作者: 優(yōu)雅 時(shí)間: 2025-3-21 22:03
,Newton and Leibniz – Giants and Opponents,rocesses including gene transcription, translation, replication, and genomic stability. G-quadruplexes are also found in nonhuman genomes, particularly those of human pathogens. Therefore, G-quadruplexes have emerged as a new class of molecular targets for drug development. In addition, there is con作者: 旋轉(zhuǎn)一周 時(shí)間: 2025-3-22 00:37
32nd Hemophilia Symposium Hamburg 2001topology. Now, with the advent of affordable, commercially sourced and purified synthetic DNA and RNA molecules suitable for immediate crystallization trials, and combined with the availability of specialized and validated crystallization screens, researchers can now undertake in-house crystallizati作者: BLAND 時(shí)間: 2025-3-22 06:03 作者: 音樂等 時(shí)間: 2025-3-22 10:21
,Beendigung des Arbeitsverh?ltnisses,rase is incapable to resolve G-quadruplex structure and pauses at 3′-end of the G-quadruplex forming region when it transverses to the 5′-end of the template. Results from the DNA polymerase stop assay can provide the basis for further studies aimed at elucidating the major G-quadruplexes formed by 作者: fulcrum 時(shí)間: 2025-3-22 13:14 作者: fulcrum 時(shí)間: 2025-3-22 18:36
,Sicht I: Die Weit-Sicht des Vision?rs, and have been linked to regulation of gene expression in cancer and other diseases. Although molecules that bind to G4s, with subsequent influence on gene expression, have been well documented, the identification of new chemical scaffolds that potently and selectively bind to G4s and control specif作者: 自然環(huán)境 時(shí)間: 2025-3-23 01:03 作者: epicardium 時(shí)間: 2025-3-23 01:29 作者: 好色 時(shí)間: 2025-3-23 06:10
Electrophoretic Mobility Shift Assay and Dimethyl Sulfate Footprinting for Characterization of G-Quation of G-quadruplexes, which can be used to deduce the guanine bases involved in the formation of G-tetrads under physiological salt conditions. DMS footprinting can also reveal important information in G-quadruplex-protein complexes on protein contacts and regional changes in DNA G-quadruplex upo作者: Relinquish 時(shí)間: 2025-3-23 19:57 作者: 揉雜 時(shí)間: 2025-3-23 22:50
1064-3745 and experienced researchers who work in biophysics, structural biology, computational biology, biochemistry, and molecular and cell biology, and who want to learn more about the potential roles and effects of G-quadruplex in these fields.?. ..978-1-4939-9668-1978-1-4939-9666-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 航海太平洋 時(shí)間: 2025-3-24 03:14 作者: 水槽 時(shí)間: 2025-3-24 08:35 作者: 我正派 時(shí)間: 2025-3-24 13:03
,Newton and Leibniz – Giants and Opponents,-rich DNA and RNA sequences, G-quadruplexes can readily form under physiologically relevant conditions and are globularly folded structures. DNA is widely recognized as a double-helical structure essential in genetic information storage. However, only ~3% of the human genome is expressed in protein;作者: peritonitis 時(shí)間: 2025-3-24 16:02
A. Huth-Kühne,P. Lages,R. Zimmermannonal properties of nucleic acids. The reason is its high sensitivity to even slight changes in mutual orientation of absorbing bases of DNA. CD can reveal formation of particular structural DNA arrangements and can be used to search for the conditions stabilizing the structures, to follow the transi作者: 老人病學(xué) 時(shí)間: 2025-3-24 21:16
Gabi Ben-Dor,Oren Sadot,Ozer Igraptimization of potent G-quadruplex ligands for use in therapeutics, diagnostics, or other technological applications. Isothermal titration calorimetry (ITC) can resolve energetic contributions to complex formation and constitutes the only available experimental method to directly measure binding ent作者: CORE 時(shí)間: 2025-3-25 01:11
A.E. Lutsky,I.V. Mursenkova,I.A. Znamenskayareal time, without the need for labeling any components of the interaction. The method provides real-time equilibrium and kinetic characterization for quadruplex DNA interactions and requires small amounts of materials and no external probe. A detailed protocol for quadruplex-DNA interaction analyse作者: 行乞 時(shí)間: 2025-3-25 06:29 作者: 修改 時(shí)間: 2025-3-25 08:45
