標(biāo)題: Titlebook: Gene Targeting Protocols; Eric B. Kmiec Book 2000 Springer Science+Business Media New York 2000 [打印本頁] 作者: Buren 時間: 2025-3-21 19:34
書目名稱Gene Targeting Protocols影響因子(影響力)
作者: chandel 時間: 2025-3-21 21:33
,Old Age and Alzheimer’s Disease in Film,oses, including adeno-associated virus, herpesvirus, and others (.,.). Each virus has advantages and disadvantages: The latter include inactivation by human complement, limited infectivity, low titers, short-lived expression, immunogenicity, contamination by replication-competent or helper virus, and so on.作者: 報復(fù) 時間: 2025-3-22 03:06
Konzeptioneller Rahmen und Forschungsstand,ction of the treated cells will acquire the designed change. The overall inefficiency may result from the rarity of precise homologous recombination and from the low frequency of appropriate gene transfer.作者: 緊張過度 時間: 2025-3-22 04:48
G. A. S. Premakumara,W. P. K. M. Abeysekeraally into the host chromosome (ch). Targeting of AAV to ch19.13.3 qter locus has been documented at a frequency of 70% or greater (.). Because of AAV’s unique ability to integrate site-specifically, extensive research has been devoted to understanding how AAV integrates, with the hope of creating targeting vectors for gene therapy.作者: Spinous-Process 時間: 2025-3-22 09:26
A. Dalhoff,S. Matutat,U. Ullmannhe analysis of the promoter and enhancer DNA elements, and of the corresponding DNA-binding proteins, has provided an understanding of the basic mechanisms of transcription initiation (.). This information is now used to devise gene expression cassettes for gene therapy.作者: 懦夫 時間: 2025-3-22 16:15 作者: 懦夫 時間: 2025-3-22 20:19
https://doi.org/10.1007/978-3-030-72147-3)is a technique that has shown promise in this application (.). SFHR has been used to correct the most common mutation associated with cystic fibrosis (CF), a 3-bp deletion in the CF transmembrane conductance regulator .gene, resulting in the loss of a phenylalanine at amino acid position 508 (Δ508) (.–.).作者: MERIT 時間: 2025-3-22 23:34 作者: BOGUS 時間: 2025-3-23 03:21
Book 2000ation, the cloned gene is returned to its homologous location in the genome and becomes inserted at the target locus. This process is a controlled means for the repair of DNA damage and ensures accurate chromosome disjunction during meiosis. The paradigm for thinking about the mechanism of this p- c作者: 歡呼 時間: 2025-3-23 07:46
Effective Gene Transfer Using Viral Vectors Based on SV40,oses, including adeno-associated virus, herpesvirus, and others (.,.). Each virus has advantages and disadvantages: The latter include inactivation by human complement, limited infectivity, low titers, short-lived expression, immunogenicity, contamination by replication-competent or helper virus, and so on.作者: expansive 時間: 2025-3-23 13:44
Mutation Correction by Homologous Recombination with an Adenovirus Vector,ction of the treated cells will acquire the designed change. The overall inefficiency may result from the rarity of precise homologous recombination and from the low frequency of appropriate gene transfer.作者: CODE 時間: 2025-3-23 13:57
Site-Specific Targeting of DNA Plasmids to Chromosome 19 Using AAV , and , Sequences,ally into the host chromosome (ch). Targeting of AAV to ch19.13.3 qter locus has been documented at a frequency of 70% or greater (.). Because of AAV’s unique ability to integrate site-specifically, extensive research has been devoted to understanding how AAV integrates, with the hope of creating targeting vectors for gene therapy.作者: 加強防衛(wèi) 時間: 2025-3-23 18:00 作者: 被詛咒的人 時間: 2025-3-23 22:23
Gene Targeting in Plants via Site-Directed Mutagenesis,tion genes are an unavoidable consequence of conventional transformation technologies. Reported herein are strategies to create, and select for, modifications to plant genes in vivo without the introduction of foreign DNA.作者: Hla461 時間: 2025-3-24 05:07
,Foundation III—Thought Experiments,enes might occur as a result of random integration. Therefore, significant interest has emerged to develop site-specific integration and precise integration technologies for future gene therapy protocols.作者: Ejaculate 時間: 2025-3-24 09:16
https://doi.org/10.1007/978-3-030-89140-4s, and metabolic storage diseases, as well as localized delivery to treat dystrophinopathies and cardiovascular disorders (.–.). Numerous studies have already demonstrated expression in muscle of such transgenes as various reporter enzymes and relevant therapeutic proteins (.,.–.,.–.).作者: Noisome 時間: 2025-3-24 13:16 作者: emission 時間: 2025-3-24 18:26
