標(biāo)題: Titlebook: Genomics Protocols; Michael P. Starkey,Ramnath Elaswarapu Book 20011st edition Springer Science+Business Media New York 2001 [打印本頁] 作者: NK871 時(shí)間: 2025-3-21 17:42
書目名稱Genomics Protocols影響因子(影響力)
書目名稱Genomics Protocols影響因子(影響力)學(xué)科排名
書目名稱Genomics Protocols網(wǎng)絡(luò)公開度
書目名稱Genomics Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Genomics Protocols被引頻次
書目名稱Genomics Protocols被引頻次學(xué)科排名
書目名稱Genomics Protocols年度引用
書目名稱Genomics Protocols年度引用學(xué)科排名
書目名稱Genomics Protocols讀者反饋
書目名稱Genomics Protocols讀者反饋學(xué)科排名
作者: Foam-Cells 時(shí)間: 2025-3-21 23:50 作者: 猛擊 時(shí)間: 2025-3-22 03:02
Sequence-Based Detection of Single Nucleotide Polymorphisms,resence in both coding and noncoding regions, single nucleotide substitutions are probably the underlying cause of most phenotypic differences among humans. Therefore, the identification of SNPs in human genes will play an increasingly important role in analyzing genotype-phenotype correlations with作者: GOAT 時(shí)間: 2025-3-22 04:46 作者: 帳單 時(shí)間: 2025-3-22 09:24
Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization,tasis. In addition, CGH can be used to compare different histologic components within one tumor, enabling a better understanding of the relation between phenotype and genotype, or to compare derivative cell lines with the original cell line.作者: Hiatal-Hernia 時(shí)間: 2025-3-22 15:30 作者: Hiatal-Hernia 時(shí)間: 2025-3-22 20:05
Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping,ms et al. (.) reported the generation of 174,172 human partial cDNA sequences and introduced the term . (ESTs). Since then, other major sequencing efforts (.) have brought the number of human ESTs in databases (.) to more than 1,400,000.作者: forecast 時(shí)間: 2025-3-23 00:51 作者: 消散 時(shí)間: 2025-3-23 01:51
Die Allgemeinbehandlung der Tuberkulose,resence in both coding and noncoding regions, single nucleotide substitutions are probably the underlying cause of most phenotypic differences among humans. Therefore, the identification of SNPs in human genes will play an increasingly important role in analyzing genotype-phenotype correlations with作者: 顛簸下上 時(shí)間: 2025-3-23 08:59
Diagnostik und Therapie der Pulpakrankheitens from the same individual) are removed. The . mismatch repair enzyme selection facilitates the removal of most of the mismatch-containing heterohybrids (.,.), therefore, DNA fragments from all IBD regions are isolated on the basis of their ability to form extended mismatch-free heterohybrids (doubl作者: FICE 時(shí)間: 2025-3-23 12:23 作者: 戲法 時(shí)間: 2025-3-23 17:40 作者: 平躺 時(shí)間: 2025-3-23 21:01 作者: Irksome 時(shí)間: 2025-3-24 01:55 作者: 宿醉 時(shí)間: 2025-3-24 05:53
Springer Science+Business Media New York 2001作者: 天氣 時(shí)間: 2025-3-24 08:26
Genomics Protocols978-1-59259-235-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 諷刺滑稽戲劇 時(shí)間: 2025-3-24 10:54 作者: 材料等 時(shí)間: 2025-3-24 18:23
https://doi.org/10.1007/978-3-642-73492-2Fluorescence . hybridization (FISH) provides a rapid means of placing labeled DNA segments into a wider genomic context. Mapping to banded metaphase chromosomes anchors clones for specific genes or markers in a well-recognized framework and provides a useful confirmation that clones belong to the expected region of interest.作者: Abnormal 時(shí)間: 2025-3-24 19:35 作者: Fortify 時(shí)間: 2025-3-25 02:55
https://doi.org/10.1007/978-3-642-56883-1 F factor allows strict copy number control of the clones so that they are stably maintained at one to two copies per cell (.). The stability of the cloned DNA during propagation in . host is substantially higher in lower copy number vectors than in multi-copy counterparts.作者: Anticonvulsants 時(shí)間: 2025-3-25 06:07
