標(biāo)題: Titlebook: Glyco-Engineering; Methods and Protocol Alexandra Castilho Book 2015 Springer Science+Business Media New York 2015 Carbohydrate composition [打印本頁(yè)] 作者: aspirant 時(shí)間: 2025-3-21 18:42
書目名稱Glyco-Engineering影響因子(影響力)
書目名稱Glyco-Engineering影響因子(影響力)學(xué)科排名
書目名稱Glyco-Engineering網(wǎng)絡(luò)公開度
書目名稱Glyco-Engineering網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Glyco-Engineering被引頻次
書目名稱Glyco-Engineering被引頻次學(xué)科排名
書目名稱Glyco-Engineering年度引用
書目名稱Glyco-Engineering年度引用學(xué)科排名
書目名稱Glyco-Engineering讀者反饋
書目名稱Glyco-Engineering讀者反饋學(xué)科排名
作者: 一條卷發(fā) 時(shí)間: 2025-3-21 20:53
Book 2015of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Authoritative and extensive, .Glyco-Engineering: Methods and Protocols. offers vast options to help researchers to choose the expression system an作者: Hla461 時(shí)間: 2025-3-22 00:58 作者: cardiovascular 時(shí)間: 2025-3-22 07:56 作者: FIN 時(shí)間: 2025-3-22 08:55 作者: PLE 時(shí)間: 2025-3-22 15:00 作者: PLE 時(shí)間: 2025-3-22 17:42
Progress in Yeast Glycosylation Engineeringtions. The addition of glycans is one such modification. Unfortunately, while certain aspects of glycosylation are shared between lower and higher eukaryotes, significant differences exist. Over the last two decades much research has focused on engineering the glycosylation pathways of yeast to more作者: 軟膏 時(shí)間: 2025-3-22 21:37 作者: Grating 時(shí)間: 2025-3-23 04:20 作者: 感情脆弱 時(shí)間: 2025-3-23 07:05
1064-3745 eproducible results.Contains key notes and implementation ad.Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of ca作者: Fabric 時(shí)間: 2025-3-23 10:36 作者: 擁護(hù)者 時(shí)間: 2025-3-23 14:31 作者: Ringworm 時(shí)間: 2025-3-23 19:51 作者: Anonymous 時(shí)間: 2025-3-24 02:14 作者: 改變 時(shí)間: 2025-3-24 06:13 作者: 生命 時(shí)間: 2025-3-24 07:47 作者: Budget 時(shí)間: 2025-3-24 11:38 作者: CBC471 時(shí)間: 2025-3-24 17:48
SweetBac: Applying MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylatione glycosylation potential of insect cells as expression hosts. The application of SweetBac, including the methods needed to investigate the glycosylation pattern of the purified recombinant protein, is described in this chapter.作者: 斜谷 時(shí)間: 2025-3-24 20:50 作者: 皺痕 時(shí)間: 2025-3-25 01:34 作者: outrage 時(shí)間: 2025-3-25 03:43
https://doi.org/10.1007/978-94-011-6123-7phosphate synthase (SAS), and wild-type CMP-sialic acid synthetase (CSAS) in the presence of GlcNAc leads to synthesis of CMP-sialic acids synthesis to support sialylation of N-glycans on glycoproteins.作者: 物種起源 時(shí)間: 2025-3-25 10:38
Electrical and Electronic Systems,A.) to avoid the formation of paucimannosidic glycans in insect S2 cells. In addition, we describe coexpression of β(1,4)-galactosyltransferase (GalT) as a strategy to improve N-glycosylation pattern and enable recombinant therapeutic proteins to be produced in S2 cells with more complex N-glycans.作者: OTHER 時(shí)間: 2025-3-25 13:51
gineering to obtain suitable platform lines for the production of recombinant proteins and biopharmaceuticals. Here we describe the methods which are effective for creating gene targeting constructs and transgenic moss lines as well as confirming successful homologous integration of the constructs and modification of target gene expression.作者: Aggregate 時(shí)間: 2025-3-25 18:42 作者: 職業(yè)拳擊手 時(shí)間: 2025-3-25 23:06 作者: 半球 時(shí)間: 2025-3-26 02:32
