標題: Titlebook: Genetic Engineering in Eukaryotes; Paul F. Lurquin,Andris Kleinhofs Book 1983 Springer Science+Business Media New York 1983 ethics.food.ge [打印本頁] 作者: antibody 時間: 2025-3-21 17:33
書目名稱Genetic Engineering in Eukaryotes影響因子(影響力)
書目名稱Genetic Engineering in Eukaryotes影響因子(影響力)學科排名
書目名稱Genetic Engineering in Eukaryotes網絡公開度
書目名稱Genetic Engineering in Eukaryotes網絡公開度學科排名
書目名稱Genetic Engineering in Eukaryotes被引頻次
書目名稱Genetic Engineering in Eukaryotes被引頻次學科排名
書目名稱Genetic Engineering in Eukaryotes年度引用
書目名稱Genetic Engineering in Eukaryotes年度引用學科排名
書目名稱Genetic Engineering in Eukaryotes讀者反饋
書目名稱Genetic Engineering in Eukaryotes讀者反饋學科排名
作者: 外向者 時間: 2025-3-21 22:35 作者: 大量 時間: 2025-3-22 01:33
Teil II: Radikale Neuformulierung,n healthy individuals of the same species, but, after introduction into such individuals, they are replicated autonomously and cause the appearance of the characteristic disease syndrome (Diener, 1979a). Unlike viral nucleic acids, viroids are not encapsidated.作者: chance 時間: 2025-3-22 08:00 作者: Narcissist 時間: 2025-3-22 12:26 作者: rectum 時間: 2025-3-22 13:36
Viroids and Genetic Engineering,n healthy individuals of the same species, but, after introduction into such individuals, they are replicated autonomously and cause the appearance of the characteristic disease syndrome (Diener, 1979a). Unlike viral nucleic acids, viroids are not encapsidated.作者: rectum 時間: 2025-3-22 17:46 作者: Morphine 時間: 2025-3-23 01:04
https://doi.org/10.1007/978-3-322-98640-5The genetic manipulation of plant cells via DNA-mediated transformation has been a slow and frustrating enterprise.作者: 吊胃口 時間: 2025-3-23 03:12
Genetic Engineering of Plant Cells: A Rapid Overview,The genetic manipulation of plant cells via DNA-mediated transformation has been a slow and frustrating enterprise.作者: thyroid-hormone 時間: 2025-3-23 09:22
978-1-4684-4495-7Springer Science+Business Media New York 1983作者: 同音 時間: 2025-3-23 20:56
Das Haager übereinkommen von 2005stem which make this possible include the following: the availability of selectable markers; the ease of transformation of yeast with exogenous DNA; the integration of DNA into yeast chromosomes by homologous recombination; and the ease of genetic manipulation of yeast cells. As an illustration of s作者: Granular 時間: 2025-3-23 22:12 作者: 抱狗不敢前 時間: 2025-3-24 04:39 作者: invade 時間: 2025-3-24 08:38 作者: arboretum 時間: 2025-3-24 12:43
https://doi.org/10.1007/978-3-7091-6398-6romosomes. involves phagocytosis and there is a requirement for co-precipitation of the donor DNA. or chromosomes. with calcium phosphate to achieve optimal frequencies of gene transfer. In either method, transformants usually initially exhibit an unstable phenotype, and stabilization ultimately occ作者: Rotator-Cuff 時間: 2025-3-24 16:13 作者: 易改變 時間: 2025-3-24 21:54 作者: 吞下 時間: 2025-3-24 23:34
https://doi.org/10.1007/978-3-662-39798-5ent (Miller, 1981a). Among the features which make baculoviruses highly advantageous as recombinant DNA vector systems are (1) a covalently-closed, circular, nuclear-replicating DNA genome, (2) an extendable rod-shaped capsid, (3) a group of genes, involved in occlusion, that are nonessential for in作者: 潛移默化 時間: 2025-3-25 05:34 作者: 抒情短詩 時間: 2025-3-25 11:31
https://doi.org/10.1007/978-3-642-60225-2can be efficiently transferred into a wide variety of Gram-negative bacteria by a separate mobilizing plasmid. One of the vectors is a cosmid and can be used for cloning large DNA fragments. The vectors, which can be selected on the basis of kanamycin, spectinomycin, or chloramphenicol resistance, c作者: 動作謎 時間: 2025-3-25 14:21
https://doi.org/10.1007/978-3-0348-6112-0 which presently exist can be divided into two categories: 1) vectors which integrate within the host genome and 2) vectors which can be established as independent replicons in plant cells. The first category includes the T-DNA from . and the T-DNA from .. The second class of vectors comprises the c作者: ventilate 時間: 2025-3-25 18:36
