of .-sulfation. Methods to measure NDST enzyme activity are also presented.作者: nurture 時間: 2025-3-27 09:36
1064-3745 ation advice from the experts.Glycosaminoglycans: Chemistry and Biology. emphasizes several areas of glycosaminoglycan research especially analysis and application of GAGs using a variety of approaches.?Chapters focus on chemical and enzymatic synthesis of GAGs for therapeutic purposes; biophysical 作者: Laconic 時間: 2025-3-27 15:36
Die ?endogenen“ Geistesst?rungenfate donor in the sulfation of GAGs by sulfotransferases. Therefore, synthesis of PAPS carrying sulfur isotopes is critical in the preparation of labeled GAGs for biochemical studies. Here we describe a robust in vitro enzymatic synthesis of sulfur isotope-enriched PAPS which allows for heavy- or radio-isotope labeling of GAG chains.作者: Ibd810 時間: 2025-3-27 19:28 作者: 解開 時間: 2025-3-28 02:01 作者: 旁觀者 時間: 2025-3-28 04:16 作者: 頭盔 時間: 2025-3-28 08:35 作者: 寡頭政治 時間: 2025-3-28 10:24
Karl Westhoff,P. G. Zimbardo,F. L. Ruchrum, lens capsule) and has been validated. Analysis of biologic tissue samples is often more demanding in terms of detection sensitivity, and thus concentration pretreatment steps and/or a derivatization step with 2-aminoacridone are often advisable.作者: 上流社會 時間: 2025-3-28 17:16
Enzymatic Synthesis of Heparan Sulfate and Heparinnd can be used as a precursor in the enzymatic synthesis of HS-like polysaccharides. Here, we describe an enzymatic approach to synthesize several specifically sulfated HS polysaccharides for biological studies using the heparosan backbone and a combination of recombinant biosynthetic enzymes such as C5-epimerase and sulfotransferases.作者: COKE 時間: 2025-3-28 20:18 作者: GOAD 時間: 2025-3-29 00:23 作者: 吵鬧 時間: 2025-3-29 03:28 作者: 無聊的人 時間: 2025-3-29 07:13 作者: Alpha-Cells 時間: 2025-3-29 11:48
https://doi.org/10.1007/978-3-662-12243-3tion relations is, however, hampered due to the lack of availability of HS in sufficient quantities as well as the molecular heterogeneity of naturally occurring HS. Enzymatic synthesis of HS is an attractive alternative to the use of naturally occurring HS, as it reduces molecular heterogeneity, or作者: 猛擊 時間: 2025-3-29 17:55
https://doi.org/10.1007/978-3-642-96252-3 which bind their target proteins to induce conformational changes, compete with other ligands, or facilitate the formation of signaling complexes. One example, the extensively studied heparin pentasaccharide sequence—which binds antithrombin-III, inducing a conformational change that increases its 作者: Overdose 時間: 2025-3-29 21:04 作者: vasculitis 時間: 2025-3-30 00:47 作者: 抑制 時間: 2025-3-30 06:32
https://doi.org/10.1007/978-3-662-00125-7 studies of heparan sulfate are often challenging due to the heterogeneity and complexity of the HS molecules. Radioisotope metabolic labeling of HS in cellular systems has enabled the elucidation of HS structures as well as the interactions between HS and proteins. However, radiolabeled structures 作者: Notorious 時間: 2025-3-30 10:52 作者: 思考而得 時間: 2025-3-30 15:59
https://doi.org/10.1007/978-3-662-42402-5ne knockout, the ability to inhibit GAG biosynthesis would allow us to examine the function of endogenous GAG chains. However, ubiquitously and irreversibly knocking out all GAG biosynthesis would cause multiple effects making it difficult to attribute a specific biological role to a specific GAG st作者: Dictation 時間: 2025-3-30 16:48 作者: monogamy 時間: 2025-3-30 23:14
https://doi.org/10.1007/978-3-642-61775-1lgalactosamine) and a uronic acid (glucuronic acid or iduronic acid) or galactose. Glycosaminoglycans have sulfated residues at various positions except for hyaluronan, and those sulfated residues regulate the biological functions of a wide variety of proteins, primarily through high-affinity intera作者: 一大塊 時間: 2025-3-31 02:10
Die Einteilung der Geisteskrankheiten,lecular weight of heparin have been developed to assure the quality and consistency of heparin products for medicinal use, and these methods can be applied in other laboratory contexts. In the method described here, high-performance gel permeation chromatography is calibrated using appropriate hepar作者: 殺人 時間: 2025-3-31 07:35
Abweichungen, Pathologie und Irreseinbiosynthetic process that produces these linear polysaccharides in cells and tissues. Heparin and heparan sulfate play critical roles in normal physiology and pathophysiology; hence it is important to understand how their structural features may influence overall activity. Therefore, high-resolution作者: 雇傭兵 時間: 2025-3-31 10:07 作者: 猜忌 時間: 2025-3-31 16:14 作者: Physiatrist 時間: 2025-3-31 17:36