Danielle Goldfarb,Katherine M. Sharkeyerent strategies for recognizing DNA can be directly compared. The ability to detect and quantify an early, perhaps rate limiting step, in genetic targeting should be useful in efforts to better understand and improve this important technique.作者: calamity 時間: 2025-3-24 20:00 作者: 帶來 時間: 2025-3-25 00:23
Adeno-Associated Virus Based Gene Therapy in Skeletal Muscle,s, and metabolic storage diseases, as well as localized delivery to treat dystrophinopathies and cardiovascular disorders (.–.). Numerous studies have already demonstrated expression in muscle of such transgenes as various reporter enzymes and relevant therapeutic proteins (.,.–.,.–.).作者: 終點 時間: 2025-3-25 03:35
Rapid Establishment of Myeloma Cell Lines Expressing Fab(Tac)-Protamine, a Targetable Protein Vecto-mediated endocytosis, resulting in selective killing of the target cells. However, the low level of Fab(105)-protamine fusion protein secreted from the stably transduced COS cells has been a limiting factor for experiments requiring large amounts of the fusion protein.作者: 歹徒 時間: 2025-3-25 09:18
Use of Quantitative Ligation-Mediated Polymerase Chain Reaction to Detect Gene Targeting by Alkylaterent strategies for recognizing DNA can be directly compared. The ability to detect and quantify an early, perhaps rate limiting step, in genetic targeting should be useful in efforts to better understand and improve this important technique.作者: fertilizer 時間: 2025-3-25 15:08
Eric B. KmiecIncludes supplementary material: 作者: 欄桿 時間: 2025-3-25 18:38
978-1-4899-4158-9Springer Science+Business Media New York 2000作者: 植物群 時間: 2025-3-25 20:31
Gene Targeting Protocols978-1-59259-215-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: immunity 時間: 2025-3-26 03:18
Kumiko Ito-Miwa,Kazuki Terauchi,Takao KondoAntisense oligonucleotides have been extensively used as downregulators of gene expression (.), and as such are not only increasingly used in various fields as sequence-specific research tools (.–.), but are also tested in clinical trials as antiviral and anticancer agents (.–.).作者: Aids209 時間: 2025-3-26 05:01 作者: braggadocio 時間: 2025-3-26 09:14
https://doi.org/10.1007/978-3-030-70590-9n recent years. The principal reasons for this have been the ease with which the methodology can be applied to a wide range of cell types; the relatively low cytotoxicity compared to other techniques; the high efficiency of nucleic acid transfer in comparison with methods such as calcium phosphate o作者: Thyroiditis 時間: 2025-3-26 15:33 作者: 類似思想 時間: 2025-3-26 18:27 作者: 笨拙處理 時間: 2025-3-26 22:52
,Foundation III—Thought Experiments,human clinical trials has been to produce long-term expression of therapeutic genes by integration of the DNA sequences encoding these genes into the chromosomes. Integration strategies include random, site-specific, and precise integration. To date, only random integration of retroviral vectors has作者: 統(tǒng)治人類 時間: 2025-3-27 02:18 作者: cardiac-arrest 時間: 2025-3-27 06:06 作者: chassis 時間: 2025-3-27 11:10 作者: 移植 時間: 2025-3-27 16:22 作者: Vaginismus 時間: 2025-3-27 18:11 作者: 時代 時間: 2025-3-27 23:41
https://doi.org/10.1007/978-3-030-89140-4ired diseases, with over 100 protocols approved by the National Institutes of Health since 1989. Skeletal muscle is an especially attractive target for gene therapy, because of its accessibility and capability to uptake, maintain, and express recombinant protein from plasmid DNA (.–.). Transduction 作者: Benign 時間: 2025-3-28 03:53 作者: PIZZA 時間: 2025-3-28 10:07
A. Dalhoff,S. Matutat,U. Ullmannon initiation. Research in the past two decades has provided a wealth of information about the mechanisms of transcriptional control in human cells. The analysis of the promoter and enhancer DNA elements, and of the corresponding DNA-binding proteins, has provided an understanding of the basic mecha作者: Terrace 時間: 2025-3-28 13:53 作者: Finasteride 時間: 2025-3-28 18:02
Danielle Goldfarb,Katherine M. Sharkeylkylate complex genomic DNA under physiological conditions. The alkylating event, which is detected using a quantitative version of the ligation-mediated polymerase chain reaction (LM-PCR) (.,.), acts as a marker for complex formation, and permits an estimation of the gene targeting frequency. Becau作者: 令人悲傷 時間: 2025-3-28 20:58
