標題: Titlebook: Genomic Imprinting; Methods and Protocol Nora Engel Book 2012 Springer Science+Business Media, LLC 2012 Bioinformatics.Epigenetic profiles. [打印本頁] 作者: 租期 時間: 2025-3-21 18:09
書目名稱Genomic Imprinting影響因子(影響力)
書目名稱Genomic Imprinting影響因子(影響力)學科排名
書目名稱Genomic Imprinting網(wǎng)絡(luò)公開度
書目名稱Genomic Imprinting網(wǎng)絡(luò)公開度學科排名
書目名稱Genomic Imprinting被引頻次
書目名稱Genomic Imprinting被引頻次學科排名
書目名稱Genomic Imprinting年度引用
書目名稱Genomic Imprinting年度引用學科排名
書目名稱Genomic Imprinting讀者反饋
書目名稱Genomic Imprinting讀者反饋學科排名
作者: Hdl348 時間: 2025-3-21 21:37 作者: incarcerate 時間: 2025-3-22 01:45 作者: Paleontology 時間: 2025-3-22 04:33 作者: encyclopedia 時間: 2025-3-22 11:36 作者: 有權(quán) 時間: 2025-3-22 16:08 作者: 有權(quán) 時間: 2025-3-22 18:37
Data Mining as a Discovery Tool for Imprinted Geneseralized linear models (glm) to predicted imprinted status. This is meant to be an introduction to the method, so only the most straightforward examples will be covered. For instance, the examples given below refer to 11 classes of genomic regions (the entire gene body, introns, exons, 5′ UTR, 3′ UT作者: 消音器 時間: 2025-3-22 22:56 作者: Incommensurate 時間: 2025-3-23 01:22
Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cellse is that genomic DNA is randomly sheared by sonication and immunoprecipitated with an antibody that specifically recognizes 5-methylcytidine (5mC), which can be combined with PCR or high-throughput analysis (microarrays, deep sequencing). The MeDIP technique has been originally used to generate DNA作者: 有節(jié)制 時間: 2025-3-23 08:08 作者: arbiter 時間: 2025-3-23 09:44 作者: Adulate 時間: 2025-3-23 16:49 作者: 無畏 時間: 2025-3-23 18:53 作者: DALLY 時間: 2025-3-23 23:57
Generation of cDNA Libraries from RNP-Derived Regulatory Noncoding RNAs ncRNA species. Here, we describe the generation of a ncRNA-derived complementary DNA (cDNA) library by 3′-tailing of ncRNAs by CTP and poly(A) polymerase, followed by 5′-adapter ligation by T4 RNA ligase and reverse transcription of ncRNAs with an oligo-d(G) anchor primer. Preliminary selection of 作者: cocoon 時間: 2025-3-24 05:04 作者: 漂泊 時間: 2025-3-24 08:50
Data Mining as a Discovery Tool for Imprinted Geneson computational methods, including basic programming. However, this chapter is not meant to be an introduction to programming. Throughout the chapter, the reader will be provided with example code in the Perl programming language.作者: BOAST 時間: 2025-3-24 14:11
Book 2012ree decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In .Genomic Imprinting: Methods and Protocols., experts in the field provide a survey of the technologies that are being applied 作者: Seminar 時間: 2025-3-24 16:48 作者: 積習已深 時間: 2025-3-24 20:07
Sami Oikarinen M.D.,Maarit Oikarinens from mouse blastocyst stage embryos. Using this powerful in vitro system, scientists can now begin to tease apart the epigenetic processes that result in placental patterns of imprinted gene expression and begin to better understand the role these genes play in development and disease.作者: Initial 時間: 2025-3-24 23:35 作者: figurine 時間: 2025-3-25 05:28 作者: 預定 時間: 2025-3-25 09:02 作者: 北極人 時間: 2025-3-25 13:25
Diabetes bei Kindern und Jugendlichenmic imprinting is a special case of AI where bias is toward parental sex and can be identified by transcriptome sequencing of systems that represent reciprocally inherited loci. The focus of this protocol is on experimental design, analysis, and interpretation of genomic imprint discovery using whole transcriptome sequencing.作者: 免費 時間: 2025-3-25 18:52
Contraception for Women with Diabetes, methylation profiles on a genome scale in mammals and plants. Here we provide an optimized version of the MeDIP protocol suitable for low amounts of DNA, which can be used to study DNA methylation in cellular populations available in small quantities.作者: CARK 時間: 2025-3-25 23:48
