作者: BRIEF 時(shí)間: 2025-3-21 23:49 作者: ANA 時(shí)間: 2025-3-22 00:40
Calcium Imaging of Neural Activity in the Olfactory System of Drosophila,ation is subsequently encoded and processed by an overlapping, combinatorial activity of neurons forming complex neural circuits in the brain. In order to functionally dissect this neural circuitry, optical recording techniques allow visualizing spatial as well as temporal aspects of odor representa作者: 歌劇等 時(shí)間: 2025-3-22 04:45
Functional Imaging of Antennal Lobe Neurons in Drosophila with Synapto-pHluorin,ar memory traces are defined as any change in the activity of a neuron that is induced by learning, which subsequently alters the processing and response of the nervous system to the sensory information that is learned. Synapto-pHluorin (spH), a protein fusion of a pH-sensitive mutant of green fluor作者: Misgiving 時(shí)間: 2025-3-22 09:44 作者: 大氣層 時(shí)間: 2025-3-22 13:21
In Vivo Optical Recording of Brain Interneuron Activities from a Drosophila Male on a Treadmill,ous organisms, enabling the exploration of neural substrates for complex behaviors. As a showcase for such renovated neuroethology with GECIs, we describe a novel method to record optically interneuron activities from the brain of a tethered male fruit fly . when contact chemosensory stimulation is 作者: 大氣層 時(shí)間: 2025-3-22 17:58
Two-Photon Imaging of Population Activity with Genetically Encoded Calcium Indicators in Living Flies two-photon imaging of neural activity in an essentially intact fly. We present strategies to minimize motion of the brain, both in preparation technique and in apparatus design. We discuss key variables for reducing the problems of photobleaching and phototoxicity in order to collect high quality作者: 博識(shí) 時(shí)間: 2025-3-23 00:22
Engineering and Application of Genetically Encoded Calcium Indicators,n chimeric proteins consisting of a recognition element (e.g., a ligand-binding protein) and a reporter element (one or more fluorescent proteins). The analyte-induced conformational change in the recognition element leads to an observable change in fluorescence in the reporter element. Expression o作者: 萬(wàn)神殿 時(shí)間: 2025-3-23 05:19
Imaging cAMP Dynamics in the Drosophila Brain with the Genetically Encoded Sensor Epac1-Camps,unction. The development of genetically encoded sensors for cAMP has made it possible to measure cAMP dynamics within living cells with high temporal and spatial resolution. In this chapter, we review live-imaging methods for the measurement of relative cAMP levels within single neurons of the expla作者: Forsake 時(shí)間: 2025-3-23 07:59 作者: HPA533 時(shí)間: 2025-3-23 12:18
Imaging cAMP Dynamics in the Drosophila Brain with the Genetically Encoded Sensor Epac1-Camps,nted adult . brain. Specifically, we describe a detailed protocol for the monitoring of neuronal cAMP dynamics during bath application of neurotransmitters and neuromodulators using the genetically encoded cAMP sensor Epac1-camps and scanning laser confocal microscopy.作者: 親屬 時(shí)間: 2025-3-23 16:06
https://doi.org/10.1007/978-3-322-98850-8tracellular processes. In this chapter, we describe protocols allowing the expression of a GFP-aequorin fusion protein in acute brain slices and the bioluminescence recording of Ca. transients in single neurons, or multiple neurons simultaneously.作者: 后天習(xí)得 時(shí)間: 2025-3-23 21:52 作者: 相符 時(shí)間: 2025-3-24 02:03 作者: 共同給與 時(shí)間: 2025-3-24 06:26 作者: 陳腐思想 時(shí)間: 2025-3-24 06:37 作者: 臭名昭著 時(shí)間: 2025-3-24 13:13
https://doi.org/10.1007/978-3-642-91904-6measurements. Here, we describe methods used in the design, testing, optimization and application of genetically encoded biosensors, with a particular focus on the widely utilized calcium indicator GCaMP.作者: irradicable 時(shí)間: 2025-3-24 18:02
