標(biāo)題: Titlebook: Genetic Engineering; Principles and Metho Jane K. Setlow Book 1987 Plenum Press, New York 1987 genetic engineering.Tree Biology [打印本頁] 作者: 掩飾 時(shí)間: 2025-3-21 17:23
書目名稱Genetic Engineering影響因子(影響力)
書目名稱Genetic Engineering影響因子(影響力)學(xué)科排名
書目名稱Genetic Engineering網(wǎng)絡(luò)公開度
書目名稱Genetic Engineering網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Genetic Engineering被引頻次
書目名稱Genetic Engineering被引頻次學(xué)科排名
書目名稱Genetic Engineering年度引用
書目名稱Genetic Engineering年度引用學(xué)科排名
書目名稱Genetic Engineering讀者反饋
書目名稱Genetic Engineering讀者反饋學(xué)科排名
作者: projectile 時(shí)間: 2025-3-21 23:37 作者: otic-capsule 時(shí)間: 2025-3-22 00:46
https://doi.org/10.1007/978-3-663-13653-8 such genes were originally discovered and studied in ., it is now clear that the response is an ancient and highly conserved one, found in all organisms (1–7). Heat shock proteins (hsps) are thought to protect cells from damage in certain stressful conditions, but the precise ways in which they do 作者: Accolade 時(shí)間: 2025-3-22 07:26
https://doi.org/10.1007/978-3-662-39833-3ne transfer system from . or modifications thereof (1,2). However, the recently developed system of protoplast electroporation is also being used as an effective method of gene transfer to both dicotyledonous and monocotyledonous species (3,4). There is a recent excellent review which thoroughly des作者: 供過于求 時(shí)間: 2025-3-22 09:14
,Eintheilung der Maa?e, Gewichte und Münzen, account for approximately half of the total seed protein. The storage proteins in maize seed are a group of prolamin proteins called zeins. Zeins are synthesized in the developing endosperm by membrane-bound polyribosomes and associate into insoluble aggregates called “protein bodies” within the lu作者: FIR 時(shí)間: 2025-3-22 13:01
Klimarekonstruktionen und Modelle,te information about the location of a protein binding site in the DNA sequence. In addition, the footprinting method provides a way of studying specific binding in the presence of a high nonspecific background, a common situation when working with rare proteins that have been only partially purifie作者: FIR 時(shí)間: 2025-3-22 18:27 作者: VEIL 時(shí)間: 2025-3-22 23:55
,Das physiologische Liquoreiwei?bild,ld be synthesized using either the SP6 or T7 promoter and an appropriate DNA duplex (1,2). However, several recent developments primarily centered on studies of intron splicing mechanisms (3–6) have led us to initiate a program in this area. We now feel that eventually new chemical methods for synth作者: 遺忘 時(shí)間: 2025-3-23 04:25
Das Verlegen und Verbinden von Kabeln,ermining the subunit structures of purified proteins (1). The two-dimensional gel system developed by O’Farrell, that combines isoelectric focusing in urea with SDS-PAGE, offers the additional advantage of greater resolution than SDS-PAGE alone (2,3). Even small amounts of proteins can be resolved a作者: miniature 時(shí)間: 2025-3-23 08:34 作者: 修飾語 時(shí)間: 2025-3-23 09:54
Die Theorie des Aktionspotentials,oorganisms and chemicals into the cells of ., . and . (1,2). Earlier reports had described the microdissection of animal cells (3,4), but these were the first experiments on the injection of material into living cells. The glass injection pipettes were pulled by hand in a microburner flame and posit作者: charisma 時(shí)間: 2025-3-23 17:51 作者: 歪曲道理 時(shí)間: 2025-3-23 18:33 作者: Palpable 時(shí)間: 2025-3-23 23:56
Das Verlegen und Verbinden von Kabeln,Plasmids are convenient vectors for the maintenance of cloned DNA. Inserts are stable, and large amounts of double-stranded (ds) DNA can be purified easily, manipulated .. with restriction and ligation enzymes, and the recombinant constructs reintroduced with high efficiency into .. . by transformation.作者: 方舟 時(shí)間: 2025-3-24 02:59 作者: sterilization 時(shí)間: 2025-3-24 07:18
978-1-4684-5379-9Plenum Press, New York 1987作者: FELON 時(shí)間: 2025-3-24 13:02
