標(biāo)題: Titlebook: Generation of cDNA Libraries; Methods and Protocol Shao-Yao Ying Book 2003 Humana Press 2003 [打印本頁] 作者: Encounter 時間: 2025-3-21 16:29
書目名稱Generation of cDNA Libraries影響因子(影響力)
書目名稱Generation of cDNA Libraries影響因子(影響力)學(xué)科排名
書目名稱Generation of cDNA Libraries網(wǎng)絡(luò)公開度
書目名稱Generation of cDNA Libraries網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Generation of cDNA Libraries被引頻次
書目名稱Generation of cDNA Libraries被引頻次學(xué)科排名
書目名稱Generation of cDNA Libraries年度引用
書目名稱Generation of cDNA Libraries年度引用學(xué)科排名
書目名稱Generation of cDNA Libraries讀者反饋
書目名稱Generation of cDNA Libraries讀者反饋學(xué)科排名
作者: 聾子 時間: 2025-3-21 23:36
https://doi.org/10.1057/9780230316973protein. The basis of the widely used novel strategies for the generation of cDNA libraries are base pair complementarities, reverse transcription, and polymerase chain reactions. This chapter presents some general information on the principles of, biology behind, basic protocols of, and reagents us作者: Mobile 時間: 2025-3-22 00:29 作者: 分開 時間: 2025-3-22 06:42
Definitions and Conversions of Unitsmprehensive analysis is most frequently carried out on a single-clone-for-single-gene basis, whereas gene discovery is done through the isolation of multiple cDNA clones for a single gene. Thus, current requirements for high-quality cDNA libraries suitable for comprehensive cDNA analysis are (1) hig作者: byline 時間: 2025-3-22 10:06 作者: 小臼 時間: 2025-3-22 13:32
https://doi.org/10.1007/978-3-319-93236-1hods. A minimum of several thousand cells is needed for an acceptable quality of RNA extraction. Because of tissue heterogeneity, these methods usually provided neither reliable nor reproducible results. Unfortunately, it is impossible to collect adequate amounts of pure or homogeneous samples for t作者: 小臼 時間: 2025-3-22 17:06 作者: cataract 時間: 2025-3-22 23:20
Convex Bodies and Hypersurfaces structure at the 5′ end of eukaryotic mRNA to design novel methods for PCR-based cDNA library construction (.–.). Moreover, the strategy has been applied to identifying gene expression and isolating full-length cDNA (.–.). In our laboratory, the conventional and PCR-based strategies have been utili作者: insolence 時間: 2025-3-23 01:46 作者: vector 時間: 2025-3-23 07:57 作者: outrage 時間: 2025-3-23 11:29 作者: Amendment 時間: 2025-3-23 15:00 作者: 結(jié)束 時間: 2025-3-23 20:56
Construction of Size-Fractionated cDNA Library Assisted by an In Vitro Recombination Reaction,mprehensive analysis is most frequently carried out on a single-clone-for-single-gene basis, whereas gene discovery is done through the isolation of multiple cDNA clones for a single gene. Thus, current requirements for high-quality cDNA libraries suitable for comprehensive cDNA analysis are (1) hig作者: 散步 時間: 2025-3-24 00:17
Amplification of Representative cDNA Pools from Microscopic Amounts of Animal Tissue,demonstrated in our own experiments on differential display (.) and elsewhere in application of amplified cDNA as a probe for gene profiling by array technology (.–.). According to our experience in gene hunting in various biological models, amplified cDNA can substitute for normal, nonamplified cDN作者: COMA 時間: 2025-3-24 05:18
,mRNA/cDNA Library Construction Using RNA—Polymerase Cycling Reaction,hods. A minimum of several thousand cells is needed for an acceptable quality of RNA extraction. Because of tissue heterogeneity, these methods usually provided neither reliable nor reproducible results. Unfortunately, it is impossible to collect adequate amounts of pure or homogeneous samples for t作者: CANDY 時間: 2025-3-24 07:23 作者: 憤怒歷史 時間: 2025-3-24 11:22 作者: GRATE 時間: 2025-3-24 17:57
,Screening Poly [dA/dT(?)] cDNA for Gene Identification,ion/subtraction, poly(dA)–poly(dT) hybrids will form randomly between unrelated cDNA templates. The removal of these hybrids causes the loss of cDNA templates. This phenomenon affects especially the low-copy cDNA templates, which represent most of the genes. To overcome this problem, we developed th作者: 多山 時間: 2025-3-24 22:37 作者: Critical 時間: 2025-3-24 23:28 作者: 拋射物 時間: 2025-3-25 04:34 作者: 愛了嗎 時間: 2025-3-25 07:34 作者: 落葉劑 時間: 2025-3-25 15:21 作者: harmony 時間: 2025-3-25 19:37
