標題: Titlebook: Gene Transfer and Expression Protocols; E. J. Murray Book 1991 Springer Science+Business Media New York 1991 [打印本頁] 作者: Withdrawal 時間: 2025-3-21 18:02
書目名稱Gene Transfer and Expression Protocols影響因子(影響力)
書目名稱Gene Transfer and Expression Protocols影響因子(影響力)學科排名
書目名稱Gene Transfer and Expression Protocols網絡公開度
書目名稱Gene Transfer and Expression Protocols網絡公開度學科排名
書目名稱Gene Transfer and Expression Protocols被引頻次
書目名稱Gene Transfer and Expression Protocols被引頻次學科排名
書目名稱Gene Transfer and Expression Protocols年度引用
書目名稱Gene Transfer and Expression Protocols年度引用學科排名
書目名稱Gene Transfer and Expression Protocols讀者反饋
書目名稱Gene Transfer and Expression Protocols讀者反饋學科排名
作者: BIBLE 時間: 2025-3-21 23:09
Clinical Applications of Medical Imagingtein (.–.). Recombinants have been used to generate HIV-neutralizing antibody (.–.) to immunize animals and humans (.–.), and to analyze cytotoxic T-cell and antibody-dependent cellular cytotoxicity (.–.). Furthermore, the antigenicity of HIV .has been improved by site-directed mutagenesis of protease cleavage sites (.).作者: Ventricle 時間: 2025-3-22 00:57 作者: Talkative 時間: 2025-3-22 04:56
Manipulation of Vaccinia Virus Vectors,tein (.–.). Recombinants have been used to generate HIV-neutralizing antibody (.–.) to immunize animals and humans (.–.), and to analyze cytotoxic T-cell and antibody-dependent cellular cytotoxicity (.–.). Furthermore, the antigenicity of HIV .has been improved by site-directed mutagenesis of protease cleavage sites (.).作者: Original 時間: 2025-3-22 12:06
1064-3745 proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been作者: 制定 時間: 2025-3-22 15:44
The Clinical Aspects of Memory Disorders,ssion, and to facilitate the identification of genes participating in diverse cellular processes (namely, metabolism, the immune response, differentiation and development, the repair of DNA damage, and malignant transformation).作者: 制定 時間: 2025-3-22 20:23
Paolo G. Camici,Ornella Rimoldiing by somatic cell genetics (.). This method for constructing physical maps has been central to human genetics and has resulted in the chromosomal assignment of several hundred genes (..). Unfortunately, subchromosomal gene localization by this approach is limited by the paucity of suitable translocation and deletion chromosomes.作者: archaeology 時間: 2025-3-22 22:25 作者: Adherent 時間: 2025-3-23 02:58
Diagnosis and Treatment of Hyperthyroidismon cycle in permissive cells. The first step in the replication cycle is synthesis of early mRNA. This is followed by DNA replication and expression of late mRNAs that encode the structural proteins. New virus particles are formed, and the infection ultimately kills the cells.作者: 音樂戲劇 時間: 2025-3-23 08:37
Book 1991ms to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more作者: 態(tài)學 時間: 2025-3-23 10:33 作者: 破譯 時間: 2025-3-23 17:08
Irradiation and Fusion Gene Transfer,ing by somatic cell genetics (.). This method for constructing physical maps has been central to human genetics and has resulted in the chromosomal assignment of several hundred genes (..). Unfortunately, subchromosomal gene localization by this approach is limited by the paucity of suitable translocation and deletion chromosomes.作者: 帶傷害 時間: 2025-3-23 20:33 作者: Grievance 時間: 2025-3-24 01:15 作者: Canyon 時間: 2025-3-24 05:24 作者: stress-response 時間: 2025-3-24 06:54 作者: GONG 時間: 2025-3-24 13:17 作者: Communal 時間: 2025-3-24 18:17
Jos W. M. van der Meer,Bart Jan Kullberg wild-type cell line, one had first to select a recessive mutant derivative cell line and characterize it before proceeding with the experiments of interest. Such restrictions posed a significant barrier to molecular genetic analyses in higher eukaryotic cells.作者: 奇思怪想 時間: 2025-3-24 19:04
Transfection of the ChloramphenicolAcetyltransferase Gene into Eukaryotic Cells Using Diethyl-Aminoe level falls as the plasmid DNA is diluted out in a growing population of cells. The general strategy of subcloning putative regulatory regions followed by transfection and quantification of CAT activity is outlined in a flow diagram (.)作者: 誘騙 時間: 2025-3-25 00:43
,Cationic Liposome-Mediated Transfection with Lipofectin? Reagent,date large macromolecules, such as DNA or RNA, resulting in low capturing efficiency. In addition, conventional liposomal methodology involves a relatively inconvenient multistep preparation procedure.作者: 勤勞 時間: 2025-3-25 03:27
Choice and Manipulation of Retroviral Vectors,in tissue-culture experiments. In comparison, chemical and electrical means of gene transfer require large initial numbers of cells and, under optimal circumstances, function at efficiencies several orders of magnitude lower than retroviral vectors, and only in a limited number of specific cell types.作者: Negligible 時間: 2025-3-25 10:48