Genetic Engineering978-1-4684-5377-5Series ISSN 0196-3716 作者: Essential 時(shí)間: 2025-3-24 15:28 作者: 擔(dān)憂 時(shí)間: 2025-3-24 22:26 作者: 征稅 時(shí)間: 2025-3-24 23:27
Properties and Uses of Heat Shock Promoters, such genes were originally discovered and studied in ., it is now clear that the response is an ancient and highly conserved one, found in all organisms (1–7). Heat shock proteins (hsps) are thought to protect cells from damage in certain stressful conditions, but the precise ways in which they do 作者: 金絲雀 時(shí)間: 2025-3-25 03:46 作者: 嚙齒動物 時(shí)間: 2025-3-25 08:36 作者: Crepitus 時(shí)間: 2025-3-25 15:23 作者: esthetician 時(shí)間: 2025-3-25 17:18 作者: 緯線 時(shí)間: 2025-3-26 00:03
A New Method for Synthesizing RNA on Silica Supports,ld be synthesized using either the SP6 or T7 promoter and an appropriate DNA duplex (1,2). However, several recent developments primarily centered on studies of intron splicing mechanisms (3–6) have led us to initiate a program in this area. We now feel that eventually new chemical methods for synth作者: 滑動 時(shí)間: 2025-3-26 02:54
Activity Gels: Reformation of Functional Proteins in SDS-Polyacrylamide Gels,ermining the subunit structures of purified proteins (1). The two-dimensional gel system developed by O’Farrell, that combines isoelectric focusing in urea with SDS-PAGE, offers the additional advantage of greater resolution than SDS-PAGE alone (2,3). Even small amounts of proteins can be resolved a作者: 誘使 時(shí)間: 2025-3-26 07:42
High Level Production of Proteins in Mammalian Cells,ided a valuable aid to the understanding of the DNA sequence requirements for gene expression. In addition, this technology has provided a means to understand protein structure and function, by enabling the production of large amounts of proteins that previously could be isolated in only minute quan作者: 咒語 時(shí)間: 2025-3-26 09:18 作者: 不再流行 時(shí)間: 2025-3-26 15:27 作者: DIKE 時(shí)間: 2025-3-26 17:58
Alternative Splicing: Mechanistic and Biological Implications of Generating Multiple Proteins from These specific cell phenotypes result from the regulated expression of protein isoforms that are structurally distinct, developmentally regulated and cell specific. The molecular mechanisms responsible for the generation of this protein diversity fall into two main classes: those that select a part作者: 羊齒 時(shí)間: 2025-3-27 00:51
Chancen und Grenzen der Effizienzrevolution,e attraction of a developmental system that shares with the chordates a common if remote evolutionary ancestry. Definitive homologies in the morphogenesis of chordate and echinoderm embryos were noted by classical observers [reviewed in (3)], and in recent years specific molecular homologies have be作者: 杠桿 時(shí)間: 2025-3-27 03:54
,Eintheilung der Maa?e, Gewichte und Münzen, (2). When separated by 2-dimensional gel electrophoresis, the 22 and 19 kD components each resolve into a number of charged isomers, but the proteins of 27, 15, 14, and 10 kD migrate as single polypeptides. Based on immunocytochemical analysis, the 22 and 19 kD proteins are distributed throughout t作者: 情節(jié)劇 時(shí)間: 2025-3-27 05:18
,Karten — wo sind wir denn hier eigentlich?,n-2 (5) have been subjected to this approach. Other approaches have depended on enrichment of mRNA encoding the receptor; the low density lipoprotein receptor (6) and the T cell receptor (7) genes have been isolated by such means. We have employed an alternative approach which relies on DNA mediated作者: 談判 時(shí)間: 2025-3-27 12:27 作者: hidebound 時(shí)間: 2025-3-27 17:36
Das Verlegen und Verbinden von Kabeln,S-gels and transfer to nitrocellulose paper (4). This technique, though powerful, has the limitation of requiring antibodies, which usually necessitates prior purification of a specific protein of interest. An alternative approach is to detect isoproteins in a gel by their biochemical activities. Ou作者: 受人支配 時(shí)間: 2025-3-27 19:16 作者: excursion 時(shí)間: 2025-3-27 23:23
