作者: 荒唐 時(shí)間: 2025-3-21 23:08
Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophilaspecific integrase mediated repeated targeting (SIRT) method, which combines homologous recombination, site-specific integration, and bacterial recombineering to conduct targeted modifications of individual loci. Here we describe the recombineering designs and procedures for the introduction of epit作者: Cholagogue 時(shí)間: 2025-3-22 01:24 作者: Limpid 時(shí)間: 2025-3-22 06:17
Gene Targeting of Human Pluripotent Stem Cells by Homologous Recombinationof human development, disease pathogenesis, and the generation of screening systems to identify novel therapeutic agents. Autologous cell therapies based on patient-derived induced pluripotent stem cells also hold great promise for the treatment and correction of many inherited and acquired diseases作者: Pericarditis 時(shí)間: 2025-3-22 12:17
Methods for the Assessment of ssODN-Mediated Gene Correction Frequencies in Muscle Cellsication for the treatment of many disorders. Among those, Duchenne muscular dystrophy (DMD) represents an ideal candidate for gene editing primarily due to the large size of dystrophin, the gene responsible for the disease, which limits the use of gene replacement approaches. Critical in the evaluat作者: 跟隨 時(shí)間: 2025-3-22 16:09 作者: 跟隨 時(shí)間: 2025-3-22 17:57
Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotiing a specific target sequence in vivo, driving that site into an exploitable repair pathway. Information is transferred to the site via participation in the pathway by the second component, a donor nucleic acid, resulting in a permanent change in the target sequence. We have developed biologically 作者: Gnrh670 時(shí)間: 2025-3-23 00:45
Triplex-Mediated Genome Targeting and Editingnous recombination machinery. The former includes a repertoire of sequence-specific binding molecules for targeted induction and appropriation of this machinery, such as by triplex-forming oligonucleotides (TFOs) or triplex-forming peptide nucleic acids (PNAs) and recombinagenic donor DNA, respectiv作者: 情愛(ài) 時(shí)間: 2025-3-23 02:52
Targeting , Transposon Integrations in the Human Genometing such systems to achieve site-directed integration as compared to un-targeted integration which occurs with native or unmodified transposon systems. The naturally active . transposon system is derived from insects but has been shown to be very efficient in gene-modifying human cells. Recent effo作者: 食草 時(shí)間: 2025-3-23 07:17 作者: 托運(yùn) 時(shí)間: 2025-3-23 13:38 作者: 誘惑 時(shí)間: 2025-3-23 14:01
Designing and Testing the Activities of TAL Effector Nucleasestime-consuming experimental screening for active TALENs, a scoring system can help select optimal target sites. Here we describe a procedure to design active TALENs using a scoring system named Scoring Algorithm for Predicted TALEN Activity (SAPTA) and a method to test the activity of individual and作者: 前面 時(shí)間: 2025-3-23 21:41 作者: 土產(chǎn) 時(shí)間: 2025-3-24 00:01
Design and Analysis of Site-Specific Single-Strand Nicking Endonucleases for Gene Correctiond break-producing enzymes, and nickases have been engineered from both homing endonuclease and .I-based scaffolds. We describe the strategies used to engineer these site-specific nickases as well as the in vitro methods used to confirm their activity and specificity. Additionally, we describe the Tr作者: AVOW 時(shí)間: 2025-3-24 03:25 作者: 不可知論 時(shí)間: 2025-3-24 09:58
Book 2014enetic variants. .Gene Correction: Methods and Protocols .provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gen作者: aerial 時(shí)間: 2025-3-24 13:44 作者: 蝕刻術(shù) 時(shí)間: 2025-3-24 18:39
https://doi.org/10.1007/978-3-0348-7985-9get location. Cells transformed with the substrate undergo homologous recombination between the genomic DNA and the recombineering substrate. The recombinants are found by selection for traits carried by the recombineering substrate, usually antibiotic resistance.作者: archetype 時(shí)間: 2025-3-24 23:02
https://doi.org/10.1007/978-3-0348-9381-7ineering to conduct targeted modifications of individual loci. Here we describe the recombineering designs and procedures for the introduction of epitope tags, in-frame deletion mutations, and point mutations into plasmids that can later be used for SIRT.作者: Discrete 時(shí)間: 2025-3-25 00:44
https://doi.org/10.1007/978-1-4684-1233-8 used to isolate derivatives of the I-SceI homing endonuclease from combinatorial libraries that display altered DNA recognition specificities. The construction of plasmid expression libraries, the development of reporter strains, and the utilization of these components in the bacterial one-hybrid system are detailed.作者: Cumbersome 時(shí)間: 2025-3-25 05:49
