作者: 歡呼 時(shí)間: 2025-3-21 21:48
Eurodollars and International Banking reporter gene to tag the gene either in a construct (transgenesis) or in the genome (homologous recombination). Transgenes have been generated using different regions of the . gene promoter to drive expression of either lacZ or GFP, which allows the activity of the . promoter to be followed in livi作者: aphasia 時(shí)間: 2025-3-22 04:27
https://doi.org/10.1007/978-3-642-69906-1). It is used to study individual genes, gene families, and also for genomewide screenings (.). In a RNAi experiment . is exposed to double-stranded RNA that corresponds to the sequence of a specific mRNA. This triggers a series of enzymatic processes, resulting in a specific degradation of this tar作者: Aqueous-Humor 時(shí)間: 2025-3-22 06:57 作者: 籠子 時(shí)間: 2025-3-22 08:49 作者: NADIR 時(shí)間: 2025-3-22 16:05 作者: NADIR 時(shí)間: 2025-3-22 20:06 作者: 天氣 時(shí)間: 2025-3-22 22:49
https://doi.org/10.1007/978-3-322-89332-1minate between multiple fluorophores. Recently developed techniques, such as confocal (.) or multiphoton imaging (.), permit optical sectioning of intact specimens. These may be collected as stacks of images at different focal depths to obtain three-dimensional (3D) structural data. Stacks of images作者: Physiatrist 時(shí)間: 2025-3-23 03:59 作者: 思想靈活 時(shí)間: 2025-3-23 08:06
Hans J. Kleinsteuber,Torsten Rossmanniculture, biomedicine, and basic research. Based on the source of donor cells, NT can be classified into embryonic cell NT and somatic cell NT. Somatic cell NT was first reported in 1996 (.) and includes more practical applications. Most importantly, it provides a promising method for producing tran作者: LATER 時(shí)間: 2025-3-23 13:37
https://doi.org/10.1007/978-3-322-86885-5mouse is genetically the most thoroughly analyzed species. Hundreds of different mutant mouse strains are being bred in laboratories and stored as frozen embryos or germ cells in repositories around the world. Because spontaneous mutations are very rare events, different methods to increase the inci作者: Common-Migraine 時(shí)間: 2025-3-23 17:35 作者: Prostatism 時(shí)間: 2025-3-23 20:29
,Die Europ?ischen Organisationen,ryos (.); however, with this technique, only a few animals of the same genetic origin could be produced (.). The second approach, which seemed to be more promising, was the embryo reconstruction using a single nucleus from an embryo to an enucleated one-cell embryo. This technique was based on an id作者: 察覺 時(shí)間: 2025-3-23 23:28
,Nordeuropa im frühen Mittelalter 400–900, most similarities to that of the human (.). Biomedical studies in the domestic cat have contributed significantly to our knowledge in the areas of immunology, infectious diseases, genetics, neurophysiology, and cancer (.). Many of the heritable disorders of cats are analogous to those of humans, in作者: dandruff 時(shí)間: 2025-3-24 05:35
Wirtschaftspolitische Schlussfolgerungen,recipient embryos and chickens, or analyzing the developmental fate of transferred donor cells into recipients. Production of germline chimeric chickens makes it possible to manipulate germline cells in vitro (e.g., DNA transfer or cryopreservation), and the manipulated germline cells can allow viab作者: 條街道往前推 時(shí)間: 2025-3-24 08:55
Florian Baumann,Sebastian Sch?fferponents required for the initiation of development. Through embryonic cloning scenarios, it is now clear that such cytoplasm is a primary requirement—necessary and sufficient (with some limitations) for supporting term development even through the control of a previously quiescent somatic genome (.,作者: GENRE 時(shí)間: 2025-3-24 11:07 作者: 的染料 時(shí)間: 2025-3-24 16:52
Parthenogenesis and Nuclear Transfer in Rabbit Oocytes,ound are difficult to obtain, as few inbred strains have been established because of the fact that inbreeding decreases reproductive ability. Moreover, an embryonic stem (ES) cell line with germline chimeras has not been established in rabbits; and thus, there are no reports on production of transgenic rabbits by gene targeting.作者: sebaceous-gland 時(shí)間: 2025-3-24 19:43
Heide SchattenIncludes supplementary material: 作者: 名字的誤用 時(shí)間: 2025-3-25 01:31
Production of Haploid and Diploid Androgenetic Zebrafish,作者: 折磨 時(shí)間: 2025-3-25 04:32
Construction and Detection of Fluorescent, Germline Transgenic Zebrafish,作者: 面包屑 時(shí)間: 2025-3-25 07:37
Production of Zebrafish Germline Chimeras from Cultured Cells,作者: 笨拙的我 時(shí)間: 2025-3-25 15:13
