標(biāo)題: Titlebook: Galectins; Methods and Protocol Sean R. Stowell,Connie M. Arthur,Richard D. Cummin Book 2022Latest edition Springer Science+Business Media, [打印本頁] 作者: 嚴(yán)峻 時間: 2025-3-21 16:18
書目名稱Galectins影響因子(影響力)
書目名稱Galectins影響因子(影響力)學(xué)科排名
書目名稱Galectins網(wǎng)絡(luò)公開度
書目名稱Galectins網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Galectins被引頻次
書目名稱Galectins被引頻次學(xué)科排名
書目名稱Galectins年度引用
書目名稱Galectins年度引用學(xué)科排名
書目名稱Galectins讀者反饋
書目名稱Galectins讀者反饋學(xué)科排名
作者: surmount 時間: 2025-3-21 23:33 作者: 施加 時間: 2025-3-22 03:34 作者: 形狀 時間: 2025-3-22 07:55 作者: phytochemicals 時間: 2025-3-22 10:04
Rapid Detection and Purification of Galectin-3 by the ,pture and ,lease (CaRe) Method, glycoproteins in those processes, it is essential to detect these proteins in biological samples and purify them to homogeneity. Conventional protein detection and purification techniques are multi-step, time-intensive, and expensive. They often require rigorous trial and error experimentations and作者: Initial 時間: 2025-3-22 15:14
,Introducing 77Se NMR Spectroscopy to Analyzing Galectin –Ligand Interaction,mer(s) of their ligands in solution. Basically, NMR spectroscopy facilitates respective experiments. Towards developing new and even better approaches for these purposes, extending the range of exploitable isotopes beyond .H, .C, and .N offers promising perspectives. Having therefore prepared seleno作者: Initial 時間: 2025-3-22 19:45
Evaluation of Galectin Binding by Surface Plasmon Resonance,sensitivity and low sample consumption in the Biacore enables the measurement of rapid kinetics and low affinities characteristics of many biological interactions. This chapter describes the affinity measurement of Galectins-1, -2 and -3 and their glycoside ligands using this approach.作者: 迷住 時間: 2025-3-22 22:31 作者: vanquish 時間: 2025-3-23 04:07 作者: 燈絲 時間: 2025-3-23 06:01
Mechanism of Mucin Recognition by Lectins : A Thermodynamic Study,stant, binding stoichiometry, heat of binding (enthalpy) and indirectly the entropy, and free energy of binding. ITC has been extensively used to study the binding of lectins to mono- and oligosaccharides, but limitedly in applications to lectin–glycoprotein interactions. Inherent experimental chall作者: Demonstrate 時間: 2025-3-23 12:04
Examination of Whole-Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis,rface glycoconjugates. These methods utilize commonly employed flow cytometry techniques and commercially available streptavidin-coated microplates to immobilize various biotinylated ligands, such as glycopeptides, oligosaccharides, and whole cells. Using this approach, fluorescently labeled GBPs, i作者: Shuttle 時間: 2025-3-23 15:36
Dissecting Context-Specific Galectin Binding Using Glycoengineered Cell Libraries,ying β-galactoside-containing glycans. To understand the diversity of functions governed by galectins, it is essential to define the binding specificity of the carbohydrate recognition domain (CRDs) of the individual galectins. The binding specificity of galectins has primarily been examined with gl作者: lesion 時間: 2025-3-23 21:19
Method for Identifying Galectin Ligands on Lymphocyte Membrane Glycoproteins,an structures, and functional studies have indicated that a family of glycan-binding proteins, galectins, can significantly modulate lymphocyte development and function by interacting with these glycans. Several galectins have a varying degree of affinity for the .acetyllactosamine (LacNAc) disaccha作者: 糾纏,纏繞 時間: 2025-3-24 01:29
,Transformation of , Galectin into αGalNAc-Specific Lectin,cted mutagenesis of the hydrophilic residues His, Asn, Arg, and Glu, involved in carbohydrate recognition, abolished the binding affinity of the derived mutants to β-galactosides, whereas only N46A caused increased affinity to GalNAcα1-3Gal-containing oligosaccharides and loss of β-galactoside-bindi作者: 商議 時間: 2025-3-24 06:17