Ich sehe was, was du nicht siehst ...,hese processes are associated with the incorporation of bacterial plasmid DNA (T-DNA), into the genome of the host plant. Much effort is underway to utilize these processes to incorporate useful genes into plants by using the bacterial plasmid as a transformation vector. In order for the processes t作者: 畢業(yè)典禮 時間: 2025-3-25 20:54
G. Rutishauser,M. J. Mihatsch,M. Ristng genetic engineering. A fragment of about 20 kb (called the T-region) of a 120 kb plasmid (called Ti plasmid for tumor-inducing plasmid) is transferred to the plant cell where it becomes covalently linked to the nuclear DNA (1–7). This integrated DNA has been named the T-DNA. Crown Gall cells have作者: dithiolethione 時間: 2025-3-26 02:56
Epidemiologie und Kausalfaktoren,ticipant, I have enjoyed observing the original and often virtuostic exploits of the competitors. My own interest, in the meantime, has been to study with a minimum of manipulation, a natural parasexual genetic system, which I believe constitutes a precedent for the use of genetic engineering to int作者: Evolve 時間: 2025-3-26 05:10
Teil II: Radikale Neuformulierung,n healthy individuals of the same species, but, after introduction into such individuals, they are replicated autonomously and cause the appearance of the characteristic disease syndrome (Diener, 1979a). Unlike viral nucleic acids, viroids are not encapsidated.作者: 串通 時間: 2025-3-26 11:44
Physiologische Bedeutung des Harzes,nilamellar vesicles prepared with the Ca. -EDTA chelation technique (LUV) have been used for delivering infective poliovirus particles(1) and RNA(2) into permissive (HeLa) and non permissive (CHO,L) cells. More recently Fraley et al.(3) have reported the encapsulation of simian virus 40(SV40) DNA in作者: 確定 時間: 2025-3-26 13:08 作者: 使糾纏 時間: 2025-3-26 18:37 作者: Anal-Canal 時間: 2025-3-26 21:18
Gene Organization and Regulation in ,. Evidence from the Cloning and Transformation of the , Gene Cg for .. complementation in . (Schweizer et al., 1981). In ., only the .. gene is expressed. The .. , .. and .. genes were detected on the basis of transformation back into Neurospora with these cosmid clones.作者: grenade 時間: 2025-3-27 01:34
,The Dominant Selective Marker APH 3′ and the Study of the Expression of the Cotransfected Gene,ransfection has been the discovery, by Perucho et al. (1980) that when two genes are transfected together into a cell, they are integrated via a “pekalasome” at the same locus on a chromosome. This allows, with the help of a selective medium, the survival of cells which often coexpress the marker ge作者: Nausea 時間: 2025-3-27 09:07
Construction and Transfer into Mammalian Cells of a Vector Containing Insect Histone Genes,ely that genomic function is also regulated by the structure and composition of the chromatin itself.. Thus, it seems reasonable to predict that a complete knowledge of the mechanisms regulating genomic activity in eukaryotic cells will only come when we understand both the structure and function of作者: STENT 時間: 2025-3-27 13:10 作者: Comprise 時間: 2025-3-27 17:21 作者: 下級 時間: 2025-3-27 19:21 作者: BROOK 時間: 2025-3-27 23:11 作者: heart-murmur 時間: 2025-3-28 03:51
Gene Organization and Regulation in ,. Evidence from the Cloning and Transformation of the , Gene Cne cluster. This cluster is involved in the catabolism of quinic acid and encodes three closely linked structural genes, ., ., and ., and a positively acting regulatory gene, ., Fig. 1 (Giles et al., 1978). The (. gene cluster is very tightly linked to . on the right arm of LG VII and so far has pro作者: drusen 時間: 2025-3-28 08:01 作者: 云狀 時間: 2025-3-28 13:00
