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標題: Titlebook: Gene Cloning; The Mechanics of DNA David M. Glover Book 1984 D. M. Glover 1984 DNA.bacteria.biology.cells.cloning.gene.genes.molecule.mutag [打印本頁]

作者: Sediment    時間: 2025-3-21 19:19
書目名稱Gene Cloning影響因子(影響力)




書目名稱Gene Cloning影響因子(影響力)學科排名




書目名稱Gene Cloning網(wǎng)絡公開度




書目名稱Gene Cloning網(wǎng)絡公開度學科排名




書目名稱Gene Cloning被引頻次




書目名稱Gene Cloning被引頻次學科排名




書目名稱Gene Cloning年度引用




書目名稱Gene Cloning年度引用學科排名




書目名稱Gene Cloning讀者反饋




書目名稱Gene Cloning讀者反饋學科排名





作者: exhibit    時間: 2025-3-21 21:25
Overview: 978-0-412-26600-3978-1-4899-3246-4
作者: 向下五度才偏    時間: 2025-3-22 02:44
Transport and Transformation Processesial plasmids. Of the bacteriophage themselves, the . phage . is perhaps the best characterized. The interaction of phage . with the host cell is a particularly fruitful area of study, as here are a set of genes which can either direct cell lysis or become stably associated with the host chromosome i
作者: STRIA    時間: 2025-3-22 05:45

作者: TEN    時間: 2025-3-22 11:52
Stefano Moro,Magdalena Bacilieri genetics of the phage [1, 2] has greatly assisted its development as a cloning vehicle. It has about 50 genes in its 49 kb genome and of these only about half are essential. The nonessential genes can be replaced with foreign DNA and the recombinant genome DNA can then be propagated in .. This chap
作者: 缺乏    時間: 2025-3-22 15:24
https://doi.org/10.1007/1-4020-5001-1e bacterial strain to another through a conjugative process. The archetypal example of a conjugative plasmid is the F factor of .. The transfer of DNA from a male strain of . carrying the F genome to a female strain which lacks the F genome, requires the products of about 20 transfer (.) genes which
作者: 缺乏    時間: 2025-3-22 19:29
Tracy Fischer-Smith,Jay Rappaportal and translational machinery of the host cell. The bacterium . is a natural choice for such studies because of our detailed knowledge of the molecular biology of its gene expression. A vast amount of effort has now been put into the development of plasmid vectors to maximize the expression of fore
作者: Blemish    時間: 2025-3-23 00:09

作者: 健談    時間: 2025-3-23 04:19

作者: 越自我    時間: 2025-3-23 08:17

作者: Liberate    時間: 2025-3-23 10:41
Stefano Moro,Magdalena Bacilieribout half are essential. The nonessential genes can be replaced with foreign DNA and the recombinant genome DNA can then be propagated in .. This chapter will trace the development of a versatile series of vectors for different cloning problems.
作者: 發(fā)怨言    時間: 2025-3-23 15:04

作者: 換話題    時間: 2025-3-23 21:59

作者: 音樂等    時間: 2025-3-24 01:44
Gene cloning in fungi and plants, cell wall. The spheroplasts, so produced, can be maintained in isotonic solutions of sorbitol and DNA uptake can be promoted by the addition of polyethylene glycol. The cell wall regenerates if the treated spheroplasts are plated in 3% agar.
作者: Parameter    時間: 2025-3-24 03:30
Tracy Fischer-Smith,Jay Rappaportign DNA in . It is outside the scope of this chapter to fully document these efforts, which have been reviewed elsewhere [1], Instead we will look at essential features of such heterologous expression systems.
作者: seroma    時間: 2025-3-24 08:51
Expression of cloned DNAs in , plasmids,ign DNA in . It is outside the scope of this chapter to fully document these efforts, which have been reviewed elsewhere [1], Instead we will look at essential features of such heterologous expression systems.
作者: PATHY    時間: 2025-3-24 13:20
The principles of cloning DNA,ial plasmids. Of the bacteriophage themselves, the . phage . is perhaps the best characterized. The interaction of phage . with the host cell is a particularly fruitful area of study, as here are a set of genes which can either direct cell lysis or become stably associated with the host chromosome i
作者: TATE    時間: 2025-3-24 18:49

作者: 喚起    時間: 2025-3-24 23:04

作者: 嬉耍    時間: 2025-3-24 23:55

作者: 使乳化    時間: 2025-3-25 07:15
Expression of cloned DNAs in , plasmids,al and translational machinery of the host cell. The bacterium . is a natural choice for such studies because of our detailed knowledge of the molecular biology of its gene expression. A vast amount of effort has now been put into the development of plasmid vectors to maximize the expression of fore
作者: 自戀    時間: 2025-3-25 10:26
The physical characterization of cloned DNA segments and their counterparts within chromosomes,ouse, for example, contains approximately 3 × 10. kb of DNA. The purification and characterization of a gene such as that encoding .-globin was impossible before the advent of such techniques. The techniques for gene cloning not only enable a gene to be purified but also permit the amplification of
作者: 揭穿真相    時間: 2025-3-25 13:32

