標(biāo)題: Titlebook: Glucose Transport; Methods and Protocol Karin Lindkvist-Petersson,Jesper S‘Hansen Book 2018 Springer Science+Business Media LLC 2018 GLUTs. [打印本頁] 作者: ANNOY 時間: 2025-3-21 17:40
書目名稱Glucose Transport影響因子(影響力)
書目名稱Glucose Transport影響因子(影響力)學(xué)科排名
書目名稱Glucose Transport網(wǎng)絡(luò)公開度
書目名稱Glucose Transport網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Glucose Transport被引頻次
書目名稱Glucose Transport被引頻次學(xué)科排名
書目名稱Glucose Transport年度引用
書目名稱Glucose Transport年度引用學(xué)科排名
書目名稱Glucose Transport讀者反饋
書目名稱Glucose Transport讀者反饋學(xué)科排名
作者: Abutment 時間: 2025-3-21 22:34 作者: Esophagitis 時間: 2025-3-22 02:11 作者: 壯麗的去 時間: 2025-3-22 07:03 作者: Neutral-Spine 時間: 2025-3-22 10:41 作者: exclusice 時間: 2025-3-22 15:18
Karin Lindkvist-Petersson,Jesper S‘HansenIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia作者: exclusice 時間: 2025-3-22 18:18 作者: 流動才波動 時間: 2025-3-22 21:13 作者: dissolution 時間: 2025-3-23 03:15
Jeffrey A. Cardille,Monica G. Turnerput screening. We generated a strain deficient for all endogenous hexose transporters, which has been successfully used to clone, characterize, and engineer carbohydrate transporters from different source organisms. Here we present basic protocols for handling this strain and characterizing sugar transporters heterologously expressed in it.作者: HAWK 時間: 2025-3-23 07:34 作者: FOVEA 時間: 2025-3-23 13:16 作者: Synapse 時間: 2025-3-23 17:24 作者: Obstacle 時間: 2025-3-23 21:18
A Growth-Based Screening System for Hexose Transporters in Yeast,put screening. We generated a strain deficient for all endogenous hexose transporters, which has been successfully used to clone, characterize, and engineer carbohydrate transporters from different source organisms. Here we present basic protocols for handling this strain and characterizing sugar transporters heterologously expressed in it.作者: 柏樹 時間: 2025-3-24 00:03 作者: 軟膏 時間: 2025-3-24 02:44 作者: exceed 時間: 2025-3-24 06:43
GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis,sporter GLUT2 act as glucose sensors in different regions of the brain and contribute to the control of glucose homeostasis and feeding behavior. In this chapter we describe the methods used to explore glucose sensing in genetically tagged GLUT2-expressing neurons with slice electrophysiology.作者: 征兵 時間: 2025-3-24 13:56
Book 2018ook cover topics such as expression and purification of glucose transporters in heterologous expression systems, crystal structure determination, glucose transport activity assays in cellular and synthetic model systems, investigation of the GLUT4 membrane trafficking machinery by novel labeling str作者: 航海太平洋 時間: 2025-3-24 16:20 作者: FISC 時間: 2025-3-24 20:08 作者: 金盤是高原 時間: 2025-3-25 00:44 作者: 移植 時間: 2025-3-25 05:50 作者: 青石板 時間: 2025-3-25 09:12
Expression and Purification of Rat Glucose Transporter 1 in ,, in shaker flasks, as commonly performed in academic research laboratories, results in relatively high yields of membrane protein. The purification protocol describes all steps needed to obtain a pure and homogenous GLUT1 protein solution, including cell growth, membrane isolation, and chromatographic purification methods.作者: Obliterate 時間: 2025-3-25 14:03 作者: Banquet 時間: 2025-3-25 18:09
Glucose Uptake in Heterologous Expression Systems,hrough a previously described radiolabeled glucose transport assay. Although this discussion is focused on the GLUT1 transporter, these techniques are easily transferrable to other glucose transporters.作者: novelty 時間: 2025-3-25 23:40 作者: Halfhearted 時間: 2025-3-26 03:20
GLUT Characterization Using Frog , Oocytes,expression level by biotinylation and Western blotting analysis. Finally, we also describe how GLUT9-expressing oocytes can be used to measure urate kinetics by radioisotopes as well as two-microelectrode voltage clamping techniques.作者: BUOY 時間: 2025-3-26 05:14 作者: Alopecia-Areata 時間: 2025-3-26 11:12
Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level,ofluidic systems are fabricated and how they are utilized. Finally, we discuss how the large amount of data can be analyzed in a “semi-automatic” manner using custom-made software. In summary, we provide a guide to how to use microfluidic systems in single-cell studies.作者: elastic 時間: 2025-3-26 13:18 作者: 轉(zhuǎn)換 時間: 2025-3-26 19:52
