標(biāo)題: Titlebook: Gene Essentiality; Methods and Protocol Long Jason Lu Book 2015 Springer Science+Business Media New York 2015 Candida albicans.Computationa [打印本頁(yè)] 作者: 祈求 時(shí)間: 2025-3-21 17:22
書(shū)目名稱(chēng)Gene Essentiality影響因子(影響力)
作者: amputation 時(shí)間: 2025-3-21 22:53
Identifying Essential , Genes Using Genome-Wide Deletion Mutation,enes in the . SK36 strain using genome-wide deletion mutation. A fusion PCR-based method is used to construct gene deletion fragments, which contain kanamycin resistance cassettes with two flanking arms of DNA upstream and downstream of the target gene. The linear fused PCR amplicons were transforme作者: ARBOR 時(shí)間: 2025-3-22 02:14 作者: NUL 時(shí)間: 2025-3-22 06:21 作者: 純樸 時(shí)間: 2025-3-22 10:26 作者: GEAR 時(shí)間: 2025-3-22 13:12
Identifying Essential Genes in , by Global Phenotypic Profiling,libraries after pressure from selection. Although this method can be used for any organism in which transposon mutagenesis is possible, this chapter describes its use in .. More specifically, the methods for generating complex libraries through transposon mutagenesis, design of selective pressure, e作者: GEAR 時(shí)間: 2025-3-22 18:39
Essential Genes in the Infection Model of ,-PCR-Based Signature-Tagged Mutagenesis,tion of genes that will diminish or enhance maintenance of an organism in a specific ecological niche or environment. PCR-based STM applied to . permitted the identification of genes essential for in vivo maintenance by transposon insertion and negative selection in a mixed population of bacterial m作者: 愛(ài)了嗎 時(shí)間: 2025-3-23 00:14 作者: LAP 時(shí)間: 2025-3-23 02:04 作者: FLOUR 時(shí)間: 2025-3-23 06:38
A Statistical Framework for Improving Genomic Annotations of Transposon Mutagenesis (TM) Assigned Eiment to identify essential genes in Prokaryota. However, due to the limitation of high-throughput technique, this approach yields substantial systematic biases resulting in the incorrect assignments of many essential genes. To obtain unbiased and accurate annotations of essential genes from TM expe作者: GULP 時(shí)間: 2025-3-23 11:58
A Proposed Essential Gene Discovery Pipeline: A , Case Study,the genomics era, the “next-gen” genomic era provides vast amounts of genetic information. Sequencing of a representative bacterial pathogen genome has been superseded by sequencing of whole strain collections, whether from environmental or clinical sources (Harris et al., Science 327:469–474, 2010;作者: synovium 時(shí)間: 2025-3-23 14:55 作者: 神圣將軍 時(shí)間: 2025-3-23 18:04 作者: 圓木可阻礙 時(shí)間: 2025-3-24 01:23
Gene Essentiality Analysis Based on DEG 10, an Updated Database of Essential Genes,aea, and eukaryotes. DEG 10, the current release, includes not only essential protein-coding genes determined by genome-wide gene essentiality screens but also essential noncoding RNAs, promoters, regulatory sequences, and replication origins. Therefore, DEG 10 includes essential genomic elements un作者: 為敵 時(shí)間: 2025-3-24 04:28
Discovering Essential Domains in Essential Genes,s. We hypothesized that protein domains, the independent structural or functional units of a polypeptide chain, are responsible for gene essentiality. If the essentiality of domains is known, the essential genes could be identified. To find such essential domains, we have developed an EM algorithm-b作者: enumaerate 時(shí)間: 2025-3-24 09:31
Book 2015, in both prokaryotes and .Candida albicans.. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and correcti作者: Morbid 時(shí)間: 2025-3-24 12:08 作者: Panacea 時(shí)間: 2025-3-24 16:01
Kerry H. Robinson,Cristyn Daviesio single-gene deletion library, we undertook the development of the ASKA single-gene deletion library carrying a different antibiotic resistance. In addition, we developed tools for identification of synthetic lethal gene combinations by systematic construction of double-gene knockout mutants. We introduce these methods herein.作者: 領(lǐng)帶 時(shí)間: 2025-3-24 19:16
Caroline Stockman,Emma Nottinghamgenesis of pathogenic leptospires, identification of transposon insertion sites using direct sequencing from genomic DNA and a nested PCR utilizing degenerate oligonucleotides, and methods for testing mutant attenuation in the hamster model of infection.作者: laxative 時(shí)間: 2025-3-25 02:35 作者: Confound 時(shí)間: 2025-3-25 04:13
,The Great War over Children, 1914–1918,e to quickly define all the insertional mutants in a library and thus identify nonessential genes under the conditions in which the library was produced. Identification of fitness of individual mutants under specific conditions can be performed by exposing the library to selective pressures.作者: justify 時(shí)間: 2025-3-25 08:25 作者: 脖子 時(shí)間: 2025-3-25 11:49 作者: 圓錐體 時(shí)間: 2025-3-25 17:25 作者: 暗語(yǔ) 時(shí)間: 2025-3-25 23:10
Gene Essentiality Analysis Based on DEG 10, an Updated Database of Essential Genes,ntial genes in bacterial genomes exhibits an exponential decay with increasing genome sizes. The functions, ATP binding (GO:0005524), GTP binding (GO:0005525), and DNA-directed RNA polymerase activity (GO:0003899), are likely required for organisms across life domains.作者: 粗俗人 時(shí)間: 2025-3-26 02:50 作者: esoteric 時(shí)間: 2025-3-26 08:07
Microarray Transposon Tracking for the Mapping of Conditionally Essential Genes in ,,wth conditions. With a fully saturated library, the absence of transposon insertions can be used as an indicator of a gene essential for the survival and growth for the conditions used for the mutant library.作者: Individual 時(shí)間: 2025-3-26 09:44