Aufrechterhaltung der Abstinenz is to generate fingerprints for all the clones in a given project. The overlap between clones is determined by the proportion of comigrating restriction fragments on either acrylamide or agarose gels, with mulitfold coverage of clones allowing rapid identification of artifacts such as rearranged or deleted clones.作者: 嫻熟 時(shí)間: 2025-3-25 09:06
https://doi.org/10.1007/978-3-642-91757-8 by many types of conventional methods have a high content of nonfull-length cDNA clones. One of the reasons for this is that reverse transcriptase (RT) tends to stop during the first strand synthesis and falls off, leaving nonfull-length cDNA. Thus, nonfull-length cDNA is an inevitable result of the use of RT for the synthesis of cDNA.作者: violate 時(shí)間: 2025-3-25 15:01
H. Mudra,W. Zwehl,V. Klauss,K. Theisenally expressed genes. RDA was originally developed for genomic DNA as a method to isolate the differences between two complex genomes (.) and later was adapted for cDNA to examine differential gene expression (.).作者: Migratory 時(shí)間: 2025-3-25 18:00 作者: GOAD 時(shí)間: 2025-3-25 22:54 作者: 不透明 時(shí)間: 2025-3-26 00:49
Construction of Full-Length-Enriched cDNA Libraries, by many types of conventional methods have a high content of nonfull-length cDNA clones. One of the reasons for this is that reverse transcriptase (RT) tends to stop during the first strand synthesis and falls off, leaving nonfull-length cDNA. Thus, nonfull-length cDNA is an inevitable result of the use of RT for the synthesis of cDNA.作者: HARP 時(shí)間: 2025-3-26 07:46 作者: 松軟無力 時(shí)間: 2025-3-26 10:03
Max Ragaller,Birgit Gottschlich locus in a genome scan, determination of the most likely map location for the gene, and finally fine mapping of the locus. A number of experimental strategies for mapping genes in experimental organisms are available and their relative merits have been reviewed recently (.). Whatever strategy is ch作者: Cuisine 時(shí)間: 2025-3-26 15:43
Die Kollapstherapie der Lungentuberkulose, in computer programs such as LINKAGE (.), has been enormously successful in facilitating identification of the genes responsible. These monogenic models typically use two alleles to represent the trait locus, one allele predisposing to development of the disease or disorder and the other allele sho作者: ALERT 時(shí)間: 2025-3-26 19:51 作者: ALERT 時(shí)間: 2025-3-26 21:09
Diagnostik und Therapie der Pulpakrankheitenilability of simple PCR techniques, vast numbers of highly informative genomewide polymorphic markers, and more recently, radiation hybrid mapping, DNA microarrays, and gene chip technology (Research Genetics, AL), GMS is a very practical shortcut to conventional genetic linkage methods. The basic p作者: Accord 時(shí)間: 2025-3-27 04:07
Sonstige diagnostische Verfahrentic analysis, it needs no cell culturing, making it applicable to practically any kind of clinical specimen from which DNA can be obtained, including archival paraffin-embedded material (.). CGH maps the origins of amplified and deleted DNA sequences on normal chromosomes, thereby highlighting locat作者: 舞蹈編排 時(shí)間: 2025-3-27 07:23
https://doi.org/10.1007/978-3-642-56883-1 F factor allows strict copy number control of the clones so that they are stably maintained at one to two copies per cell (.). The stability of the cloned DNA during propagation in . host is substantially higher in lower copy number vectors than in multi-copy counterparts.作者: Middle-Ear 時(shí)間: 2025-3-27 10:32 作者: 冒煙 時(shí)間: 2025-3-27 14:01