https://doi.org/10.1057/9780230377554he tester population that remain single-stranded are further amplified, thus representing the pool of differentially expressed genes. This pool enriched for differentially expressed sequences can easily be cloned to generate a subtraction library. The analysis of subtraction efficiency is a crucial 作者: HATCH 時(shí)間: 2025-3-25 16:55
https://doi.org/10.1007/978-3-663-13434-3lular basis for embryonic defects or the interpretation of knockout phenotypes, especially those complicated by early embryonic lethality. In addition, great efforts are made to define protocols to preferentially direct ES cell differentiation as EBs toward one single cell fate, therefore providing 作者: Between 時(shí)間: 2025-3-25 20:48
Hans J. Kleinsteuber,Torsten Rossmann.). However, when measured as development to term as a proportion of oocytes used, the efficiency of somatic cell NT, has been very low (1–2%). Several variables influence the ability to reproduce a specific genotype by cloning. These include species, source of recipient ova, cell type of nuclei don作者: AGONY 時(shí)間: 2025-3-26 03:46
https://doi.org/10.1007/978-3-322-86885-5genome with the objective of creating transgenic mice. In contrast to chemical mutagenesis, where phenotypic changes are a prerequisite to identify the induced mutation (forward genetics), transgenic technology is based on the reintroduction of a previously isolated and in vitro recombined DNA seque作者: debase 時(shí)間: 2025-3-26 07:25
,Die Europ?ischen Organisationen,ent parts of the genetic information, not because the total amount of information differs. Furthermore, all these cells have genetic information present in the one-cell embryo that develop into the animal. Therefore, if the nucleus of any of these cells were used to replace the genetic information i作者: Mnemonics 時(shí)間: 2025-3-26 11:38
Florian Baumann,Sebastian Sch?ffer understanding of basic developmental biology, but also has critical implications in animal husbandry and biotechnology (.), as well as in assisted reproductive medicine (.). Experimental manipulation of the cytoplasm of oocytes and early embryos has been a significant component of this research. Al作者: labile 時(shí)間: 2025-3-26 15:43 作者: CYT 時(shí)間: 2025-3-26 17:20 作者: Emasculate 時(shí)間: 2025-3-27 00:55
Differentiating Embryonic Stem Cells into Embryoid Bodies,lular basis for embryonic defects or the interpretation of knockout phenotypes, especially those complicated by early embryonic lethality. In addition, great efforts are made to define protocols to preferentially direct ES cell differentiation as EBs toward one single cell fate, therefore providing 作者: 我悲傷 時(shí)間: 2025-3-27 05:09 作者: liposuction 時(shí)間: 2025-3-27 08:45
Pronuclear Microinjection of Mouse Zygotes,genome with the objective of creating transgenic mice. In contrast to chemical mutagenesis, where phenotypic changes are a prerequisite to identify the induced mutation (forward genetics), transgenic technology is based on the reintroduction of a previously isolated and in vitro recombined DNA seque作者: 分解 時(shí)間: 2025-3-27 10:41 作者: BOAST 時(shí)間: 2025-3-27 16:12
Cytoplasmic Transfer in Mammalian Eggs and Embryos, understanding of basic developmental biology, but also has critical implications in animal husbandry and biotechnology (.), as well as in assisted reproductive medicine (.). Experimental manipulation of the cytoplasm of oocytes and early embryos has been a significant component of this research. Al作者: 最高峰 時(shí)間: 2025-3-27 20:15
Book 2004xplored in sea urchin and asc- ian eggs, now benefits cloning efficiency in farm and domestic animals. Gene manipulations and molecular methods have added to the possibilities of p- ducing live offspring with enormous biomedical, ecological, and economic implications. All sexually reproducing organi作者: coltish 時(shí)間: 2025-3-28 01:24 作者: 抗體 時(shí)間: 2025-3-28 06:10
1064-3745 hase of new discoveries with the introd- tion of transgenic technologies and nuclear cloning. Basic knowledge and te- niques developed for lower vertebrate and invertebrate systems have facilitated the study of higher vertebrates, including humans. Many experiments that have first been performed on 作者: 推崇 時(shí)間: 2025-3-28 08:40
