標(biāo)題: Titlebook: Gene Therapy Protocols; Paul D. Robbins Book 19971st edition Springer Science+Business Media New York 1997 [打印本頁(yè)] 作者: 難受 時(shí)間: 2025-3-21 19:02
書目名稱Gene Therapy Protocols影響因子(影響力)
作者: Breach 時(shí)間: 2025-3-21 22:03
Methods for Gene Transfer Using DNA-Adenovirus Conjugates,ors have been derived. These vector agents consist of two linked functional domains: a DNA-binding domain to transport the DNA as part of the vector complex, and a ligand domain to target a cellular receptor that allows entry of the conjugate-DNA complex into a receptor-mediated endocytosis pathway.作者: 混合,攙雜 時(shí)間: 2025-3-22 04:26
Development of Replication-Defective Herpes Simplex Virus Vectors,NS disease will require new delivery strategies and vehicles including the development of novel vectors for direct gene transfer. These vectors should: efficiently deliver the therapeutic gene(s) to a sufficient number of nondividing neurons; persist long-term in a nonintegrated state within the ner作者: 花束 時(shí)間: 2025-3-22 07:11 作者: 背心 時(shí)間: 2025-3-22 09:07
Methods for Adenovirus-Mediated Gene Transfer to Airway Epithelium,hase I clinical trails are now underway evaluating the safety of recombinant adenoviral vectors for gene therapy of cystic fibrosis lung disease (.). This clinical application of these vectors in CF lung disease presents the largest comprehensive effort of in vivo gene therapy to date. Such a substa作者: 改正 時(shí)間: 2025-3-22 16:47
Methods for Efficient Retrovirus-Mediated Gene Transfer to Mouse Hematopoietic Stem Cells,ethods for stem cell transduction that are effective with mouse cells have only been partially successful in dog, nonhuman primate, and human models. Whereas scale-up of stem cell transduction procedures for human applications will present unique technical problems, mouse models may yet provide furt作者: 改正 時(shí)間: 2025-3-22 17:30
1543-1894 es, it is difficult for scientists to perform all methods required for gene delivery and subsequent in vivo gene expression. This Gene Therapy Protocols volume 978-1-59259-591-4Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: 衰老 時(shí)間: 2025-3-22 23:29
Analyses of Processing Contamination,ors have been derived. These vector agents consist of two linked functional domains: a DNA-binding domain to transport the DNA as part of the vector complex, and a ligand domain to target a cellular receptor that allows entry of the conjugate-DNA complex into a receptor-mediated endocytosis pathway.作者: Champion 時(shí)間: 2025-3-23 04:32 作者: 問(wèn)到了燒瓶 時(shí)間: 2025-3-23 08:17 作者: NEX 時(shí)間: 2025-3-23 13:30 作者: 反復(fù)無(wú)常 時(shí)間: 2025-3-23 14:06 作者: MULTI 時(shí)間: 2025-3-23 18:05 作者: neuron 時(shí)間: 2025-3-24 01:30
John P. Houghton,Andrew Stevensive way of delivering genes into mammalian cells. This chapter aims to explore the various practical aspects of the AAV vector system, and in consequence, to highlight particular difficulties that may be encountered by workers new to the field. However, before describing the methodology involved in 作者: happiness 時(shí)間: 2025-3-24 05:06
https://doi.org/10.1057/9781137387967ewed in ref. .). One serious drawback of this technique, however, has been the difficulty in readily producing high-titer recombinant retroviruses. For many applications, such as infecting rare target cells or the majority of cells in tissue culture, the recombinant virus titer must be at least 10. 作者: Lament 時(shí)間: 2025-3-24 10:25 作者: DEFT 時(shí)間: 2025-3-24 14:22 作者: 充滿人 時(shí)間: 2025-3-24 15:09 作者: 出處 時(shí)間: 2025-3-24 22:47 作者: 四牛在彎曲 時(shí)間: 2025-3-25 02:11
https://doi.org/10.1007/978-3-030-10847-2iently infect mammalian cells, the potential risks of viral gene delivery spurred research in the development of synthetic chemical vectors that would duplicate viral delivery but have no risk of infectious complications. These nonviral vectors were first developed in the late 1980s. Cationic lipids作者: homeostasis 時(shí)間: 2025-3-25 04:59