https://doi.org/10.1007/978-3-642-76453-0affic Light Reporter system, which uses a flow cytometric assay to simultaneously detect both gene repair and mutagenic nonhomologous end-joining outcomes at a single targeted site in mammalian cells. With these methods, novel nickases can be designed and tested for use in gene correction with novel target sites.作者: 利用 時(shí)間: 2025-3-25 09:47
Design and Analysis of Site-Specific Single-Strand Nicking Endonucleases for Gene Correctionaffic Light Reporter system, which uses a flow cytometric assay to simultaneously detect both gene repair and mutagenic nonhomologous end-joining outcomes at a single targeted site in mammalian cells. With these methods, novel nickases can be designed and tested for use in gene correction with novel target sites.作者: RODE 時(shí)間: 2025-3-25 12:36
https://doi.org/10.1007/978-3-0348-9389-3ns increasingly include data utilizing DT40 cells. In this chapter, we describe the current standard methods of multiple genetic manipulation in DT40 cells. We place a particular emphasis on describing the basic concepts and theoretical background of the genetic manipulation of DT40 cells for researchers who are new to such techniques.作者: badinage 時(shí)間: 2025-3-25 18:29
https://doi.org/10.1007/978-1-349-06540-0ome, which can be achieved by targeting genes by homologous recombination. This chapter will describe a protocol for gene modification of pluripotent stem cells by homologous recombination and several methods for the screening and identification of successfully modified clones.作者: Oafishness 時(shí)間: 2025-3-25 21:49
Chemotherapy Protocols and Infusion Sequenceman genome. In this chapter, we describe methodology for engineering and characterizing chimeric . transposase enzymes, including experimental approaches for evaluating activity and targeting specificity in the human genome.作者: 陶瓷 時(shí)間: 2025-3-26 02:48
Multiple Genetic Manipulations of DT40 Cell Linens increasingly include data utilizing DT40 cells. In this chapter, we describe the current standard methods of multiple genetic manipulation in DT40 cells. We place a particular emphasis on describing the basic concepts and theoretical background of the genetic manipulation of DT40 cells for researchers who are new to such techniques.作者: 展覽 時(shí)間: 2025-3-26 04:43 作者: MAG 時(shí)間: 2025-3-26 09:04 作者: 厭食癥 時(shí)間: 2025-3-26 15:21
RecTE,-Mediated Recombineering in ,get location. Cells transformed with the substrate undergo homologous recombination between the genomic DNA and the recombineering substrate. The recombinants are found by selection for traits carried by the recombineering substrate, usually antibiotic resistance.作者: 平淡而無(wú)味 時(shí)間: 2025-3-26 20:38
Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophilaineering to conduct targeted modifications of individual loci. Here we describe the recombineering designs and procedures for the introduction of epitope tags, in-frame deletion mutations, and point mutations into plasmids that can later be used for SIRT.作者: 按等級(jí) 時(shí)間: 2025-3-26 23:59 作者: FAZE 時(shí)間: 2025-3-27 02:28 作者: enlist 時(shí)間: 2025-3-27 06:46
Reflections on Research in Chemotherapy,for the treatment of DMD. Clinical approaches to muscle disorders using ssODNs will heavily rely on the optimization of the technology and will have to take into consideration the safety, efficacy and cost of the procedure in vision of systemic delivery of the therapeutic treatment.作者: Wordlist 時(shí)間: 2025-3-27 13:07 作者: 的’ 時(shí)間: 2025-3-27 14:18
Pharmacokinetics of Anticancer Drugs,active psoralen-linked TFOs, their co-introduction into mammalian cells with donor nucleic acids, and the identification of cells with sequence conversion of the target site. We have emphasized details in the synthesis and purification of the oligonucleotides that are essential for preparation of re作者: Ligament 時(shí)間: 2025-3-27 18:29 作者: 停止償付 時(shí)間: 2025-3-28 00:32
Javaughn Corey R. Gray,Jean Hoffman-Censitsial double-strand breaks. By using electroporation for gene targeting, target cells should be expanded to 10.–10. cells. In contrast, as an advantage of virus-mediated method, DNA delivery efficiency is high even in a smaller number of cells, resulting in minimizing the number of passages/cell divis作者: 文藝 時(shí)間: 2025-3-28 04:50 作者: 潔凈 時(shí)間: 2025-3-28 08:07
Safety in the Soviet Nuclear Power Industry,hrough nonhomologous end joining (NHEJ) or through homologous recombination (HR) in the presence of an appropriate donor sequence. This RNA-guided editing tool offers greater ease of customization and synthesis in comparison to existing sequence-specific endonucleases and promises to become a highly作者: 帶來(lái)墨水 時(shí)間: 2025-3-28 12:12
