作者: Omniscient 時間: 2025-3-21 21:21
CRISPR/Cas9 Approaches to Investigate the Noncoding Genome,omologous recombination in murine ES cells. Unfortunately, the study of the noncoding fraction of the genome did not benefit from the same valuable resources. Nevertheless, increasing evidence of the relevance of this fraction of the vertebrate genome has been accumulated in the past years. Comprehe作者: strain 時間: 2025-3-22 02:41 作者: CHURL 時間: 2025-3-22 07:47
CRISPR/Cas9 and the Paradigm Shift in Mouse Genome Manipulation Technologies,. CRISPR represents one of only a few radical and transformative shifts in transgenic technologies over the past 30 years. This chapter discusses the paradigm shift that CRISPR technology has brought about in the field of mouse genome editing.作者: 營養(yǎng) 時間: 2025-3-22 09:14 作者: 使痛苦 時間: 2025-3-22 14:17
Developments in the Generation of Reporter Stem Cells,s in drug screening, basic developmental research, and potential therapeutic implementations. An emerging need in stem cell biology is efficient and homogeneous differentiation of stem cells into mature, specialized, functional cells..Reporter stem cell lines are valuable models that enable noninvas作者: 使痛苦 時間: 2025-3-22 17:10
Current Status of Genome Editing in Cardiovascular Medicine,the Precision Medicine Initiative in January 2015, emerging technologies such as induced pluripotent stem cells (iPSCs) and genome editing are well positioned to provide powerful tools to correlate genotypes with phenotypes. These new technologies are helping to identify specific mutations associate作者: 縮減了 時間: 2025-3-22 22:37
Using CRISPR-Cas9 Genome Editing to Enhance Cell Based Therapies for the Treatment of Diabetes Mellng pancreatic β-cells for diabetes treatment. To generate these cells in vitro requires a complete understanding of the normal process of pancreas development: an objective greatly aided by CRISPR-Cas9 genome-editing technology. First identified as the adaptive immune system of bacteria, CRISPR-Cas9作者: tooth-decay 時間: 2025-3-23 05:03
Genome Editing in the Retina: A Case Study in CRISPR for a Patient-Specific Autosomal Dominant Retintions. The eye is an ideal target for gene therapy due to its accessibility, ease of noninvasive monitoring, significant compartmentalization, immunoprivileged status, optical transparency, and the presence of a contralateral control. One of the first gene therapy clinical trials was conducted in t作者: Euthyroid 時間: 2025-3-23 07:23
978-3-319-81687-6Springer International Publishing Switzerland 2016作者: 省略 時間: 2025-3-23 11:38 作者: 脾氣暴躁的人 時間: 2025-3-23 15:20
https://doi.org/10.1007/978-3-319-34148-4CRISPR; ESC; iPSC; nucleases; stem cells作者: 好忠告人 時間: 2025-3-23 21:37 作者: Endemic 時間: 2025-3-23 23:27 作者: 祝賀 時間: 2025-3-24 03:07 作者: 聯(lián)邦 時間: 2025-3-24 09:27 作者: MILL 時間: 2025-3-24 13:45
https://doi.org/10.1007/978-3-658-21388-6s a major technological advance in mammalian gene disruption. CRISPR/Cas enables genome editing by inducing targeted DNA double-strand breaks (DSBs) that are repaired by error-prone, nonhomologous end-joining (NHEJ), or homology-directed repair (HDR). This system has emerged as an effective tool for作者: 貪婪性 時間: 2025-3-24 18:40 作者: 制度 時間: 2025-3-24 19:04 作者: 確認 時間: 2025-3-25 00:11
Troubleshooting and Optimization,ng pancreatic β-cells for diabetes treatment. To generate these cells in vitro requires a complete understanding of the normal process of pancreas development: an objective greatly aided by CRISPR-Cas9 genome-editing technology. First identified as the adaptive immune system of bacteria, CRISPR-Cas9作者: uncertain 時間: 2025-3-25 06:12
Pat Langdon,Jonathan Lazar,Hua Dongntions. The eye is an ideal target for gene therapy due to its accessibility, ease of noninvasive monitoring, significant compartmentalization, immunoprivileged status, optical transparency, and the presence of a contralateral control. One of the first gene therapy clinical trials was conducted in t作者: ALIBI 時間: 2025-3-25 10:44
How Do Designers from Practice Design?. CRISPR represents one of only a few radical and transformative shifts in transgenic technologies over the past 30 years. This chapter discusses the paradigm shift that CRISPR technology has brought about in the field of mouse genome editing.作者: tattle 時間: 2025-3-25 12:49 作者: 樂章 時間: 2025-3-25 16:19 作者: 難管 時間: 2025-3-25 22:33 作者: 乞討 時間: 2025-3-26 00:14