https://doi.org/10.1007/978-981-13-7892-8plore and develop gene therapy strategies (.-.). Reported approaches to gene therapy mclude the uses of retroviruses (.,.), adenovirus (.,.), receptor-mediated endocytosis (.,.), direct injection (.), and liposomes (.,.), among others. Targeted delivery of DNA via receptors has been successfully app作者: 衰老 時(shí)間: 2025-3-25 09:03
Visions of the City. Introductioning the lungs, pancreas, intestine, salivary glands, and urogenital tract. The protein encoded by the CF gene is an integral plasma membrane protein called the cystic fibrosis transmembrane conductance regulator (CFTR) and has been shown to function as a chloride channel (.). In the lungs, CFTR dysf作者: 中國(guó)紀(jì)念碑 時(shí)間: 2025-3-25 15:43 作者: Irksome 時(shí)間: 2025-3-25 16:45
https://doi.org/10.1007/978-1-349-27923-4ty of applications include ex vivo and in vivo protocols in patients postnatally. Nonetheless there is increasing interest and compelling reasons to consider prenatal application of somatic gene therapy (.,.. In the current chapter, we will review theoretical, ethical, and experimental support for i作者: 無(wú)能力之人 時(shí)間: 2025-3-25 22:38
https://doi.org/10.1007/978-3-663-14659-9tening disorders resulting from a single genetic defect that do not compromise other liver functions and the organization of the hepatic tissue. Orthotopic liver transplantation has been successfully performed in patients with genetic liver disease, but then use is limited by the high mortality risk作者: Neonatal 時(shí)間: 2025-3-26 00:50 作者: 文藝 時(shí)間: 2025-3-26 07:14 作者: 赤字 時(shí)間: 2025-3-26 09:15 作者: 頭腦冷靜 時(shí)間: 2025-3-26 14:54 作者: 做作 時(shí)間: 2025-3-26 16:51
https://doi.org/10.1007/978-3-658-03786-4process termed self-renewal (.).The HSCs can be enriched from hematopoietic tissues using MAbs that bind to the CD34 antigen, a universally recognized marker for hematopoietic progenitors (.-.).Enriched HSC populations are being widely investigated for use in transplantation and gene therapy because作者: Synthesize 時(shí)間: 2025-3-26 21:40 作者: cluster 時(shí)間: 2025-3-27 02:04 作者: 合乎習(xí)俗 時(shí)間: 2025-3-27 08:54
Gene Therapy Protocols978-1-59259-591-4Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: backdrop 時(shí)間: 2025-3-27 09:26
https://doi.org/10.1007/978-3-663-14660-5 are advantageous for intravascular injection of virus into either portal or systemic circulation. The portal vein provides a direct iv route into the liver. It also theoretically provides an indirect route by oral administration since the portal system drains the gut.作者: 金桌活畫面 時(shí)間: 2025-3-27 17:35
https://doi.org/10.1007/978-3-322-99450-9o obtain high-titer vector stocks and the narrow host cell range of these vectors. To overcome these disadvantages, we developed a new class of pantropic retroviral vector that has a broadened host cell range and can be concentrated to very high titers (>10. colony forming units [CFU]/mL) (.)作者: Airtight 時(shí)間: 2025-3-27 20:09 作者: GLUT 時(shí)間: 2025-3-28 00:22 作者: 整體 時(shí)間: 2025-3-28 04:20 作者: CRATE 時(shí)間: 2025-3-28 09:43
Getting Lean — Die Verschlankung des Staatesate for the transduction of primary lymphocytes, because gene transfer is mediated by nonimmunogenic vectors and stable vector integration in the target genome is achieved. Stable integration in cells undergoing clonal expansion ensures that the foreign genetic material will be faithfully transmitted to the cells’ progeny.作者: 只有 時(shí)間: 2025-3-28 12:07
