標(biāo)題: Titlebook: Genetic Recombination; Reviews and Protocol Alan S. Waldman Book 2004 Humana Press 2004 [打印本頁] 作者: Agitated 時間: 2025-3-21 17:04
書目名稱Genetic Recombination影響因子(影響力)
作者: 出處 時間: 2025-3-22 00:12 作者: IRATE 時間: 2025-3-22 01:55 作者: 信條 時間: 2025-3-22 07:05
Detecting Carcinogens With the Yeast DEL Assayaddition to the arsenal of predictive tests for genotoxicity and carcinogenicity. This chapter provides an in-depth description of materials and methods for the yeast DEL assay from a user’s prospective and should allow the assay to be successfully deployed in any laboratory with basic microbiological capability and minimal user training.作者: 滔滔不絕地講 時間: 2025-3-22 12:36 作者: STYX 時間: 2025-3-22 14:50
Chromatin Immunoprecipitation to Investigate Protein-DNA Interactions During Genetic Recombination the protein. Polymerase chain reaction (PCR) is then used to determine the relative amounts of DNA associated with the protein of interest throughout the recombination event. This in vivo chemical crosslinking technique can be used to localize proteins to both double-strand breaks and recombination intermediates.作者: STYX 時間: 2025-3-22 20:31 作者: 陰謀 時間: 2025-3-22 21:40
Barriers to Change and Hope for the Future,cribe a protocol for increasing the frequency of TNE in the true yeast, ., through the use of nonspecific, carrier oligonucleotides. These molecules, when added to the reaction, increase the TNE frequency up to 25-fold in some cases, perhaps by providing a molecular trap to bind factors, which may inactivate the specific targeting oligos.作者: 得意人 時間: 2025-3-23 04:15
https://doi.org/10.1007/978-94-6351-029-5ealing of partially complementary oligonucleotides; and (2) a true recombination intermediate structure formed by RecA protein-mediated strand exchange. The use of these substrates in assays designed to detect Holliday junction branch migration and resolution activities is described.作者: pacifist 時間: 2025-3-23 06:25
DNA Fragment Transplacement in ,tions of transformation efficiency, length of homology, and alternative target site configuration were assessed. This analysis indicates that several genetic parameters are important for optimizing the efficiency of gene transplacement.作者: Archipelago 時間: 2025-3-23 12:15 作者: 古老 時間: 2025-3-23 17:13 作者: 忘恩負(fù)義的人 時間: 2025-3-23 19:41 作者: 細(xì)絲 時間: 2025-3-24 00:39 作者: 說明 時間: 2025-3-24 03:43 作者: Harrowing 時間: 2025-3-24 09:21 作者: motivate 時間: 2025-3-24 11:57
1064-3745 and undergo a transfer of information, producing new “recombinant” sequences that contain information from each of the original molecules. All organisms have the ability to carry out recombination, and this striking universality speaks to the essential role recombination plays in a variety of biolo作者: 深淵 時間: 2025-3-24 16:37
Mast Cell Disease (Urticaria Pigmentosa)nted in mouse hybridoma cells have several advantages that render them particularly amenable to gene targeting. Here, we present the basic methods currently in use in our laboratory that exploit this system to investigate mammalian gene targeting mechanisms.作者: Gobble 時間: 2025-3-24 22:11 作者: PRO 時間: 2025-3-25 02:13 作者: 決定性 時間: 2025-3-25 06:56 作者: 啜泣 時間: 2025-3-25 09:49 作者: 我的巨大 時間: 2025-3-25 12:25 作者: GLADE 時間: 2025-3-25 17:17
Wirtschaftspolitische Implikationenects of biological investigation, which has yielded insights into a number of fundamental, as well as novel, cellular processes in all organisms. In the last decade or so, genetic manipulation of trypanosomes has become possible through DNA transformation, allowing yet more detailed analysis of the 作者: 喃喃訴苦 時間: 2025-3-25 20:11 作者: macrophage 時間: 2025-3-26 02:18
,Covering’s Other Hidden Assault,ion. Reversion to histidine prototrophy results in deletion (DEL) of the disrupting sequence. The DEL assay has been used to study the effects of various DNA-damaging treatments on the frequency of deletion-recombination and has been shown to have a high level of sensitivity and specificity toward c作者: 談判 時間: 2025-3-26 07:05 作者: 造反,叛亂 時間: 2025-3-26 10:21
Mast Cell Disease (Urticaria Pigmentosa)eting, can be used as a tool to investigate mammalian homologous recombination mechanisms. The chromosomal immunoglobulin (Ig) genes as they are presented in mouse hybridoma cells have several advantages that render them particularly amenable to gene targeting. Here, we present the basic methods cur作者: 高調(diào) 時間: 2025-3-26 14:36 作者: osteopath 時間: 2025-3-26 18:36 作者: ADAGE 時間: 2025-3-26 21:59