Wirtschaftspolitische Schlussfolgerungen,ns makes it possible to manipulate germline cells in vitro (e.g., DNA transfer or cryopreservation), and the manipulated germline cells can allow viable offspring by germline chimeric chickens. It is expected that endangered avian species can also proliferate via interspecific germline chimeric chickens.作者: mortgage 時(shí)間: 2025-3-28 12:53 作者: 從屬 時(shí)間: 2025-3-28 14:37 作者: 華而不實(shí) 時(shí)間: 2025-3-28 19:57 作者: 使長(zhǎng)胖 時(shí)間: 2025-3-29 00:30 作者: FADE 時(shí)間: 2025-3-29 03:51 作者: Console 時(shí)間: 2025-3-29 11:07 作者: 相符 時(shí)間: 2025-3-29 11:55 作者: NIL 時(shí)間: 2025-3-29 19:10
Dual-Channel Confocal Ratioing of Calcium Dynamics in Living Eggs and Oocytes,ction with fluorescence microscopy to track calcium dynamics during fertilization. The relative benefits and disadvantages of aequorin vs calcium-sensitive probes have been discussed previously (.), and both types of calcium indicators remain widely employed in imaging analyses that involves conventional microscopy.作者: Condyle 時(shí)間: 2025-3-29 23:33
Book 2004on of transgenic technologies and nuclear cloning. Basic knowledge and te- niques developed for lower vertebrate and invertebrate systems have facilitated the study of higher vertebrates, including humans. Many experiments that have first been performed on lower vertebrates provided the tools and st作者: 發(fā)酵 時(shí)間: 2025-3-30 03:49
https://doi.org/10.1007/978-3-642-69906-1get mRNA (.–7). Consequently the animals display a phenotype that results from the depletion of the target protein. The RNAi phenotype can reach the severity of a null mutant (.). Although, generally RNAi is far more effective than antisense RNA (.,.) the effectiveness of a specific RNAi experiment depends on the target gene.作者: Tortuous 時(shí)間: 2025-3-30 04:44 作者: –DOX 時(shí)間: 2025-3-30 11:12 作者: 致敬 時(shí)間: 2025-3-30 16:03 作者: Ancestor 時(shí)間: 2025-3-30 16:54
Activity of the Germline-Specific ,-GFP Transgene in Normal and Clone Mouse Embryos,toside) to produce a blue color reaction. The green fluorescent protein (GFP) from the jellyfish . gives a natural green fluorescence that can be viewed under blue light excitation without processing the biological substrate for any chemical/enzymatic reaction. GFP is a convenient reporter for several reasons:作者: xanthelasma 時(shí)間: 2025-3-30 22:39
In Vitro Fertilization and Embryo Transfer in Felids,omyelinosis C. The close phylogenetic relationship, and the fact that many analogous genetic disorders have been identified and characterized, are important factors that demonstrate the advantages of domestic cats as biomedical research models for human disease when compared to other laboratory animals.作者: CAPE 時(shí)間: 2025-3-31 02:47
Activity of the Germline-Specific ,-GFP Transgene in Normal and Clone Mouse Embryos, reporter gene to tag the gene either in a construct (transgenesis) or in the genome (homologous recombination). Transgenes have been generated using different regions of the . gene promoter to drive expression of either lacZ or GFP, which allows the activity of the . promoter to be followed in livi作者: Osmosis 時(shí)間: 2025-3-31 08:08 作者: 顯示 時(shí)間: 2025-3-31 11:25 作者: Enliven 時(shí)間: 2025-3-31 13:22 作者: Brochure 時(shí)間: 2025-3-31 21:13
Differentiating Embryonic Stem Cells into Embryoid Bodies,tained in a pluripotent state for indefinite periods of time in the presence of the leukemia inhibitory factor (LIF) or in coculture with mouse embryonic fibroblasts (MEFs). When cultured in the absence of LIF, ES cells differentiate spontaneously, forming three-dimensional (3D) aggregates called . 作者: 黑豹 時(shí)間: 2025-3-31 21:43 作者: CHASM 時(shí)間: 2025-4-1 04:49
Using Multiphoton Excitation to Explore the Murky Depths of Developing Embryos,minate between multiple fluorophores. Recently developed techniques, such as confocal (.) or multiphoton imaging (.), permit optical sectioning of intact specimens. These may be collected as stacks of images at different focal depths to obtain three-dimensional (3D) structural data. Stacks of images作者: 改良 時(shí)間: 2025-4-1 08:02 作者: 反應(yīng) 時(shí)間: 2025-4-1 10:53
A Method for Producing Cloned Pigs by Using Somatic Cells as Donors,iculture, biomedicine, and basic research. Based on the source of donor cells, NT can be classified into embryonic cell NT and somatic cell NT. Somatic cell NT was first reported in 1996 (.) and includes more practical applications. Most importantly, it provides a promising method for producing tran作者: 責(zé)難 時(shí)間: 2025-4-1 14:39