https://doi.org/10.1007/978-3-658-03786-4 marker for hematopoietic progenitors (.-.).Enriched HSC populations are being widely investigated for use in transplantation and gene therapy because they appear to provide rapid hematopoietic reconstitution in myeloablated patients (.-.), and they offer good targets for gene transfer (.-.).作者: 彩色的蠟筆 時(shí)間: 2025-3-28 15:56 作者: 華而不實(shí) 時(shí)間: 2025-3-28 20:28
Methods for Retrovirus-Mediated Gene Transfer into Primary T-Lymphocytes,ate for the transduction of primary lymphocytes, because gene transfer is mediated by nonimmunogenic vectors and stable vector integration in the target genome is achieved. Stable integration in cells undergoing clonal expansion ensures that the foreign genetic material will be faithfully transmitted to the cells’ progeny.作者: Postulate 時(shí)間: 2025-3-29 01:54 作者: Acupressure 時(shí)間: 2025-3-29 03:12 作者: 重力 時(shí)間: 2025-3-29 10:07 作者: Counteract 時(shí)間: 2025-3-29 11:33
https://doi.org/10.1007/978-3-658-26645-5us types of cells/tissues have been successfully genetically modified and are potential targets for retroviral mediated gene therapy (.).The successful use of these tissues in gene therapy relies on methods to cultivate and transplant the cells after genetic modification.作者: 完成才會(huì)征服 時(shí)間: 2025-3-29 17:24
Methods for the Construction of Retroviral Vectors and the Generation of High-Titer Producers,re when the foreign genetic material must be faithfully transmitted to the progeny of the transduced parental cell, murine leukemia virus (MLV)-based vectors accommodate numerous modifications, thus providing a plastic tool that can be tailored for very diverse applications.作者: thrombus 時(shí)間: 2025-3-29 21:12
Methods for the Use of Retroviral Vectors for Transfer of the CFTR Gene to Airway Epithelium,oride secretion lead to dehydration of the fluids on the airway surface and, in turn, this leads to chronic infections and severe damage. The severity of CF lung disease and the potential accessibility of the airways to gene transfer vectors has led to proposals that gene therapy be applied for the treatment of CF lung disease (.).作者: Ledger 時(shí)間: 2025-3-30 02:51
Methods for the Use of Genetically Modified Keratinocytes in Gene Therapy,us types of cells/tissues have been successfully genetically modified and are potential targets for retroviral mediated gene therapy (.).The successful use of these tissues in gene therapy relies on methods to cultivate and transplant the cells after genetic modification.作者: 語(yǔ)源學(xué) 時(shí)間: 2025-3-30 04:03
John P. Houghton,Andrew Stevensthe generation of recombinant AAV vectors, it is of value to briefly discuss the structure and life cycle of this unique virus. Detailed and more extensive reviews that describe the biology of adeno-associated virus are also available (.-.).作者: Foolproof 時(shí)間: 2025-3-30 09:17 作者: 逃避系列單詞 時(shí)間: 2025-3-30 15:39 作者: exclamation 時(shí)間: 2025-3-30 17:31
https://doi.org/10.1007/978-3-030-10847-2 are attractive vectors for vascular gene transfer studies owing to ease of preparation, reproducibility, and safety (.-.). In this chapter, we describe our experience with vascular gene transfer using four liposome reagents: DOTMA/DOPE, DC-cholesterol, DOSPA/DOPE, and DMRIE/DOPE.作者: cunning 時(shí)間: 2025-3-30 20:58
https://doi.org/10.1007/978-981-13-7892-8lied using protein ligands to the hepatic asialoglycoprotein receptor (ASGr) (.,.-.), and, subsequently, the transferrin receptor (.). The ASGr is a cell-surface receptor that is highly represented on hepatocytes. Thus, genes targeted to this receptor can be delivered in a highly selective manner to the liver.作者: Allure 時(shí)間: 2025-3-31 01:36
https://doi.org/10.1007/978-1-349-27923-4n utero gene therapy and then outline the methodology and large animal model we are currently using to consider retrovirus-mediated gene transfer to fetal lung. In this latter regard, the candidate inherited disorder is cystic fibrosis and the reader is referred to Chapters 1 and 12 in this volume.作者: inspired 時(shí)間: 2025-3-31 07:39