https://doi.org/10.1057/9780230378827ailable. In essence, an adaptor with a defined sequence is attached to one end of the cDNA; then, the region between the adaptor and the known sequences is amplified by polymerase chain reaction (PCR). Since the initial publication in 1988 (.), RACE has greatly facilitated the cloning of new genes. 作者: Contend 時間: 2025-3-25 21:47 作者: ventilate 時間: 2025-3-26 01:12
Conversion and Environmental Conflictells. Some of them are expressed in most of the cells, but others are cell-or tissue-specific. It has been estimated that about 10,000 genes are expressed in a cell, but the abundance of their expression varies from 1 copy to 200,000 copies per cell (.). On average, the 10 most prevalent genes encod作者: bibliophile 時間: 2025-3-26 04:38 作者: 主講人 時間: 2025-3-26 10:08
Bernhard Brouqueyre,Jean Michel Tauziaolation, characterization, and analysis of both eukaryotic and prokaryotic genes. However, the conventional methods of cDNA cloning require much effort to generate a cDNA library and then screen for a large number of recombinant phages or plasmid clones. There are three major limitations in these me作者: Water-Brash 時間: 2025-3-26 16:23 作者: 根除 時間: 2025-3-26 20:24
https://doi.org/10.1057/9780230617858uman genomic sequence data. A number of attempts, which can be comprehensively termed “functional genomics,” are being carried out to decipher which parts of the human genome are transcribed, how the transcripts are spliced and translated, and what functions the eventual protein products conduct. Fo作者: sigmoid-colon 時間: 2025-3-27 00:08
https://doi.org/10.1007/978-0-85729-856-0polymerase chain reaction (PCR)-based methods. By incorporating a RNA promoter element during reverse transcription of messenger RNA (mRNA), a cDNA library can be preserved and amplified in the form of antisense RNA (aRNA) construct. In brief, the aRNA amplification procedure (. .) is based on (1) r作者: Accolade 時間: 2025-3-27 01:30
Transforming the Russian Economyte the fact that obtaining amounts of material suitable for direct processing by standard methods is often time-consuming and expensive and may be even impossible. Perhaps the most significant obstacle to the full appreciation of the technique is the widespread belief that polymerase chain reaction 作者: deface 時間: 2025-3-27 06:21 作者: 搏斗 時間: 2025-3-27 09:43
https://doi.org/10.1007/978-3-319-93236-1f the cells under certain special conditions, such as pathogenesis (.,.), cancer staging (.), drug treatment, and developmental processes (.). Traditionally, gene transcripts were extracted from lysed cells with pheno-chloroform followed by precipitation, and messenger RNAs (mRNA) were further purif作者: Contort 時間: 2025-3-27 16:48 作者: CHART 時間: 2025-3-27 19:40 作者: 讓你明白 時間: 2025-3-28 01:09
Settling in with the Ojibwe at Bad River,chain reaction (RT-PCR) (.,.). There are a number of variables that must be controlled for if accurate and reproducible results are to be obtained (.). A significant limitation to the accurate quantitation of many mRNA species is genomic DNA contamination during the RNA purification step (.). This c作者: mosque 時間: 2025-3-28 05:08
Islamophobia and the Talking Heads,ingle cells (.). By incorporating a RNA promoter element during the synthesis of double-stranded complementary DNA (cDNA) templates, a poly(A.) RNA library can be generated and reamplified from the templates in the same conformation and composition as its mRNA origins (.). Using microarray analysis,作者: 彩色的蠟筆 時間: 2025-3-28 07:46 作者: prolate 時間: 2025-3-28 10:44 作者: mortuary 時間: 2025-3-28 18:02 作者: Scleroderma 時間: 2025-3-28 22:45
Conversations with a Mathematicianlogy, is a very useful method for studying gene expression particularly when handling limited amounts of starting materials (. .). Using this method, analysis of gene expression can be carried out at a single-cell level (.–.). This is not possible using conventional techniques of mRNA isolation and cDNA cloning.作者: 迅速成長 時間: 2025-3-28 23:52 作者: instill 時間: 2025-3-29 03:50 作者: Heterodoxy 時間: 2025-3-29 10:56 作者: larder 時間: 2025-3-29 15:09 作者: quiet-sleep 時間: 2025-3-29 19:08
