標題: Titlebook: Gene Delivery to Mammalian Cells; Volume 1: Nonviral G William C. Heiser Book 2004 Humana Press 2004 DNA.Ethylen.Vivo.gene transfer.genes.l [打印本頁] 作者: 關(guān)稅 時間: 2025-3-21 17:57
書目名稱Gene Delivery to Mammalian Cells影響因子(影響力)
書目名稱Gene Delivery to Mammalian Cells影響因子(影響力)學(xué)科排名
書目名稱Gene Delivery to Mammalian Cells網(wǎng)絡(luò)公開度
書目名稱Gene Delivery to Mammalian Cells網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Gene Delivery to Mammalian Cells被引頻次
書目名稱Gene Delivery to Mammalian Cells被引頻次學(xué)科排名
書目名稱Gene Delivery to Mammalian Cells年度引用
書目名稱Gene Delivery to Mammalian Cells年度引用學(xué)科排名
書目名稱Gene Delivery to Mammalian Cells讀者反饋
書目名稱Gene Delivery to Mammalian Cells讀者反饋學(xué)科排名
作者: 里程碑 時間: 2025-3-21 20:21
Book 2004 used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo..Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers作者: caldron 時間: 2025-3-22 02:49
Chemical Methods for DNA Deliveryvivo. More importantly, in the last few years, we have witnessed significant efforts and progress in elucidating the mechanisms underlying synthetic vector-mediated DNA delivery. With the continuous effort to meet the need for safe and efficient gene-delivery methods for human gene therapy, it is fo作者: JADED 時間: 2025-3-22 05:39
DNA Delivery to Cells in Culture Using Peptidese directly with the cellular membranes. Although this chapter will discuss several methods for preparing peptide/DNA complexes, we will focus on the solid phase synthesis of peptides and the complexes that these peptides form with DNA.作者: progestin 時間: 2025-3-22 10:09 作者: Ferritin 時間: 2025-3-22 14:54 作者: Ferritin 時間: 2025-3-22 20:31 作者: Connotation 時間: 2025-3-22 21:46 作者: hypotension 時間: 2025-3-23 04:00 作者: CORD 時間: 2025-3-23 08:29
Carey DeMichelis,Michel Ferrarie directly with the cellular membranes. Although this chapter will discuss several methods for preparing peptide/DNA complexes, we will focus on the solid phase synthesis of peptides and the complexes that these peptides form with DNA.作者: 鳴叫 時間: 2025-3-23 12:39
Assessment and measurement of pain,o (.,.,.,.,.). The positively charged dendrimer-DNA complexes facilitate transfer of DNA into a cell primarily through endocytosis. DNA in a complexed form is protected from nuclease activity while the majority of the DNA template remains transcriptionaly active (.). The well-designed dendrimer-base作者: evasive 時間: 2025-3-23 14:32
Ghazaleh Rostami Nia MD,S. Abbas Shobeiri MDnviral delivery systems such as naked DNA injection, physical (gene gun or electroporation), and chemical (cationic liposome or polymer) approaches have been developed (.). In particular, since the discovery of its ability to complex and condense DNA and the subsequent demonstration of efficient DNA作者: exceed 時間: 2025-3-23 21:22
Kirsten Scheiwe,Harry Willekensr divided into chemical and physical methods. The purpose of this chapter is to provide a simple overview on the latter type. However, it is important to understand that such a distinction may not be very clear, and different methods can often be combined to achieve the maximum expression of transfe作者: Mundane 時間: 2025-3-24 02:15 作者: irritation 時間: 2025-3-24 02:45
Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextrane BES variation), has proven to be the only method sufficient for the cotransfection of multiple plasmids into a wide variety of cell types. Although not as widely used today, DEAE-dextran-mediated transfection is a highly reproducible method for transient expression of a foreign gene.作者: Exhilarate 時間: 2025-3-24 08:19
DNA Delivery to Cells in Culture Using PNA Clampsistance to degradation by nucleases and proteases and poor affinity for proteins that normally bind nucleic acids. For these reasons and others, PNA has been proposed for use as a therapeutic agent in controlling gene expression through either antisense or antigene activity.作者: habile 時間: 2025-3-24 10:58 作者: shrill 時間: 2025-3-24 16:45
1064-3745 py successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on 作者: 合群 時間: 2025-3-24 23:02 作者: 昏迷狀態(tài) 時間: 2025-3-25 01:41
https://doi.org/10.1007/978-1-4757-9673-5istance to degradation by nucleases and proteases and poor affinity for proteins that normally bind nucleic acids. For these reasons and others, PNA has been proposed for use as a therapeutic agent in controlling gene expression through either antisense or antigene activity.作者: 笨拙處理 時間: 2025-3-25 04:08 作者: Obsessed 時間: 2025-3-25 08:19 作者: AGONY 時間: 2025-3-25 12:03
Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexesand polycations (.,.) have all been reported as effective methods for intravascular gene delivery to the lung. Of the different nonviral approaches, DNA complexes with polyethyleneimine (PEI) have been reported to provide the most effective gene delivery to the lung.作者: pericardium 時間: 2025-3-25 19:17
