標題: Titlebook: Germline Development in the Zebrafish; Methods and Protocol Roland Dosch Book 2021 Springer Science+Business Media, LLC, part of Springer N [打印本頁] 作者: Stenosis 時間: 2025-3-21 16:07
書目名稱Germline Development in the Zebrafish影響因子(影響力)
書目名稱Germline Development in the Zebrafish影響因子(影響力)學科排名
書目名稱Germline Development in the Zebrafish網(wǎng)絡公開度
書目名稱Germline Development in the Zebrafish網(wǎng)絡公開度學科排名
書目名稱Germline Development in the Zebrafish被引頻次
書目名稱Germline Development in the Zebrafish被引頻次學科排名
書目名稱Germline Development in the Zebrafish年度引用
書目名稱Germline Development in the Zebrafish年度引用學科排名
書目名稱Germline Development in the Zebrafish讀者反饋
書目名稱Germline Development in the Zebrafish讀者反饋學科排名
作者: 沙發(fā) 時間: 2025-3-21 21:17 作者: 地殼 時間: 2025-3-22 02:47
,Die wichtigsten Fachausdrücke des Verkehrs, in early prophase for the first time in zebrafish oocytes, in four dimensions and in vivo. Our protocol expands the use of the zebrafish as a model system to understand germ cell and ovarian development in postembryonic stages.作者: Override 時間: 2025-3-22 07:30
Alternative Finanzierungsformen,ofluorescence, and fluorescent in situ hybridization with phalloidin labeling of F-actin) are intended for optimal preservation and visualization of both the cytoskeleton and RNAs of interest. These methods can also be modified and applied to a broad range of other uses.作者: 編輯才信任 時間: 2025-3-22 09:08 作者: anarchist 時間: 2025-3-22 13:14
Live and Time-Lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenil in early prophase for the first time in zebrafish oocytes, in four dimensions and in vivo. Our protocol expands the use of the zebrafish as a model system to understand germ cell and ovarian development in postembryonic stages.作者: anarchist 時間: 2025-3-22 20:17
Methods for Visualization of RNA and Cytoskeletal Elements in the Early Zebrafish Embryo,ofluorescence, and fluorescent in situ hybridization with phalloidin labeling of F-actin) are intended for optimal preservation and visualization of both the cytoskeleton and RNAs of interest. These methods can also be modified and applied to a broad range of other uses.作者: 小溪 時間: 2025-3-22 21:35 作者: 有毒 時間: 2025-3-23 04:54
1064-3745 ation advice from the experts.This volume details a wide range of methods, ranging from beginner through advanced, used to..further study zebrafish and fish germline.. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of t作者: TEN 時間: 2025-3-23 07:10
The export and shipping office, potential application in cryopreservation and surrogate production. However, it was assumed that the induction of fish PGCs in vitro is not possible because in vivo they inherit germ plasm. In this chapter, we describe a successful differentiation protocol explaining the crucial factors and steps for in vitro PGC generation.作者: CIS 時間: 2025-3-23 13:16
The Readymade Garment Sector in Pakistanical inhibition of RhoA and his effector ROCK affected the correct assembly of microtubules in the cleavage furrow with the concomitant abnormal localization of germ plasm mRNAs. The inhibition of RhoA/ROCK pathway caused a significant decrease in the germ cell population later in development.作者: macabre 時間: 2025-3-23 17:03
https://doi.org/10.1007/978-3-658-26046-0blation as a useful tool to probe tissue tension within the granulosa layer, an epithelial monolayer of somatic cells that surround the zebrafish female gamete during folliculogenesis. We describe in detail how to isolate follicles, mount samples, perform laser surgery, and analyze the data.作者: 警告 時間: 2025-3-23 19:45 作者: 讓你明白 時間: 2025-3-23 22:43
Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization,ical inhibition of RhoA and his effector ROCK affected the correct assembly of microtubules in the cleavage furrow with the concomitant abnormal localization of germ plasm mRNAs. The inhibition of RhoA/ROCK pathway caused a significant decrease in the germ cell population later in development.作者: tinnitus 時間: 2025-3-24 04:09
