標(biāo)題: Titlebook: Genome Editing in Animals; Methods and Protocol Izuho Hatada Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under [打印本頁] 作者: OBESE 時間: 2025-3-21 19:11
書目名稱Genome Editing in Animals影響因子(影響力)
書目名稱Genome Editing in Animals影響因子(影響力)學(xué)科排名
書目名稱Genome Editing in Animals網(wǎng)絡(luò)公開度
書目名稱Genome Editing in Animals網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Genome Editing in Animals被引頻次
書目名稱Genome Editing in Animals被引頻次學(xué)科排名
書目名稱Genome Editing in Animals年度引用
書目名稱Genome Editing in Animals年度引用學(xué)科排名
書目名稱Genome Editing in Animals讀者反饋
書目名稱Genome Editing in Animals讀者反饋學(xué)科排名
作者: 食料 時間: 2025-3-21 22:50
Updated Overview of TALEN Construction Systems,mizable DNA-binding repeats and nuclease domain of FokI enzyme. Each DNA-binding repeat recognizes one base of double-strand DNA, and functional TALEN can be created by a simple modular assembly of these repeats. To easily and efficiently assemble the highly repetitive DNA-binding repeat arrays, var作者: 高興去去 時間: 2025-3-22 01:33
SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gin their causative genes. Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is a flexible and efficient genome engineering technology widely used for researches and therapeutic applications which offers immense opportunity to treat genetic dis作者: hazard 時間: 2025-3-22 05:37 作者: innovation 時間: 2025-3-22 09:19
Generation of Genome-Edited Mice by Cytoplasmic Injection of CRISPR-Cas9 RNA,ion of genome-edited animals. The Cas9/guide RNA (gRNA) component can be introduced into zygotes in several ways. Here, we provide an instructional guide for the generation of knockout mice using cytoplasmic injection of in vitro transcribed Cas9 RNA and gRNA.作者: LVAD360 時間: 2025-3-22 16:19 作者: LVAD360 時間: 2025-3-22 20:33
Generation of Knock-In Mouse by Genome Editing,ryonic stem cells, an exogenous DNA sequence can be inserted into the target locus in the zygote using genome-editing technology. In this chapter, I describe the generation of epitope-tagged mice using engineered endonuclease and single-strand oligodeoxynucleotide through the mouse zygote as an exam作者: Sciatica 時間: 2025-3-22 23:23 作者: 領(lǐng)巾 時間: 2025-3-23 04:57 作者: 最高峰 時間: 2025-3-23 06:36 作者: anachronistic 時間: 2025-3-23 10:19 作者: Blood-Clot 時間: 2025-3-23 17:33 作者: Apogee 時間: 2025-3-23 18:27
Efficient CRISPR/Cas9-Assisted Knockin of Large DNA Donors by Pronuclear Microinjection During S-Ph after the Cas9-induced double-strand DNA breaks; the activation of the DNA repair pathway is known to be correlated with the cell cycle. Recently, we have reported a new KI approach named SPRINT (.-phase .onuclear .jection for .argeting)-CRISPR, focusing on the correlation between the cell cycle an作者: apiary 時間: 2025-3-24 01:21
Genome Editing of Murine Liver Hepatocytes by AAV Vector-Mediated Expression of Cas9 In Vivo,s. The intravenous injection of an AAV vector harboring the gene of interest driven by the hepatocyte-specific promoter could efficiently express the target gene in liver hepatocytes. The delivery of genome editing tools including Cas9 and gRNA, by the AAV vector, can efficiently disrupt the target 作者: 拋射物 時間: 2025-3-24 06:02 作者: 墻壁 時間: 2025-3-24 09:20 作者: 完全 時間: 2025-3-24 11:32
A Simple and Efficient Method for Generating KO Rats Using In Vitro Fertilized Oocytes,ce. However, in rats, an efficient genome editing technique that uses in vitro fertilized oocytes has not been established. Recently, we reported the stable generation of offspring from five standard strains of rats by superovulation and in vitro fertilization (IVF). Furthermore, genome-edited rats 作者: promote 時間: 2025-3-24 16:53
Editing the Genome of the Golden Hamster (,),antageous in the study of reproductive and developmental biology: a highly stable 4-day estrous cycle, a high responsiveness to conventional superovulation methods, and a shortest gestation period (16?days) known among eutherian mammals. Besides these advantages, the technical ease of in vitro ferti作者: 手段 時間: 2025-3-24 20:14 作者: DEI 時間: 2025-3-25 02:08 作者: Nonflammable 時間: 2025-3-25 05:01 作者: Cpap155 時間: 2025-3-25 08:19 作者: set598 時間: 2025-3-25 15:18
