標題: Titlebook: Gene Knockout Protocols; Wolfgang Wurst,Ralf Kühn Book 2009Latest edition Humana Press 2009 DNA.ES cells.Gene modification.Genetically eng [打印本頁] 作者: burgeon 時間: 2025-3-21 18:02
書目名稱Gene Knockout Protocols影響因子(影響力)
書目名稱Gene Knockout Protocols影響因子(影響力)學科排名
書目名稱Gene Knockout Protocols網(wǎng)絡公開度
書目名稱Gene Knockout Protocols網(wǎng)絡公開度學科排名
書目名稱Gene Knockout Protocols被引頻次
書目名稱Gene Knockout Protocols被引頻次學科排名
書目名稱Gene Knockout Protocols年度引用
書目名稱Gene Knockout Protocols年度引用學科排名
書目名稱Gene Knockout Protocols讀者反饋
書目名稱Gene Knockout Protocols讀者反饋學科排名
作者: 亞當心理陰影 時間: 2025-3-21 21:45
Construction of Gene-Targeting Vectors by Recombineeringof knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors.作者: 透明 時間: 2025-3-22 02:47
Gene-Trap Vectors and Mutagenesish-throughput use, in an effort to inactivate all genes in the mouse genome. Gene trapping is performed with vectors that simultaneously inactivate and report the expression of the trapped gene and provide a molecular tag for its rapid identification. Gene-trap approaches have been used successfully 作者: 運動的我 時間: 2025-3-22 05:19
Chromosome Engineering in ES Cellsr phenotypically neutral. To understand the genetic consequences of such genomic changes, these mutations need to be modelled in experimentally tractable systems. The mouse is an excellent organism for this analysis because of its biological and genetic similarity to humans, the ease with which its 作者: ingenue 時間: 2025-3-22 11:22 作者: 憤憤不平 時間: 2025-3-22 16:17
Generation of shRNA Transgenic Miceeasy manner. For the use of RNAi in mice, short hairpin (sh) RNAs expressed stably from the genome are a faster alternative to conventional knockout approaches. Here, we describe an advanced strategy for complete or conditional gene knockdown in mice, where the Cre/loxP system is used to activate RN作者: 憤憤不平 時間: 2025-3-22 19:30 作者: geometrician 時間: 2025-3-22 23:18
Gene Targeting in Mouse Embryonic Stem Cellsr ability to alter the mouse genome has been limited by both the lack of technologies to conditionally target a locus and by conventional cloning. The “cre/loxP” and “recombineering” technologies have overcome some of these limitations and have greatly enhanced our ability to manipulate the mouse ge作者: 鉤針織物 時間: 2025-3-23 05:20 作者: patriot 時間: 2025-3-23 08:27 作者: Finasteride 時間: 2025-3-23 12:46 作者: 評論者 時間: 2025-3-23 15:36
Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro type of the organism. Here we describe basic protocols for the in vitro differentiation of mouse ES cells into cells of the cardiac, neuronal, pancreatic, and hepatic lineage. The protocols include (1) the formation of embryoid bodies (EBs) followed by (2) the spontaneous differentiation of EBs int作者: 神經(jīng) 時間: 2025-3-23 21:17
Cloning of ES Cells and Mice by Nuclear Transferiezo-actuated micromanipulator. Although the piezo unit is a complex tool, once mastered it is of great help not only in NT experiments, but also in almost all other forms of micromanipulation. Using this technique, embryonic stem (ntES) cell lines established from somatic cell nuclei can be generat作者: 幻想 時間: 2025-3-24 01:01 作者: 小隔間 時間: 2025-3-24 05:05
Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryosrom undifferentiated cell populations to fully committed functional cell types. This chapter gives a description of the early events of cell commitment and differentiation in the pre-and postimplantation-stage embryo. Next, a discussion follows highlighting the most commonly used as well as more rec作者: 掃興 時間: 2025-3-24 07:40
VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Er equally high-throughput methods for the production of mice for phenotypic studies. In response to this challenge, we recently developed a new method termed VelociMouse for the production of F0-generation mice that are fully derived from gene-targeted ES cells. In the version of the VelociMouse met作者: 時間等 時間: 2025-3-24 13:23
Book 2009Latest editiong of their molecular interaction network. The mouse offers many advantages for the use of genetics to study human biology and disease, unmatched among other m- mals. Its development, body plan, physiology, behavior, and diseases have much in common, based on the fact that 99% of the human genes have作者: 值得贊賞 時間: 2025-3-24 18:40 作者: 商店街 時間: 2025-3-24 21:31 作者: 致敬 時間: 2025-3-25 01:23 作者: 厚顏 時間: 2025-3-25 05:32 作者: Obvious 時間: 2025-3-25 08:56 作者: nauseate 時間: 2025-3-25 14:50
Manipulating Mouse Embryonic Stem Cellsy after extensive in vitro manipulations. Here we provide straightforward protocols for proper care of these cells. Special emphasis is placed on aspects that are particularly critical for proper culture of this cell type.作者: 庇護 時間: 2025-3-25 16:38 作者: 交響樂 時間: 2025-3-25 20:23
Cloning of ES Cells and Mice by Nuclear Transfered relatively easily from a variety of mouse genotypes and cell types. Such ntES cells can be used not only for experimental models of human therapeutic cloning but also as a means of preserving mouse genomes instead of preserving germ cells. Here, we describe our most recent protocols for mouse cloning.作者: 秘方藥 時間: 2025-3-26 03:46 作者: 不透明性 時間: 2025-3-26 05:06
Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryosently developed applications of various cell types and origins used in the production of chimeras. Finally, detailed protocols and trouble-shooting suggestions will be presented for each of the steps involved.作者: 量被毀壞 時間: 2025-3-26 10:11 作者: DIS 時間: 2025-3-26 15:21 作者: persistence 時間: 2025-3-26 20:07 作者: agnostic 時間: 2025-3-26 23:37
king mice. Presently, approximately 70% of the protein-coding genes in the mouse genome have been disrupted by gene-trap insertions. Here we describe the basic methodology used to induce and characterize gene-trap mutations in ESCs.作者: Conflagration 時間: 2025-3-27 02:53
Levels of Analysis: The Inter-state Leveluse genome. The resulting mouse models are leading to a better understanding of the molecular and cellular basis of dosage alterations in human disease phenotypes, in turn opening new diagnostic and therapeutic opportunities.作者: 平庸的人或物 時間: 2025-3-27 08:20 作者: 五行打油詩 時間: 2025-3-27 12:14 作者: ESO 時間: 2025-3-27 15:11
Gene-Trap Vectors and Mutagenesisking mice. Presently, approximately 70% of the protein-coding genes in the mouse genome have been disrupted by gene-trap insertions. Here we describe the basic methodology used to induce and characterize gene-trap mutations in ESCs.作者: Aggressive 時間: 2025-3-27 21:25 作者: Agnosia 時間: 2025-3-27 22:25 作者: 愛好 時間: 2025-3-28 02:52 作者: constitute 時間: 2025-3-28 09:07 作者: 打算 時間: 2025-3-28 14:25
eeding to render potential recessive mutations homozygous, at which time phenotype screens can be performed. An alternative strategy for randomly mutagenizing the mouse genome is by chemical treatment of ES cells. This enables the use of multiple alternative chemicals with different mutational spect作者: 記成螞蟻 時間: 2025-3-28 15:58
Free-Spirit Education: What It Means,ctively. The differentiated cells show tissue-specific proteins and electrophysiological properties (action potentials and ion channels) in cardiac and neuronal cells, glucose-dependent insulin release in pancreatic cells, or glycogen storage and albumin synthesis in hepatic cells. The protocols pre作者: 預測 時間: 2025-3-28 19:25 作者: 閑逛 時間: 2025-3-29 00:37