Quantifying Tissue Tension in the Granulosa Layer After Laser Surgery,blation as a useful tool to probe tissue tension within the granulosa layer, an epithelial monolayer of somatic cells that surround the zebrafish female gamete during folliculogenesis. We describe in detail how to isolate follicles, mount samples, perform laser surgery, and analyze the data.作者: 調(diào)色板 時間: 2025-3-24 09:38 作者: 階層 時間: 2025-3-24 11:24
Export beruflicher Aus- und Weiterbildungs, and can prevent catastrophic loss of irreplaceable research lines. In this chapter, we present the sperm collection, dilution, cryopreservation, thawing, and in vitro fertilization procedures used at the Zebrafish International Resource Center (ZIRC).作者: AROMA 時間: 2025-3-24 16:34
https://doi.org/10.1007/978-3-322-82854-5 first reported MC marker. Here, we describe a method to identify and mark the micropylar cell following the immunostaining procedure on cryosections or combining it with the RNA in situ hybridization on whole-mount follicles.作者: Callus 時間: 2025-3-24 22:18 作者: CHAFE 時間: 2025-3-25 02:46
Zum Begriff der Exportgemeinschaften,t of effective antibodies for these species. Here, we compare a protocol for zebrafish staining using glyoxal as a fixative agent with PFA. We demonstrate that glyoxal fixation improves the antigenicity of some epitopes thereby increasing the number of useful antibodies in zebrafish.作者: Expurgate 時間: 2025-3-25 03:45 作者: Mitigate 時間: 2025-3-25 07:50
Cryopreservation of Pooled Sperm Samples,s, and can prevent catastrophic loss of irreplaceable research lines. In this chapter, we present the sperm collection, dilution, cryopreservation, thawing, and in vitro fertilization procedures used at the Zebrafish International Resource Center (ZIRC).作者: 高原 時間: 2025-3-25 11:49 作者: 結(jié)構(gòu) 時間: 2025-3-25 18:11
Experimental Methodologies to Visualize Early Cell Biology in Zebrafish Embryos,ry, respectively. If performed correctly, these techniques can provide detailed information regarding the endogenous locations of proteins and their ectopic locations or absence in mutants and in disease states.作者: paroxysm 時間: 2025-3-25 20:53 作者: hemoglobin 時間: 2025-3-26 01:30 作者: 符合國情 時間: 2025-3-26 07:37
Vertiefung des Wissens in der Ausstellung, in aquaporin biology. This chapter focuses on techniques used for oocyte preparation and aquaporin expression and gives an overview of specific methods to determine water and solute permeability of the channels and their intracellular trafficking in oocytes.作者: 認識 時間: 2025-3-26 12:19
Export Consortia in Developing Countriesescribe essential steps needed for the cryopreservation of spermatogonial stem cells (SSCs) and their utilization in the conservation of zebrafish genetic resources through SSC transplantation and surrogate production.作者: Intuitive 時間: 2025-3-26 14:30 作者: oxidize 時間: 2025-3-26 19:00 作者: adj憂郁的 時間: 2025-3-26 23:13 作者: cardiac-arrest 時間: 2025-3-27 01:31 作者: pantomime 時間: 2025-3-27 05:41 作者: 是貪求 時間: 2025-3-27 11:57 作者: consent 時間: 2025-3-27 14:28 作者: 打火石 時間: 2025-3-27 19:29
Observation of Medaka Larval Gonads by Immunohistochemistry and Confocal Laser Microscopy,ning the gonad just beneath the coverslips is essential to obtain high-resolution images at a level where cellular components of germ cells, such as germ plasm and nuclear structures, can be clearly observed using an oil immersion objective lens.作者: 全部逛商店 時間: 2025-3-28 00:00
Explosive Welding, Forming and Compactionay to reveal the expression of individual genes in the two compartments of the follicle. This chapter introduces a method for mechanic separation of the oocyte and follicle layer at full-grown stage for expression analysis. Since fish have similar follicle structure, this method may also be used in other species as well.作者: Alpha-Cells 時間: 2025-3-28 05:39
Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish,ay to reveal the expression of individual genes in the two compartments of the follicle. This chapter introduces a method for mechanic separation of the oocyte and follicle layer at full-grown stage for expression analysis. Since fish have similar follicle structure, this method may also be used in other species as well.作者: 無底 時間: 2025-3-28 06:38
Roland DoschIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Congestion 時間: 2025-3-28 11:22 作者: Chronological 時間: 2025-3-28 16:18 作者: 小母馬 時間: 2025-3-28 19:50