Transformation and Storage of Competent Yeast Cells,ormed with plasmid DNA.. A new transformation procedure has been recently developed (K. Murata, in press) which reprsents a radical departure from previously published methods. This procedure employs lithium acetate instead of exogenous enzymes (i.e. glusulase, lyticase, zymolyase) to make yeast cel作者: 繁忙 時間: 2025-3-28 14:53 作者: SUGAR 時間: 2025-3-28 20:16 作者: 認識 時間: 2025-3-28 22:56 作者: 新娘 時間: 2025-3-29 03:48
Transfer of DNA into Higher Eukaryotic Cells Using Recombinant Vectors Based on Simian Virus 40 andods for introduction of DNA into mammalian cells ., more sensitive assays for vector function ., and new selectable markers for stable transformation of tissue culture cells .. In this article we present recent work from our laboratory relating to these areas.作者: 滔滔不絕的人 時間: 2025-3-29 10:35
Construction and Transfer into Mammalian Cells of a Vector Containing Insect Histone Genes,to many types of eukaryotic cells, a revolution has occurred in the level of our understanding of the way genes are regulated in living cells. Nonetheless, in spite of the impressive progress made concerning the role of DNA sequence in functions such as promotion, enhancement, initiation of transcri作者: obsolete 時間: 2025-3-29 13:08
An Insect Virus for Genetic Engineering: Developing Baculovirus Polyhedrin Substitution Vectors,ent (Miller, 1981a). Among the features which make baculoviruses highly advantageous as recombinant DNA vector systems are (1) a covalently-closed, circular, nuclear-replicating DNA genome, (2) an extendable rod-shaped capsid, (3) a group of genes, involved in occlusion, that are nonessential for in作者: phytochemicals 時間: 2025-3-29 18:27
Bacterial-Plant Gene Cloning Shuttle Vectors for Genetic Modification of Plants, This requirement can be achieved by the development of systems for the delivery and integration of foreign genes into the plant genome. Two potential gene delivery systems have been the focus of considerable attention: the Ti plasmid of . and the DNA plant virion cauliflower mosaic virus (CaMV).. A作者: Palpate 時間: 2025-3-29 23:04 作者: osteoclasts 時間: 2025-3-30 03:23
Construction of Recombinant Ti Plasmids Containing the ,M Gene from ,. ,, which presently exist can be divided into two categories: 1) vectors which integrate within the host genome and 2) vectors which can be established as independent replicons in plant cells. The first category includes the T-DNA from . and the T-DNA from .. The second class of vectors comprises the c作者: STYX 時間: 2025-3-30 07:09 作者: 防銹 時間: 2025-3-30 10:47
Regeneration of Plants from Crown Gall Cells A step in the T-DNA mediated genetic engineering of plng genetic engineering. A fragment of about 20 kb (called the T-region) of a 120 kb plasmid (called Ti plasmid for tumor-inducing plasmid) is transferred to the plant cell where it becomes covalently linked to the nuclear DNA (1–7). This integrated DNA has been named the T-DNA. Crown Gall cells have作者: WAX 時間: 2025-3-30 13:41
The Potential uses of , in the Genetic Engineering of Higher Plants: Nature Got There First,ticipant, I have enjoyed observing the original and often virtuostic exploits of the competitors. My own interest, in the meantime, has been to study with a minimum of manipulation, a natural parasexual genetic system, which I believe constitutes a precedent for the use of genetic engineering to int作者: 狂熱語言 時間: 2025-3-30 19:00
Viroids and Genetic Engineering,n healthy individuals of the same species, but, after introduction into such individuals, they are replicated autonomously and cause the appearance of the characteristic disease syndrome (Diener, 1979a). Unlike viral nucleic acids, viroids are not encapsidated.作者: 擴大 時間: 2025-3-30 20:58
Liposomes as a Tool for Introducing Biologically Active Viral Nucleic Acids into Plant Protoplasts,nilamellar vesicles prepared with the Ca. -EDTA chelation technique (LUV) have been used for delivering infective poliovirus particles(1) and RNA(2) into permissive (HeLa) and non permissive (CHO,L) cells. More recently Fraley et al.(3) have reported the encapsulation of simian virus 40(SV40) DNA in作者: 冷峻 時間: 2025-3-31 04:18 作者: 商店街 時間: 2025-3-31 09:02