Taktische Verhandlungsszenarienis possible to use site-specific reagents to produce a break or other recombinagenic damage at a unique site, which makes possible detailed analysis of the repair products. In addition, targeted mutagenesis and gene replacement are stimulated in the immediate vicinity of the break site. To utilize m作者: OVERT 時間: 2025-3-27 04:07 作者: 木訥 時間: 2025-3-27 07:59 作者: Cytology 時間: 2025-3-27 12:15
https://doi.org/10.1007/978-94-6351-029-5etween paired DNA molecules. Enzymes that specifically recognize and process these junctions are necessary for the formation of recombinant products. In the methods described here, we detail the in vitro construction of two types of Holliday junction: (1) a small synthetic junction formed by the ann作者: DENT 時間: 2025-3-27 17:35
Genetic Recombination978-1-59259-761-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: chiropractor 時間: 2025-3-27 21:06
Book 2004 genetic information between two nearly identical sequences is not expected to dramatically alter a genome. Recombination between dissimilar sequences, which does happen on occasion, may have such harmful consequences as chromosomal translocations, deletions, or inversions. For many organisms, it is作者: Supplement 時間: 2025-3-27 22:12
1064-3745 Recombination between dissimilar sequences, which does happen on occasion, may have such harmful consequences as chromosomal translocations, deletions, or inversions. For many organisms, it is978-1-61737-442-5978-1-59259-761-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 節(jié)約 時間: 2025-3-28 04:14
Anatomie en fysiologie van de edentate mond,tervals to depict the error in such experiments is also discussed. The application of these methods is illustrated using the intron-based inverted repeat recombination reporter system developed in our lab to study the regulation of homeologous recombination.作者: 難解 時間: 2025-3-28 08:31
Bob Verbraeck,Anneke van der Plaatstrates into cultured Chinese hamster ovary cells, and a method for determining rates of intra-chromosomal recombination between sequences contained within the integrated substrates. The general approach described here should be applicable to the study of a variety of aspects of recombination in virt作者: Instantaneous 時間: 2025-3-28 12:12 作者: intuition 時間: 2025-3-28 16:10
https://doi.org/10.1007/978-3-030-25789-7configuration of the repeated regions and whether selection for recombinants is imposed. Non-selective assays detect both HR and NHEJ events. This chapter focuses on the systems for delivering I-.I nuclease to mammalian cells and the strategies for detecting various outcomes of DSB repair.作者: Vertical 時間: 2025-3-28 19:43 作者: 哎呦 時間: 2025-3-29 00:27
https://doi.org/10.1057/9781137368928assay, mice are sacrificed at d 20, eyes are removed, and the wholemount RPE slides are prepared for eye-spot analysis. The frequency, size, and position relative to the optic nerve of the eye-spots are determined. This assay can be used to study the effect of environmental chemicals and physical ag作者: 燕麥 時間: 2025-3-29 06:14
https://doi.org/10.1007/978-1-4757-4917-5. In subsequent studies triplex formation was able to induce both intramolecular and intermolecular homologous recombination, revealing its potential application for gene therapy. Recent reports demonstrate the ability of these molecules to locate and modify their cognate sites in chromosomal DNA in作者: 龍卷風(fēng) 時間: 2025-3-29 07:41 作者: leniency 時間: 2025-3-29 12:25
Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cellstrates into cultured Chinese hamster ovary cells, and a method for determining rates of intra-chromosomal recombination between sequences contained within the integrated substrates. The general approach described here should be applicable to the study of a variety of aspects of recombination in virt作者: ALERT 時間: 2025-3-29 18:12
Intrachromosomal Homologous Recombination in , into the mutant background, how recombination frequencies are determined, and, if necessary, how cells carrying a restored .A gene can be isolated and propagated for molecular analysis of the particular recombination event.作者: 脫毛 時間: 2025-3-29 23:25 作者: acetylcholine 時間: 2025-3-30 00:21 作者: 絆住 時間: 2025-3-30 06:51 作者: 罐里有戒指 時間: 2025-3-30 12:02 作者: 揭穿真相 時間: 2025-3-30 15:36 作者: Indelible 時間: 2025-3-30 19:44 作者: Connotation 時間: 2025-3-30 21:22 作者: 愛社交 時間: 2025-3-31 04:21 作者: 可用 時間: 2025-3-31 08:39
Transformation of Monomorphic and Pleomorphic ,ects of biological investigation, which has yielded insights into a number of fundamental, as well as novel, cellular processes in all organisms. In the last decade or so, genetic manipulation of trypanosomes has become possible through DNA transformation, allowing yet more detailed analysis of the