What Happens If a Human Galectin Enters the Endoplasmic Reticulum?,rresponding engineering of galectin-specific cDNA will answer questions on the fate of a signal peptide-bearing protein variant after its entry into the endoplasmic reticulum (ER). Affinity chromatography and mass-spectrometric analysis of occupancy of potential N-glycosylation sites for the galecti作者: PACK 時間: 2025-3-24 08:39 作者: perpetual 時間: 2025-3-24 10:53
Exploring the Galectin Network by Light and Fluorescence Microscopy,atus of glycan branching or chain length (e.g., core 1 vs core 2 mucin-type O-glycans and polyLacNAc additions) as well as of sialylation/sulfation has been delineated to convey signals. They are “read” by galectins, for example regulating lattice formation on the membrane and cell growth. Owing to 作者: 面包屑 時間: 2025-3-24 15:06 作者: 怒目而視 時間: 2025-3-24 19:08
Visualization of Cytosolic Galectin Accumulation Around Damaged Vesicles and Organelles,ally exposed glycans and accumulate around endocytic vesicles or organelles damaged by various disruptive substances. Accumulated galectins engage other cytosolic proteins toward damaged vesicles, leading to cellular responses, such as autophagy. Disruptive substances include bacteria, viruses, part作者: 臨時抱佛腳 時間: 2025-3-25 02:23
https://doi.org/10.1007/978-3-662-04240-3enome duplications, over 15 known vertebrate galectins likely evolved from a single common ancestor extant in pre-chordate lineages. While galectins have divergently evolved numerous functions, some of which do not involve carbohydrate recognition, the vast majority of the galectins have retained th作者: Noisome 時間: 2025-3-25 03:28 作者: 抱狗不敢前 時間: 2025-3-25 08:55
Mehrdimensionale Verdichterbeschaufelung,, the exact physiological and biochemical functions mediated by galectins that necessitate their wide occurrence among diverse species have not yet been delineated in a precise manner. Purification of recombinant galectins in active form is a fundamental requirement to elucidate their biological fun作者: 萬花筒 時間: 2025-3-25 15:13
https://doi.org/10.1007/978-3-322-89735-0ion. While a variety of approaches can be utilized to prevent galectin oxidation, several of these require inclusion of reducing agents that not only prevent galectins from undergoing oxidative inactivation but can also interfere with normal redox potentials required for fundamental cellular process作者: FOR 時間: 2025-3-25 19:21 作者: Melatonin 時間: 2025-3-25 22:39
Aerodynamik der stumpfen K?rpermer(s) of their ligands in solution. Basically, NMR spectroscopy facilitates respective experiments. Towards developing new and even better approaches for these purposes, extending the range of exploitable isotopes beyond .H, .C, and .N offers promising perspectives. Having therefore prepared seleno作者: 爆米花 時間: 2025-3-26 01:18
Aerodynamik der stumpfen K?rpersensitivity and low sample consumption in the Biacore enables the measurement of rapid kinetics and low affinities characteristics of many biological interactions. This chapter describes the affinity measurement of Galectins-1, -2 and -3 and their glycoside ligands using this approach.作者: floaters 時間: 2025-3-26 06:26
https://doi.org/10.1007/978-3-642-53044-9ycoconjugates. Specific hydroxyl groups (4-OH, 6-OH of galactose, and 3-OH of glucose/.-acetylglucosamine) of lactose/LacNAc are essential for their binding to Gal-3. Through hemagglutination inhibition, microcalorimetry, and spectroscopy, we have shown that despite being a lectin, Gal-3 possesses t作者: 倫理學(xué) 時間: 2025-3-26 08:51