Methods for the Assessment of ssODN-Mediated Gene Correction Frequencies in Muscle Cellsfor the treatment of DMD. Clinical approaches to muscle disorders using ssODNs will heavily rely on the optimization of the technology and will have to take into consideration the safety, efficacy and cost of the procedure in vision of systemic delivery of the therapeutic treatment.作者: CRAB 時(shí)間: 2025-3-28 15:50
Small Fragment Homologous Replacement (SFHR): Sequence-Specific Modification of Genomic DNA in Eukar atrophy, cystic fibrosis, severe combined immune deficiency). Several parameters can be modified to optimize the gene modification efficiency, as described in our recent study..In this chapter we describe the main guidelines that should be followed in SFHR application, in order to increase techniqu作者: Infelicity 時(shí)間: 2025-3-28 22:15
Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotiactive psoralen-linked TFOs, their co-introduction into mammalian cells with donor nucleic acids, and the identification of cells with sequence conversion of the target site. We have emphasized details in the synthesis and purification of the oligonucleotides that are essential for preparation of re作者: adjacent 時(shí)間: 2025-3-29 00:53 作者: sclera 時(shí)間: 2025-3-29 06:56 作者: 金絲雀 時(shí)間: 2025-3-29 09:37
Lentiviral Vectors Encoding Zinc-Finger Nucleases Specific for the Model Target Locus ,rapid and simple restriction enzyme site polymorphism assay to detected DSB formation at the . target sequence. Owing in part to the small molecule-based clone selection schemes conferred by . allelic knockouts, this X-linked gene has become a “classical” target model locus in mammalian cells. The r作者: BET 時(shí)間: 2025-3-29 15:12
CRISPR-Cas-Mediated Targeted Genome Editing in Human Cellshrough nonhomologous end joining (NHEJ) or through homologous recombination (HR) in the presence of an appropriate donor sequence. This RNA-guided editing tool offers greater ease of customization and synthesis in comparison to existing sequence-specific endonucleases and promises to become a highly作者: 孵卵器 時(shí)間: 2025-3-29 19:10 作者: 招惹 時(shí)間: 2025-3-29 19:59
Enhanced Gene Targeting of Adult and Pluripotent Stem Cells Using Evolved Adeno-associated Virus作者: Little 時(shí)間: 2025-3-30 03:34
https://doi.org/10.1007/978-1-62703-761-7gene correction; gene repair; gene targeting; human genome; human pluripotent stem cells作者: Explosive 時(shí)間: 2025-3-30 06:26 作者: Perceive 時(shí)間: 2025-3-30 12:10
https://doi.org/10.1007/978-3-0348-9381-7specific integrase mediated repeated targeting (SIRT) method, which combines homologous recombination, site-specific integration, and bacterial recombineering to conduct targeted modifications of individual loci. Here we describe the recombineering designs and procedures for the introduction of epit作者: 成績(jī)上升 時(shí)間: 2025-3-30 14:22 作者: 混合物 時(shí)間: 2025-3-30 18:39
https://doi.org/10.1007/978-1-349-06540-0of human development, disease pathogenesis, and the generation of screening systems to identify novel therapeutic agents. Autologous cell therapies based on patient-derived induced pluripotent stem cells also hold great promise for the treatment and correction of many inherited and acquired diseases作者: LAY 時(shí)間: 2025-3-30 21:34
Reflections on Research in Chemotherapy,ication for the treatment of many disorders. Among those, Duchenne muscular dystrophy (DMD) represents an ideal candidate for gene editing primarily due to the large size of dystrophin, the gene responsible for the disease, which limits the use of gene replacement approaches. Critical in the evaluat作者: Foam-Cells 時(shí)間: 2025-3-31 04:52
T. A. Khwaja,T. C. Hall,K. M. A. Sheikhctive approach for gene therapy. Small DNA fragments (SDFs) were used in SFHR to modify endogenous genomic DNA in both human and murine cells. The advantage of this gene targeting approach is to maintain the physiologic expression pattern of targeted genes without altering the regulatory sequences (作者: 職業(yè)拳擊手 時(shí)間: 2025-3-31 06:54
Pharmacokinetics of Anticancer Drugs,ing a specific target sequence in vivo, driving that site into an exploitable repair pathway. Information is transferred to the site via participation in the pathway by the second component, a donor nucleic acid, resulting in a permanent change in the target sequence. We have developed biologically 作者: 襲擊 時(shí)間: 2025-3-31 11:42
Bruce A. Chabner,David G. Johnsnous recombination machinery. The former includes a repertoire of sequence-specific binding molecules for targeted induction and appropriation of this machinery, such as by triplex-forming oligonucleotides (TFOs) or triplex-forming peptide nucleic acids (PNAs) and recombinagenic donor DNA, respectiv作者: 過(guò)份 時(shí)間: 2025-3-31 14:00 作者: 協(xié)議 時(shí)間: 2025-3-31 21:20
