標(biāo)題: Titlebook: Genome Instability; Methods and Protocol Marco Muzi-Falconi,Grant W Brown Book 2018 Springer Science+Business Media LLC 2018 eukaryotes.tel [打印本頁] 作者: 威風(fēng) 時(shí)間: 2025-3-21 16:33
書目名稱Genome Instability影響因子(影響力)
書目名稱Genome Instability影響因子(影響力)學(xué)科排名
書目名稱Genome Instability網(wǎng)絡(luò)公開度
書目名稱Genome Instability網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Genome Instability被引頻次
書目名稱Genome Instability被引頻次學(xué)科排名
書目名稱Genome Instability年度引用
書目名稱Genome Instability年度引用學(xué)科排名
書目名稱Genome Instability讀者反饋
書目名稱Genome Instability讀者反饋學(xué)科排名
作者: 輕彈 時(shí)間: 2025-3-21 22:29 作者: 宴會(huì) 時(shí)間: 2025-3-22 00:32
Study of UV-induced DNA Repair Factor Recruitment: Kinetics and Dynamics, only a very small portion of the nucleus of a cell by letting UV light pass only through the pores. Immunofluorescent analyses of modified DNA nucleotides, proteins, or fluorescently tagged versions of target factors can be used as markers to label and study UV-induced lesions and their repair.作者: 宏偉 時(shí)間: 2025-3-22 07:25 作者: SKIFF 時(shí)間: 2025-3-22 12:08 作者: 著名 時(shí)間: 2025-3-22 14:51
,Revisiting “Sordid Fantasies”,addition, I provide tools for analyzing data from fluctuation assays. This 96-well plate protocol has been optimized for use in yeast but should perform equally well for a range of microorganisms using standard microbiological methods.作者: 著名 時(shí)間: 2025-3-22 17:14 作者: sparse 時(shí)間: 2025-3-22 23:04 作者: Encoding 時(shí)間: 2025-3-23 02:27
,Measuring Mutation Rates Using the Luria-Delbrück Fluctuation Assay,addition, I provide tools for analyzing data from fluctuation assays. This 96-well plate protocol has been optimized for use in yeast but should perform equally well for a range of microorganisms using standard microbiological methods.作者: syring 時(shí)間: 2025-3-23 07:45
Molecular Genetic Characterization of Mutagenesis Using a Highly Sensitive Single-Stranded DNA Repoof long stretches of single-stranded DNA in budding yeast cells. This system is ~100- to ~1000-fold more susceptible to mutation than conventional double-stranded DNA reporters, and is well suited for generating large mutational datasets to investigate the properties of mutagens.作者: 大吃大喝 時(shí)間: 2025-3-23 12:10
Inserting Site-Specific DNA Lesions into Whole Genomes, method will be instrumental to study qualitatively and quantitatively the genetic consequences of site-specific lesions in vivo; moreover, it does also allow analyzing the molecular structure of stalled replication forks at well-defined locations.作者: 高原 時(shí)間: 2025-3-23 16:12
1064-3745 ation advice from the experts.Includes supplementary materia.This volume presents forty-two methods and protocols to analyze diverse aspects of genome instability. Chapters detail mutagenesis and repair, methods to quantify and analyze the properties of DNA double-strand breaks, profile replication,作者: 缺陷 時(shí)間: 2025-3-23 20:43
“Ecologic” Border and Deterritorialisationl using a counterselection step. The ALF assay relies on the ability to count spurious mating events that occur upon loss of the .α locus of haploid . strains. Here, we describe the deployment of the ALF assay for both rapid and simple qualitative, and more in-depth quantitative analysis allowing determination of absolute ALF frequencies.作者: 矛盾 時(shí)間: 2025-3-24 00:27
Curtis L. Ivery,Joshua A. Bassettd DNA nucleobases with single base precision. This protocol was used to map uracil incorporation and UV photodimers in DNA, and a modification of the protocol has been used to map sparse modification events in cells. The Excision-seq protocol is broadly applicable to a variety of base modifications for which an excision enzyme is available.作者: BILIO 時(shí)間: 2025-3-24 04:12
Hydrophobic Interaction in D2O Versus H2O only a very small portion of the nucleus of a cell by letting UV light pass only through the pores. Immunofluorescent analyses of modified DNA nucleotides, proteins, or fluorescently tagged versions of target factors can be used as markers to label and study UV-induced lesions and their repair.作者: Stress 時(shí)間: 2025-3-24 07:52 作者: maudtin 時(shí)間: 2025-3-24 11:41 作者: debase 時(shí)間: 2025-3-24 16:39