Generation of Genome-Edited Mice by Cytoplasmic Injection of CRISPR-Cas9 RNA,ion of genome-edited animals. The Cas9/guide RNA (gRNA) component can be introduced into zygotes in several ways. Here, we provide an instructional guide for the generation of knockout mice using cytoplasmic injection of in vitro transcribed Cas9 RNA and gRNA.作者: 館長 時間: 2025-3-25 18:45
Nonviral Ex Vivo Genome Editing in Mouse Bona Fide Hematopoietic Stem Cells with CRISPR/Cas9,ventional gene therapy with lentivirus vectors that introduce genes into the genome randomly. Recent advancements in genome editing technology have substantially improved the knock-in efficiency, making it a reality. We present the details of a virus-free CRISPR/Cas9-based genome editing method for bona fide mouse hematopoietic stem cells.作者: 錢財 時間: 2025-3-25 21:27 作者: 江湖騙子 時間: 2025-3-26 02:11
Desk Reference for Neuroanatomymizable DNA-binding repeats and nuclease domain of FokI enzyme. Each DNA-binding repeat recognizes one base of double-strand DNA, and functional TALEN can be created by a simple modular assembly of these repeats. To easily and efficiently assemble the highly repetitive DNA-binding repeat arrays, var作者: 吃掉 時間: 2025-3-26 07:08 作者: 易彎曲 時間: 2025-3-26 10:26
https://doi.org/10.1007/978-3-662-21775-7ing from cross-species comparisons, naturally occurring variation in health and disease state to regulatory mechanisms..Although such perspectives are all informative to narrow down the list of genes or variants for perturbation experiments based on specific biological aims, utilizing multiple sourc作者: synchronous 時間: 2025-3-26 12:53 作者: 鞏固 時間: 2025-3-26 18:44
Gegenstand und Grundbegriffe der Statistik,eveloping gene knockout mice by inducing small indel mutations would be good enough, the successful ratio to create large side DNA knock-in (KI) by embryonic genome editing is still low. In contrast to the direct embryo KI method, gene targeting using embryonic stem cells (ESC) followed by chimeric 作者: 可卡 時間: 2025-3-26 23:44
https://doi.org/10.1007/978-3-8349-8779-2ryonic stem cells, an exogenous DNA sequence can be inserted into the target locus in the zygote using genome-editing technology. In this chapter, I describe the generation of epitope-tagged mice using engineered endonuclease and single-strand oligodeoxynucleotide through the mouse zygote as an exam作者: 取之不竭 時間: 2025-3-27 01:14
https://doi.org/10.1007/978-3-8349-4748-2nding disease pathophysiology. Recently, important advances in genome editing technologies have enabled us to efficiently create sophisticated animal models in short periods of time. Base editing is a modified CRISPR/Cas system that induces base substitution at targeted genomic regions. Here I descr作者: gentle 時間: 2025-3-27 06:41 作者: 改正 時間: 2025-3-27 11:44 作者: 聯(lián)合 時間: 2025-3-27 15:20
https://doi.org/10.1007/978-3-031-21274-1tegies for genotyping flox mice, typically based on Sanger sequencing following cloning of target sequences from dozens of pups, are time-consuming. Here, we describe a rapid screening method for flox mice, using in vitro Cre recombination that can be performed using simple enzymatic reactions and e作者: 愛國者 時間: 2025-3-27 21:46 作者: Memorial 時間: 2025-3-27 21:58
Professionelles Desktop Publishing, after the Cas9-induced double-strand DNA breaks; the activation of the DNA repair pathway is known to be correlated with the cell cycle. Recently, we have reported a new KI approach named SPRINT (.-phase .onuclear .jection for .argeting)-CRISPR, focusing on the correlation between the cell cycle an作者: Homocystinuria 時間: 2025-3-28 05:57
Desktop Publishing — Was bringt’s wirklich?s. The intravenous injection of an AAV vector harboring the gene of interest driven by the hepatocyte-specific promoter could efficiently express the target gene in liver hepatocytes. The delivery of genome editing tools including Cas9 and gRNA, by the AAV vector, can efficiently disrupt the target 作者: Encoding 時間: 2025-3-28 07:40 作者: 和藹 時間: 2025-3-28 12:21 作者: 琺瑯 時間: 2025-3-28 18:40
