標題: Titlebook: Gap Junction Protocols; Mathieu Vinken,Scott R. Johnstone Book 2016 Springer Science+Business Media, LLC, part of Springer Nature 2016 Con [打印本頁] 作者: 非決定性 時間: 2025-3-21 18:55
書目名稱Gap Junction Protocols影響因子(影響力)
書目名稱Gap Junction Protocols影響因子(影響力)學科排名
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書目名稱Gap Junction Protocols網(wǎng)絡公開度學科排名
書目名稱Gap Junction Protocols被引頻次
書目名稱Gap Junction Protocols被引頻次學科排名
書目名稱Gap Junction Protocols年度引用
書目名稱Gap Junction Protocols年度引用學科排名
書目名稱Gap Junction Protocols讀者反饋
書目名稱Gap Junction Protocols讀者反饋學科排名
作者: Highbrow 時間: 2025-3-21 23:20
Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photoactivation and Fluorescence Im by carrying out multiple photo-activations in a coupled cell pair, LAMP assay can track changes in cell coupling strength between coupled cells, hence providing a powerful method for investigating the regulation of junctional coupling by cellular biochemical changes.作者: Visual-Field 時間: 2025-3-22 01:24
Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junave propagation was assayed by imaging fluorescent calcium in bovine corneal endothelial cells loaded with a fluorescent calcium dye using a confocal microscope. Spatial changes in intercellular calcium concentration following mechanical stimulation were measured in the mechanical stimulated cell an作者: 喧鬧 時間: 2025-3-22 05:55 作者: 賄賂 時間: 2025-3-22 09:18 作者: 褪色 時間: 2025-3-22 16:05
Florent de Dinechin,Martin Kumm by carrying out multiple photo-activations in a coupled cell pair, LAMP assay can track changes in cell coupling strength between coupled cells, hence providing a powerful method for investigating the regulation of junctional coupling by cellular biochemical changes.作者: 褪色 時間: 2025-3-22 18:28 作者: Nostalgia 時間: 2025-3-22 23:58 作者: 過濾 時間: 2025-3-23 03:51
DNA Methylation Analysis of Human Tissue-Specific Connexin Genes,hylation of connexin genes, play important roles in regulating gene expression. Over the past decade, several methods have been applied to characterize DNA methylation-specific loci of connexin genes. This chapter describes analysis of selective connexin32 and connexin43 gene DNA methylation in huma作者: 輕浮女 時間: 2025-3-23 07:55
Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoressearch field. The present chapter describes the setup of an immunoblot procedure, including protein extraction and quantification from biological samples, gel electrophoresis, protein transfer, and immunoblotting, which is optimized for analysis of connexins in liver tissue. In essence, proteins are作者: Harness 時間: 2025-3-23 09:48 作者: 先鋒派 時間: 2025-3-23 16:48
Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Ceferent connexin isoforms. Thus, specific connexin gene knockdown using RNAi-mediated technologies is a technique that allows investigators to efficiently monitor silencing effects of single or multiple connexin gene products. The present chapter describes the transient knockdown of connexins in vitr作者: HEDGE 時間: 2025-3-23 18:25
Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins fo mechanisms, the use of cell culture systems is essential to discriminate between the effects of different connexin isoforms expressed in individual cells or tissues of the developing conceptus or in maternal reproductive tissues. The generation and analysis of gene-deficient trophoblast stem cell l作者: Insubordinate 時間: 2025-3-23 23:05 作者: entitle 時間: 2025-3-24 03:56 作者: CLAIM 時間: 2025-3-24 08:04 作者: 他姓手中拿著 時間: 2025-3-24 10:48 作者: 裂縫 時間: 2025-3-24 15:50
Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Couplingor the maintenance of tissue homeostasis. Gap junction channels are protein channels that are situated between neighboring cells and that provide a direct, yet selective route for the passage of small hydrophilic biomolecules and ions. Here, an electroporation method is described to load a localized作者: Curmudgeon 時間: 2025-3-24 21:55
Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro,gh initially developed to investigate intracellular mobility, FRAP can be also used to measure intercellular dynamics. This chapter describes how to perform FRAP experiment to study gap junctional communication in living cells. The procedures described here can be carried out with a laser-scanning c作者: 好忠告人 時間: 2025-3-25 02:46 作者: 要素 時間: 2025-3-25 04:39 作者: Adulterate 時間: 2025-3-25 08:45
Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junto communicate between cells and organs, including the brain, lung, liver, lens, retina, and heart. Gap junctions enable a direct route for ions like calcium and potassium, and low molecular weight compounds, such as inositol 1,4,5-trisphosphate, cyclic adenosine monophosphate, and various kinds of 作者: 拋棄的貨物 時間: 2025-3-25 15:13
Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gaplls with resolution to the single channel level. The dual patch clamp recording technique requires specialized equipment and the acquired skill to reliably establish gigaohm seals and the whole cell recording configuration with high efficiency. This chapter describes the equipment needed and methods作者: intertwine 時間: 2025-3-25 15:50
