標(biāo)題: Titlebook: Gene Isolation and Mapping Protocols; Jacqueline Boultwood Book 1997 Humana Press 1997 [打印本頁] 作者: 決絕 時(shí)間: 2025-3-21 17:34
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書目名稱Gene Isolation and Mapping Protocols讀者反饋
書目名稱Gene Isolation and Mapping Protocols讀者反饋學(xué)科排名
作者: 搬運(yùn)工 時(shí)間: 2025-3-21 23:13
d environmental influences). In general, the complexity arises when the simple correspondence between genotype and phenotype is not one to one due to possible misclassification of phenotype, incomplete and age-dependent penetrance, phenocopies, genetic heterogeneity and/or oligogenic inheritance. Er作者: 擴(kuò)張 時(shí)間: 2025-3-22 03:11 作者: 寬度 時(shí)間: 2025-3-22 06:03 作者: 奇思怪想 時(shí)間: 2025-3-22 12:09
LangLib: An Online English Study Communityrecognized that the ability to determine the location of genes and DNA sequences on human chromosomes would not only facilitate the identification of disease-related genes, but might also provide important knowledge on the organization of chromosomes and the mechanisms of gene expression. With the i作者: 同謀 時(shí)間: 2025-3-22 13:15 作者: 同謀 時(shí)間: 2025-3-22 17:08
Jürgen M. Gei?inger,Britta Vasters yeast. The considerable advances made in physically mapping these genomes has demanded the development of a technique capable of filling the niche between conventional Southern blotting and such approaches as somatic cell genetics and fluorescence . hybridization (FISH). Pulsed-field gel electropho作者: 管理員 時(shí)間: 2025-3-22 23:52
The Lineage in Society and State, no knowledge of the biological function of the molecule involved, relying only on genetic inheritance or chromosomal perturbation as keys to isolating the gene. The key step in the positional cloning of a disease-causing gene is the conversion of the mapping information into physically cloned DNA. 作者: 他很靈活 時(shí)間: 2025-3-23 03:42
Dexu He,Zhixiong Du,Jiechang Xial maps, for example, in . and . (.,.), was attempted using cosmid clones randomly selected and analyzed by restriction fingerprinting techniques (.). Cosmids were selected as the vector of choice owing to the ease of library construction and, at the time, the relatively large genomic insert size pos作者: 凝視 時(shí)間: 2025-3-23 06:41 作者: 節(jié)省 時(shí)間: 2025-3-23 11:00 作者: Obloquy 時(shí)間: 2025-3-23 17:28 作者: 星球的光亮度 時(shí)間: 2025-3-23 21:42
ysis of mutations in these genes. Direct selection is an expression-based gene identification technique that can rapidly identify cDNAs within large genomic regions. It has been successfully used in numerous positional cloning projects (.–.). It can capture cDNAs that are expressed, temporally and s作者: 返老還童 時(shí)間: 2025-3-24 00:22
lopment of an organism. This assumption is based on the fact that only a fraction of these genes, in the range of 10,000–15,000, are expressed in any cell type. The alterations in this process are therefore often the cause of developmental and pathological abnormalities, such as cancer.作者: 清真寺 時(shí)間: 2025-3-24 03:47 作者: 精確 時(shí)間: 2025-3-24 07:52
https://doi.org/10.1007/978-3-031-10580-7 differences will explain the nature of the variants. In practice, this is a daunting task in a genome the size of human (3000 Mb) and may take many years to achieve. Genome mapping and sequencing projects invert the problem by attempting the systematic discovery and ordering of all genes over a per作者: 吹牛需要藝術(shù) 時(shí)間: 2025-3-24 13:18 作者: ADJ 時(shí)間: 2025-3-24 18:51
Mingjiang Tu,Ling Li,Jiafu Zhenguracy of the method is dependent on the identification of a species difference between the human and rodent species. This chapter details methods for DNA-based gene mapping techniques using somatic cell hybrids.作者: originality 時(shí)間: 2025-3-24 19:42
Some Marginal Notes on the Poems of Po Chü-I.). Positional cloning of disease genes requires that previously uncharacterized transcripts be mapped to the smallest possible defined region. We have developed an efficient polymerase chain reaction (PCR)-based procedure for the rapid assignment of ESTs to human chromosome regions (.–.; .). The critical features of the method are:作者: CRUMB 時(shí)間: 2025-3-25 00:37 作者: Alcove 時(shí)間: 2025-3-25 06:19 作者: DUCE 時(shí)間: 2025-3-25 10:33 作者: 農(nóng)學(xué) 時(shí)間: 2025-3-25 14:20