https://doi.org/10.1007/978-3-476-05863-8ter the separation by electrophoresis on alkaline denaturing agarose gel, these ssDNA fragments can be visualized by hybridization with an RNA probe that anneals with the 3′-undegraded DSB strand. This assay allows a direct and comprehensive visualization of DSB end processing.作者: monologue 時(shí)間: 2025-3-24 22:45 作者: TRACE 時(shí)間: 2025-3-25 01:23 作者: EVICT 時(shí)間: 2025-3-25 04:28 作者: 恩惠 時(shí)間: 2025-3-25 09:45 作者: Admonish 時(shí)間: 2025-3-25 12:23
Alkaline Denaturing Southern Blot Analysis to Monitor Double-Strand Break Processing,ter the separation by electrophoresis on alkaline denaturing agarose gel, these ssDNA fragments can be visualized by hybridization with an RNA probe that anneals with the 3′-undegraded DSB strand. This assay allows a direct and comprehensive visualization of DSB end processing.作者: 忍受 時(shí)間: 2025-3-25 19:12
Analysis of Replicative Polymerase Usage by Ribonucleotide Incorporation,olymerase. Changes to DNA polymerase usage can be examined at specific loci by Southern blot analysis while a global analysis of polymerase usage can be achieved by applying next-generation sequencing. This genome-wide data also provides a direct measure of replication origin efficiency and can be used to indirectly calculate replication timing.作者: 填滿 時(shí)間: 2025-3-25 20:14 作者: BIPED 時(shí)間: 2025-3-26 03:19 作者: 動(dòng)作謎 時(shí)間: 2025-3-26 04:51
“Ecologic” Border and Deterritorialisationlike faker (ALF) assay is one of several qualitative and quantitative marker loss assays that indirectly measure loss or conversion of genetic material using a counterselection step. The ALF assay relies on the ability to count spurious mating events that occur upon loss of the .α locus of haploid .作者: Brittle 時(shí)間: 2025-3-26 12:25
Robert J. Helfenbein,Edward O. Buendíaansmission and maintenance. The Chromosome Transmission Fidelity (CTF) colony color assay was developed to assess chromosome instability (CIN) in yeast, by monitoring the loss or gain during cell division of an artificial chromosome fragment carrying a visual marker. The CTF assay monitors changes i作者: Coronary 時(shí)間: 2025-3-26 15:28
,Revisiting “Sordid Fantasies”,sed to measure the mutation rate at a particular locus or loci at which mutations give rise to a selectable phenotype. Here, I outline the essential features of performing Luria-Delbrück fluctuation assays as well as common missteps and tips for improving the accuracy of mutation rate estimates. In 作者: 傀儡 時(shí)間: 2025-3-26 16:54
Detoxification of Chemical Warfare Agentson, which has yielded an amazing diversity of life on Earth. Mutations can also be the fundamental basis of serious human maladies, most notably cancers. In this chapter, I describe a highly sensitive reporter system for the molecular genetic analysis of mutagenesis, featuring controlled generation 作者: MILL 時(shí)間: 2025-3-26 21:59 作者: APNEA 時(shí)間: 2025-3-27 02:22 作者: Condescending 時(shí)間: 2025-3-27 07:00
Structural Characterization of Deuterides,us sources that can generate in the order of thousands of lesions a day in each of our cells (Lindahl, Nature 362(6422):709–715, 1993). DNA damages interfere with DNA metabolic processes such as transcription and replication and can be potent inhibitors of cell division and gene expression. To comba作者: Carminative 時(shí)間: 2025-3-27 10:27 作者: 大溝 時(shí)間: 2025-3-27 16:18
https://doi.org/10.1007/978-3-322-89670-4ation of a plasmid containing a site-specific lesion engineered in vitro into a precise location in the genome via the site-specific recombination reaction from phage lambda. The notion of DNA lesion is not restricted to chemically modified nucleotides but also refers to unusual DNA structures. This作者: Arthritis 時(shí)間: 2025-3-27 20:18
https://doi.org/10.1007/978-3-663-13575-3repair the break. This process is called DNA end resection and it is finely regulated to prevent genome rearrangements. Here, we describe a protocol to quantify DSB resection rate by qPCR, which could be applied to every organisms whenever the break site and its flanking region sequences are known.作者: ECG769 時(shí)間: 2025-3-28 00:49
https://doi.org/10.1007/978-3-476-05863-8gy-mediated repair pathways. Several methods have been developed to measure ssDNA accumulation at a DSB in the budding yeast .. Here, we describe one of these assays, which is based on the inability of restriction enzymes to cleave ssDNA. Digestion of genomic DNA prepared at different time points af作者: Hangar 時(shí)間: 2025-3-28 04:09
