標題: Titlebook: G Protein-Coupled Receptors in Drug Discovery; Methods and Protocol Marta Filizola Book 2015Latest edition Springer Science+Business Media [打印本頁] 作者: Aggrief 時間: 2025-3-21 16:43
書目名稱G Protein-Coupled Receptors in Drug Discovery影響因子(影響力)
書目名稱G Protein-Coupled Receptors in Drug Discovery影響因子(影響力)學科排名
書目名稱G Protein-Coupled Receptors in Drug Discovery網(wǎng)絡公開度
書目名稱G Protein-Coupled Receptors in Drug Discovery網(wǎng)絡公開度學科排名
書目名稱G Protein-Coupled Receptors in Drug Discovery被引頻次
書目名稱G Protein-Coupled Receptors in Drug Discovery被引頻次學科排名
書目名稱G Protein-Coupled Receptors in Drug Discovery年度引用
書目名稱G Protein-Coupled Receptors in Drug Discovery年度引用學科排名
書目名稱G Protein-Coupled Receptors in Drug Discovery讀者反饋
書目名稱G Protein-Coupled Receptors in Drug Discovery讀者反饋學科排名
作者: 生氣的邊緣 時間: 2025-3-21 23:03 作者: acolyte 時間: 2025-3-22 02:29 作者: 草本植物 時間: 2025-3-22 06:58
Design of Digital Campus Based on WebGISf the conformational landscape and are involved in activation. Through relaxation experiments spanning microseconds to seconds, lifetimes of these functional states can often be measured. By determining the effect of ligands on both equilibrium populations and rates of interconversion between states作者: 鍍金 時間: 2025-3-22 10:19 作者: 類型 時間: 2025-3-22 13:20
https://doi.org/10.1007/978-3-642-76764-7alculations. Several examples are discussed that illustrate specific steps that can be taken to improve upon the docking and virtual screening accuracy. While GPCRs are a unique target class, many of the methods and strategies outlined in this review are general and therefore applicable to other pro作者: 類型 時間: 2025-3-22 19:15
Cuddling and Spooning Other Menl, while other types of data are more useful at the stage of solution filtering. The protocol was successfully applied to modeling and design of a stable construct that resulted in crystallization of the first complex between a chemokine and its receptor. Examples from this work are used to illustra作者: CRACY 時間: 2025-3-23 00:32
Purification of Stabilized GPCRs for Structural and Biophysical Analyses,tructural and biophysical studies. Example protocols for the purification of StaR proteins for analysis, ligand screening with the thiol-specific fluorochrome .-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM), surface plasmon resonance (SPR), and crystallization for structural studie作者: 蛤肉 時間: 2025-3-23 04:18 作者: 刺穿 時間: 2025-3-23 06:03 作者: 繁榮地區(qū) 時間: 2025-3-23 10:03 作者: Campaign 時間: 2025-3-23 16:55
,Experiment-Guided Molecular Modeling of Protein–Protein Complexes Involving GPCRs,l, while other types of data are more useful at the stage of solution filtering. The protocol was successfully applied to modeling and design of a stable construct that resulted in crystallization of the first complex between a chemokine and its receptor. Examples from this work are used to illustra作者: 縮短 時間: 2025-3-23 20:09 作者: Critical 時間: 2025-3-23 23:58 作者: 詢問 時間: 2025-3-24 04:42 作者: 保留 時間: 2025-3-24 09:54 作者: 進取心 時間: 2025-3-24 12:41
https://doi.org/10.1007/978-981-97-0206-0nd .. a measure of affinity) allows the identification of agonists that demonstrate unique signaling either for different signaling pathways linked to the receptor, differences in cellular host, effects of receptor mutation, or receptor selectivity profiles. The procedure includes statistical methods to assess the significance of these effects.作者: PRO 時間: 2025-3-24 17:47 作者: Enliven 時間: 2025-3-24 20:43
21. Deutscher Soziologentag 1982ciated with exon 11. These exon 11-associated truncated variants are not readily labeled with current radioligands. Here we describe the synthesis of a radioiodinated ligand suitable for carrying out binding studies for this target.作者: 劇毒 時間: 2025-3-25 00:33