Engineering N-Glycosylation Pathway in Insect Cells: Suppression of β-,-Acetylglucosaminidase and ExA.) to avoid the formation of paucimannosidic glycans in insect S2 cells. In addition, we describe coexpression of β(1,4)-galactosyltransferase (GalT) as a strategy to improve N-glycosylation pattern and enable recombinant therapeutic proteins to be produced in S2 cells with more complex N-glycans.作者: Thrombolysis 時(shí)間: 2025-3-26 07:13 作者: 冷淡周邊 時(shí)間: 2025-3-26 08:45
Nuclear Reactions (Statistical Theory),aryotic glycan structures, completely novel protein glycoconjugates as well as to establish screening approaches for improvement and adaptation of the glycosylation machinery to specific applications.作者: 跟隨 時(shí)間: 2025-3-26 13:40
Current Approaches to Engineering ,-Linked Protein Glycosylation in Bacteriaaryotic glycan structures, completely novel protein glycoconjugates as well as to establish screening approaches for improvement and adaptation of the glycosylation machinery to specific applications.作者: 低三下四之人 時(shí)間: 2025-3-26 18:16 作者: Offbeat 時(shí)間: 2025-3-26 22:34 作者: Invertebrate 時(shí)間: 2025-3-27 02:29
Glyco-engineering O-Antigen-Based Vaccines and Diagnostics in , biotechnologically relevant host .. Here we provide insight on how to select the best strains and plasmids. We also describe methods for determination of glycan expression and assembly, protein glycosylation using western blot, and preparation of samples for mass spectrometry.作者: pus840 時(shí)間: 2025-3-27 09:10 作者: Munificent 時(shí)間: 2025-3-27 11:20 作者: 背心 時(shí)間: 2025-3-27 14:22 作者: LARK 時(shí)間: 2025-3-27 19:25 作者: 連鎖,連串 時(shí)間: 2025-3-28 01:27
Glyco-engineering O-Antigen-Based Vaccines and Diagnostics in ,new possibilities for glyco-engineering have arisen, and a wide array of glycans can be designed and potentially transferred to target proteins in the biotechnologically relevant host .. Here we provide insight on how to select the best strains and plasmids. We also describe methods for determinatio作者: 在駕駛 時(shí)間: 2025-3-28 03:38
Progress in Yeast Glycosylation Engineering used as model organisms to facilitate our understanding of such features and processes. To this end, a large number of powerful genetic tools have been developed to investigate and manipulate these organisms. Going hand-in-hand with these genetic tools is the ability to efficiently scale up the fer作者: 殺子女者 時(shí)間: 2025-3-28 09:44
Protein Production with a , OCH1 Knockout Strain in Fed-Batch Mode the target product describes a glycoprotein, an α-1,6-mannosyltransferase located in the Golgi apparatus of ., called OCH1, triggers hypermannosylation of the recombinant protein which significantly impedes following unit operations and hampers biopharmaceutical product applications. A knockout of 作者: 恃強(qiáng)凌弱的人 時(shí)間: 2025-3-28 13:10 作者: 艱苦地移動(dòng) 時(shí)間: 2025-3-28 17:48
Development of a Valuable Yeast Strain Using a Novel Mutagenesis Technique for the Effective Productncoded on a proofreading-deficient pol3 gene. This novel mutagenesis technique generates a pool of mutants that includes a no-mutant strain together with mutant strains carrying multiple mutations. By using a suitable screening system it is possible to isolate the desired mutant strain from this poo作者: Medley 時(shí)間: 2025-3-28 19:46 作者: Insulin 時(shí)間: 2025-3-29 02:53
SweetBac: Applying MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylationerences in numerous proteins involved in biosynthesis of the relevant glycan chains in these species. The need for efficient, robust and flexible methods for recombinant expression of proteins is met by the recently described MultiBac technology, an advanced and optimized baculovirus-based system fo作者: 委屈 時(shí)間: 2025-3-29 03:29 作者: 音樂(lè)等 時(shí)間: 2025-3-29 08:19
