派博傳思國際中心

標(biāo)題: Titlebook: Gene Knockout Protocols; Martin J. Tymms,Ismail Kola Book 2001 Humana Press 2001 [打印本頁]

作者: invigorating    時(shí)間: 2025-3-21 17:21
書目名稱Gene Knockout Protocols影響因子(影響力)




書目名稱Gene Knockout Protocols影響因子(影響力)學(xué)科排名




書目名稱Gene Knockout Protocols網(wǎng)絡(luò)公開度




書目名稱Gene Knockout Protocols網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Gene Knockout Protocols被引頻次




書目名稱Gene Knockout Protocols被引頻次學(xué)科排名




書目名稱Gene Knockout Protocols年度引用




書目名稱Gene Knockout Protocols年度引用學(xué)科排名




書目名稱Gene Knockout Protocols讀者反饋




書目名稱Gene Knockout Protocols讀者反饋學(xué)科排名





作者: ARK    時(shí)間: 2025-3-21 23:09
How to Study Pathologic Phenotypes of Knockout Mice,. Sometimes breeding the mutated gene onto another genetic background by repeated backcrossings (i.e., making a new congenic strain) brings out a phenotype, as many genes are now known to be or are suspected to be modified by other genes.
作者: 執(zhí)    時(shí)間: 2025-3-22 04:11
Analysis of Hematopoietic Phenotypes in Knockout Mouse Models,dinated expression of many genes that directly or indirectly govern hematopoietic stem and progenitor cell maintenance, maturation, lineage commitment, and differentiation; cell migration, mobilization, and sequestration; and mature blood cell function (.–.).
作者: 祖先    時(shí)間: 2025-3-22 04:47
Influence of Genetic Background on Knockout Mouse Phenotypes,e targeted allele is placed can cause considerable variation in phenotype. This variation can present itself as completely different phenotypes, as variations in penetrance of phenotype, or as variable expressivity of phenotype.
作者: osculate    時(shí)間: 2025-3-22 08:50

作者: 鑒賞家    時(shí)間: 2025-3-22 14:36
etraploid embryo derived cells have a complementary distribution in chimeras made between them. In such chimeras, the embryo proper, the amnion, the yolk sac mesoderm, the allantois and the chorionic mesoderm-derived part of the placenta are completely ES cell-derived, whereas the yolk sac endoderm
作者: 鑒賞家    時(shí)間: 2025-3-22 18:24

作者: glowing    時(shí)間: 2025-3-22 21:34
Corrupt Seed: The Tainted Progenydinated expression of many genes that directly or indirectly govern hematopoietic stem and progenitor cell maintenance, maturation, lineage commitment, and differentiation; cell migration, mobilization, and sequestration; and mature blood cell function (.–.).
作者: 無所不知    時(shí)間: 2025-3-23 05:12

作者: 反感    時(shí)間: 2025-3-23 07:37

作者: 冰雹    時(shí)間: 2025-3-23 11:05

作者: 意見一致    時(shí)間: 2025-3-23 14:43
1064-3745 ogenous DNA occurred at a f- quency sufficient for recombinants to be isolated. In the next few years the mouse genome will be systematically altered, and the techniques for achi- ing manipulat978-1-61737-080-9978-1-59259-220-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
作者: pacifist    時(shí)間: 2025-3-23 19:14

作者: flaunt    時(shí)間: 2025-3-23 22:25

作者: 有害處    時(shí)間: 2025-3-24 04:34
,State Intervention, 1901–1904, in gene expression, the functional diversity of cell types formed is increased, but the spectrum of lineage potentials for any given progenitor cell is diminished. The processes of lineage commitment and differentiation are regulated by an array of soluble cytokines (.) and growth factors (.–.) and by intercellular interactions (.).
作者: Friction    時(shí)間: 2025-3-24 09:12
Creation and Use of a Cre Recombinase Transgenic Database,knockout” resulted in early embryonic lethality, which obscured the study of potential later functions. In other cases the “knockout” did not have any phenotype due to the compensation of the gene deficiency by other family members. These limitations have called for further development of the powerful gene-targeting technology (.–.).
作者: exclamation    時(shí)間: 2025-3-24 13:37
Isolation of Embryonic Fibroblasts and Their Use in the In Vitro Characterization of Gene Function, for pups to be born. Also, if the gene modification results in lethality late (i.e., >d 12) in the fetal period, then an analysis of the “phenotype” or of gene function could still be undertaken at the cell biological or molecular level using the embryonic fibroblast cells.
作者: Mucosa    時(shí)間: 2025-3-24 17:11
Embryonic Stem Cells in the Study of Hematopoiesis, in gene expression, the functional diversity of cell types formed is increased, but the spectrum of lineage potentials for any given progenitor cell is diminished. The processes of lineage commitment and differentiation are regulated by an array of soluble cytokines (.) and growth factors (.–.) and by intercellular interactions (.).
作者: Irrepressible    時(shí)間: 2025-3-24 19:55