https://doi.org/10.1007/978-3-642-96046-8le cell surface glycans. While many studies demonstrate the impact of glycan modification on GBP recognition and activity, the relative contribution of subtle changes in glycan structure on GBP binding can be difficult to define. To overcome limitations in the analysis of GBP–glycan interactions, re作者: Habituate 時間: 2025-3-26 15:44 作者: 壕溝 時間: 2025-3-26 19:04 作者: calorie 時間: 2025-3-26 22:17 作者: 青石板 時間: 2025-3-27 04:28
https://doi.org/10.1007/978-1-4614-3609-6an structures, and functional studies have indicated that a family of glycan-binding proteins, galectins, can significantly modulate lymphocyte development and function by interacting with these glycans. Several galectins have a varying degree of affinity for the .acetyllactosamine (LacNAc) disaccha作者: 欄桿 時間: 2025-3-27 09:18
Air Evacuation of the Neurosurgical Patientcted mutagenesis of the hydrophilic residues His, Asn, Arg, and Glu, involved in carbohydrate recognition, abolished the binding affinity of the derived mutants to β-galactosides, whereas only N46A caused increased affinity to GalNAcα1-3Gal-containing oligosaccharides and loss of β-galactoside-bindi作者: 平靜生活 時間: 2025-3-27 13:06
Elizabeth Bridges,Melissa A. Buzbee-Stilesrresponding engineering of galectin-specific cDNA will answer questions on the fate of a signal peptide-bearing protein variant after its entry into the endoplasmic reticulum (ER). Affinity chromatography and mass-spectrometric analysis of occupancy of potential N-glycosylation sites for the galecti作者: 群島 時間: 2025-3-27 13:37
First Helicopters and Rotor Systems and are considered as not only biomarkers of human diseases but also molecular targets for treating cancer and inflammatory illnesses in many organs. In the glycobiology field, it is crucial to determine the pattern of galectin expression and location in cells and tissues. Confocal microscopy is a 作者: 征服 時間: 2025-3-27 19:03
Anomalies in the Regulatory Structure,atus of glycan branching or chain length (e.g., core 1 vs core 2 mucin-type O-glycans and polyLacNAc additions) as well as of sialylation/sulfation has been delineated to convey signals. They are “read” by galectins, for example regulating lattice formation on the membrane and cell growth. Owing to 作者: Omniscient 時間: 2025-3-27 22:30 作者: Anthrp 時間: 2025-3-28 02:34
Aeronomy of the Middle Atmosphereally exposed glycans and accumulate around endocytic vesicles or organelles damaged by various disruptive substances. Accumulated galectins engage other cytosolic proteins toward damaged vesicles, leading to cellular responses, such as autophagy. Disruptive substances include bacteria, viruses, part作者: 緯線 時間: 2025-3-28 07:17
https://doi.org/10.1007/978-1-0716-2055-7oxidative inactivation; thermodynamics; glycan microarrays; neoplastic disease; cancer cells作者: 牽連 時間: 2025-3-28 10:44 作者: 不自然 時間: 2025-3-28 17:34
Evaluation of Galectin Binding by Surface Plasmon Resonance,sensitivity and low sample consumption in the Biacore enables the measurement of rapid kinetics and low affinities characteristics of many biological interactions. This chapter describes the affinity measurement of Galectins-1, -2 and -3 and their glycoside ligands using this approach.作者: ineffectual 時間: 2025-3-28 19:11
Sean R. Stowell,Connie M. Arthur,Richard D. CumminIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: 非秘密 時間: 2025-3-28 23:40 作者: 清澈 時間: 2025-3-29 03:07
Galectins978-1-0716-2055-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: floodgate 時間: 2025-3-29 09:25
https://doi.org/10.1007/978-3-662-04240-3ncovered intracellular galectin functions mediated by carbohydrate- and non-carbohydrate-dependent interactions. In the cytoplasm, galectins can tune intracellular kinase and G-protein-coupled signaling cascades important for nutrient sensing, cell cycle progression, and transformation. Particularly作者: Infant 時間: 2025-3-29 11:25 作者: COMMA 時間: 2025-3-29 15:50