Engineering N-Glycosylation Pathway in Insect Cells: Suppression of β-,-Acetylglucosaminidase and Ex β-.-acetylglucosaminidase (GlcNAcase), a hexosaminidase in the Golgi membrane which removes a terminal .-acetylglucosamine (GlcNAc), might contribute to simple N-glycosylation profile in several insect cells including . S2. Here, we describe GlcNAcase suppression strategy using RNA interference (RN作者: 廢除 時(shí)間: 2025-3-29 12:30
N-Glyco-Engineering in Plants: Update on Strategies and Major Achievementse complex proteins from human origin, one issue that needs to be addressed is their glycan structure. In the past years we have been witnessing outstanding results in targeted manipulation of the plant .-glycosylation pathway allowing recombinant proteins to be produced with human-type oligosacchari作者: 流出 時(shí)間: 2025-3-29 16:59
Gene Targeting for Precision Glyco-Engineering: Production of Biopharmaceuticals Devoid of Plant-Typtowards a humanized form. Posttranslational modifications in plants are similar to those in humans, but several differences affect product quality and efficacy and can also cause immune responses in patients. In the moss . highly efficient gene targeting via homologous recombination enables glyco-en作者: synovium 時(shí)間: 2025-3-29 23:23 作者: 彎腰 時(shí)間: 2025-3-30 03:52
Heinz Rutishauser,Martin Gutknechtget organism. This methodology has been successfully applied towards enhancing the N-linked glycosylation efficiency of .. Here, we describe the generation of differentially sized libraries from the . W3110 genome followed by high-throughput semiquantitative glycan specific screening. DNA sequenced 作者: 小卷發(fā) 時(shí)間: 2025-3-30 05:01 作者: 輕打 時(shí)間: 2025-3-30 08:52 作者: CHURL 時(shí)間: 2025-3-30 16:00
Notation and Mathematical Preliminaries, used as model organisms to facilitate our understanding of such features and processes. To this end, a large number of powerful genetic tools have been developed to investigate and manipulate these organisms. Going hand-in-hand with these genetic tools is the ability to efficiently scale up the fer作者: GOAT 時(shí)間: 2025-3-30 17:04 作者: 不如屎殼郎 時(shí)間: 2025-3-30 21:08 作者: ORE 時(shí)間: 2025-3-31 01:38
Lectures on Several Complex Variablesncoded on a proofreading-deficient pol3 gene. This novel mutagenesis technique generates a pool of mutants that includes a no-mutant strain together with mutant strains carrying multiple mutations. By using a suitable screening system it is possible to isolate the desired mutant strain from this poo作者: Anhydrous 時(shí)間: 2025-3-31 07:49 作者: COST 時(shí)間: 2025-3-31 09:43
https://doi.org/10.1007/978-93-86279-58-3erences in numerous proteins involved in biosynthesis of the relevant glycan chains in these species. The need for efficient, robust and flexible methods for recombinant expression of proteins is met by the recently described MultiBac technology, an advanced and optimized baculovirus-based system fo作者: Rebate 時(shí)間: 2025-3-31 14:03
https://doi.org/10.1007/978-94-011-6123-7 a method to engineer CMP-sialic acid synthesis capability into . (Sf9) cells, a prototypical insect cell line, by recombinant expression of sialic acid synthesis pathway genes using baculovirus technology. Co-expression of a . UDP-GlcNAc-2-epimerase/ManNAc kinase (EKR263L), wild-type sialic acid 9-