作者: 機(jī)密    時(shí)間: 2025-3-25 02:07
train of mice for mating contribute to the genetic composition of the targeted mouse, whereas the strain of host embryos is only relevant in that it must be compatible with the choice of ES cells, otherwise the ability of the chimeric mice to provide germline transmission of the ES cell genome may be compromised.
作者: 大范圍流行    時(shí)間: 2025-3-25 04:44

作者: 充滿人    時(shí)間: 2025-3-25 10:26
Choice of Mouse Strains for Gene Targeting,train of mice for mating contribute to the genetic composition of the targeted mouse, whereas the strain of host embryos is only relevant in that it must be compatible with the choice of ES cells, otherwise the ability of the chimeric mice to provide germline transmission of the ES cell genome may be compromised.
作者: Conscientious    時(shí)間: 2025-3-25 12:15

作者: Intact    時(shí)間: 2025-3-25 16:11
of development. This system can also be used to facilitate transgene activation/inactivation in vivo, deletions of large stretches of genomic DNA, chromosomal translocation and subtle alterations to genes and/or their regulatory sequences in vivo.
作者: 緩解    時(shí)間: 2025-3-25 22:06

作者: 帶來墨水    時(shí)間: 2025-3-26 00:53

作者: Mobile    時(shí)間: 2025-3-26 04:43
Classification of Multiword Expressions,). Gain-of-function mutations are generally generated by cDNA overexpression and in this chapter we review the status of heterologous gene expression systems that can be used for this purpose in ES cells.
作者: 疏遠(yuǎn)天際    時(shí)間: 2025-3-26 09:45

作者: 天真    時(shí)間: 2025-3-26 16:43

作者: GENUS    時(shí)間: 2025-3-26 18:04

作者: Culpable    時(shí)間: 2025-3-26 22:30

作者: 門閂    時(shí)間: 2025-3-27 01:06
Interferon-Inducible ES Cell Expression Systems,). Gain-of-function mutations are generally generated by cDNA overexpression and in this chapter we review the status of heterologous gene expression systems that can be used for this purpose in ES cells.
作者: 繁殖    時(shí)間: 2025-3-27 08:10

作者: 散布    時(shí)間: 2025-3-27 09:27
Book 2001e ultimate goal: to understand the normal biological functions of these genes, and how alterations lead to disease states. In this task there is a severe limitation in working with human material, but the mouse has been adopted as the favored animal model because of the available genetic resources a
作者: erythema    時(shí)間: 2025-3-27 16:52
Graeme Johanson,Anja Michaela FladrichEmbryonic stem (ES) cells, the totipotent cells of early embryos established as permanent lines (.,.) retain their developmental capacity in vivo (.) as well as in vitro (..–.). The totipotent properties of ES cells are the basis of the gene-targeting technology to create mutant mice strains with inactivated genes by homologous recombination (.).
作者: constitute    時(shí)間: 2025-3-27 20:03

作者: 最高點(diǎn)    時(shí)間: 2025-3-27 22:45
f gene products. Not only can gene disruption demonstrate the function of genes, but by disrupting cell development or survival it can also demonstrate the function of specific cell types. Some knockout mice are murine models of human genetic diseases and have proven that a single gene defect is cap
作者: 微塵    時(shí)間: 2025-3-28 05:53

作者: 600    時(shí)間: 2025-3-28 09:50
ansmitted through the mouse germline (.–.), the application of a variety of gene-targeting methodologies has generated a remarkable number of novel and informative mutant mouse strains that have been invaluable not only for the study of gene function in vivo, but also serving as model systems for hu
作者: set598    時(shí)間: 2025-3-28 13:31

作者: inferno    時(shí)間: 2025-3-28 18:28

作者: Jogging    時(shí)間: 2025-3-28 20:01

作者: 蜿蜒而流    時(shí)間: 2025-3-29 02:06
ted to date. Analysis of these genetic alterations has provided an unprecedented understanding of critical gene functions that underlie normal developmental and disease mechanisms in mammals. We have also learned, however, that mammalian genetic determination is complex: genes have multiple function
作者: 薄荷醇    時(shí)間: 2025-3-29 05:47

