標題: Titlebook: Germline Stem Cells; Methods and Protocol Michael Buszczak Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under e [打印本頁] 作者: IU421 時間: 2025-3-21 16:42
書目名稱Germline Stem Cells影響因子(影響力)
書目名稱Germline Stem Cells影響因子(影響力)學(xué)科排名
書目名稱Germline Stem Cells網(wǎng)絡(luò)公開度
書目名稱Germline Stem Cells網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Germline Stem Cells被引頻次
書目名稱Germline Stem Cells被引頻次學(xué)科排名
書目名稱Germline Stem Cells年度引用
書目名稱Germline Stem Cells年度引用學(xué)科排名
書目名稱Germline Stem Cells讀者反饋
書目名稱Germline Stem Cells讀者反饋學(xué)科排名
作者: Constitution 時間: 2025-3-21 20:51
Michael BuszczakIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: backdrop 時間: 2025-3-22 01:30 作者: 不可侵犯 時間: 2025-3-22 08:34
Medienkonkurrenz und Massenkommunikatione regulatory complexes, containing an Argonaute protein (AGO) guided by a bound sRNA, have the potential to regulate thousands of individual target transcripts at both the co- and post-transcriptional level. Determining the repertoire of transcripts that an AGO is capable of regulating in a particul作者: conception 時間: 2025-3-22 12:23 作者: 清楚 時間: 2025-3-22 16:30 作者: 清楚 時間: 2025-3-22 19:56 作者: 支柱 時間: 2025-3-22 23:22 作者: 清洗 時間: 2025-3-23 01:48
Cynthia Blomquist Gustavson MSW, ACSW, LCSW and cell type markers in combination with various genetic tools available for the . system. A major weakness of this system has been the difficulty of approaches for obtaining material for biochemical assays, proteomics, and genomic or transcriptomic profiling due to small-size and complex tissues.作者: reject 時間: 2025-3-23 08:54
,Lebensstile im sozial?kologischen Kontext,on as a conserved mechanism for replenishing stem cell pools after their loss, thereby maintaining tissue homeostasis. The testis of the fruit fly . offers a simple but powerful system to study dedifferentiation, the process by which differentiating spermatogonia can revert their fate to become full作者: MIRTH 時間: 2025-3-23 09:55
https://doi.org/10.1007/978-3-030-27492-4to build comprehensive cell-type-specific transcriptomic atlases for a wide range of tissues in several model organisms in order to discover cell-type-specific markers and drivers of gene expression. One such tissue is the ovary of the fruit-fly ., which is a popular model system with wide-ranging a作者: 甜得發(fā)膩 時間: 2025-3-23 15:39
Botanical Pesticides as Biocontrol Productsspecific regions in the gonads and can be identified because they uniquely express the . gene, which encodes a conserved regulator of translation. A method is presented here for identifying germline stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ 作者: 對手 時間: 2025-3-23 21:08
https://doi.org/10.1007/978-3-658-27172-5imordial germ cells (PGCs) that will generate the oogonia. First, these cells proliferate mitotically, and then they trigger the meiotic program and initiate meiotic prophase I. Since these processes happen during gestation, their study had been very limited and challenging. Recently, we reported th作者: COUCH 時間: 2025-3-23 22:56
David Jou,José Casas-Vázquez,Georgy Lebonn of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analysis of whole ovarian tissue in 3D throughout murine development and aging.作者: 熄滅 時間: 2025-3-24 02:42
David Jou,José Casas-Vázquez,Georgy Lebontudy germ cell-specific gene and protein expression, as well as determine direct effects of signaling molecules, it is necessary to prepare enriched populations of germ cells and maintain them in culture for several hours to multiple days. The protocols in this chapter are designed to provide a guid作者: 青春期 時間: 2025-3-24 08:52