作者: 一夫一妻制    時間: 2025-3-25 18:52

作者: Foolproof    時間: 2025-3-25 21:37
Transport and Transformation Processesc acid hybridization or enabling the production of large amounts of particular gene products. Research on the bacterial plasmids has had a similar history. The discovery and rationalization of the mechanism whereby F factors promote bacterial conjugation was central to the development of . genetics.
作者: Dysplasia    時間: 2025-3-26 00:57
Virtual screening by molecular dockingM and .R. A polypeptide which governs the specificity of the system is determined by .S. The gene product of .M is the modification enzyme which also interacts with the product of the .R gene, the restriction endonuclease, in the cleavage process. In the above examples the phage grown on strain K wo
作者: 含鐵    時間: 2025-3-26 05:54
https://doi.org/10.1007/1-4020-5001-1as a result of laboratory experimentation. The fear was that a potentially dangerous gene could be accidentally transferred to a variety of bacteria if cloned upon a vector capable of conjugation. The conjugative plasmids are capable, not only of their own transmission from one cell to another, but
作者: irreducible    時間: 2025-3-26 10:03
Rupert P. M. Negus,Frances R. Balkwillir structural genes, and why gene transcripts in the nuclei of most higher organisms are very much larger and more heterogeneous than the functional mRNAs in the cell cytoplasm. Our picture of gene control in eukaryotes is, however, still very incomplete in spite of the information explosion generat
作者: 費解    時間: 2025-3-26 14:40

作者: 迅速成長    時間: 2025-3-26 20:47

作者: dermatomyositis    時間: 2025-3-27 00:58
Recombination and mutagenesis of DNA ,,M and .R. A polypeptide which governs the specificity of the system is determined by .S. The gene product of .M is the modification enzyme which also interacts with the product of the .R gene, the restriction endonuclease, in the cleavage process. In the above examples the phage grown on strain K wo
作者: jabber    時間: 2025-3-27 03:26

作者: 機械    時間: 2025-3-27 07:30

作者: Hormones    時間: 2025-3-27 11:55
Expression of cloned genes in animal cells,growth in HAT medium. The observation that carrier DNA greatly enhanced the transformation frequency led to successful attempts to transfer single copy eukaryotic genes using total genomic DNA [3]. Wigler and colleagues then demonstrated that unlinked, non-selectable genes could be cotransformed whe
作者: agglomerate    時間: 2025-3-27 16:16

作者: Fallibility    時間: 2025-3-27 18:17
Conference proceedings 2021first ENUMATH was held in Paris in 1995, with successive installments at various sites across Europe, including Heidelberg (1997), Jyvaskyla (1999), lschia Porto (2001), Prague (2003), Santiago de Compostela (2005), Graz (2007), Uppsala (2009), Leicester (2011), Lausanne (2013), Ankara (2015) and Bergen (2017)..
作者: Nonporous    時間: 2025-3-27 21:56
Pierre F. Monsan,Francois Ouarnéood pressure, because of the great reduction in total peripheral resistance which immediately ensues. A point to emphasize, however, is that this fall of blood pressure, which can be quite prolonged, must be due mainly to vasodilation in the splanchnic bed, because this, more than any other, is the
作者: deriver    時間: 2025-3-28 03:38
Responses of Whole Plants to Air Pollutantsype” pollution). Photochemical smog is produced in the atmosphere by complex photochemical reactions involving nitrogen oxides and hydrocarbons from sources such as auto exhaust gases and electric power plants. A similarly serious air pollution problem has now emerged in large urban cities in develo
作者: 朦朧    時間: 2025-3-28 06:49
R. Kolvenbach,H. Kniemeyer,W. Sandmann,H. Reintgesse controversies are best resolved when the bilateral relations between the states concerned are otherwise good, when it is in their mutual interest to reach agreement, or when the asymmetry between the states involved is clearly defined and the more powerful state decides to end the dispute.
作者: STYX    時間: 2025-3-28 11:02
https://doi.org/10.1007/978-3-322-84122-3Herrschaft; Kapital; Krieg; Kriegsende; Macht; Politik; Verwaltung; Weltkrieg; Westen; deutsche Besatzung
作者: 簡略    時間: 2025-3-28 16:04
0939-6055 ntial to stay abreast of the most recent ideas and developments and to become familiar with certain topics in the field of sedimentary geology. This series deals with subjects that are in the forefront of both scientific and economic interests. The treatment of a subject in an individual volume, the
作者: 漂亮    時間: 2025-3-28 21:14





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