標(biāo)題: Titlebook: Germ Cell Development; Methods and Protocol Marco Barchi,Massimo De Felici Book 2024 The Editor(s) (if applicable) and The Author(s), under [打印本頁(yè)] 作者: Abridge 時(shí)間: 2025-3-21 16:13
書目名稱Germ Cell Development影響因子(影響力)
作者: confederacy 時(shí)間: 2025-3-21 20:30 作者: Gene408 時(shí)間: 2025-3-22 01:07
Germ Cell Development978-1-0716-3698-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: saturated-fat 時(shí)間: 2025-3-22 07:56
Demeter and Kore in Mississippi,c information to subsequent generations. Studies aimed to unravel PGC development at molecular level in mammals can be traced at the early 1980s and were hampered by the difficulty in obtaining both sufficient quantities and purity of PGCs. For many laboratories, the isolation and purification metho作者: Affable 時(shí)間: 2025-3-22 11:46
Emp?rung, Wahrhaftigkeit und Common Sensepassed to the next generations. PGCs are found in different locations and variable numbers in the chick embryo, as in other species, depending on the developmental stages. Here, we describe in detail a method based on the Percoll gradient, routinely used in our laboratory, allowing us to obtain from作者: 瘙癢 時(shí)間: 2025-3-22 13:00 作者: 瘙癢 時(shí)間: 2025-3-22 17:57 作者: 你正派 時(shí)間: 2025-3-23 00:42
https://doi.org/10.1007/978-1-349-16913-9s been used for many years to sub-divide these cells based on their DNA content. This approach provides an efficient way to obtain broad categories of male germ cells: pre-meiotic spermatogonia, meiotic spermatocytes and post-meiotic spermatids. The addition of a red filter for Hoechst staining enab作者: 發(fā)電機(jī) 時(shí)間: 2025-3-23 01:59 作者: FLOAT 時(shí)間: 2025-3-23 08:30 作者: 或者發(fā)神韻 時(shí)間: 2025-3-23 12:49
Eugène Dubois and the Ape-Man from Javading the fundamentals of cell fate decisions, animal breeding, and future reproductive medicine. Here, we introduce this system established in the rat model. We describe a stepwise protocol to induce epiblast-like cells and subsequent PGCLCs by forming spherical aggregates from rat PSCs. We also des作者: JEER 時(shí)間: 2025-3-23 15:54 作者: Chronological 時(shí)間: 2025-3-23 20:04
,Eugène Labiclie and the Second Empire, that CRISPR/Cas9 alters the genome of cultured male mouse germline stem cells delivered by electroporation of plasmids. Recently, we showed proof-of-principle that gene knockout can be effectively targeted in mouse germline stem cells by lipofecting Cas9:gRNA ribonucleoproteins. In this protocol, w作者: escalate 時(shí)間: 2025-3-23 23:14
https://doi.org/10.1007/978-3-030-44826-4ls differentiate, surrounded by a basement membrane and interstitium. 3D bioprinting might be used to recreate the compartmentalized testicular architecture in vitro. Directed by a software program, pneumatic microextrusion printers can deposit 3D layers of hydrogel-encapsulated interstitial cells i作者: trigger 時(shí)間: 2025-3-24 04:36
Binding of Ligands to Receptors: Theory,specific approaches when studying mammalian female fertility to preserve ovary and oocyte structures effectively. The methods of collecting and culturing ovaries and oocytes play an essential role in the study of mammalian follicle development and oocyte quality. This chapter presents a collection o作者: 錫箔紙 時(shí)間: 2025-3-24 08:14
The Production of Transgenic Mice,tory ones. Primordial follicles constitute the most abundant source of gametes inside the mammalian ovary at any given time..The isolation of a high number of primordial follicles, together with the development of protocols for in vitro follicle growth, would provide a powerful tool to fully exploit作者: 招人嫉妒 時(shí)間: 2025-3-24 11:33
Alexandria, das Museion, Euklid,limited source of patient-specific cells that can be virtually differentiated in any cell of the human body. The generation of iPSCs has important implications for all human medicine fields, as they can be used for drug discovery, regenerative medicine, and developmental studies. Klinefelter Syndrom作者: arthroscopy 時(shí)間: 2025-3-24 16:43
https://doi.org/10.1007/978-3-030-49402-5 the transcriptomes, that is of the RNA transcripts produced by the genotype at a given time, of germ cells and the surrounding somatic cells, is essential to unravel the cellular and molecular processes regulating gametogenesis. However, the asynchronized differentiation of germ cells and high cell作者: 全神貫注于 時(shí)間: 2025-3-24 20:32
Marco Barchi,Massimo De FeliciIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: Graduated 時(shí)間: 2025-3-25 00:44 作者: 打擊 時(shí)間: 2025-3-25 05:04 作者: expire 時(shí)間: 2025-3-25 09:29
Book 2024hods and Protocols. provides new insight into the understanding of basic biological aspects of germ cell biology, as well as the opportunity for in vitro manipulation of germ cells for toxicology studies and for genetic intervention..作者: 躲債 時(shí)間: 2025-3-25 13:13
,Rethinking O’Neill’S Beginnings,acterization of the different testicular cell populations to investigate the transition between FGCs and spermatogonia and shed some light on crucial processes of early human gametogenesis unknown until now.作者: 神圣在玷污 時(shí)間: 2025-3-25 17:01 作者: Subjugate 時(shí)間: 2025-3-25 23:22 作者: defeatist 時(shí)間: 2025-3-26 02:35
Isolation and In Vitro Culture of Germ Cells and Sertoli Cells from Human Fetal Testisacterization of the different testicular cell populations to investigate the transition between FGCs and spermatogonia and shed some light on crucial processes of early human gametogenesis unknown until now.作者: 影響深遠(yuǎn) 時(shí)間: 2025-3-26 07:20