Engineering and Application of Genetically Encoded Calcium Indicators,measurements. Here, we describe methods used in the design, testing, optimization and application of genetically encoded biosensors, with a particular focus on the widely utilized calcium indicator GCaMP.作者: 極端的正確性 時(shí)間: 2025-3-24 22:46
Book 2012escent or bioluminescent proteins (genetically encoded) that are sensitive to calcium, have been developed to study neuronal activity, and especially groups of neurons, with the goal of mapping and deciphering the neural code underlying major neurophysiological functions.? .Genetically Encoded Funct作者: atrophy 時(shí)間: 2025-3-25 02:14 作者: 笨拙處理 時(shí)間: 2025-3-25 03:51 作者: SCORE 時(shí)間: 2025-3-25 10:55 作者: Brain-Waves 時(shí)間: 2025-3-25 12:29 作者: landmark 時(shí)間: 2025-3-25 19:14 作者: 同音 時(shí)間: 2025-3-25 22:41
,Betrachtungen über das Flugproblem,ar memory traces are defined as any change in the activity of a neuron that is induced by learning, which subsequently alters the processing and response of the nervous system to the sensory information that is learned. Synapto-pHluorin (spH), a protein fusion of a pH-sensitive mutant of green fluor作者: 燒瓶 時(shí)間: 2025-3-26 03:02
https://doi.org/10.1007/978-3-662-01386-1 the . brain. Two-photon imaging with GECIs allows calcium signals to be monitored in the entire adult fly central brain in vivo. It has recently become possible to perform two-photon imaging and electrophysiology in behaving flies during tethered flight (Maimon et al., Nat Neurosci 13:393–399, 2010作者: 凝視 時(shí)間: 2025-3-26 08:22 作者: Stagger 時(shí)間: 2025-3-26 09:43
,Bauleitung, bauausführende Firmen,es two-photon imaging of neural activity in an essentially intact fly. We present strategies to minimize motion of the brain, both in preparation technique and in apparatus design. We discuss key variables for reducing the problems of photobleaching and phototoxicity in order to collect high quality作者: 有法律效應(yīng) 時(shí)間: 2025-3-26 14:39 作者: VAN 時(shí)間: 2025-3-26 19:01 作者: Mystic 時(shí)間: 2025-3-26 21:42 作者: 閑逛 時(shí)間: 2025-3-27 04:32 作者: DENT 時(shí)間: 2025-3-27 07:52
Genetically Encoded Functional Indicators978-1-62703-014-4Series ISSN 0893-2336 Series E-ISSN 1940-6045 作者: dendrites 時(shí)間: 2025-3-27 11:42
Jean-René MartinPresents a comprehensive overview of the biophysical and biochemical properties of the titular probes.Features easy and accessible descriptions of the main imaging tools and methods now available.Incl作者: 脫離 時(shí)間: 2025-3-27 16:40 作者: Ingredient 時(shí)間: 2025-3-27 18:57
Springer-Verlag GmbH Berlin Heidelbergs. In this chapter we describe various available genetically encoded calcium sensors (GECIs) and the binary transcriptional systems available for . to express these GECIs in olfactory neurons. We will explain step-by-step methods for fly brain preparation, introduce different odor application device作者: JAUNT 時(shí)間: 2025-3-27 23:30 作者: bromide 時(shí)間: 2025-3-28 03:49 作者: 有組織 時(shí)間: 2025-3-28 07:17
Book 2012antees successful results..?.Helpful and easy to use, .Genetically Encoded Functional Indicators. aims to inspire students and researchers and to serve as a useful guide to those who wish to start using these different brain imaging techniques and require a bit of guidance in how best to choose a te作者: 全部 時(shí)間: 2025-3-28 12:12 作者: 騷動(dòng) 時(shí)間: 2025-3-28 18:35
In Vivo Functional Brain Imaging Using a Genetically Encoded Ca2+-Sensitive Bioluminescence Reportetructures of the brain still remain difficult to record. This means that the development of other alternative or complementary techniques is still worthwhile. Recently a novel bioluminescence approach has been developed, allowing to functionally map, in vivo, neuronal activity and circuitry. The aim作者: 不給啤 時(shí)間: 2025-3-28 20:22 作者: dandruff 時(shí)間: 2025-3-28 23:32