https://doi.org/10.1007/978-3-662-11471-1o position them on the corresponding integrated map, has long been a key issue in genetics. In all three fields of mapping, enormous progress has been made over the past two decades through either the development of new reagents or innovations in technology. The use of sequence tag sites (STSs) as m作者: committed 時(shí)間: 2025-3-27 18:38 作者: 膽小鬼 時(shí)間: 2025-3-27 22:28
https://doi.org/10.1007/978-3-662-68601-0h as mammals is not only owing to the large number of expected genes (70,000–100,000) in humans (.) but also because gene coding regions are dispersed in the genome. In addition, only a subset of all transcripts is found in a given cell type, typically in the range of 10,000, and the presence of cer作者: laceration 時(shí)間: 2025-3-28 02:32
Die Aufgaben des LeDi-Arithmetik,separate wells of microtiter plates. This not only makes clones practically immortal by keeping the plates at ?80°C, but it also gives each of the clones a unique reproducible identity, which allows scientists in different laboratories to be certain to use the same biological material for their vari作者: avulsion 時(shí)間: 2025-3-28 06:45 作者: 衍生 時(shí)間: 2025-3-28 14:04 作者: Delirium 時(shí)間: 2025-3-28 15:59 作者: output 時(shí)間: 2025-3-28 21:02 作者: Gene408 時(shí)間: 2025-3-29 02:49
https://doi.org/10.1007/978-3-642-67683-3r 3000 Mb of haploid DNA. Owing to the uniformity and simplicity of the DNA code, it is not an easy task to identify genes even after the region in which they lie has been fully sequenced. The average length of a human coding sequence in the DNA databases is approx 1.2 kb, and sensible estimates of 作者: 外來 時(shí)間: 2025-3-29 04:43 作者: 浮雕寶石 時(shí)間: 2025-3-29 09:16 作者: 陰謀 時(shí)間: 2025-3-29 14:14 作者: Fluctuate 時(shí)間: 2025-3-29 19:17
Peripheres cerebrospinales Nervensystem, directly from the clone under analysis without knowledge or availability of tissues expressing the gene to be identified. Furthermore, because isolation is not based on hybridization, it is not possible to isolate highly similar sequences that derive from other parts of the genome, not under analysis.作者: amphibian 時(shí)間: 2025-3-29 21:24 作者: kyphoplasty 時(shí)間: 2025-3-30 01:07 作者: 使無效 時(shí)間: 2025-3-30 05:19 作者: Congestion 時(shí)間: 2025-3-30 11:54 作者: giggle 時(shí)間: 2025-3-30 14:41 作者: Compass 時(shí)間: 2025-3-30 18:28 作者: 現(xiàn)實(shí) 時(shí)間: 2025-3-30 21:34 作者: Arroyo 時(shí)間: 2025-3-31 03:04
Gene Identification Using the Pufferfish, ,, by Sequence Scanning,our genome. Herein lies one of the major problems. A 3% return on investment, even when genes are identifiable, is rather poor, especially when sequencing is an expensive business. As if that is not enough, a large percentage of highly reiterated dispersed repeats serves to exacerbate the problem.作者: 使尷尬 時(shí)間: 2025-3-31 05:45 作者: pellagra 時(shí)間: 2025-3-31 10:50
Die Kollapstherapie der Lungentuberkulose,wing a normal phenotype, with a penetrance parameter that is specified for each genotype (..). Family studies using these techniques have led to the localization of many hundreds of single gene disorders (.) and an appreciable fraction of those localized have been positionally cloned.作者: Osteons 時(shí)間: 2025-3-31 15:46 作者: Intercept 時(shí)間: 2025-3-31 20:47 作者: 未成熟 時(shí)間: 2025-4-1 01:05 作者: 權(quán)宜之計(jì) 時(shí)間: 2025-4-1 04:09 作者: 易于 時(shí)間: 2025-4-1 08:02
Direct Selection of cDNAs by Genomic Clones,Once genomic DNA sequences become available, . gene identification will be the most widely used method. However, established techniques for gene identification will still be relevant, especially to identify specific genes, mapped to defined regions of the genome.