M. Claire Greene,Jeremy C. Kane vectors has been directed toward a therapy for cystic fibrosis (.-.), other lung diseases, including asthma, chronic obstructive pulmonary disease, and cancer are being explored as viable disease targets.作者: Vulnerary 時間: 2025-3-25 21:28 作者: 翻動 時間: 2025-3-26 01:03 作者: Ballerina 時間: 2025-3-26 06:21 作者: 流眼淚 時間: 2025-3-26 09:10
https://doi.org/10.1057/9781137280671monstrated to react spontaneously with anionic DNA to form lipid-DNA complexes, or “l(fā)ipoplexes” (.). These lipoplexes demonstrated effective binding to cells in vitro and facilitation of intracellular delivery of a transgene. Felgner provides a detailed history of the progress leading up to and including lipofection (.).作者: scrape 時間: 2025-3-26 14:28
Permanent Versus Temporary ENS,ood flow through the pulmonary capillaries is slow and discontinuous. On inspiration, as the alveoli fill with air, blood flow slows and stops. On expiration, blood flow resumes. Slower blood flow in the lung favors DNA binding to the endothelial cells.作者: kyphoplasty 時間: 2025-3-26 17:28 作者: Kindle 時間: 2025-3-26 21:23 作者: Discrete 時間: 2025-3-27 04:06
DNA Delivery to Cells in Culture Using Cationic Liposomesmonstrated to react spontaneously with anionic DNA to form lipid-DNA complexes, or “l(fā)ipoplexes” (.). These lipoplexes demonstrated effective binding to cells in vitro and facilitation of intracellular delivery of a transgene. Felgner provides a detailed history of the progress leading up to and including lipofection (.).作者: OATH 時間: 2025-3-27 07:56 作者: 忍受 時間: 2025-3-27 11:44
Delivery of DNA to Cells in Culture Using Particle Bombardmentmammalian cells in vitro, in this chapter I describe protocols using the PDS-1000/He. In experiments comparing gene expression in cells bombarded using both instruments, expression is routinely two- to three-fold higher in cells bombarded using the PDS-1000/He (unpublished results).作者: Receive 時間: 2025-3-27 15:57
Delivery of DNA to Skin by Particle Bombardmentd the Helios gene-gun system commercially available at Bio-Rad Laboratories. In this chapter, the Bio-Rad gene-gun system is used as a model to describe the delivery of DNA to skin by particle bombardment and the parameters affecting such gene transfer.作者: 不能逃避 時間: 2025-3-27 19:17 作者: Homocystinuria 時間: 2025-3-28 01:53
William C. HeiserIncludes supplementary material: 作者: 花爭吵 時間: 2025-3-28 03:56 作者: Commemorate 時間: 2025-3-28 09:20 作者: sphincter 時間: 2025-3-28 11:21 作者: acrobat 時間: 2025-3-28 15:49
Carey DeMichelis,Michel Ferrarionic peptides will play an increasingly important role with in vitro and in vivo gene delivery systems. Cationic peptides usually bind through ionic interactions to the negatively charged phosphate backbone of DNA, although additional noncovalent bonds may stabilize the interaction between the polym作者: 憤慨一下 時間: 2025-3-28 19:04
https://doi.org/10.1007/978-1-4757-9673-5rst described by Nielsen et al. in 1991 (.), possess a number of useful properties including rapid and high-affinity binding to DNA, RNA, and PNA, resistance to degradation by nucleases and proteases and poor affinity for proteins that normally bind nucleic acids. For these reasons and others, PNA h作者: Etching 時間: 2025-3-29 01:07
Assessment and measurement of pain,of DNA in vitro and in vivo (.-.). These nanoscopic polymers, characterized by regular dendritic branching, radial symmetry, and uniform size ranging from 4–11 nm, have excellent water solubility and biocompatibility in a broad range of concentrations. Nonmodified dendrimers have a high density of p作者: 簡潔 時間: 2025-3-29 03:20 作者: Maximizer 時間: 2025-3-29 10:45
M. Claire Greene,Jeremy C. Kanerent gene-transfer vectors are being evaluated for delivery of a variety of therapeutic genes to the airways. These include recombinant viral (adenovirus, adeno-associated virus, retrovirus) and synthetic, self-assembling (cationic lipids, polymers) vectors. Although much of the early focus of these作者: 江湖騙子 時間: 2025-3-29 13:29
Permanent Versus Temporary ENS,es the lung play a critical role in maintaining the O./CO. concentration in blood, but it also has the largest capillary bed and an extensive cell surface area to allow DNA to bind to and enter the endothelial cells. An additional advantage of the lung endothelium for DNA delivery is that the entire作者: 排出 時間: 2025-3-29 18:23 作者: 慎重 時間: 2025-3-29 21:33 作者: 瘙癢 時間: 2025-3-30 01:39 作者: ELUDE 時間: 2025-3-30 05:08 作者: capillaries 時間: 2025-3-30 11:14
Denis R. Miller M.D.,Enid A. Haupt M.D.er have the advantage of transporting the genetic material directly into the cytoplasm or nucleus of the cell, bypassing the need for specific membrane requirements for transport into the cell. Several different instrument designs have been described for biolistic delivery of genes (.-.), however, o作者: MAPLE 時間: 2025-3-30 15:08 作者: ACME 時間: 2025-3-30 17:14 作者: exostosis 時間: 2025-3-30 23:06