https://doi.org/10.1007/978-1-4939-3664-9Connexin expression; Cell signaling; Connexons; Gap junctional intercellular communication; GJIC regulat作者: fibula 時間: 2025-3-25 23:43 作者: Affiliation 時間: 2025-3-26 03:41
https://doi.org/10.1007/978-1-4757-4523-8hylation of connexin genes, play important roles in regulating gene expression. Over the past decade, several methods have been applied to characterize DNA methylation-specific loci of connexin genes. This chapter describes analysis of selective connexin32 and connexin43 gene DNA methylation in huma作者: 墊子 時間: 2025-3-26 05:12 作者: 掙扎 時間: 2025-3-26 11:55
Optimization Algorithms and Applications,the connexin research field, IHC is applied to identify the subcellular location of connexin proteins, as this can be directly linked to their functionality. The present chapter describes a protocol for fluorescent IHC to detect connexin proteins in tissues slices and cells, with slight modification作者: 擦掉 時間: 2025-3-26 12:56
https://doi.org/10.1007/978-94-6209-332-4ferent connexin isoforms. Thus, specific connexin gene knockdown using RNAi-mediated technologies is a technique that allows investigators to efficiently monitor silencing effects of single or multiple connexin gene products. The present chapter describes the transient knockdown of connexins in vitr作者: Adenocarcinoma 時間: 2025-3-26 20:13 作者: incisive 時間: 2025-3-27 00:11
https://doi.org/10.1007/978-1-4899-8041-0minal domains). The c-terminal domains vary in length and amino acid composition from the shortest on Cx26 to the longest on Cx43. With the exception of Cx26, the c-terminal domains contain multiple sites for posttranslational modification (PTM) including serines (S), threonines (T), and tyrosines (作者: Muffle 時間: 2025-3-27 01:48
Springer Desktop Editions in Chemistry interactions of integral membrane proteins is a valuable tool for determining channel function. However, several technical challenges exist. Subcellular fractionation of the bait protein matrix is usually required to identify less abundant proteins in complex homogenates. Sufficient solvation of th作者: xanthelasma 時間: 2025-3-27 07:09
Emilie Balátová-Tulá?ková,Werner Krausecation (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a 作者: Tempor 時間: 2025-3-27 10:22
https://doi.org/10.1007/978-3-030-61408-9 Assessing these channels is therefore fundamental to understanding disease pathophysiology, developing therapies for a multitude of acquired and genetic conditions, and providing novel approaches to drug delivery and cellular communication. Microinjection is a robust, albeit difficult, technique, w作者: Occupation 時間: 2025-3-27 15:30 作者: 出價 時間: 2025-3-27 21:51 作者: motor-unit 時間: 2025-3-28 01:52 作者: Platelet 時間: 2025-3-28 04:14 作者: BATE 時間: 2025-3-28 07:04
Shafique Matin,Sisir Kumar Dash,Tune Ushato communicate between cells and organs, including the brain, lung, liver, lens, retina, and heart. Gap junctions enable a direct route for ions like calcium and potassium, and low molecular weight compounds, such as inositol 1,4,5-trisphosphate, cyclic adenosine monophosphate, and various kinds of 作者: 泰然自若 時間: 2025-3-28 12:44
L. Vamling,M. H?gberg,T. Berntssonlls with resolution to the single channel level. The dual patch clamp recording technique requires specialized equipment and the acquired skill to reliably establish gigaohm seals and the whole cell recording configuration with high efficiency. This chapter describes the equipment needed and methods作者: Conscientious 時間: 2025-3-28 14:49 作者: Reservation 時間: 2025-3-28 22:15 作者: 正常 時間: 2025-3-29 01:58
Gap Junction Protocols978-1-4939-3664-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 銀版照相 時間: 2025-3-29 03:14 作者: 音的強弱 時間: 2025-3-29 07:42 作者: ACME 時間: 2025-3-29 14:25 作者: 彈藥 時間: 2025-3-29 19:39 作者: 該得 時間: 2025-3-29 22:22
Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerasewell-established reverse transcription quantitative real-time polymerase chain reaction procedure optimized for analysis of hepatic connexins. The method includes RNA extraction and subsequent quantification, generation of complementary DNA, quantitative real-time polymerase chain reaction, and data analysis.作者: 不足的東西 時間: 2025-3-30 03:38
DNA Methylation Analysis of Human Tissue-Specific Connexin Genes,e DNA methylation-specific loci of connexin genes. This chapter describes analysis of selective connexin32 and connexin43 gene DNA methylation in human gastric tissues using methylation-specific PCR, bisulfite-specific PCR sequencing as well as MassArray techniques.作者: Etching 時間: 2025-3-30 05:22 作者: dendrites 時間: 2025-3-30 11:27 作者: 和平主義者 時間: 2025-3-30 14:59
Springer Desktop Editions in Chemistryfor translation to other integral membrane proteins, tissues, or cell culture models. These procedures are valuable tools for the enrichment of gap junction membrane compartments and for the identification of gap junction signaling interactomes.作者: 排斥 時間: 2025-3-30 16:56
https://doi.org/10.1007/978-3-030-61408-9of new, automation technologies may improve further. This text describes the basic technique of microinjection and briefly discusses modern automation advances that can improve the success rates of this technique.作者: Feigned 時間: 2025-3-30 21:19 作者: 閃光東本 時間: 2025-3-31 04:48 作者: 都相信我的話 時間: 2025-3-31 07:18 作者: 粘 時間: 2025-3-31 10:44 作者: Felicitous 時間: 2025-3-31 14:45
