標(biāo)題: Titlebook: Genetic Transformation Systems in Fungi, Volume 1; Marco A. van den Berg,Karunakaran Maruthachalam Book 2015 Springer International Publis [打印本頁] 作者: 柳條筐 時(shí)間: 2025-3-21 19:32
書目名稱Genetic Transformation Systems in Fungi, Volume 1影響因子(影響力)
書目名稱Genetic Transformation Systems in Fungi, Volume 1影響因子(影響力)學(xué)科排名
書目名稱Genetic Transformation Systems in Fungi, Volume 1網(wǎng)絡(luò)公開度
書目名稱Genetic Transformation Systems in Fungi, Volume 1網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Genetic Transformation Systems in Fungi, Volume 1被引頻次
書目名稱Genetic Transformation Systems in Fungi, Volume 1被引頻次學(xué)科排名
書目名稱Genetic Transformation Systems in Fungi, Volume 1年度引用
書目名稱Genetic Transformation Systems in Fungi, Volume 1年度引用學(xué)科排名
書目名稱Genetic Transformation Systems in Fungi, Volume 1讀者反饋
書目名稱Genetic Transformation Systems in Fungi, Volume 1讀者反饋學(xué)科排名
作者: 形狀 時(shí)間: 2025-3-21 21:50
Defining the Maqasid for Measurementof efficient transformation systems has been hampered by the extreme diversity of ascomycetes, basidiomycetes, and zygomycetes. Polyethylene glycol-assisted transformation of fungal protoplasts is the most widely used system but alternative procedures, e.g., electroporation and .-mediated transforma作者: perpetual 時(shí)間: 2025-3-22 01:31 作者: AUGUR 時(shí)間: 2025-3-22 07:34 作者: 證實(shí) 時(shí)間: 2025-3-22 12:48 作者: entrance 時(shí)間: 2025-3-22 13:59
Intestinal Fluid Handling: Secretion genome technologies, which require handling of very large sized DNA molecules, the spheroplast method has once again become important. We present here the spheroplast method that achieved in the highest transformation efficiency of ..作者: entrance 時(shí)間: 2025-3-22 17:25 作者: 積習(xí)難改 時(shí)間: 2025-3-22 22:40 作者: PLUMP 時(shí)間: 2025-3-23 05:20
Beyond Subordination: Four Arguments into electro-competent (E-comp) . cells (Becker and Guarente, Methods Enzymol 194:182–187, 1991). Transformation of spheroplasts is more difficult (Cregg et al., Mol Cell Biol 5:3376–3385, 1985), and current methods to make chemically competent . cells results in significantly fewer transformants (作者: AVID 時(shí)間: 2025-3-23 07:04 作者: deforestation 時(shí)間: 2025-3-23 13:37 作者: 耕種 時(shí)間: 2025-3-23 16:42
https://doi.org/10.1007/978-1-4471-0471-1ng the time-consuming repetitive purification of protoplasts used for the widely applied polyethylene glycol-mediated method. Biolistic transformation is also relatively quick compared to other available methods and provides a high percentage of stable transformants.作者: Fibrillation 時(shí)間: 2025-3-23 20:37
https://doi.org/10.1007/978-1-4302-0666-8lmost universally applicable technique once suitable selection markers have been developed. In recent years the major technical advances has been made within the initial steps of the process, more specifically the efficient construction of plasmids for performing targeted genome modifications. This 作者: palpitate 時(shí)間: 2025-3-24 00:59 作者: fatuity 時(shí)間: 2025-3-24 04:42 作者: 行乞 時(shí)間: 2025-3-24 10:19
https://doi.org/10.1007/978-3-662-38172-4Uracil auxotrophs derived from the Zygomycete . through spontaneous mutation were transformed by biolistic particle bombardment. Effective biolistic transformation of intact spores was achieved using the Bio-Rad PDS-1000/He system. Transformation frequencies of up to several colonies per μg of circular DNA were obtained under optimized conditions.作者: 注意到 時(shí)間: 2025-3-24 11:44 作者: 散布 時(shí)間: 2025-3-24 18:18
Transformation of Zygomycete , Using Biolistic Particle BombardmentUracil auxotrophs derived from the Zygomycete . through spontaneous mutation were transformed by biolistic particle bombardment. Effective biolistic transformation of intact spores was achieved using the Bio-Rad PDS-1000/He system. Transformation frequencies of up to several colonies per μg of circular DNA were obtained under optimized conditions.作者: 偉大 時(shí)間: 2025-3-24 19:51
978-3-319-37970-8Springer International Publishing Switzerland 2015作者: 偽書 時(shí)間: 2025-3-25 02:59 作者: 發(fā)怨言 時(shí)間: 2025-3-25 07:24
Intestinal Fluid Handling: Secretion genome technologies, which require handling of very large sized DNA molecules, the spheroplast method has once again become important. We present here the spheroplast method that achieved in the highest transformation efficiency of ..作者: Biguanides 時(shí)間: 2025-3-25 08:39 作者: Oligarchy 時(shí)間: 2025-3-25 11:44 作者: 反話 時(shí)間: 2025-3-25 19:32
https://doi.org/10.1007/978-1-4471-0471-1ng the time-consuming repetitive purification of protoplasts used for the widely applied polyethylene glycol-mediated method. Biolistic transformation is also relatively quick compared to other available methods and provides a high percentage of stable transformants.作者: Ledger 時(shí)間: 2025-3-25 19:59 作者: 征服 時(shí)間: 2025-3-26 02:28 作者: GROVE 時(shí)間: 2025-3-26 06:51
Biolistic Transformation of , for Homoplasmic Mitochondrial Genome Transformantss located on the genome and many important metabolism functions associated with mitochondria, such as energy metabolism, generation of reactive oxygen species. Here, we describe a method to transform the mitochondrial genome of . by biolistic transformation.作者: 收集 時(shí)間: 2025-3-26 11:23