https://doi.org/10.1007/978-3-030-20051-0in transgenic plants. These genes provide a means to select plant cells which have acquired the linked foreign gene that encodes a useful trait; selection genes are an unavoidable consequence of conventional transformation technologies. Reported herein are strategies to create, and select for, modif作者: Limousine 時間: 2025-3-28 22:56
Cinematic Perspectives on Digital Culture molecular medicine, that is, gene therapy and intracellular immunization. Among the major steps involved in the modification of target cells for these applications, gene transfer and the delivery of molecular medicine has become more and more critical.作者: 難聽的聲音 時間: 2025-3-29 04:19 作者: arabesque 時間: 2025-3-29 08:46
Gene Transfer and Drug Delivery by Electronic Pulse Delivery, molecular medicine, that is, gene therapy and intracellular immunization. Among the major steps involved in the modification of target cells for these applications, gene transfer and the delivery of molecular medicine has become more and more critical.作者: 臆斷 時間: 2025-3-29 12:11 作者: 使厭惡 時間: 2025-3-29 17:56
Nucleic Acid Transfer Using Cationic Lipids,n recent years. The principal reasons for this have been the ease with which the methodology can be applied to a wide range of cell types; the relatively low cytotoxicity compared to other techniques; the high efficiency of nucleic acid transfer in comparison with methods such as calcium phosphate o作者: 完整 時間: 2025-3-29 21:54 作者: beta-cells 時間: 2025-3-29 23:57
Gene Transfer and Drug Delivery by Electronic Pulse Delivery, molecular medicine, that is, gene therapy and intracellular immunization. Among the major steps involved in the modification of target cells for these applications, gene transfer and the delivery of molecular medicine has become more and more critical.作者: 尊重 時間: 2025-3-30 04:35
Strategies for Improving the Frequency and Assessment of Homologous Recombination,human clinical trials has been to produce long-term expression of therapeutic genes by integration of the DNA sequences encoding these genes into the chromosomes. Integration strategies include random, site-specific, and precise integration. To date, only random integration of retroviral vectors has作者: 鄙視 時間: 2025-3-30 09:19 作者: BAN 時間: 2025-3-30 14:53 作者: 有助于 時間: 2025-3-30 20:37
Site-Directed Alteration of Genomic DNA by Small-Fragment Homologous Replacement,(.)is a technology that can be used to achieve a number of different ends, including:the introduction of specific mutations into vectors, development of transgenic animals, and gene therapy.Homologous replacement is versatile, in that sequences can be directly targeted and altered, inserted, or dele作者: 流利圓滑 時間: 2025-3-30 20:55
Mutation Correction by Homologous Recombination with an Adenovirus Vector,lian systems (.). Its wider application, especially to human gene therapy, is, however, hampered by its low level of efficiency. Only a very small fraction of the treated cells will acquire the designed change. The overall inefficiency may result from the rarity of precise homologous recombination a作者: 名字 時間: 2025-3-31 02:21
Site-Specific Targeting of DNA Plasmids to Chromosome 19 Using AAV , and , Sequences,d helper virus (i.e., adenovirus [Ad] or herpesvirus) to undergo productive infection. In the absence of a helper virus, AAV will integrate preferentially into the host chromosome (ch). Targeting of AAV to ch19.13.3 qter locus has been documented at a frequency of 70% or greater (.). Because of AAV’作者: menopause 時間: 2025-3-31 05:49
Adeno-Associated Virus Based Gene Therapy in Skeletal Muscle,ired diseases, with over 100 protocols approved by the National Institutes of Health since 1989. Skeletal muscle is an especially attractive target for gene therapy, because of its accessibility and capability to uptake, maintain, and express recombinant protein from plasmid DNA (.–.). Transduction 作者: 反抗者 時間: 2025-3-31 12:05 作者: 匍匐 時間: 2025-3-31 14:49
EBV-Derived Episomes to Probe Chromatin Structure and Gene Expression in Human Cells,on initiation. Research in the past two decades has provided a wealth of information about the mechanisms of transcriptional control in human cells. The analysis of the promoter and enhancer DNA elements, and of the corresponding DNA-binding proteins, has provided an understanding of the basic mecha