Detection and Isolation of Differentially Expressed Genes by Differential Display,lopment of an organism. This assumption is based on the fact that only a fraction of these genes, in the range of 10,000–15,000, are expressed in any cell type. The alterations in this process are therefore often the cause of developmental and pathological abnormalities, such as cancer.作者: 感情 時(shí)間: 2025-3-25 18:55 作者: 運(yùn)動(dòng)吧 時(shí)間: 2025-3-25 22:01 作者: Heretical 時(shí)間: 2025-3-26 01:34
William Wei,Ilan Alon,Liqiang NiThere are currently a number of techniques available for the isolation of novel coding sequences from cloned genomic fragments. The most recent methodologies include exon trapping (.), direct selection (.,.), and screening of cDNA libraries with whole radiolabeled yeast artificial chromosomes (YACs) (YAC hybridization) (.).作者: ectropion 時(shí)間: 2025-3-26 07:29 作者: Foreshadow 時(shí)間: 2025-3-26 08:40
Isolation of cDNAs Using the YAC Hybridization Screen Method,There are currently a number of techniques available for the isolation of novel coding sequences from cloned genomic fragments. The most recent methodologies include exon trapping (.), direct selection (.,.), and screening of cDNA libraries with whole radiolabeled yeast artificial chromosomes (YACs) (YAC hybridization) (.).作者: ALIEN 時(shí)間: 2025-3-26 15:48
Software for Genetic Linkage Analysis,Reviews describing the software available to support genetic linkage analysis have appeared intermittently over the last few years (.,.). This period has been associated with several significant influences that have made it timely to reconsider the available programs and related computing resources. These influences are:作者: Afflict 時(shí)間: 2025-3-26 19:12
Jürgen M. Gei?inger,Britta Vasterstically karyotyping yeasts and protozoa, but with the advent of commercially available rare cutter restriction enzymes, the technique was soon used for the long-range physical mapping of bacterial, yeast, and eukaryotic genomes.作者: Narcissist 時(shí)間: 2025-3-26 22:39 作者: NIL 時(shí)間: 2025-3-27 03:15
Chapter Two Iconoclasm in Chinese Ibsenism,al exons (.–.) or entire 3′-terminal exons (.). The requirement of complete, intact exons as targets has proven absolutely essential, and only the trapping of complete internal or 3′-terminal exons is practical.作者: definition 時(shí)間: 2025-3-27 09:04
A Case Study: Translation of Legal Terms,y computer analysis of large genomic sequence (i.e., “software trapping”) is also becoming a viable alternative. After an overview of the various computational methods at hand, this chapter focuses on the use of database similarity searches for the identification of exons in mammalian genomes.作者: 可互換 時(shí)間: 2025-3-27 11:16
Gene Isolation by Exon Trapping,al exons (.–.) or entire 3′-terminal exons (.). The requirement of complete, intact exons as targets has proven absolutely essential, and only the trapping of complete internal or 3′-terminal exons is practical.作者: 背叛者 時(shí)間: 2025-3-27 15:47 作者: 朋黨派系 時(shí)間: 2025-3-27 20:22
Construction and Use of YAC Contigs in Disease Regions,g the gene. The key step in the positional cloning of a disease-causing gene is the conversion of the mapping information into physically cloned DNA. Advances in genomic technology, particularly in the construction, use, and accessibility of large-insert libraries, have greatly facilitated this process.作者: 通情達(dá)理 時(shí)間: 2025-3-27 23:08 作者: Euthyroid 時(shí)間: 2025-3-28 04:02 作者: 小丑 時(shí)間: 2025-3-28 09:54
The Lineage in Society and State,g the gene. The key step in the positional cloning of a disease-causing gene is the conversion of the mapping information into physically cloned DNA. Advances in genomic technology, particularly in the construction, use, and accessibility of large-insert libraries, have greatly facilitated this process.作者: 不理會(huì) 時(shí)間: 2025-3-28 11:30
Chinese Investment in Australia(.) and the fact that prior knowledge of the target gene sequence is required. It is for these reasons that the traditional cDNA library still has a valuable role to play in the isolation of any new gene.作者: 六個(gè)才偏離 時(shí)間: 2025-3-28 15:07