R. Zhou,B. L. Tian,X. P. Li,J. P. Wangcterized thermodynamically, providing the information on the stability of various G4 conformations. We show how the relevant thermodynamic and sometimes kinetic parameters are obtained by employing differential scanning calorimetry (DSC) and global fitting of an appropriate model to the DSC data. We作者: anniversary 時(shí)間: 2025-3-25 13:44 作者: craven 時(shí)間: 2025-3-25 17:30 作者: 四溢 時(shí)間: 2025-3-25 22:24 作者: 對(duì)手 時(shí)間: 2025-3-26 00:17 作者: 人類 時(shí)間: 2025-3-26 07:38 作者: 步履蹣跚 時(shí)間: 2025-3-26 10:57 作者: Polydipsia 時(shí)間: 2025-3-26 16:35 作者: Palter 時(shí)間: 2025-3-26 17:53 作者: CRATE 時(shí)間: 2025-3-26 23:35 作者: 修改 時(shí)間: 2025-3-27 03:54
35th Hemophilia Symposium Hamburg 2004Q)- and counterpart i-motif-forming sequences in the DNA nanostructure. Sequential manipulation of DNA strands in the DNA frame was performed to prepare a topologically controlled GQ/i-motif dsDNA. Using the strand displacement and the addition and removal of K., the topologically controlled GQ/i-mo作者: 鉗子 時(shí)間: 2025-3-27 07:02
https://doi.org/10.1007/978-90-313-8424-2rming sequences exist throughout the genome and G4 structures are shown to be involved in many processes including DNA replication and gene expression. The single-molecule total internal reflection fluorescence (TIRF) microscopy has been employed to study G4 structure formation and protein binding i作者: BALK 時(shí)間: 2025-3-27 10:27
,Sicht I: Die Weit-Sicht des Vision?rs,cceptor molecule when the donor and acceptor are in close proximity to each other. Depending on the assay design, FRET can provide a real-time measurement of structural integrity and dynamics of biomacromolecules in solution and is particularly suitable for studying G-quadruplex (G4) nucleic acids a作者: 樣式 時(shí)間: 2025-3-27 13:56
A. Calatzis,W. Schramm,M. SpannaglAnalytical ultracentrifugation is a powerful biophysical tool that provides information about G-quadruplex structure, stability, and binding reactivity. This chapter provides a simplified explanation of the method, along with examples of how it can be used to characterize G4 formation and to monitor small-molecule binding.作者: 原諒 時(shí)間: 2025-3-27 20:33 作者: 易彎曲 時(shí)間: 2025-3-27 22:36 作者: Texture 時(shí)間: 2025-3-28 05:21
G-Quadruplex Nucleic Acids978-1-4939-9666-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: languor 時(shí)間: 2025-3-28 09:46 作者: 光亮 時(shí)間: 2025-3-28 13:42
Quantitative Analysis of Stall of Replicating DNA Polymerase by G-Quadruplex Formation,he human genome exhibit G4-forming potential, the stability and topology of the G4s formed vary depending on the molecular environment. Here, we describe a protocol to quantitatively analyze the inhibitory effects of G4s with different stabilities and topologies on replication in conditions of molecular crowding.作者: conspicuous 時(shí)間: 2025-3-28 16:32
G-Quadruplex DNA and RNA,-rich DNA and RNA sequences, G-quadruplexes can readily form under physiologically relevant conditions and are globularly folded structures. DNA is widely recognized as a double-helical structure essential in genetic information storage. However, only ~3% of the human genome is expressed in protein;作者: conference 時(shí)間: 2025-3-28 21:52 作者: homocysteine 時(shí)間: 2025-3-29 00:57
Revealing the Energetics of Ligand-Quadruplex Interactions Using Isothermal Titration Calorimetry,ptimization of potent G-quadruplex ligands for use in therapeutics, diagnostics, or other technological applications. Isothermal titration calorimetry (ITC) can resolve energetic contributions to complex formation and constitutes the only available experimental method to directly measure binding ent作者: 表皮 時(shí)間: 2025-3-29 06:09
Biosensor-Surface Plasmon Resonance: Label-Free Method for Investigation of Small Molecule-Quadruplreal time, without the need for labeling any components of the interaction. The method provides real-time equilibrium and kinetic characterization for quadruplex DNA interactions and requires small amounts of materials and no external probe. A detailed protocol for quadruplex-DNA interaction analyse作者: etiquette 時(shí)間: 2025-3-29 10:09 作者: Pericarditis 時(shí)間: 2025-3-29 11:40
G-Quadruplex Stability from DSC Measurements,cterized thermodynamically, providing the information on the stability of various G4 conformations. We show how the relevant thermodynamic and sometimes kinetic parameters are obtained by employing differential scanning calorimetry (DSC) and global fitting of an appropriate model to the DSC data. We作者: 通便 時(shí)間: 2025-3-29 17:42