Ulrich Flasche,G. Dario Posada-Medranoce. However, in rats, an efficient genome editing technique that uses in vitro fertilized oocytes has not been established. Recently, we reported the stable generation of offspring from five standard strains of rats by superovulation and in vitro fertilization (IVF). Furthermore, genome-edited rats 作者: construct 時間: 2025-3-28 19:53 作者: byline 時間: 2025-3-29 02:21 作者: 無價值 時間: 2025-3-29 05:13
Genome Editing in Animals978-1-0716-3016-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 令人不快 時間: 2025-3-29 10:26 作者: abolish 時間: 2025-3-29 14:22
https://doi.org/10.1007/978-1-0716-3016-7Zinc Finger Nucleases; TALEN; CRISPR/Cas9; Embryonic Stem Cells; knockout mice作者: adjacent 時間: 2025-3-29 16:33 作者: 使乳化 時間: 2025-3-29 21:06 作者: Rotator-Cuff 時間: 2025-3-30 01:27 作者: nutrition 時間: 2025-3-30 05:45 作者: Lumbar-Spine 時間: 2025-3-30 10:30 作者: ATRIA 時間: 2025-3-30 15:40
Ulrich Flasche,G. Dario Posada-Medranoare collected from female rats under anesthesia, and COCs are induced into a medium containing concentration-adjusted sperm. Thereafter, oocytes with two pronucleus are selected as fertilized oocytes. Next, fertilized oocytes are transferred into a glass chamber containing CRISPR ribonucleoprotein (作者: 殺菌劑 時間: 2025-3-30 19:31 作者: corpuscle 時間: 2025-3-30 21:21 作者: 弄皺 時間: 2025-3-31 01:51
https://doi.org/10.1007/978-3-8349-8779-2escribe the generation of epitope-tagged mice using engineered endonuclease and single-strand oligodeoxynucleotide through the mouse zygote as an example of how to generate a knock-in mouse by genome editing.作者: SPECT 時間: 2025-3-31 05:57 作者: Anterior 時間: 2025-3-31 10:32
Marken, Variablen, Querverweise,regularly interspaced short palindromic repeats (CRISPR)-Cas9. Presently, genome edited strains have been produced by microinjection and a new electroporation method named technique for animal knockout system by . (.). This chapter presents the latest protocols for producing genome edited rats.作者: Carcinogen 時間: 2025-3-31 16:31 作者: 離開 時間: 2025-3-31 17:43 作者: Sciatica 時間: 2025-4-1 00:37
Desktop Publishing — Was bringt’s wirklich?nly by administering the AAV vector. The method could be suitable for developing genome editing treatments for inherited disorders and basic research exploring the physiological role of the target gene produced from liver hepatocytes.作者: Estrogen 時間: 2025-4-1 03:51 作者: 剛毅 時間: 2025-4-1 08:10
Generation of Floxed Mice by Sequential Electroporation,on at the one- and two-cell embryonic stages, respectively. This sequential electroporation method improves the floxing efficiency compared with the conventional simultaneous method, leading to a high yield of offspring with floxed alleles.作者: 充滿裝飾 時間: 2025-4-1 11:31
Genome Editing of Murine Liver Hepatocytes by AAV Vector-Mediated Expression of Cas9 In Vivo,nly by administering the AAV vector. The method could be suitable for developing genome editing treatments for inherited disorders and basic research exploring the physiological role of the target gene produced from liver hepatocytes.作者: Foolproof 時間: 2025-4-1 17:20
Care Industry Needs Skilled Migrant Labour,dure named single nucleotide polymorphism-distinguishable (SNPD)-CRISPR system which can suppress or enhance the expression of disease-causative gene with single nucleotide mutation distinguished from its wild-type. In this study, we used HRAS, one of most famous cancer-causative genes, as an example of a target gene.作者: intricacy 時間: 2025-4-1 21:02 作者: 憤怒歷史 時間: 2025-4-2 00:12
Grundlagen der digitalen Kartographie,this chapter, I describe the features of VCre/VloxP and SCre/SloxP, practical protocols and tips on how to use them in genomic engineering applications, potential problems in their use, and how problems can be identified and solved.