Identification of Essential Genes and Synthetic Lethal Gene Combinations in , K-12,io single-gene deletion library, we undertook the development of the ASKA single-gene deletion library carrying a different antibiotic resistance. In addition, we developed tools for identification of synthetic lethal gene combinations by systematic construction of double-gene knockout mutants. We introduce these methods herein.作者: Palate 時(shí)間: 2025-3-26 13:36 作者: 陶器 時(shí)間: 2025-3-26 20:44
Children, their Families and the Lawxtraction of genomic DNA, amplification and quantification of transposon insertions through next-generation deep sequencing are covered. Determining gene essentiality and statistical analysis on data collected are also discussed.作者: Cosmopolitan 時(shí)間: 2025-3-27 00:14
The Partitive and the Part-Whole Schemes,ased Essential Domain Prediction (EDP) Model. With simulated datasets, the model provided convergent results given different initial values and offered accurate predictions even with noise. We then applied the EDP model to six microbes and predicted 3,450 domains to be essential in at least one species, ranging 8–24 % in each species.作者: 是貪求 時(shí)間: 2025-3-27 01:20
Identifying Essential Genes in , by Global Phenotypic Profiling,xtraction of genomic DNA, amplification and quantification of transposon insertions through next-generation deep sequencing are covered. Determining gene essentiality and statistical analysis on data collected are also discussed.作者: senile-dementia 時(shí)間: 2025-3-27 05:26
Discovering Essential Domains in Essential Genes,ased Essential Domain Prediction (EDP) Model. With simulated datasets, the model provided convergent results given different initial values and offered accurate predictions even with noise. We then applied the EDP model to six microbes and predicted 3,450 domains to be essential in at least one species, ranging 8–24 % in each species.作者: monochromatic 時(shí)間: 2025-3-27 09:55
Book 2015gents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and up-to-date, .Gene Essentiality: Methods and Protocols. will aid researchers who wish to further our knowledge in this vital field of study..作者: Conscientious 時(shí)間: 2025-3-27 17:15
Topics in Contemporary Semioticsized STM mutants coupled to metabolomics analysis can now be attempted systematically on a genomic scale. PCR-based STM and combined whole-genome methods can also be applied to any organism having selectable phenotypes for screening.作者: 懦夫 時(shí)間: 2025-3-27 18:21
https://doi.org/10.1007/978-1-4757-9682-7genesis for complex haploinsufficiency screening in ., indicating methods for transposon construction, mutagenesis, phenotypic screening, and identification of insertion sites in strains of interest. In total, the approach is a useful means to implement large-scale synthetic genetic screening in the作者: 捏造 時(shí)間: 2025-3-27 23:26
Internal Properties of the Scales,ystem. Using the selected features as input, we constructed an ensemble classifier and trained the model on a well-studied microorganism. After fine-tuning the model parameters in cross-validation, we tested the model on the other microorganism. We found that the tenfold cross-validation results wit作者: 神秘 時(shí)間: 2025-3-28 05:46 作者: bioavailability 時(shí)間: 2025-3-28 07:01 作者: 漂白 時(shí)間: 2025-3-28 11:05
scription of the procedure to predict the essentiality of metabolic genes with respect to a specific metabolic function. We exemplify the approach by predicting the essentiality of reactions in the citric acid cycle for the production of glucose from fatty acids.作者: 持久 時(shí)間: 2025-3-28 15:47 作者: laparoscopy 時(shí)間: 2025-3-28 18:59
1064-3745 g and avoiding known pitfalls..Authoritative and up-to-date, .Gene Essentiality: Methods and Protocols. will aid researchers who wish to further our knowledge in this vital field of study..978-1-4939-4862-8978-1-4939-2398-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: GIBE 時(shí)間: 2025-3-28 23:23 作者: assent 時(shí)間: 2025-3-29 06:35 作者: 溺愛(ài) 時(shí)間: 2025-3-29 11:16 作者: 縮影 時(shí)間: 2025-3-29 15:07
A Statistical Framework for Improving Genomic Annotations of Transposon Mutagenesis (TM) Assigned Entroducing a latent variable the real insertion number, we formalized the statistical framework. Through iteratively updating and optimizing model parameters to maximize the goodness-of-fit of the model to the observed TM insertion data, we finalized the model. Using this model, we are able to assig作者: debase 時(shí)間: 2025-3-29 18:58 作者: 飛鏢 時(shí)間: 2025-3-29 21:21 作者: 爵士樂(lè) 時(shí)間: 2025-3-30 03:48
Three Computational Tools for Predicting Bacterial Essential Genes, belong to the same family (for EGP) or phylum (for CEG_Match and Geptop) with one of the reference species, respectively. As the pilot software for the issue, predicting accuracies of them have been assessed and compared with existing algorithms, and note that all of other published algorithms have作者: Iniquitous 時(shí)間: 2025-3-30 06:29
978-1-4939-4862-8Springer Science+Business Media New York 2015作者: 性冷淡 時(shí)間: 2025-3-30 11:22 作者: Root494 時(shí)間: 2025-3-30 12:28 作者: CBC471 時(shí)間: 2025-3-30 20:33 作者: 流行 時(shí)間: 2025-3-31 00:25 作者: Amenable 時(shí)間: 2025-3-31 00:58
A Coastal Classroom without Walls,enes in the . SK36 strain using genome-wide deletion mutation. A fusion PCR-based method is used to construct gene deletion fragments, which contain kanamycin resistance cassettes with two flanking arms of DNA upstream and downstream of the target gene. The linear fused PCR amplicons were transforme