https://doi.org/10.1007/978-3-663-14659-9, the need for chronic immunosuppression, and the availability of organs. Gene therapy would pro vide an alternative to transplantation by restoring the expression of the defective gene in an organ that is otherwise structurally and functionally normal.作者: 叢林 時(shí)間: 2025-3-31 10:36
Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors,the generation of recombinant AAV vectors, it is of value to briefly discuss the structure and life cycle of this unique virus. Detailed and more extensive reviews that describe the biology of adeno-associated virus are also available (.-.).作者: 真繁榮 時(shí)間: 2025-3-31 14:05
Generation of High-Titer, Helper-Free Retroviruses by Transient Transfection,infectious units/mL. Although one can usually obtain high-titer mixtures of recombinant and replication-competent retroviruses in a relatively short time, many applications such as cell marking studies or studying genes in vivo demand freedom from replication-competent virus.作者: 加花粗鄙人 時(shí)間: 2025-3-31 20:57
Methods for the Use of Poliovirus Vectors for Gene Delivery,sequence of poliovirus has been determined (.), and the cellular receptor that pohovirus uses to enter cells has been cloned and sequenced (.). The availability of a transgenic mouse expressing the poliovirus receptor has facilitated further description of the pathogenesis of poliovirus (.,.).作者: IDEAS 時(shí)間: 2025-3-31 23:39
Methods for Liposome-Mediated Gene Transfer to the Arterial Wall, are attractive vectors for vascular gene transfer studies owing to ease of preparation, reproducibility, and safety (.-.). In this chapter, we describe our experience with vascular gene transfer using four liposome reagents: DOTMA/DOPE, DC-cholesterol, DOSPA/DOPE, and DMRIE/DOPE.作者: 不合 時(shí)間: 2025-4-1 05:36
Methods for Targeted Gene Transfer to Liver Using DNA-Protein Complexes,lied using protein ligands to the hepatic asialoglycoprotein receptor (ASGr) (.,.-.), and, subsequently, the transferrin receptor (.). The ASGr is a cell-surface receptor that is highly represented on hepatocytes. Thus, genes targeted to this receptor can be delivered in a highly selective manner to the liver.作者: 虛弱的神經(jīng) 時(shí)間: 2025-4-1 08:09
Methods for Retrovirus-Mediated Gene Transfer to Fetal Lung,n utero gene therapy and then outline the methodology and large animal model we are currently using to consider retrovirus-mediated gene transfer to fetal lung. In this latter regard, the candidate inherited disorder is cystic fibrosis and the reader is referred to Chapters 1 and 12 in this volume.作者: vector 時(shí)間: 2025-4-1 13:30
, Retrovirus-Mediated Gene Transfer into the Liver,, the need for chronic immunosuppression, and the availability of organs. Gene therapy would pro vide an alternative to transplantation by restoring the expression of the defective gene in an organ that is otherwise structurally and functionally normal.作者: 凌辱 時(shí)間: 2025-4-1 15:25
Methods for Gene Transfer Using DNA-Adenovirus Conjugates,sess endogenous pathways for internalization of macromolecules, the utilization of these pathways for the purpose of DNA delivery represents a strategy that potentially allows certain practical advantages. In this regard, these cellular internalization pathways can be highly efficient. For example, 作者: 光亮 時(shí)間: 2025-4-1 22:18
Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors,ive way of delivering genes into mammalian cells. This chapter aims to explore the various practical aspects of the AAV vector system, and in consequence, to highlight particular difficulties that may be encountered by workers new to the field. However, before describing the methodology involved in