Use of the Biolistic Particle Delivery System to Transform Fungal Genomesng the time-consuming repetitive purification of protoplasts used for the widely applied polyethylene glycol-mediated method. Biolistic transformation is also relatively quick compared to other available methods and provides a high percentage of stable transformants.作者: 葡萄糖 時(shí)間: 2025-3-26 15:00 作者: admission 時(shí)間: 2025-3-26 17:35
Mediated Transformationzing the AMT process with new fungal species. The chapter also includes a highly efficient vector construction system based on Uracil Specific Excisions Reagent (USER) cloning and specific PCR generated building blocks, which can be combined ad hoc to create complex plasmids in a single cloning step.作者: Analogy 時(shí)間: 2025-3-26 21:44
2198-7777 niques.Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will 作者: 激怒某人 時(shí)間: 2025-3-27 04:06 作者: 極小 時(shí)間: 2025-3-27 08:26 作者: 思想流動(dòng) 時(shí)間: 2025-3-27 12:19 作者: Leaven 時(shí)間: 2025-3-27 17:00 作者: GUILT 時(shí)間: 2025-3-27 19:25 作者: assail 時(shí)間: 2025-3-27 22:50 作者: 容易懂得 時(shí)間: 2025-3-28 02:09 作者: Oscillate 時(shí)間: 2025-3-28 10:01
Protoplast Transformation for Genome Manipulation in Fungig with established methods and for development of new protocols to be applied to species for which transformation has not yet been achieved. Thereafter, we briefly summarize tools for enhanced efficiency of transformation, which have been applied in combination with protoplast transformation.作者: GUILT 時(shí)間: 2025-3-28 13:00
Electroporation Mediated DNA Transformation of Filamentous Fungito be useful in the mutagenesis of specific DNA fragments in vivo. The filamentous fungi provide potential for innovation to identify new promoters and regulatory sequences and the application of genetic transformation promises a healthy future for fungal biotechnology.作者: Abutment 時(shí)間: 2025-3-28 15:59
Transformation of , Protoplastsr their purposes. Preparation and manipulation of protoplasts has become a key step to a more efficient transformation procedure for this fungus, which is used as model species in the Mucorales to develop fermentation processes for the production of β-carotene, other carotenoids, and essential oils like gamma-linolenic acid and biodiesel.作者: harpsichord 時(shí)間: 2025-3-28 18:50
Electroporation of ,regg et al., Mol Cell Biol 5:3376–3385, 1985), and current methods to make chemically competent . cells results in significantly fewer transformants (. protocols, 2nd edn, Totowa, 27–42). Following are protocols to prepare E-comp . cells, and to electroporate plasmid DNA into these cells to generate transformed cells.作者: 高度 時(shí)間: 2025-3-29 03:00 作者: 紅潤 時(shí)間: 2025-3-29 03:11
2198-7777 mammalian infection (.Penicillium marneffei. and .Candida.). .Genetic Transformation Systems in Fungi, Volume 1 .provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits. .978-3-319-37970-8978-3-319-10142-2Series ISSN 2198-7777 Series E-ISSN 2198-7785 作者: syncope 時(shí)間: 2025-3-29 10:57 作者: intangibility 時(shí)間: 2025-3-29 11:50
Accepting the truth of hard messagesith valuable phenotypes. These procedures should be essential tools to characterize the physiological roles of the increasing number of genes available as a result of the fungal genomic projects (.) (Grigoriev et al., Nucleic Acids Res 40:D26–32, 2012).作者: Benzodiazepines 時(shí)間: 2025-3-29 19:38
Fungal Transformation: From Protoplasts to Targeted Recombination Systemst fungi impaired in the NHEJ system allowed to select recombinants at homologous sites with high efficiency. These advances have contributed significantly to the progress in the molecular biology of fungi.作者: APRON 時(shí)間: 2025-3-29 21:18 作者: Expressly 時(shí)間: 2025-3-30 01:21 作者: 飛來飛去真休 時(shí)間: 2025-3-30 06:45 作者: 弄皺 時(shí)間: 2025-3-30 08:32
Transformation Methodsy metabolites with importance in pharmaceutical industry or in the biocontrol of significant phytopathogenic fungi (Cardoza et al., Curr Genet 34:50–59, 2005; Sivasithamparam and Ghisalberti, . and ., London, pp 139–191). Other . species produce enzymes with a remarkable industrial importance and ev作者: Pigeon 時(shí)間: 2025-3-30 12:22 作者: Fraudulent 時(shí)間: 2025-3-30 19:18
Transformation of ,: Spheroplast Method genome technologies, which require handling of very large sized DNA molecules, the spheroplast method has once again become important. We present here the spheroplast method that achieved in the highest transformation efficiency of ..作者: Ischemic-Stroke 時(shí)間: 2025-3-30 21:16 作者: 直覺沒有 時(shí)間: 2025-3-31 04:40
Chemical Transformation of ,ation-techniques, the lithium acetate-based protocol described below is easy to perform, requires basic lab equipment and reliably yields good number of the expected transformants. This method can also be adapted for high-throughput analysis purposes.作者: Cuisine 時(shí)間: 2025-3-31 08:48
Electroporation of , into electro-competent (E-comp) . cells (Becker and Guarente, Methods Enzymol 194:182–187, 1991). Transformation of spheroplasts is more difficult (Cregg et al., Mol Cell Biol 5:3376–3385, 1985), and current methods to make chemically competent . cells results in significantly fewer transformants (作者: 吸氣 時(shí)間: 2025-3-31 12:20