Germline Development in the Zebrafish978-1-0716-0970-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 羞辱 時間: 2025-3-29 02:40
Mathematics and Its Applicationss an in vitro toxicology test system. One of the most sensitive and easily detectable parameters is progressive motility which can be measured by a computer-assisted sperm analysis (CASA) system. Here we describe a simple protocol to test the effect of environmental toxicants by using zebrafish (.) sperm.作者: 天賦 時間: 2025-3-29 04:25 作者: 衍生 時間: 2025-3-29 07:15
Computer-Assisted Sperm Analysis to Test Environmental Toxicants,s an in vitro toxicology test system. One of the most sensitive and easily detectable parameters is progressive motility which can be measured by a computer-assisted sperm analysis (CASA) system. Here we describe a simple protocol to test the effect of environmental toxicants by using zebrafish (.) sperm.作者: 合乎習俗 時間: 2025-3-29 13:06 作者: grenade 時間: 2025-3-29 17:55 作者: defenses 時間: 2025-3-29 23:21 作者: 使糾纏 時間: 2025-3-30 02:52
Mathematics and Its Applicationss an in vitro toxicology test system. One of the most sensitive and easily detectable parameters is progressive motility which can be measured by a computer-assisted sperm analysis (CASA) system. Here we describe a simple protocol to test the effect of environmental toxicants by using zebrafish (.) 作者: Transfusion 時間: 2025-3-30 07:32
Export Consortia in Developing Countriesic resources. However, currently, only spermatozoa of zebrafish can be successfully cryopreserved, while protocols for cryopreservation of eggs and embryos have not yet been fully developed. Transplantation of germline stem cells (GSCs) has risen as a favorable method that can bypass the current pro作者: 泥瓦匠 時間: 2025-3-30 08:50
Export Credit Insurance and Guaranteesng of this complicated process is utilization of severely sex-biased families or groups. Here, we describe a method for partial depletion of primordial germ cells (PGCs) that leads to eventual masculinization of zebrafish. The technique is based on injecting early embryos with diluted morpholino oli作者: AVOID 時間: 2025-3-30 13:43 作者: 制造 時間: 2025-3-30 19:50
The export and shipping office,re maternal and paternal genomes until embryo cryopreservation is achieved. However, the number of these cells in an embryo is very low limiting their potential application in cryopreservation and surrogate production. However, it was assumed that the induction of fish PGCs in vitro is not possible 作者: avarice 時間: 2025-3-31 00:01 作者: ANN 時間: 2025-3-31 01:22
Export beruflicher Aus- und Weiterbildungrvation minimizes expenses for animal and facility maintenance such as personnel, water, power, and space. It extends the time offspring can be produced from individual organisms, reduces the need to maintain live populations, provides flexibility for planning future experiments and research project作者: etidronate 時間: 2025-3-31 08:54 作者: 妨礙 時間: 2025-3-31 12:49
,Einführung in die Problemstellung, the same individual over time. Optical-based imaging methods can hardly be applied on the adult zebrafish, due to their limited transparency. In this chapter, we will demonstrate the application of micro?computer tomography (CT) imaging for in vivo 3D imaging of the gonads in adult zebrafish. We ex作者: somnambulism 時間: 2025-3-31 15:28
,Die wichtigsten Fachausdrücke des Verkehrs,s of oogenesis involve the coordination of simultaneous and tightly sequential processes across cellular compartments and between sister cells. To fully understand the mechanistic framework of these coordinated processes, cellular and morphological analysis in high temporal resolution is required. H作者: Nonflammable 時間: 2025-3-31 18:40 作者: Brittle 時間: 2025-3-31 22:08
https://doi.org/10.1007/978-3-322-82854-5specialized follicle cell, called the micropylar cell (MC). Very little is known about the mechanisms underlying the specification and differentiation of the MC, a unique cell among hundreds that compose the follicle cell layer. The Hippo pathway effector Taz is essential for this process and is the