作者: gastritis    時(shí)間: 2025-3-29 09:00

作者: 擴(kuò)音器    時(shí)間: 2025-3-29 14:05
hniques and materials, including embryonic stem (ES) cells (.) and tetraploid embryos (.,.). ES cells are pluripotent cell lines derived from late blastocyst-stage embryos, which are capable of differentiating into all derivatives of the primitive ectoderm (..) when aggregated with or injected into
作者: duplicate    時(shí)間: 2025-3-29 16:56

作者: HILAR    時(shí)間: 2025-3-29 20:12

作者: 新義    時(shí)間: 2025-3-30 01:21

作者: judiciousness    時(shí)間: 2025-3-30 05:04

作者: 過份好問    時(shí)間: 2025-3-30 08:33
The Meaning of T’sip in Qing Lawtic processes that take place during gastrulation and early organogenesis. These experiments allow the analysis of the developmental fate and the differentiation potency of cells that are allocated to different tissue lineages during postimplantation development. Results of lineage analyses using .
作者: 吞沒    時(shí)間: 2025-3-30 14:44
ual cells and in the context of a complete organism. The method of gene inactivation relies on the ability of virtually every cell type to exchange DNA sequences with a high degree of sequence similarity by homologous recombination. In general, the procedure involves the generation of a targeting co
作者: 兒童    時(shí)間: 2025-3-30 19:39

作者: 發(fā)電機(jī)    時(shí)間: 2025-3-30 23:18

作者: 背書    時(shí)間: 2025-3-31 02:06

作者: Sigmoidoscopy    時(shí)間: 2025-3-31 05:45

作者: 擴(kuò)音器    時(shí)間: 2025-3-31 10:20

作者: 難聽的聲音    時(shí)間: 2025-3-31 14:56
enome and to generate mice with specific targeted gene mutations. The improvement in the quality of reagents (embryonic stem (ES) cells and media) available to the scientific community has made this technology accessible to many laboratories.
作者: fatuity    時(shí)間: 2025-3-31 17:51
re relatively straightforward and many of the experimental procedures are standardised (.–.). A further step toward increasing the efficiency in the generation of knockout mice lies in the pooling of resources and knowledge by establishing a centralized gene-targeting facility.
作者: yohimbine    時(shí)間: 2025-3-31 23:43

作者: 開始沒有    時(shí)間: 2025-4-1 05:07
Manipulating Mouse Embryonic Stem Cells,enome and to generate mice with specific targeted gene mutations. The improvement in the quality of reagents (embryonic stem (ES) cells and media) available to the scientific community has made this technology accessible to many laboratories.
作者: 孤獨(dú)無助    時(shí)間: 2025-4-1 08:54
Gene Targeting in a Centralized Facility,re relatively straightforward and many of the experimental procedures are standardised (.–.). A further step toward increasing the efficiency in the generation of knockout mice lies in the pooling of resources and knowledge by establishing a centralized gene-targeting facility.
作者: neutralize    時(shí)間: 2025-4-1 12:09
Overview,f gene products. Not only can gene disruption demonstrate the function of genes, but by disrupting cell development or survival it can also demonstrate the function of specific cell types. Some knockout mice are murine models of human genetic diseases and have proven that a single gene defect is cap
作者: 小木槌    時(shí)間: 2025-4-1 17:10
Isolation and Maintenance of Primate Embryonic Stem Cells,er particular conditions, these cells differentiate into derivatives of endoderm, mesoderm, ectoderm, and trophoblast (.,.). In mammals, many developmental events are studied using mouse embryos or ES cells, but some aspects of development differ significantly between humans and mice, such as the ti




歡迎光臨 派博傳思國際中心 (http://www.pjsxioz.cn/) Powered by Discuz! X3.5
卢龙县| 阳高县| 平潭县| 十堰市| 尚志市| 杂多县| 勐海县| 双峰县| 永登县| 岫岩| 鞍山市| 潜江市| 嘉黎县| 延川县| 和平区| 天气| 冀州市| 文安县| 楚雄市| 海丰县| 苏尼特右旗| 宁都县| 靖江市| 抚顺县| 喜德县| 界首市| 桦川县| 敦煌市| 弥渡县| 辽中县| 九江县| 禄劝| 城口县| 博乐市| 梁平县| 曲阳县| 尼玛县| 裕民县| 涿州市| 晋江市| 甘孜县|