Use of Vectors to Confer Resistance to Antibiotics G418 and Hygromycin in Stably Transfected Cell L wild-type cell line, one had first to select a recessive mutant derivative cell line and characterize it before proceeding with the experiments of interest. Such restrictions posed a significant barrier to molecular genetic analyses in higher eukaryotic cells.作者: 幼稚 時間: 2025-3-25 12:57 作者: 帶傷害 時間: 2025-3-25 19:38 作者: Bravura 時間: 2025-3-25 22:17 作者: LITHE 時間: 2025-3-26 01:21
Physics and Technology of SPECT/CT,s or cells in culture, and analyze the levels of gene product produced by the introduced construct. Ideally, such a reporter gene should encode a product that is stable, innocuous to the cell or organism in which it is being expressed, and should be readily detectable, even when present in small quantities.作者: Lymphocyte 時間: 2025-3-26 07:32 作者: Alcove 時間: 2025-3-26 09:14
Manipulation of Baculovirus Vectors,w to the field. However, before describing the methodology peculiar to baculovirus expression vectors, it is of value to briefly consider the isolation, structure, and replication strategy of this unique group of viruses. Detailed and more comprehensive reviews that describe the biology of baculoviruses are available (.–.)作者: 外面 時間: 2025-3-26 13:19 作者: Explicate 時間: 2025-3-26 17:44
,Use of , (β-Galactosidase) as a Reporter Gene,s or cells in culture, and analyze the levels of gene product produced by the introduced construct. Ideally, such a reporter gene should encode a product that is stable, innocuous to the cell or organism in which it is being expressed, and should be readily detectable, even when present in small quantities.作者: Project 時間: 2025-3-26 21:39
978-1-4899-4403-0Springer Science+Business Media New York 1991作者: outer-ear 時間: 2025-3-27 03:23
Gene Transfer and Expression Protocols978-1-59259-494-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 反對 時間: 2025-3-27 05:39 作者: Oversee 時間: 2025-3-27 12:18
Manipulation of Adenovirus Vectors,e 43 of them human. The human adenoviruses, particularly types 2, 5, and 12, have been the most extensively characterized, and these viruses have served as valuable tools in the study of the molecular biology of DNA replication, transcription, RNA processing, and protein synthesis in mammalian cells. .. 1 for a general review.作者: Demonstrate 時間: 2025-3-27 17:12 作者: 非秘密 時間: 2025-3-27 21:26
Perineal Skin Care Considerations,imply, a way of getting DNA into a cell; and second, a reporter system, which, as the name suggests, is a means of finding out what the transfected DNA does from its new location inside the cell. These two requirements are amply met by transfecting cells, in the presence of the polycation diethyl am作者: 侵蝕 時間: 2025-3-27 23:32 作者: 蒼白 時間: 2025-3-28 02:10 作者: Pantry 時間: 2025-3-28 07:34 作者: Alcove 時間: 2025-3-28 14:27
The Practice of Cognitive Therapym other mammalian cells, and also the study of the function and the regulation of gene expression of mammalian genes in vivo. Various transfection techniques have been developed: incubation of recipient cells with genes in the presence of diethylaminoethyldextran (.., this vol.; ref. .); incubation 作者: 命令變成大炮 時間: 2025-3-28 17:53 作者: bibliophile 時間: 2025-3-28 20:18 作者: 模仿 時間: 2025-3-29 02:21
https://doi.org/10.1007/978-1-4615-5013-6n cells. This technique, termed chromosome-mediated gene transfer (CMGT), involves the transfer of subchromosomal fragments from the cells of one species (the donor, usually human) to those of another (the recipient, usually a rodent) that have the ability to maintain them. CMGT thus fills a gap bet作者: ARCH 時間: 2025-3-29 05:13
Akira Hashizume,Naruhito Hironagae 43 of them human. The human adenoviruses, particularly types 2, 5, and 12, have been the most extensively characterized, and these viruses have served as valuable tools in the study of the molecular biology of DNA replication, transcription, RNA processing, and protein synthesis in mammalian cells作者: Living-Will 時間: 2025-3-29 07:51
Clinical Applications of Medical Imaging >50 different virus gene products (for reviews, .refs. .). The widespread use of vaccinia virus as a vector owes much to the ease of generating recombinants, the authenticity of the foreign gene product, and the wide variety of uses to which they have been put. For example, HIV 1 (human immunodefic作者: 撫慰 時間: 2025-3-29 12:13