https://doi.org/10.1007/978-3-0348-5295-1ng/purifying glycoproteins. In this method, targets (lectins and glycoproteins) are captured by multivalent ligands called target capturing agents (TCAs). The captured targets are then released and separated from their TCAs to obtain purified targets. CaRe can potentially be used as a tool to discov作者: generic 時間: 2025-3-29 23:13 作者: mydriatic 時間: 2025-3-30 00:35
https://doi.org/10.1007/978-3-642-53044-9they formed reversible noncovalent cross-linked complexes with the lectin. Binding of sulfated GAGs to Gal-3 was completely inhibited when Gal-3 was preincubated with β-lactose. Cross-linking of Gal-3 by CSA, CSC, and the bovine CSPG was also reversed by β-lactose. These findings strongly suggest th作者: 改正 時間: 2025-3-30 05:18
https://doi.org/10.1007/978-3-662-05619-6bohydrate to carbohydrate epitope in these molecules leading to increased affinity. Importantly, the mechanism of binding of lectins to mucins appears similar to that for a variety of protein ligands binding to DNA. Recent results also show that high-affinity lectin–mucin cross-linking interactions 作者: MAG 時間: 2025-3-30 09:42 作者: 推崇 時間: 2025-3-30 16:27
First Helicopters and Rotor Systemsonventional microscopy and enables reconstruction of virtual tridimensional images by acquiring multiple sections from several focal planes, which makes it possible to obtain the precise spatial location of any cellular structure or molecule. Furthermore, confocal microscopy is a non-invasive tissue作者: 釘牢 時間: 2025-3-30 20:32 作者: 先行 時間: 2025-3-30 22:34 作者: 不吉祥的女人 時間: 2025-3-31 02:46
Cloning, Expression, and Purification of Galectins for In Vitro Studies,on of GST-galectins from glutathione-conjugated Sepharose with excess glutathione is both efficient and innocuous. The ability to bind and elute GST-galectins from lactose-conjugated Sepharose with excess lactose provides a relatively easy means to insure that galectins are competent for glycoconjug作者: barium-study 時間: 2025-3-31 08:58 作者: nascent 時間: 2025-3-31 10:40
,Introducing 77Se NMR Spectroscopy to Analyzing Galectin –Ligand Interaction,g. The documented strategic combination of synthetic carbohydrate chemistry and NMR spectroscopy prompts to envision to work with isotopically pure .Se-containing β-galactosides and to build on the gained experience with .Se by adding .F as second sensor in doubly labeled glycosides.作者: 點燃 時間: 2025-3-31 17:09
Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein,they formed reversible noncovalent cross-linked complexes with the lectin. Binding of sulfated GAGs to Gal-3 was completely inhibited when Gal-3 was preincubated with β-lactose. Cross-linking of Gal-3 by CSA, CSC, and the bovine CSPG was also reversed by β-lactose. These findings strongly suggest th作者: mortgage 時間: 2025-3-31 18:06
Mechanism of Mucin Recognition by Lectins : A Thermodynamic Study,bohydrate to carbohydrate epitope in these molecules leading to increased affinity. Importantly, the mechanism of binding of lectins to mucins appears similar to that for a variety of protein ligands binding to DNA. Recent results also show that high-affinity lectin–mucin cross-linking interactions 作者: 過濾 時間: 2025-3-31 22:20
Method for Identifying Galectin Ligands on Lymphocyte Membrane Glycoproteins,N-glycan structures on CD16a isolated from primary NK cells of healthy human donors was recently reported. NK cell CD16a is glycosylated at five N-glycosylation sites, and two of the five sites are modified, almost exclusively, by N-glycans with multiple LacNAc repeats which can serve as ligands for