his book is essential reading for all clinicians and researchers in stem cells, regenerative medicine, genomics, biochemicaland biomedical engineering- especially those interested in learning more about genome editing and applying it in a targeted, specific way..978-3-319-81687-6978-3-319-34148-4作者: 繁殖 時間: 2025-3-26 05:05
Diese Designer braucht das Land!, urgent need for accurate functional tests and genetic modelling of noncoding elements. In this chapter, we propose a number of strategies to test hypothesis regarding noncoding DNA elements, by taking advantage of the most recent genome editing techniques, namely, CRISPR/Cas9 approaches.作者: otic-capsule 時間: 2025-3-26 12:16
Understanding Design Cognition,used to edit the genome with base-pair precision, and methods for the detection and avoidance of off-target cleavage. Finally, we highlight sources of genetic and technical variation in iPSCs, and propose resolutions to the question of appropriate isogenic controls.作者: 陪審團每個人 時間: 2025-3-26 13:05 作者: micturition 時間: 2025-3-26 17:22
Middleware Memory Management in NoCzed because of the associated technical difficulty; however, new advances in genome-editing technologies has lowered these barriers for creating knock-in reporter lines. This chapter provides an overview of the methodology and potential applications of reporter stem cell lines.作者: enmesh 時間: 2025-3-26 22:36 作者: 哺乳動物 時間: 2025-3-27 01:59 作者: escalate 時間: 2025-3-27 08:01 作者: CT-angiography 時間: 2025-3-27 12:00
Developments in the Generation of Reporter Stem Cells,zed because of the associated technical difficulty; however, new advances in genome-editing technologies has lowered these barriers for creating knock-in reporter lines. This chapter provides an overview of the methodology and potential applications of reporter stem cell lines.作者: elucidate 時間: 2025-3-27 14:43 作者: Nomadic 時間: 2025-3-27 18:52
Book 2016se. The chapters describe latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular 作者: antipsychotic 時間: 2025-3-27 23:12
https://doi.org/10.1007/978-3-936560-43-5 to correct disease-causing mutations, modify crops and livestock, and perhaps modify our environment. This chapter discusses the advances in genome-editing technologies and their current and future applications.作者: hardheaded 時間: 2025-3-28 04:45
Troubleshooting and Optimization,J or via HDR. From the first demonstration that CRISPR-Cas can be programmed to cleave DNA in 2012, the field has advanced fast and now includes examples of targeting in many model organisms as well as gene knockout or reporter hPSC lines that will aid in the production of specific cell types, such as pancreatic β-cells.作者: CRUDE 時間: 2025-3-28 09:21
Pat Langdon,Jonathan Lazar,Hua Dongue as a viable treatment option for other inherited disorders across medical disciplines. This chapter highlights current ocular gene therapy approaches, clinical and preclinical experiments, and provides a case study of the bench-to-bedside personalized medicine approach taken for a novel and rare retinitis pigmentosa mutation.作者: Arboreal 時間: 2025-3-28 13:05
Genome Editing with Targetable Nucleases, to correct disease-causing mutations, modify crops and livestock, and perhaps modify our environment. This chapter discusses the advances in genome-editing technologies and their current and future applications.作者: 信任 時間: 2025-3-28 16:17
Using CRISPR-Cas9 Genome Editing to Enhance Cell Based Therapies for the Treatment of Diabetes MellJ or via HDR. From the first demonstration that CRISPR-Cas can be programmed to cleave DNA in 2012, the field has advanced fast and now includes examples of targeting in many model organisms as well as gene knockout or reporter hPSC lines that will aid in the production of specific cell types, such as pancreatic β-cells.作者: 作嘔 時間: 2025-3-28 19:32
Genome Editing in the Retina: A Case Study in CRISPR for a Patient-Specific Autosomal Dominant Retiue as a viable treatment option for other inherited disorders across medical disciplines. This chapter highlights current ocular gene therapy approaches, clinical and preclinical experiments, and provides a case study of the bench-to-bedside personalized medicine approach taken for a novel and rare retinitis pigmentosa mutation.作者: gout109 時間: 2025-3-29 02:18 作者: 有偏見 時間: 2025-3-29 04:43