Human Primordial Germ Cell-Like Cell Induction from Pluripotent Stem Cells by SOX17 and PRDM1 Expresedure for PGCLC differentiation with the balanced induction of SOX17 and PRDM1. The protocol can be used for PGC induction in other mammalian species exhibiting PGCs with SOX17 expression. Together, these studies will advance the understanding of germ cell biology and its applications in reproductiv作者: expdient 時(shí)間: 2025-3-26 08:59
Data Analysis Pipeline for scRNA-seq Experiments to Study Early Oogenesisrmation. Here, using the 10× Genomic platform and the most widely cited bioinformatics tools, we describe how to analyze early female germ cell development using scRNA-seq data generated from mouse E11.5 to E14.5 ovaries. This pipeline will provide a guide for exploring the processes of early germ c作者: expunge 時(shí)間: 2025-3-26 15:56
1064-3745 ent: Methods and Protocols. provides new insight into the understanding of basic biological aspects of germ cell biology, as well as the opportunity for in vitro manipulation of germ cells for toxicology studies and for genetic intervention..978-1-0716-3700-5978-1-0716-3698-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 沒花的是打擾 時(shí)間: 2025-3-26 17:20 作者: Commodious 時(shí)間: 2025-3-26 21:50 作者: 召集 時(shí)間: 2025-3-27 01:12 作者: Truculent 時(shí)間: 2025-3-27 05:23 作者: lethargy 時(shí)間: 2025-3-27 11:46
Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model homogeneous population of viable primordial follicles suitable for in vitro culture. Here, we provide a fast and high-yield strategy for the mechanical isolation of primordial follicles from limited portions of the ovarian cortex in the bovine animal model.作者: excrete 時(shí)間: 2025-3-27 15:41 作者: 乞丐 時(shí)間: 2025-3-27 20:43 作者: 北極熊 時(shí)間: 2025-3-28 00:47
1064-3745 expertsThis detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and i作者: Engaged 時(shí)間: 2025-3-28 04:11
Emp?rung, Wahrhaftigkeit und Common Sensedevelopmental stages. Here, we describe in detail a method based on the Percoll gradient, routinely used in our laboratory, allowing us to obtain from blood and gonad anlages significant numbers of viable PGCs which can be successfully cultured or efficiently genetically modified.作者: anagen 時(shí)間: 2025-3-28 08:35 作者: Congruous 時(shí)間: 2025-3-28 14:13
Eugène Dubois and the Ape-Man from Java model. We describe a stepwise protocol to induce epiblast-like cells and subsequent PGCLCs by forming spherical aggregates from rat PSCs. We also describe a protocol to mature these PGCLCs from specified/migratory to the gonadal stage by aggregation with female gonadal somatic cells.作者: Ingest 時(shí)間: 2025-3-28 17:04 作者: 斗爭(zhēng) 時(shí)間: 2025-3-28 22:02 作者: 語源學(xué) 時(shí)間: 2025-3-29 00:06
Demeter and Kore in Mississippi,ular levels. In the present chapter, I focus on immunomagnetic cell sorting (MACS) and fluorescence-activated cell sorting (FACS) methods used in my laboratory for the purification of mouse PGCs from 10.5 to 12.5?dpc embryos before their differentiation in oogonia/oocytes in female and prospermatogonia in male.作者: Infinitesimal 時(shí)間: 2025-3-29 03:14 作者: inculpate 時(shí)間: 2025-3-29 08:20
https://doi.org/10.1007/978-3-030-44826-4ells, the epithelial cell fraction can be seeded in the macropores to resemble the in vivo testicular architecture. Moreover, macropores can serve as a delimitation for all testicular cells to reorganize and improve the supply of nutrients to cells through the 3D constructs.作者: 不適當(dāng) 時(shí)間: 2025-3-29 13:49 作者: 軟膏 時(shí)間: 2025-3-29 17:41 作者: 尊重 時(shí)間: 2025-3-29 20:36 作者: 敬禮 時(shí)間: 2025-3-29 23:55
Purification and Transfection Methods of Chicken Primordial Germ Cellsdevelopmental stages. Here, we describe in detail a method based on the Percoll gradient, routinely used in our laboratory, allowing us to obtain from blood and gonad anlages significant numbers of viable PGCs which can be successfully cultured or efficiently genetically modified.作者: intimate 時(shí)間: 2025-3-30 05:21 作者: BOON 時(shí)間: 2025-3-30 10:55 作者: Axillary 時(shí)間: 2025-3-30 13:58
Lipofection-Based Delivery of CRISPR/Cas9 Ribonucleoprotein for Gene Editing in Male Germline Stem Cprinciple that gene knockout can be effectively targeted in mouse germline stem cells by lipofecting Cas9:gRNA ribonucleoproteins. In this protocol, we describe a simple, fast, and cheap workflow for gene editing via the lipofection of non-integrative ribonucleoproteins in murine male germline stem cells.作者: INCH 時(shí)間: 2025-3-30 18:03
Histological and Cytological Techniques to Study Perinatal Mouse Ovaries and Oocytesing ovaries and oocytes play an essential role in the study of mammalian follicle development and oocyte quality. This chapter presents a collection of protocols that focus on various methods for studying mammalian ovaries and oocytes, providing researchers with a variety of approaches to choose from.作者: Instantaneous 時(shí)間: 2025-3-30 22:31
Isolation and Purification of Viable PGCs from Mouse Embryosc information to subsequent generations. Studies aimed to unravel PGC development at molecular level in mammals can be traced at the early 1980s and were hampered by the difficulty in obtaining both sufficient quantities and purity of PGCs. For many laboratories, the isolation and purification metho