Organisation der Diabetikerbetreuunge (3C) assay is a powerful technique to detect looping structures and assess the probability of interaction between distant genomic elements (1–3). Here we describe the 3C methodology, its power, and limitations, together with the controls and normalization steps required for an accurate analysis.作者: FACT 時間: 2025-3-26 01:39 作者: Muscularis 時間: 2025-3-26 06:38 作者: Capitulate 時間: 2025-3-26 09:48 作者: 友好關(guān)系 時間: 2025-3-26 15:35 作者: Outwit 時間: 2025-3-26 17:39
Quantitative Chromosome Conformation Capturee (3C) assay is a powerful technique to detect looping structures and assess the probability of interaction between distant genomic elements (1–3). Here we describe the 3C methodology, its power, and limitations, together with the controls and normalization steps required for an accurate analysis.作者: 含鐵 時間: 2025-3-26 21:32 作者: LOPE 時間: 2025-3-27 03:02
Epidemiology and Pathophysiology, based on in vivo recombination, therefore limiting the number of steps performed in ES cells and allowing to take advantage of the growing number of .P insertional mutations already available in transgenic mice.作者: COM 時間: 2025-3-27 06:03 作者: Texture 時間: 2025-3-27 09:32 作者: Eulogy 時間: 2025-3-27 15:53
Engineering of Large Deletions and Duplications In Vivo based on in vivo recombination, therefore limiting the number of steps performed in ES cells and allowing to take advantage of the growing number of .P insertional mutations already available in transgenic mice.作者: 無王時期, 時間: 2025-3-27 18:43
Chromatin Immunoprecipitation to Characterize the Epigenetic Profiles of Imprinted Domainssmall number of cells. We provide examples to show that this ChIP protocol can specifically distinguish between parental alleles in mouse embryo fibroblasts carrying maternal and paternal duplication of mouse distal Chr7 and also in normal mouse embryo fibroblasts carrying single nucleotide polymorphism at imprinted regions.作者: 細胞學 時間: 2025-3-27 22:00
Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytesrary. Following RNA isolation, DNA is precipitated, isolated, and bisulfite converted for DNA methylation studies. Our results demonstrate the feasibility of isolating RNA and DNA from a small number of cells with repeatability of results.作者: 整頓 時間: 2025-3-28 02:49
Generation of Trophoblast Stem Cellss from mouse blastocyst stage embryos. Using this powerful in vitro system, scientists can now begin to tease apart the epigenetic processes that result in placental patterns of imprinted gene expression and begin to better understand the role these genes play in development and disease.作者: Pulmonary-Veins 時間: 2025-3-28 09:04
Immunomagnetic Purification of Murine Primordial Germ Cellsody recognizing an epitope characteristic of pluripotent stem cells. After reaction with a paramagnetic bead-linked secondary antibody, the cell mixture is applied to a strong magnetic field. PGCs are recovered by release from the magnetic field. Purity is assessed by the alkaline phosphatase activity inherent to PGCs.作者: CAND 時間: 2025-3-28 11:36
Generation of cDNA Libraries from RNP-Derived Regulatory Noncoding RNAsrase, followed by 5′-adapter ligation by T4 RNA ligase and reverse transcription of ncRNAs with an oligo-d(G) anchor primer. Preliminary selection of ncRNAs from ribonucleoprotein particles (RNPs) enables a strong enrichment of the generated libraries with functional regulatory ncRNAs compared to classical approaches.作者: Suppository 時間: 2025-3-28 16:02
1064-3745 oductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?.Authorita978-1-4939-5966-2978-1-62703-011-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 缺陷 時間: 2025-3-28 18:54 作者: 基因組 時間: 2025-3-29 01:35 作者: 有毛就脫毛 時間: 2025-3-29 05:48