https://doi.org/10.1007/978-3-031-10580-7ears to achieve. Genome mapping and sequencing projects invert the problem by attempting the systematic discovery and ordering of all genes over a period of many years. We may reasonably expect a complete map and sequences of all the genes in the human genome (not the entire genome sequence) by about 2005.作者: 埋葬 時(shí)間: 2025-3-28 19:17
viation from independent assortment, but also a consistent recombination fraction between them. This recombination fraction is very roughly proportional to the physical distance between the two genes, and it is this principle that forms the basis of genetic linkage mapping.作者: surrogate 時(shí)間: 2025-3-29 02:18 作者: slipped-disk 時(shí)間: 2025-3-29 03:37 作者: Stress-Fracture 時(shí)間: 2025-3-29 08:04 作者: tolerance 時(shí)間: 2025-3-29 12:59 作者: Cumulus 時(shí)間: 2025-3-29 17:59
Gene Mapping Using Somatic Cell Hybrids,uracy of the method is dependent on the identification of a species difference between the human and rodent species. This chapter details methods for DNA-based gene mapping techniques using somatic cell hybrids.作者: 管理員 時(shí)間: 2025-3-29 20:20
Gene Mapping by FISH,recognized that the ability to determine the location of genes and DNA sequences on human chromosomes would not only facilitate the identification of disease-related genes, but might also provide important knowledge on the organization of chromosomes and the mechanisms of gene expression. With the i作者: 外貌 時(shí)間: 2025-3-30 01:10
Probe Ordering and Distancing by FISH,al context of the cell. The method involves hybridization of labeled probes to denatured target chromatin that has been fixed on microscopic slides, followed by visualization using standard (immuno) cytochemical procedures. Originally, probes were marked with radioisotopes and visualized by autoradi作者: Anonymous 時(shí)間: 2025-3-30 04:50
Physical Mapping by Pulsed-Field Gel Electrophoresis, yeast. The considerable advances made in physically mapping these genomes has demanded the development of a technique capable of filling the niche between conventional Southern blotting and such approaches as somatic cell genetics and fluorescence . hybridization (FISH). Pulsed-field gel electropho作者: 起草 時(shí)間: 2025-3-30 12:17 作者: disparage 時(shí)間: 2025-3-30 16:20 作者: ERUPT 時(shí)間: 2025-3-30 17:22
Use of Dinucleotide Polymorphism Analyses in Physical Mapping,ld be as frequent as one in approximately every 30–40 kb of the human genome. Thus, the entire genome can be represented by a large number of dC-dA or dG-dT repeat sequences. The repeat elements vary in size and can be analyzed by polymerase chain reaction (PCR) using unique primers; the high degree作者: circuit 時(shí)間: 2025-3-30 20:56
Mapping Expressed Sequence Tags (ESTs) by Multiplexing PCR Reactions from Hybrid Cell Panels and De.). Positional cloning of disease genes requires that previously uncharacterized transcripts be mapped to the smallest possible defined region. We have developed an efficient polymerase chain reaction (PCR)-based procedure for the rapid assignment of ESTs to human chromosome regions (.–.; .). The cr作者: Processes 時(shí)間: 2025-3-31 04:25 作者: BOON 時(shí)間: 2025-3-31 08:42
Isolation of Coding Sequences from Genomic Regions Using Direct Selection,ysis of mutations in these genes. Direct selection is an expression-based gene identification technique that can rapidly identify cDNAs within large genomic regions. It has been successfully used in numerous positional cloning projects (.–.). It can capture cDNAs that are expressed, temporally and s作者: 驚奇 時(shí)間: 2025-3-31 10:46 作者: placebo-effect 時(shí)間: 2025-3-31 15:56 作者: 宣傳 時(shí)間: 2025-3-31 21:12
Gene Mapping and Isolation, differences will explain the nature of the variants. In practice, this is a daunting task in a genome the size of human (3000 Mb) and may take many years to achieve. Genome mapping and sequencing projects invert the problem by attempting the systematic discovery and ordering of all genes over a per作者: 賭博 時(shí)間: 2025-4-1 00:11