https://doi.org/10.1007/978-1-4613-1573-5sizing foreign gene products in a heterologous system. In contrast to the other recent excellent reviews on the subject (.,.), this contribution aims to explore the various practical aspects of the system, and in consequence, to highlight particular difficulties that may be encountered by workers ne作者: SIT 時間: 2025-3-29 19:34
Diagnosis and Treatment of Hyperthyroidismled DNA molecule of 5243 bp (.) that is packaged into a capsid containing the viral structural proteins VP1,VP2, and VP3. SV40 induces a lytic infection cycle in permissive cells. The first step in the replication cycle is synthesis of early mRNA. This is followed by DNA replication and expression o作者: 慎重 時間: 2025-3-29 22:09
RET and Some Mid-Life Problems,panded the capabilities of investigators to perform important experiments that have solved previously unanswerable biological questions. Retroviral vectors exploit the inherent capacity of retrovkuses to transfer genetic material stably into a cell’s genome and subsequently express it in a manner th作者: Abnormal 時間: 2025-3-30 01:48
I-MIBG SPECT/CT for Tumour Imaging, use of reporter genes. In essence, a reporter gene encodes a protein product for which a sensitive and convenient assay is available. Transcription of the reporter is driven by the control sequences of interest, so that the level of reporter protein reflects the transcriptional activity, Reporter g作者: 時代錯誤 時間: 2025-3-30 06:44
Physics and Technology of SPECT/CT,nto heterologous cellular systems both in vitro and in vivo. One approach to defining DNA sequences important in the regulation of gene expression is to place controlling elements (e.g., promoter/enhancer sequences) upstream of a DNA coding sequence, introduce these constructs into transgenic animal作者: BOLT 時間: 2025-3-30 09:54 作者: CLAIM 時間: 2025-3-30 16:13 作者: Merited 時間: 2025-3-30 17:05 作者: 放逐某人 時間: 2025-3-30 22:52
Calcium Phosphate Mediated Gene Transfer into Established Cell Lines,DNA transfection is one of the most important techniques in molecular genetics. It is this technique that has made possible the dissection of complex eukaryotic genes and the characterization of the function of their components (.–.) as well as the isolation of particular genes on the basis of their expression in cultured cells (.–.).作者: Cardiac-Output 時間: 2025-3-31 04:26 作者: Arteriography 時間: 2025-3-31 08:07
Transfection of the ChloramphenicolAcetyltransferase Gene into Eukaryotic Cells Using Diethyl-Aminoimply, a way of getting DNA into a cell; and second, a reporter system, which, as the name suggests, is a means of finding out what the transfected DNA does from its new location inside the cell. These two requirements are amply met by transfecting cells, in the presence of the polycation diethyl am作者: 發(fā)怨言 時間: 2025-3-31 12:07
Polybrene/DMSOAssisted Gene Transfer, displayed by the recipient cells toward the gene-transfer regimen itself. As a result, several laboratories have turned to the use of synthetic polycations for delivering copious amounts of exogenous DNA to target cells without compromising their viability or clonogenicity. One of these compounds, 作者: 種類 時間: 2025-3-31 14:27
Electroporation Technique of DNA Transfection,on of cells and DNA placed between electrodes in a suitable cuvet. It is thought that this pulse induces local areas of cell-membrane breakdown, or pores, through which the DNA then enters the cell. Once these pores have resealed, normal cell functions can continue. Since this transfection method in作者: MOTIF 時間: 2025-3-31 18:28 作者: Antagonist 時間: 2025-3-31 22:02
,Direct Use of λ Phage Particles for DNA Transfection,m other mammalian cells, and also the study of the function and the regulation of gene expression of mammalian genes in vivo. Various transfection techniques have been developed: incubation of recipient cells with genes in the presence of diethylaminoethyldextran (.., this vol.; ref. .); incubation 作者: BET 時間: 2025-4-1 05:47 作者: START 時間: 2025-4-1 09:32
Preparation of High-Molecular-Weight DNA for Use in DNA Transfection,eory, for any phenotype for which there is a suitable selection in cell culture, a gene sequence encoding that phenotype can be transferred and will complement the selection. If the donor sequences are derived from a different species of origin, the presence of the donor DNA in the recipient cell ca作者: 背帶 時間: 2025-4-1 10:17
Chromosome-Mediated Gene Thnsfer,n cells. This technique, termed chromosome-mediated gene transfer (CMGT), involves the transfer of subchromosomal fragments from the cells of one species (the donor, usually human) to those of another (the recipient, usually a rodent) that have the ability to maintain them. CMGT thus fills a gap bet作者: 連接 時間: 2025-4-1 14:47