https://doi.org/10.1007/978-3-662-11466-7ive real-time PCR or high-throughput sequencing can be used, giving either measurement of methylation at a single locus or across the entire genome, respectively. While the data produced by single locus assays is relatively simple to analyze and interpret, global assays such as microarrays or high-t作者: BROOK 時間: 2025-3-29 10:53
Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Speciesy reprogrammed iPSCs efficiently. Common marmoset (.), a nonhuman primate, represents an alternative model to the usual laboratory animals. Finally, patient-specific human iPSCs give us an opportunity to examine the pathology and mechanisms of dysregulated genomic imprinting. The iPSC technology wil作者: Picks-Disease 時間: 2025-3-29 13:53
Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNAive real-time PCR or high-throughput sequencing can be used, giving either measurement of methylation at a single locus or across the entire genome, respectively. While the data produced by single locus assays is relatively simple to analyze and interpret, global assays such as microarrays or high-t作者: minion 時間: 2025-3-29 15:37
https://doi.org/10.1007/978-1-62703-011-3Bioinformatics; Epigenetic profiles; Gene regulation; Genomic evolution; Imprinted genes; Parent-of-origi作者: Excise 時間: 2025-3-29 23:36
Nora EngelSurveys cutting-edge technologies applied in the field.Features detailed and practical laboratory protocols.Includes expert tips on troubleshooting and avoiding pitfalls.Includes supplementary materia作者: IRS 時間: 2025-3-30 01:56
978-1-4939-5966-2Springer Science+Business Media, LLC 2012作者: VALID 時間: 2025-3-30 07:06
Genomic Imprinting978-1-62703-011-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 無情 時間: 2025-3-30 11:35
Allison J. Hahr M.D.,Mark E. Molitch M.D.mic sperm injection, and round spermatid injection. By generating uniparental embryos that have exclusively paternal or maternal genomes, it is possible to study the functions of the parental genomes separately. It is possible to compare functions in haploid and diploid states. In addition, nuclear 作者: Barrister 時間: 2025-3-30 15:21
Diabetes and Protein Glycosylationo generate pluripotent stem cells directly from somatic cells, and these cells were named induced pluripotent stem cells (iPSCs). This method consists of technically simple procedures: donor cell preparation, gene transduction, and isolation of embryonic stem cell-like colonies. The iPSC technology 作者: Pulmonary-Veins 時間: 2025-3-30 17:52
Sami Oikarinen M.D.,Maarit Oikarinenish and maintain lineage-specific, monoallelic patterns of gene expression. This chapter describes the isolation an culture of trophectoderm stem cells from mouse blastocyst stage embryos. Using this powerful in vitro system, scientists can now begin to tease apart the epigenetic processes that resu作者: Decrepit 時間: 2025-3-30 22:08
Diabetes and the Endocrine Pancreasor the immunopurification of PGCs from the mouse embryo. After dissection and disruption of the fetal gonad, PGCs are identified by a monoclonal antibody recognizing an epitope characteristic of pluripotent stem cells. After reaction with a paramagnetic bead-linked secondary antibody, the cell mixtu作者: brassy 時間: 2025-3-31 01:28 作者: VERT 時間: 2025-3-31 05:31
Diabetes bei Kindern und Jugendlichenant approach for mapping allelic imbalance (AI), where transcription is biased toward one allele in a diploid system. AI is identified by counting sequencing reads that map to genomic regions containing heterozygous SNPs, where the base identity of the SNP is used to distinguish allelic origin. Geno作者: CAGE 時間: 2025-3-31 11:33
Thomas Danne,Olga Kordonouri,Karin Langeeralized linear models (glm) to predicted imprinted status. This is meant to be an introduction to the method, so only the most straightforward examples will be covered. For instance, the examples given below refer to 11 classes of genomic regions (the entire gene body, introns, exons, 5′ UTR, 3′ UT