Die Bedeutung anderer Sprachen und Kulturen, of the break. The presence of resected DNA is an obligatory step for homologous recombination. Moreover, the amount of resected DNA modulates the prevalence of different recombination pathways. In different model organisms, there are several published ways to visualize and measure with more or less作者: 聯(lián)合 時(shí)間: 2025-3-28 09:23 作者: cleaver 時(shí)間: 2025-3-28 13:26
Deutsch als Zweitsprache im Schulsystem,, and recombination, or as a result of exogenous agents such as ionizing radiation, radiomimetic drugs, and genome editing nucleases. Unrepaired DSBs threaten genomic stability by leading to the formation of potentially oncogenic rearrangements such as translocations. In past few years, several meth作者: Mechanics 時(shí)間: 2025-3-28 18:35 作者: 人工制品 時(shí)間: 2025-3-28 19:46 作者: 個(gè)人長(zhǎng)篇演說 時(shí)間: 2025-3-29 00:24 作者: Harness 時(shí)間: 2025-3-29 04:34
https://doi.org/10.1007/978-3-642-05132-6herapy. The combination of psoralen crosslinking and electron microscopy has proven instrumental to reveal the fine architecture of in vivo DNA replication intermediates and to uncover their remodeling upon specific conditions of genotoxic stress. The replication structures are stabilized in vivo (b作者: Abduct 時(shí)間: 2025-3-29 08:11 作者: 投射 時(shí)間: 2025-3-29 11:57
DNA Replication Profiling Using Deep Sequencing,Profiling of DNA replication during progression through S phase allows a quantitative snap-shot of replication origin usage and DNA replication fork progression. We present a method for using deep sequencing data to profile DNA replication in ..作者: 和平主義 時(shí)間: 2025-3-29 17:19 作者: 收到 時(shí)間: 2025-3-29 21:27
Buchstabengruppe Sch / Im Klassenzimmerthods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topoisomerase 2 (Top2)-poisoning agent etoposide (ETO). These methods are powerful tools for the direct detection of the physiological and pathological “breakome” of the DNA in human cells.作者: BUOY 時(shí)間: 2025-3-30 03:51
A qPCR-Based Protocol to Quantify DSB Resection,repair the break. This process is called DNA end resection and it is finely regulated to prevent genome rearrangements. Here, we describe a protocol to quantify DSB resection rate by qPCR, which could be applied to every organisms whenever the break site and its flanking region sequences are known.作者: Cholagogue 時(shí)間: 2025-3-30 07:56
Mapping DNA Breaks by Next-Generation Sequencing,thods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topoisomerase 2 (Top2)-poisoning agent etoposide (ETO). These methods are powerful tools for the direct detection of the physiological and pathological “breakome” of the DNA in human cells.作者: POWER 時(shí)間: 2025-3-30 09:54
https://doi.org/10.1007/978-1-4939-7306-4eukaryotes; telomere maintenance; yeast; stem cell renewal; cancer biology作者: Prosaic 時(shí)間: 2025-3-30 15:08
978-1-4939-8447-3Springer Science+Business Media LLC 2018作者: 上流社會(huì) 時(shí)間: 2025-3-30 17:53
Genome Instability978-1-4939-7306-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: lavish 時(shí)間: 2025-3-30 22:45 作者: 商談 時(shí)間: 2025-3-31 03:13 作者: Boycott 時(shí)間: 2025-3-31 05:38 作者: 弄臟 時(shí)間: 2025-3-31 11:51 作者: AMITY 時(shí)間: 2025-3-31 17:18 作者: Myocyte 時(shí)間: 2025-3-31 19:19 作者: Triglyceride 時(shí)間: 2025-4-1 00:56 作者: milligram 時(shí)間: 2025-4-1 03:13
Study of UV-induced DNA Repair Factor Recruitment: Kinetics and Dynamics, the site of UV-induced DNA damage..Using Isopore filters with high density pores of a broad range of sizes, it is possible to UV irradiate and damage only a very small portion of the nucleus of a cell by letting UV light pass only through the pores. Immunofluorescent analyses of modified DNA nucleo作者: BLUSH 時(shí)間: 2025-4-1 06:55
Inserting Site-Specific DNA Lesions into Whole Genomes,ation of a plasmid containing a site-specific lesion engineered in vitro into a precise location in the genome via the site-specific recombination reaction from phage lambda. The notion of DNA lesion is not restricted to chemically modified nucleotides but also refers to unusual DNA structures. This作者: BRUNT 時(shí)間: 2025-4-1 11:10
A qPCR-Based Protocol to Quantify DSB Resection,repair the break. This process is called DNA end resection and it is finely regulated to prevent genome rearrangements. Here, we describe a protocol to quantify DSB resection rate by qPCR, which could be applied to every organisms whenever the break site and its flanking region sequences are known.作者: progestin 時(shí)間: 2025-4-1 15:49