Bruno S. Frey,Christoph A. Schalteggerunable to discriminate between different, yet energetically similar, poses because of their relatively simple scoring functions. Here, we describe a metadynamics-based approach to study the dynamic process of ligand binding to/unbinding from GPCRs with a higher level of accuracy and yet satisfying efficiency.作者: 綁架 時間: 2025-3-25 06:59 作者: cruise 時間: 2025-3-25 07:36 作者: EVADE 時間: 2025-3-25 15:43
Detection and Quantification of Intracellular Signaling Using FRET-Based Biosensors and High Content FRET biosensors to measure cAMP levels, kinase (ERK and PKC), and GTPase activity. Importantly, we provide the protocols to express and measure these sensors in a variety of model cell lines and primary dorsal root ganglia neurons.作者: 生意行為 時間: 2025-3-25 17:59 作者: 多產(chǎn)子 時間: 2025-3-25 23:59
Bioluminescence Resonance Energy Transfer Approaches to Discover Bias in GPCR Signaling,e molecules of interest. As BRET is readily applied to the study of numerous GPCR signaling and regulatory paths, it is an ideal technique for investigating the pharmacology of biased ligands and receptors.作者: Ataxia 時間: 2025-3-26 00:13
Radioligand Binding Assay for an Exon 11-Associated Mu Opioid Receptor Target,ciated with exon 11. These exon 11-associated truncated variants are not readily labeled with current radioligands. Here we describe the synthesis of a radioiodinated ligand suitable for carrying out binding studies for this target.作者: 巫婆 時間: 2025-3-26 06:00 作者: 壓艙物 時間: 2025-3-26 09:32 作者: Mirage 時間: 2025-3-26 15:45
Regular Press Conference (July)g of GPCRs with bright organic fluorophores and fluorescent imaging by total internal reflection fluorescence microscopy. Using this method, individual tracks of single molecules can be analyzed in parallel with high spatial precision and with frame rates up to 50/s.作者: beta-cells 時間: 2025-3-26 20:17
https://doi.org/10.1007/978-3-030-75401-3 application to determine not only protein–protein interactions but also the capability of GPCR mutant variants to form homomers compared to the wild type GPCR within the plasma membrane of transfected cells.作者: 抱負 時間: 2025-3-26 21:30
https://doi.org/10.1007/978-3-658-30820-9esents our method and offers tips for evaluating G protein signaling in endogenous tissues. Predominately, brain tissues are discussed here; optimization points that can be applied to any tissues are highlighted.作者: 侵害 時間: 2025-3-27 02:08
https://doi.org/10.1007/978-3-658-08454-7in this chapter can be applied to other members of the DREADD family and, of course, different cell types. It is likely that the use of DREADD technology will identify physiologically important signaling pathways that can be targeted for therapeutic purposes.作者: 墻壁 時間: 2025-3-27 06:33 作者: 乳汁 時間: 2025-3-27 09:58 作者: 癡呆 時間: 2025-3-27 16:40 作者: adequate-intake 時間: 2025-3-27 18:12 作者: MELD 時間: 2025-3-28 00:56
High-Throughput Screening for Allosteric Modulators of GPCRs,ram. The identification of allosteric modulators, in particular, poses significant challenges for HTS. We describe several HTS protocols designed for the identification of GPCR ligands, with a particular focus on the identification of allosteric modulators.作者: 樹木心 時間: 2025-3-28 03:21
2001 Mars Odyssey Mission Summary,ation on the overall architecture and dynamics of protein complexes. Here we provide a concise protocol for negative stain imaging and two-dimensional (2D) projection analysis of GPCR complexes, including notes for the intricacies of the application in these biological systems.作者: 一個姐姐 時間: 2025-3-28 09:13 作者: 充滿人 時間: 2025-3-28 13:54