David Jou,José Casas-Vázquez,Georgy Lebonment. Consequently, methods to culture mammalian stem cells through spermatogenesis in defined systems have not been established. Lack of success at culturing mammalian stem cells through spermatogenesis in defined systems reflects an inability to experimentally recapitulate biochemical events that 作者: 序曲 時間: 2025-3-24 12:32
Extended Irreversible Thermodynamicsvelopment, limiting the ability to study fundamental developmental steps in human reproductive biology. However, recent advancements in generating in-vitro models of gametogenesis have allowed the field to generate human primordial germ cell-like cells (hPGCLCs). In this chapter, we will review the 作者: TIGER 時間: 2025-3-24 16:32
Extended Linear Chain Compounds PSCs with distinct properties. By modulating the FGF, TGF-β, and WNT pathways, we have derived intermediate PSCs (FTW-PSCs) that are permissive for direct primordial germ cell-like cell (PGC-LC) induction in vitro. Here, we describe the method for derivation and maintenance of mouse and human FTW-P作者: 尾巴 時間: 2025-3-24 21:13
978-1-0716-3261-1The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: 恩惠 時間: 2025-3-25 00:04 作者: Fretful 時間: 2025-3-25 06:43 作者: Sinus-Rhythm 時間: 2025-3-25 10:03 作者: 性行為放縱者 時間: 2025-3-25 13:23 作者: 杠桿支點 時間: 2025-3-25 19:14
Targeting Endogenous Loci That Function in , Germline Stem Cells,e other genetic manipulations is useful for studying germline stem cell biology. In this chapter, we will describe a method to use CRISPR-Cas9 genome editing technology to make knock-out and knock-in flies. We will cover everything from guideRNA (gRNA) and donor plasmid design and cloning to screening for positive edits.作者: thyroid-hormone 時間: 2025-3-25 20:49 作者: 名義上 時間: 2025-3-26 01:02
Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells, PSCs with distinct properties. By modulating the FGF, TGF-β, and WNT pathways, we have derived intermediate PSCs (FTW-PSCs) that are permissive for direct primordial germ cell-like cell (PGC-LC) induction in vitro. Here, we describe the method for derivation and maintenance of mouse and human FTW-PSCs, as well as PGC-LC induction from FTW-PSCs.作者: START 時間: 2025-3-26 06:56 作者: 內(nèi)向者 時間: 2025-3-26 09:18 作者: 窗簾等 時間: 2025-3-26 13:15 作者: TERRA 時間: 2025-3-26 20:48 作者: Minuet 時間: 2025-3-26 23:53
https://doi.org/10.1007/978-3-476-03131-0g the . GSC pool and early stages of germ cell differentiation. The methods include examination of germlines in living and fixed worms, cell cycle analysis, and analysis of markers. We also discuss assays to separate mutant phenotypes that affect the stem cell vs. differentiation decision from those that affect germ cell processes more generally.作者: 割讓 時間: 2025-3-27 03:54
Cynthia Blomquist Gustavson MSW, ACSW, LCSWhe reliability of the results. Here, we provide our semi-optimized protocol of enrichment of?undifferentiated germ cells and somatic cells from non-tumorous?. testis, which we have successfully improved after multiple trials.作者: 無聊的人 時間: 2025-3-27 07:04 作者: Suggestions 時間: 2025-3-27 09:42 作者: 鋸齒狀 時間: 2025-3-27 15:30
Enrichment of Undifferentiated Germline and Somatic Cells from , Testes,he reliability of the results. Here, we provide our semi-optimized protocol of enrichment of?undifferentiated germ cells and somatic cells from non-tumorous?. testis, which we have successfully improved after multiple trials.作者: 一起平行 時間: 2025-3-27 19:26 作者: 無法取消 時間: 2025-3-27 22:41 作者: ALIAS 時間: 2025-3-28 03:40
,Lebensstile im sozial?kologischen Kontext, sibling GSCs. To facilitate the study of the cellular and molecular mechanisms underlying the process of germline dedifferentiation in the . testis, here we describe techniques for inducing high rates of dedifferentiation and for unambiguously labeling dedifferentiated GSCs.作者: 冒失 時間: 2025-3-28 10:02 作者: corporate 時間: 2025-3-28 11:05