Mutagenesis of Mouse Embryonic Stem Cells with Ethylmethanesulfonateeeding to render potential recessive mutations homozygous, at which time phenotype screens can be performed. An alternative strategy for randomly mutagenizing the mouse genome is by chemical treatment of ES cells. This enables the use of multiple alternative chemicals with different mutational spect作者: 獎牌 時間: 2025-3-29 06:50 作者: 有毒 時間: 2025-3-29 09:30
VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Eh inbred or hybrid ES cells and either inbred or outbred eight-cell host embryos. Because the F0 mice produced are suitable for direct phenotyping studies, the VelociMouse method, coupled with high-throughput ES cell targeting technologies, such as VelociGene, offers an accelerated path to new drug 作者: 饑荒 時間: 2025-3-29 11:54
Book 2009Latest editionility of the genome sequence supports gene-driven approaches such as gene-trap and targeted mutagenesis in ES cells, allowing efficient and precise gene disruption. In combination with the use of site-specific DNA recombinases, in particular the Cre/loxP system, gene disruptioncan be directed to spe作者: 恩惠 時間: 2025-3-29 19:17
Redistributing Risks and Responsibilities,velopments and advice for choosing a mutagenesis strategy. Where appropriate, reference is given to relevant chapters of this book, key original articles and links of web-based resources for mouse mutagenesis.作者: –FER 時間: 2025-3-29 20:22
Fenglian Du,Wenbin Wang,Xiaoyuan Dongof knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors.作者: Benzodiazepines 時間: 2025-3-30 03:22
https://doi.org/10.1007/978-981-99-5227-4hodology for isolation and culture but also to the validation of freshly derived lines, in order to be maintained for prolonged time without significant differentiation or karyotype instability, and to provide reproducible germline transmission in chimaeric mice.作者: 悅耳 時間: 2025-3-30 06:48 作者: 蚊子 時間: 2025-3-30 10:03 作者: Allodynia 時間: 2025-3-30 12:22 作者: ALIEN 時間: 2025-3-30 17:20 作者: TRUST 時間: 2025-3-30 20:41 作者: 送秋波 時間: 2025-3-31 04:37
h-throughput use, in an effort to inactivate all genes in the mouse genome. Gene trapping is performed with vectors that simultaneously inactivate and report the expression of the trapped gene and provide a molecular tag for its rapid identification. Gene-trap approaches have been used successfully 作者: Ebct207 時間: 2025-3-31 05:52
Levels of Analysis: The Inter-state Levelr phenotypically neutral. To understand the genetic consequences of such genomic changes, these mutations need to be modelled in experimentally tractable systems. The mouse is an excellent organism for this analysis because of its biological and genetic similarity to humans, the ease with which its 作者: agenda 時間: 2025-3-31 10:12
https://doi.org/10.1007/978-94-017-1031-2lls. However, oligonucleotide-directed substitution, insertion or deletion of a single or a few nucleotides was hampered by DNA mismatch repair (MMR). We have developed strategies to circumvent this problem based on findings that the central MMR protein MSH2 acts in two different mismatch recognitio作者: 我沒有命令 時間: 2025-3-31 13:30 作者: 得意人 時間: 2025-3-31 20:29
hnologies to manipulate the genome, plus its developmental and physiological similarities to humans, it has become a goal to generate mutations in all mouse genes and analyze the phenotypic consequences. Gene targeting in embryonic stem (ES) cells is the method of choice for making null mutations in作者: 痛恨 時間: 2025-4-1 00:25 作者: pulmonary 時間: 2025-4-1 02:23
Promotion Model of Chinese Rule of Law Path,mal gametes following in vitro genetic manipulations. This remarkable characteristic of ES cells has provided the basis for studying normal gene function in the mouse by targeted mutagenesis. Nevertheless, ES cells are very sensitive and need to be manipulated with care for them to retain totipotenc