Construction of Cloning Vectors from the ,W Plasmid pSa and their use in Analysis of Crown Gall Tumbe used for cloning large DNA fragments. The vectors, which can be selected on the basis of kanamycin, spectinomycin, or chloramphenicol resistance, contain cloning sites for seven different restriction endonucleases. DNA fragments from the .. . plasmid TiC58 have been cloned into these vectors, and used to complement avirulent TiC58::Tn5 mutants.作者: 打谷工具 時間: 2025-3-31 12:46
Construction of Recombinant Ti Plasmids Containing the ,M Gene from ,. ,,s independent replicons in plant cells. The first category includes the T-DNA from . and the T-DNA from .. The second class of vectors comprises the caulimovirus and germinivirus genomes, as well as cDNA copies of viroids. The pros and cons of each of those vectors have been recently reviewed by Howell..作者: transdermal 時間: 2025-3-31 17:25 作者: Feature 時間: 2025-3-31 20:32
The Potential uses of , in the Genetic Engineering of Higher Plants: Nature Got There First,with a minimum of manipulation, a natural parasexual genetic system, which I believe constitutes a precedent for the use of genetic engineering to introduce novel and potentially useful morphogenetic and physiological characters into plants.作者: 有組織 時間: 2025-3-31 21:46
Liposomes as a Tool for Introducing Biologically Active Viral Nucleic Acids into Plant Protoplasts,nto permissive (HeLa) and non permissive (CHO,L) cells. More recently Fraley et al.(3) have reported the encapsulation of simian virus 40(SV40) DNA in large unilamellar vesicles prepared with the reverse-phase evaporation technique (4) and its delivery in an infectious form to monkey cells in culture.作者: Criteria 時間: 2025-4-1 04:47 作者: 預知 時間: 2025-4-1 09:30 作者: BLUSH 時間: 2025-4-1 11:45
Use of Transformation and Meiotic Gene Conversion to Construct a Yeast Strain Containing a Deletionome of the features of the yeast system, we describe the construction of a yeast strain which contains a deletion of the 5′ end of the alcohol dehydrogenase I (ADHI) gene (.) of yeast. Deletions are, in general, rare events in yeast so the ability to introduce . created deletions into the yeast genome is quite useful.作者: emission 時間: 2025-4-1 18:13 作者: 震驚 時間: 2025-4-1 22:08
https://doi.org/10.1007/978-3-662-39798-5olyhedrin gene with passenger DNA was previously suggested as an approach to using baculoviruses as recombinant DNA vectors (Miller, 1981a). The initial experimental advances our laboratory has made in developing the baculovirus . nuclear polyhedrosis virus (AcNPV) as a vector in insect cells are described herein.作者: CAGE 時間: 2025-4-2 01:42
An Insect Virus for Genetic Engineering: Developing Baculovirus Polyhedrin Substitution Vectors,olyhedrin gene with passenger DNA was previously suggested as an approach to using baculoviruses as recombinant DNA vectors (Miller, 1981a). The initial experimental advances our laboratory has made in developing the baculovirus . nuclear polyhedrosis virus (AcNPV) as a vector in insect cells are described herein.作者: canonical 時間: 2025-4-2 05:10
Book 1983st 6, 1982. Although genetic engineering in eukaryotes is best developed in yeast and mammalian cells, the reader will find that some emphasis has been put on plant systems. Indeed, it was our position that the development of plant cell genetic transformation would benefit from the interactions betw作者: 骯臟 時間: 2025-4-2 09:19
Aktueller Stand der Forschung (Literatur),time required for the detection of transformants is dramatically reduced. We have adapted this procedure to allow the storage of competent yeast cells for up to 2 weeks at -70°C without significant loss of transformation efficiency.