Spermatogonial Dedifferentiation into Germline Stem Cells in , Testes, sibling GSCs. To facilitate the study of the cellular and molecular mechanisms underlying the process of germline dedifferentiation in the . testis, here we describe techniques for inducing high rates of dedifferentiation and for unambiguously labeling dedifferentiated GSCs.作者: BALK 時間: 2025-3-28 15:48
Identification of Germline Stem Cells in Zebrafish,ethod is presented here for identifying germline stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect . mRNA and immunofluorescence to detect the pan-germ cell marker Vasa.作者: 最高峰 時間: 2025-3-28 20:10
Maintenance of Human Primordial Germ Cell-Like Cells in a Long-Term Culture System,vitro models of gametogenesis have allowed the field to generate human primordial germ cell-like cells (hPGCLCs). In this chapter, we will review the generation of hPGCLCs using the incipient mesoderm-like cell (iMeLC) protocol and the subsequent expansion of hPGCLCs in a long-term culture system.作者: Pastry 時間: 2025-3-29 02:09 作者: Surgeon 時間: 2025-3-29 05:35
https://doi.org/10.1007/978-3-658-27172-5at, in the naked mole-rat (.) ovary, there is mitotic expansion of the PGCs, and the initiation of the meiotic program occurs postnatally. In this chapter, we present a comprehensive collection of protocols that permit the analysis of naked mole-rat germ cells, from PGCs to oocytes, in meiotic prophase I, using in vivo and in vitro approaches.作者: 異端邪說下 時間: 2025-3-29 10:14
David Jou,José Casas-Vázquez,Georgy Lebone for the isolation or enrichment of primordial germ cells (from 9.5?days . (.) to 18.5 .) by flow cytometry (Subheading .) or magnetic sorting (Subheading .), followed by feeder-free primary germ cell culture (Subheading .).作者: 波動 時間: 2025-3-29 14:44 作者: dissent 時間: 2025-3-29 17:07 作者: ethnology 時間: 2025-3-29 22:35
Isolation and Culture Techniques for Fetal Mouse Germ Cells,e for the isolation or enrichment of primordial germ cells (from 9.5?days . (.) to 18.5 .) by flow cytometry (Subheading .) or magnetic sorting (Subheading .), followed by feeder-free primary germ cell culture (Subheading .).作者: 雜色 時間: 2025-3-30 03:35
Analysis of the , Germline Stem Cell Pool,cell state to a differentiated state. The germline tissue is organized in an assembly line with the germline stem cell (GSC) pool at one end and differentiated gametes at the other. A simple mesenchymal niche caps the GSC pool and maintains GSCs in an undifferentiated state by signaling through the 作者: 不感興趣 時間: 2025-3-30 05:50 作者: TEN 時間: 2025-3-30 11:35 作者: 浮夸 時間: 2025-3-30 15:16 作者: Hyperplasia 時間: 2025-3-30 16:39
Targeting Endogenous Loci That Function in , Germline Stem Cells,e other genetic manipulations is useful for studying germline stem cell biology. In this chapter, we will describe a method to use CRISPR-Cas9 genome editing technology to make knock-out and knock-in flies. We will cover everything from guideRNA (gRNA) and donor plasmid design and cloning to screeni作者: Choreography 時間: 2025-3-31 00:35 作者: Genetics 時間: 2025-3-31 04:00 作者: Exposure 時間: 2025-3-31 08:41
Spermatogonial Dedifferentiation into Germline Stem Cells in , Testes,on as a conserved mechanism for replenishing stem cell pools after their loss, thereby maintaining tissue homeostasis. The testis of the fruit fly . offers a simple but powerful system to study dedifferentiation, the process by which differentiating spermatogonia can revert their fate to become full作者: 雄偉 時間: 2025-3-31 12:42 作者: 施舍 時間: 2025-3-31 14:11 作者: 1FAWN 時間: 2025-3-31 18:16 作者: Heretical 時間: 2025-4-1 00:43
A Roadmap for Three-Dimensional Analysis of the Intact Mouse Ovary,n of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analysis of whole ovarian tissue in 3D throughout murine development and aging.