1064-3745 n extensively utilized to identify numerous gene probes in vitro and in vivo. However, attempts to generate complete and full-length complementary cDNA libraries were, for the most part, fruitless and remained elusive until the last decade, when simple and rapid methods were developed. With current 作者: Pessary 時間: 2025-3-29 21:49
https://doi.org/10.1057/9780230378827nd and an adaptor at the 5′ end are added to the first strand of cDNA during reverse transcription; amplification of virtually any transcript to either end can then make use of this same pool of cDNAs. In addition to being simple, the efficiency of 5′-RACE is dramatically increased because the adaptor is added only to full-length cDNAs.作者: RAG 時間: 2025-3-30 02:00
https://doi.org/10.1007/978-3-658-38509-5es of mRNAs from a limited amount of promoter-linked cDNAs (.,.) (. .). However, the aRNAs prepared from a single live neuron has been reported to cover 50–75% of the total mRNA population (.,.), indicating that rare mRNAs were lost during the amplification procedure.作者: Femine 時間: 2025-3-30 04:53 作者: 急性 時間: 2025-3-30 09:05
Single-Cell cDNA Library Construction Using Cycling aRNA Amplification,es of mRNAs from a limited amount of promoter-linked cDNAs (.,.) (. .). However, the aRNAs prepared from a single live neuron has been reported to cover 50–75% of the total mRNA population (.,.), indicating that rare mRNAs were lost during the amplification procedure.作者: 多產(chǎn)子 時間: 2025-3-30 12:43 作者: 燈泡 時間: 2025-3-30 17:27 作者: lesion 時間: 2025-3-30 21:34
https://doi.org/10.1057/9780230617858 unique resource because a variety of information about the gene functions is contained in a full-length cDNA sequence. The intensive analysis of a full-length cDNA would enable us to identify the following:作者: intolerance 時間: 2025-3-31 01:38
https://doi.org/10.1007/978-0-85729-856-0he promoter element of the cDNA to synthesize the aRNA sequence up to 2000-fold increase, (4) reverse transcription of the aRNA, (5) denaturation and then double-stranding the resulting cDNA with promoter-linked oligo-(dT) primers, and (6) repeating steps 3–5 to achieve the desired cDNA or aRNA amount for the library preparation.作者: grudging 時間: 2025-3-31 05:04
Fair Value of a Convertible Bond,h-quality data, including valid sequence information, rigorous quality assessment is also needed after library construction (i.e., prior to and during the use of a library for downstream applications). Applying a set of common quality criteria to newly generated cDNA libraries will become increasingly important (.).作者: ABYSS 時間: 2025-3-31 11:45
Islamophobia and the Talking Heads,onship to the populationary ratio of each RNA species (.). It has also been tested to generate a full-length mRNA library from as few as 20 homologous tissue cells (2-pg mRNAs) for profiling cancer stages in vivo.作者: Apogee 時間: 2025-3-31 15:26 作者: 性行為放縱者 時間: 2025-3-31 20:32 作者: dyspareunia 時間: 2025-4-1 00:25 作者: chiropractor 時間: 2025-4-1 05:01
cDNA Library Construction Using In Vitro Transcriptional Amplification,he promoter element of the cDNA to synthesize the aRNA sequence up to 2000-fold increase, (4) reverse transcription of the aRNA, (5) denaturation and then double-stranding the resulting cDNA with promoter-linked oligo-(dT) primers, and (6) repeating steps 3–5 to achieve the desired cDNA or aRNA amount for the library preparation.作者: delegate 時間: 2025-4-1 07:39
Quality Assessment of cDNA Libraries,h-quality data, including valid sequence information, rigorous quality assessment is also needed after library construction (i.e., prior to and during the use of a library for downstream applications). Applying a set of common quality criteria to newly generated cDNA libraries will become increasingly important (.).