1064-3745 ation advice from the experts.Includes supplementary materiaThis volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologo作者: 兇猛 時間: 2025-3-26 21:54 作者: miniature 時間: 2025-3-27 04:46
Anton Bogdanovych,Tomas Trescakbased on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assay is applicable for the functional analysis of a variety of hexose-transporting proteins. We furthermore believe that it can aid in the development of drugs targeting hexose transporters.作者: intrigue 時間: 2025-3-27 08:08 作者: 滲入 時間: 2025-3-27 12:28
Ancient Assets for the Twenty-First Centuryer construct allows for visualization of GLUT4 specifically at the cell surface or GLUT4 that has recycled from the cell surface while simultaneously marking the total GLUT4 pool. Here, we discuss and outline the general application of this reporter construct and its use in evaluating GLUT4 translocation within cultured adipocytes.作者: Migratory 時間: 2025-3-27 16:06
https://doi.org/10.1007/978-1-4842-5058-7hapter, we present the detailed protocols of recombinant protein expression, purification, and crystallization of GLUT1 and GLUT3, which may help the pursuit of structural elucidation of other eukaryotic membrane proteins.作者: 他很靈活 時間: 2025-3-27 19:58
Conditionals, Recursion, and Loops Seahorse bioenergetics analyzer can measure both the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). The proposed methodology affords a robust, high-throughput method to screen for GLUT inhibition in cells engineered to express specific GLUTs, providing live cell read-outs upon GLUT inhibition.作者: 名義上 時間: 2025-3-28 01:32
Mouse Control: A Card Game Agnesestrogen receptor (ER) ligands, which were structurally optimized in order to direct their activity towards GLUT1 and to minimize their binding to the ERs, leading to the production of efficient and selective inhibitors of glucose uptake in cancer cells.作者: constellation 時間: 2025-3-28 04:21 作者: Malfunction 時間: 2025-3-28 07:47 作者: cavity 時間: 2025-3-28 14:16 作者: interrupt 時間: 2025-3-28 16:27
Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3,hapter, we present the detailed protocols of recombinant protein expression, purification, and crystallization of GLUT1 and GLUT3, which may help the pursuit of structural elucidation of other eukaryotic membrane proteins.作者: nepotism 時間: 2025-3-28 19:06
Evaluating the Efficacy of GLUT Inhibitors Using a Seahorse Extracellular Flux Analyzer, Seahorse bioenergetics analyzer can measure both the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). The proposed methodology affords a robust, high-throughput method to screen for GLUT inhibition in cells engineered to express specific GLUTs, providing live cell read-outs upon GLUT inhibition.作者: 恩惠 時間: 2025-3-29 01:07
Design, Synthesis, and Evaluation of GLUT Inhibitors,estrogen receptor (ER) ligands, which were structurally optimized in order to direct their activity towards GLUT1 and to minimize their binding to the ERs, leading to the production of efficient and selective inhibitors of glucose uptake in cancer cells.作者: Trochlea 時間: 2025-3-29 03:19
GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by Immunofluorescence,ocal fluorescence microscopy to quantify cell surface GLUT4. or GLUT4.-GFP in cells co-transfected with diverse cDNA constructs, treated with siRNAs, or co-stained with antibodies for other proteins of interest. Herein, we describe the methodology to perform these experimental approaches in insulin-stimulated L6 muscle cells.作者: 昏迷狀態(tài) 時間: 2025-3-29 11:15
Proximity Ligation Assay to Study the GLUT4 Membrane Trafficking Machinery,lex and/or with the SNARE proteins individually. Studying the interactions that occur between SNARE proteins themselves and also with Munc18c in insulin-responsive cells is critical to further understand SNARE protein function and GLUT4 trafficking mechanism in general.作者: 慢慢流出 時間: 2025-3-29 13:32
