作者: 大雨 時(shí)間: 2025-3-21 23:31 作者: 大門(mén)在匯總 時(shí)間: 2025-3-22 01:48 作者: encyclopedia 時(shí)間: 2025-3-22 07:03 作者: 水土 時(shí)間: 2025-3-22 10:59
Methods for Studying the Germline of the Human Parasite ,,作者: 拱形大橋 時(shí)間: 2025-3-22 13:23 作者: 拱形大橋 時(shí)間: 2025-3-22 18:12 作者: Munificent 時(shí)間: 2025-3-22 21:50 作者: 載貨清單 時(shí)間: 2025-3-23 02:44 作者: Ascendancy 時(shí)間: 2025-3-23 05:53
Depth of Shower Maximum and Elongation Rateing over 5?days. The hPGCLCs can be isolated using specific cell surface markers without the need for generating germ cell-specific reporter hPSC lines. This powerful method for the induction and isolation of hPGCLCs can be applied to both hESCs and iPSCs, which can be used for advances in human ger作者: 運(yùn)動(dòng)吧 時(shí)間: 2025-3-23 10:51 作者: Afflict 時(shí)間: 2025-3-23 16:50 作者: 徹底檢查 時(shí)間: 2025-3-23 21:15 作者: COKE 時(shí)間: 2025-3-24 02:14
Extensions of Rings and Modulesme integrity of the germline. Here we describe detailed procedures of how to produce monoclonal antibodies against a mouse nuclear PIWI protein, MIWI2, which functions in de novo DNA methylation of target transposon loci. We then describe how to use the antibodies to isolate associated complexes and to detect MIWI2 immunohistochemically.作者: Mettle 時(shí)間: 2025-3-24 02:21 作者: 脖子 時(shí)間: 2025-3-24 10:28 作者: ELUDE 時(shí)間: 2025-3-24 13:30 作者: 創(chuàng)新 時(shí)間: 2025-3-24 15:55
Identification of Mouse piRNA Pathway Components Using Anti-MIWI2 Antibodies,me integrity of the germline. Here we describe detailed procedures of how to produce monoclonal antibodies against a mouse nuclear PIWI protein, MIWI2, which functions in de novo DNA methylation of target transposon loci. We then describe how to use the antibodies to isolate associated complexes and to detect MIWI2 immunohistochemically.作者: 合適 時(shí)間: 2025-3-24 19:32 作者: MIRTH 時(shí)間: 2025-3-25 00:47 作者: lactic 時(shí)間: 2025-3-25 04:20
https://doi.org/10.1007/978-1-4842-8170-3technique for the acquisition of 4D datasets of the . ovariole for periods that can exceed 12 consecutive hours. Live-cell imaging facilitates the investigation of molecular and cellular dynamics that were not previously possible using still images.作者: APO 時(shí)間: 2025-3-25 09:10
Script Component Transformation method to analyze poly(A) tail lengths of specific mRNAs in this tissue is valuable. Several methods of poly(A) test (PAT) assays have been reported to measure mRNA poly(A) tail lengths in vivo. Here, we describe two of these methods (PAT and ePAT) that we have adapted for . ovarian germ cells and germ-line stem cells.作者: placebo-effect 時(shí)間: 2025-3-25 14:06
Per Andersson,Lars-Gunnar Mattssonmethod is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect . mRNA and immunofluorescence to detect the pan-germ cell marker Vasa.作者: 使高興 時(shí)間: 2025-3-25 19:16 作者: Obstreperous 時(shí)間: 2025-3-25 21:16 作者: CBC471 時(shí)間: 2025-3-26 03:20
Measurement of mRNA Poly(A) Tail Lengths in , Female Germ Cells and Germ-Line Stem Cells, method to analyze poly(A) tail lengths of specific mRNAs in this tissue is valuable. Several methods of poly(A) test (PAT) assays have been reported to measure mRNA poly(A) tail lengths in vivo. Here, we describe two of these methods (PAT and ePAT) that we have adapted for . ovarian germ cells and germ-line stem cells.作者: 災(zāi)難 時(shí)間: 2025-3-26 08:18
Identification of Germ-Line Stem Cells in Zebrafish,method is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect . mRNA and immunofluorescence to detect the pan-germ cell marker Vasa.作者: CLASH 時(shí)間: 2025-3-26 11:49 作者: ZEST 時(shí)間: 2025-3-26 15:17