https://doi.org/10.1007/978-3-663-06918-8as depended upon the identification and isolation of a virus strain which is avirulent, or attenuated, relative to the indigenous strain which causes the disease problem in the field. Many of the mild virus strains used are generated by nitrous acid mutation of the virulent strain. Alternately, a va作者: extinct 時(shí)間: 2025-3-28 02:20
Gene Transfer in the Sea Urchin,e attraction of a developmental system that shares with the chordates a common if remote evolutionary ancestry. Definitive homologies in the morphogenesis of chordate and echinoderm embryos were noted by classical observers [reviewed in (3)], and in recent years specific molecular homologies have be作者: poliosis 時(shí)間: 2025-3-28 07:51 作者: Conduit 時(shí)間: 2025-3-28 14:14 作者: STANT 時(shí)間: 2025-3-28 16:07
A New Method for Synthesizing RNA on Silica Supports,serted at certain key sites so that their biochemical activity can be studied. We would envisage such a synthesis to involve first preparing the analogue portion of the RNA chemically and the remainder from DNA and either the T7 or SP6 promoter. These segments would then be joined with T4-RNA ligase作者: 陰謀 時(shí)間: 2025-3-28 20:39
Activity Gels: Reformation of Functional Proteins in SDS-Polyacrylamide Gels,S-gels and transfer to nitrocellulose paper (4). This technique, though powerful, has the limitation of requiring antibodies, which usually necessitates prior purification of a specific protein of interest. An alternative approach is to detect isoproteins in a gel by their biochemical activities. Ou作者: 描繪 時(shí)間: 2025-3-28 23:28 作者: Statins 時(shí)間: 2025-3-29 03:18
Genetic Transformation to Confer Resistance to Plant Virus Disease,as depended upon the identification and isolation of a virus strain which is avirulent, or attenuated, relative to the indigenous strain which causes the disease problem in the field. Many of the mild virus strains used are generated by nitrous acid mutation of the virulent strain. Alternately, a va作者: 考博 時(shí)間: 2025-3-29 09:58
Klimarekonstruktionen und Modelle,d. So effective is the footprinting method in discriminating between specific and nonspecific binding that it is often possible to visualize specific protein-DNA interactions with entirely unfractionated protein extracts from mammalian nuclei.作者: SEVER 時(shí)間: 2025-3-29 12:40 作者: Conserve 時(shí)間: 2025-3-29 19:32
DNase I Footprinting as an Assay for Mammalian Gene Regulatory Proteins,d. So effective is the footprinting method in discriminating between specific and nonspecific binding that it is often possible to visualize specific protein-DNA interactions with entirely unfractionated protein extracts from mammalian nuclei.作者: eustachian-tube 時(shí)間: 2025-3-29 22:36
High Level Production of Proteins in Mammalian Cells,tities, and by allowing the generation of proteins with specific, designed alterations. Although a variety of eukaryotic and prokaryotic host systems is available for the expression of a particular gene, the focus of this chapter is on the various approaches to the high level expression of heterologous genes in mammalian cells.作者: cochlea 時(shí)間: 2025-3-30 00:40
https://doi.org/10.1007/978-3-663-13653-8humans, ., nematodes, maize, soybean, yeast, and .. . (1–7, see Figure 1). All species examined have a protein of about 70 kD (hsp70), one about 80 to 90 kD (hsp83 in .), and usually several related small hsps (hsp22, hsp23, hsp26 and hsp27 in .).作者: 安裝 時(shí)間: 2025-3-30 07:55 作者: 吃掉 時(shí)間: 2025-3-30 10:35
Properties and Uses of Heat Shock Promoters,humans, ., nematodes, maize, soybean, yeast, and .. . (1–7, see Figure 1). All species examined have a protein of about 70 kD (hsp70), one about 80 to 90 kD (hsp83 in .), and usually several related small hsps (hsp22, hsp23, hsp26 and hsp27 in .).