2D Projection Analysis of GPCR Complexes by Negative Stain Electron Microscopy,ation on the overall architecture and dynamics of protein complexes. Here we provide a concise protocol for negative stain imaging and two-dimensional (2D) projection analysis of GPCR complexes, including notes for the intricacies of the application in these biological systems.作者: allergen 時間: 2025-3-28 16:34 作者: obstinate 時間: 2025-3-28 19:28 作者: dry-eye 時間: 2025-3-28 23:34 作者: 假裝是我 時間: 2025-3-29 03:11
Messner Mountain Museum ?Ortles“, Suldented in the structure determination of more than 25 GPCRs, including representatives from class A, B, C, and F. Most of the X-ray structures available correspond to an inactive conformation of the receptor bound to an antagonist. Only a few high-resolution structures of agonist-bound conformations of作者: 刪減 時間: 2025-3-29 08:03 作者: 尾隨 時間: 2025-3-29 13:59 作者: effrontery 時間: 2025-3-29 18:59 作者: 民間傳說 時間: 2025-3-29 21:11 作者: 輕快走過 時間: 2025-3-30 01:29 作者: 愛好 時間: 2025-3-30 04:20 作者: erythema 時間: 2025-3-30 10:40
https://doi.org/10.1007/978-981-16-9061-7or. Fluorescence indicators of different sizes and chemical characteristics can provide insights into the nature of the binding environment, the surrounding structures, and even into conformational changes associated with receptor activation. Methods for determining fluorescence spectral analysis, f作者: Collision 時間: 2025-3-30 14:34
Ning Jiang,Wenbin Li,Weihang Li,Dou Hong intracellular signaling that cannot be attained using traditional methods. Here, we describe a detailed protocol for the use of high content imaging in combination with FRET biosensors to assess second messenger production and intracellular signaling in a time-effective manner. We use four differen作者: 恩惠 時間: 2025-3-30 19:18
https://doi.org/10.1007/978-981-97-0206-0 biased profiles. Biased ligands have different pharmacological properties on a molecular level (stabilization of different receptor active states) and thus can have different pharmacological profiles therapeutically. The calculation of transduction ratios (ΔΔlog(./..)) values (where . is efficacy a作者: 獨行者 時間: 2025-3-31 00:06 作者: exorbitant 時間: 2025-3-31 01:34 作者: 樂器演奏者 時間: 2025-3-31 07:25
https://doi.org/10.1007/978-3-658-08454-7signer .rugs) have emerged as powerful new tools for the study of GPCR physiology. In this chapter, we present protocols employing adeno-associated viruses (AAVs) to express a G.-coupled DREADD (Dq) in two metabolically important cell types, AgRP neurons of the hypothalamus and hepatocytes of the li作者: Albinism 時間: 2025-3-31 11:59 作者: 手術刀 時間: 2025-3-31 16:05
21. Deutscher Soziologentag 1982u, delta, and kappa. Highly selective radioligands are available for all three classes of traditional receptors. Of the three, the mu receptor undergoes extensive alternative splicing, generating a number of traditional mu receptor subtypes as well as a nontraditional, truncated set of variants asso作者: 甜瓜 時間: 2025-3-31 18:06
https://doi.org/10.1007/978-3-642-76764-7 this pharmaceutically important target class. The quality of GPCR structures available for SBDD projects fall on a spectrum ranging from high resolution crystal structures (<2 ?), where all water molecules in the binding pocket are resolved, to lower resolution (>3 ?) where some protein residues ar作者: reaching 時間: 2025-4-1 00:45 作者: grotto 時間: 2025-4-1 04:47
Cuddling and Spooning Other Men infancy. In the absence of complex structures, molecular modeling and docking play a large role not only by providing a proper 3D context for interpretation of biochemical and biophysical data, but also by prospectively guiding experiments. Experimentally confirmed restraints may help improve the a作者: indemnify 時間: 2025-4-1 06:01
https://doi.org/10.1007/978-1-4939-2914-6Allosteric modulators; Biased agonists; Drug design; Drug targets; GPCRs; Oligomer-selective ligands; Rece作者: Instinctive 時間: 2025-4-1 11:06