Analysis of the , Germline Stem Cell Pool,the germline in living and fixed worms, cell cycle analysis, and analysis of markers. We also discuss assays to separate mutants that affect the stem cell vs. differentiation decision from those that affect germ cell processes more generally.作者: 清晰 時(shí)間: 2025-3-26 17:18
https://doi.org/10.1007/978-1-4471-0963-1 we describe the use of a monoclonal antibody to Pax7 as a means to detect spermatogonial stem cells (SSCs) both in tissue sections and in intact seminiferous tubules. Furthermore, we describe methods for lineage tracing as an alternative method to visualize Pax7. spermatogonial stem cells and their progeny.作者: Gerontology 時(shí)間: 2025-3-27 00:05 作者: Defraud 時(shí)間: 2025-3-27 01:59
Visualization and Lineage Tracing of Pax7+ Spermatogonial Stem Cells in the Mouse, we describe the use of a monoclonal antibody to Pax7 as a means to detect spermatogonial stem cells (SSCs) both in tissue sections and in intact seminiferous tubules. Furthermore, we describe methods for lineage tracing as an alternative method to visualize Pax7. spermatogonial stem cells and their progeny.作者: 和平主義 時(shí)間: 2025-3-27 07:32 作者: Musculoskeletal 時(shí)間: 2025-3-27 11:53 作者: custody 時(shí)間: 2025-3-27 15:31 作者: 可用 時(shí)間: 2025-3-27 18:44 作者: NEG 時(shí)間: 2025-3-27 23:11 作者: 帶子 時(shí)間: 2025-3-28 05:08 作者: maculated 時(shí)間: 2025-3-28 08:16
Introduction to Flash Extensibility,echnical difficulties in isolating early larval ovaries. In addition, purifying RNA from larval ovaries proves to be more challenging than purifying it from other organs. Here we describe a technique for dissecting ovaries from early larvae and advise on how to extract RNA with maximum yield and pur作者: 根除 時(shí)間: 2025-3-28 13:21
https://doi.org/10.1007/978-1-4842-8170-3ls and their somatic cell neighbors can be identified at single-cell resolution within their native environment. Here we describe a fluorescent-based technique for the acquisition of 4D datasets of the . ovariole for periods that can exceed 12 consecutive hours. Live-cell imaging facilitates the inv作者: FECK 時(shí)間: 2025-3-28 15:13 作者: abstemious 時(shí)間: 2025-3-28 22:13
Per Andersson,Lars-Gunnar Mattsson specific regions in the gonads and can be identified because they uniquely express the . gene, which encodes a conserved regulator of translation. A method is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in sit作者: 賞錢(qián) 時(shí)間: 2025-3-29 00:42 作者: 過(guò)渡時(shí)期 時(shí)間: 2025-3-29 03:32 作者: 無(wú)可爭(zhēng)辯 時(shí)間: 2025-3-29 07:19
https://doi.org/10.1007/978-1-4471-0963-1 included the lack of specific markers and methods for lineage tracing of A. spermatogonia and their subsets. Immunolocalization of proteins in tissue sections has been a standard tool for the in situ identification and visualization of rare cellular subsets. However, these studies are limited by th作者: Intentional 時(shí)間: 2025-3-29 14:47 作者: 難取悅 時(shí)間: 2025-3-29 18:13
https://doi.org/10.1007/978-1-4612-3712-9ell-specific gene and protein expression, as well as determine direct effects of signaling molecules, it is necessary to prepare enriched populations of germ cells and maintain them in culture for several hours to multiple days. The protocols in this chapter are designed to provide a guide for the i作者: 慢慢流出 時(shí)間: 2025-3-29 20:06 作者: Constituent 時(shí)間: 2025-3-30 01:47 作者: 勉強(qiáng) 時(shí)間: 2025-3-30 04:02 作者: DEAWL 時(shí)間: 2025-3-30 10:27
https://doi.org/10.1007/978-1-4939-4017-2Germline stem cell; primordial germ cells; adult stem cells; live-cell imaging techniques; improved cell作者: 大廳 時(shí)間: 2025-3-30 15:19 作者: Pulmonary-Veins 時(shí)間: 2025-3-30 18:34
978-1-4939-8154-0Springer Science+Business Media New York 2017作者: Colonnade 時(shí)間: 2025-3-30 21:58
Germline Stem Cells978-1-4939-4017-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 烤架 時(shí)間: 2025-3-31 02:45 作者: Musket 時(shí)間: 2025-3-31 07:39