X-Ray Crystallographic Studies of G-Quadruplex Structures, researchers with exciting opportunities to explore new structural arrangements of putative G4 forming sequences and investigate their recognition by small molecule compounds. The crowded and ordered crystalline environment requires the self-assembly of stable G4 motifs, allowing for an understandin作者: Aqueous-Humor 時(shí)間: 2025-3-29 21:07
NMR Studies of G-Quadruplex Structures and G-Quadruplex-Interactive Compounds,quadruplex structures have been found to form in nucleic acid regions of biological significance, including human telomeres, gene promoters, and untranslated regions of mRNA. Thus, they are considered attractive therapeutic targets. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method 作者: 遷移 時(shí)間: 2025-3-30 01:57
Using Molecular Dynamics Free Energy Simulation to Compute Binding Affinities of DNA G-Quadruplex LNA. Such calculations have a number of applications, such as rescoring docking results and validating docked poses, to inform the discovery of G-quadruplex binders with high affinity and selectivity. This chapter describes two binding free energy protocols: the double decoupling method (DDM) and the作者: 歌曲 時(shí)間: 2025-3-30 05:15
Electrophoretic Mobility Shift Assay and Dimethyl Sulfate Footprinting for Characterization of G-Quumulating evidence supporting G-quadruplex involvement in a number of important aspects of genome functions, including transcription, replication, and genomic stability, and that protein and enzyme recognition of G-quadruplexes may represent a key event to regulate physiological or pathological path作者: mercenary 時(shí)間: 2025-3-30 09:44 作者: Flatter 時(shí)間: 2025-3-30 14:03
Chromatin Immunoprecipitation Assay to Analyze the Effect of G-Quadruplex Interactive Agents on these studies also provide strong evidence that G-quadruplex structures are stabilized by binding to small molecules, resulting in the modulation of the transcription of genes whose promoters form G-quadruplexes. Here, we describe a chromatin immunoprecipitation (ChIP) assay to determine whether G-quad作者: 一瞥 時(shí)間: 2025-3-30 17:21 作者: FADE 時(shí)間: 2025-3-30 21:19 作者: Innocence 時(shí)間: 2025-3-31 03:12
Single-Molecule Investigations of G-Quadruplex,ion kinetics of the structures. Although the core structural element in a G-quadruplex is simple and requires only four tandem repeats of Guanine rich sequences, there is rather rich conformational diversity in this structure. Corresponding to this structural diversity, it displays involved transiti作者: Crayon 時(shí)間: 2025-3-31 05:30 作者: 東西 時(shí)間: 2025-3-31 10:41
G-Quadruplex and Protein Binding by Single-Molecule FRET Microscopy,rming sequences exist throughout the genome and G4 structures are shown to be involved in many processes including DNA replication and gene expression. The single-molecule total internal reflection fluorescence (TIRF) microscopy has been employed to study G4 structure formation and protein binding i作者: Debate 時(shí)間: 2025-3-31 14:00 作者: 浮雕寶石 時(shí)間: 2025-3-31 19:31 作者: 針葉 時(shí)間: 2025-3-31 23:16 作者: oncologist 時(shí)間: 2025-4-1 02:43 作者: 能夠支付 時(shí)間: 2025-4-1 09:28
Eric Johnsen,M. T. Henry de Frahan information of G-quadruplexes and their ligand-bound complexes (such as the cation/ligand binding stoichiometry, and the number of strands and G-quartets) can be obtained from a single spectrum using this ESI-MS-based method.作者: indices 時(shí)間: 2025-4-1 13:40 作者: 元音 時(shí)間: 2025-4-1 16:29
Biosensor-Surface Plasmon Resonance: Label-Free Method for Investigation of Small Molecule-Quadrupln, quantitative and qualitative data analyses, and presentation are described. Some specific examples of small molecule-DNA quadruplex interactions are presented with results evaluated by both kinetic and steady-state SPR methods.作者: Insulin 時(shí)間: 2025-4-1 19:24
Mass Spectroscopic Study of G-Quadruplex, information of G-quadruplexes and their ligand-bound complexes (such as the cation/ligand binding stoichiometry, and the number of strands and G-quartets) can be obtained from a single spectrum using this ESI-MS-based method.作者: Concrete 時(shí)間: 2025-4-2 00:23 作者: 公理 時(shí)間: 2025-4-2 05:38 作者: AGGER 時(shí)間: 2025-4-2 07:34