Tracking GLUT2 Translocation by Live-Cell Imaging,uantify GLUT2 translocation, and its dynamics, by live imaging of a mCherry-hGLUT2 fusion protein in polarized epithelial cells. This system enables testing of putative modulators of GLUT2 translocation, which are potential drugs for conditions of impaired glucose homeostasis and associated nephropathy.作者: Bridle 時間: 2025-3-29 18:14 作者: MOTIF 時間: 2025-3-29 20:17 作者: Dorsal-Kyphosis 時間: 2025-3-30 02:39
https://doi.org/10.1007/978-1-349-07793-9itated by fluorescence-based screening approaches. We describe (1) the strategy and protocol of cloning by homologous recombination, (2) whole-cell and in-gel fluorescence measurements to estimate GLUT-GFP fusion protein yields, (3) use of size-exclusion chromatography monitored by fluorescence (FSE作者: abolish 時間: 2025-3-30 04:43
https://doi.org/10.1007/978-3-319-68072-9rovide a detailed protocol on oocyte extraction and preparation for GLUT9 protein expression. Furthermore, we describe the determination of GLUT9 overexpression level by biotinylation and Western blotting analysis. Finally, we also describe how GLUT9-expressing oocytes can be used to measure urate k作者: filicide 時間: 2025-3-30 09:08 作者: disciplined 時間: 2025-3-30 12:30 作者: archenemy 時間: 2025-3-30 17:25 作者: 在前面 時間: 2025-3-31 00:42 作者: 引起 時間: 2025-3-31 01:27
Jeffrey A. Cardille,Monica G. Turnerology of biological cells after uptake of glucose. Here, we briefly discuss the advantages of using microfluidic systems. We further describe how microfluidic systems are fabricated and how they are utilized. Finally, we discuss how the large amount of data can be analyzed in a “semi-automatic” mann作者: 充滿裝飾 時間: 2025-3-31 06:16
Jeffrey A. Cardille,Monica G. Turnerput screening. We generated a strain deficient for all endogenous hexose transporters, which has been successfully used to clone, characterize, and engineer carbohydrate transporters from different source organisms. Here we present basic protocols for handling this strain and characterizing sugar tr作者: 悅耳 時間: 2025-3-31 13:04 作者: 正面 時間: 2025-3-31 13:24
https://doi.org/10.1007/978-3-658-34495-5e last decade, the method has successfully been used to explore GLUT4 translocation in adipocytes. Here, we describe the procedure for studying GLUT4 trafficking using TIRF microscopy in isolated primary adipocytes.作者: pester 時間: 2025-3-31 19:00
Ancient Assets for the Twenty-First Centuryas become an important tool in the assessment of GLUT4 recycling in cultured adipocytes and myocytes. Through the use of light microscopy, this reporter construct allows for visualization of GLUT4 specifically at the cell surface or GLUT4 that has recycled from the cell surface while simultaneously 作者: FECT 時間: 2025-3-31 23:42
Leslie P. Steffe,Heide G. Wiegeln and muscle contraction to achieve a new steady state with more GLUT4 proteins at the muscle cell surface. To gain a better understanding of the molecular and cellular mechanisms that govern GLUT4 protein recycling, we developed an in vitro model in which .-epitope-tagged GLUT4 or GLUT4-GFP is expr作者: 斗爭 時間: 2025-4-1 02:44 作者: 背帶 時間: 2025-4-1 07:01 作者: choroid 時間: 2025-4-1 12:59
Lesbian Chic in the Digital World,hanging carbohydrate levels. The facilitative GLUT2 transporter is uniquely expressed in metabolic epithelial cells of the intestine, pancreas, liver, and kidney. GLUT2 dysfunction is associated with several pathologies, including Fanconi-Bickel syndrome, a glycogen storage disease, characterized by作者: Coterminous 時間: 2025-4-1 16:57
Marianne Ekman,Beth Maina Ahlbergsporter GLUT2 act as glucose sensors in different regions of the brain and contribute to the control of glucose homeostasis and feeding behavior. In this chapter we describe the methods used to explore glucose sensing in genetically tagged GLUT2-expressing neurons with slice electrophysiology.作者: filial 時間: 2025-4-1 19:28
Expression and Purification of Rat Glucose Transporter 1 in ,,ving the three-dimensional protein structure. Here we describe the production of the rat glucose transporter 1 (GLUT1), a membrane protein facilitating the transport of glucose in cells. The protein is recombinantly expressed in the yeast .. It is easily maintained and large-scale protein production
