作者: Inflammation 時(shí)間: 2025-3-21 22:19 作者: 聽覺 時(shí)間: 2025-3-22 01:01 作者: Implicit 時(shí)間: 2025-3-22 08:21 作者: Focus-Words 時(shí)間: 2025-3-22 09:00
https://doi.org/10.1007/978-3-0348-6378-0d to estimate the biological activity of various GPCR-specific agents. F?rster resonance energy transfer (FRET) biosensors have been around for almost 10 years and became increasingly popular for cAMP detection. Ratiometric sensitized emission assay of a FRET biosensor can easily be implemented on f作者: Immortal 時(shí)間: 2025-3-22 15:07 作者: Immortal 時(shí)間: 2025-3-22 17:03
Margret Liehn,Hannelore Schlautmannause their cognate G protein signaling pathways are often unknown. In this methodological chapter, we describe the use of constitutive activity, that is, the inherent ability of receptors to couple to their cognate G proteins in the absence of ligand, to inform the development of high-throughput scr作者: 法律 時(shí)間: 2025-3-22 23:27 作者: 萬靈丹 時(shí)間: 2025-3-23 01:40 作者: 偏離 時(shí)間: 2025-3-23 08:09 作者: 節(jié)省 時(shí)間: 2025-3-23 11:34
Minimising the Cause of Human Error to accurately and timely extract useful information from image and video data has become more and more important. Image J is an open-source program that provides powerful tools with a simple interface designed to fit the needs of image analysis of most researchers. In this chapter, Image J routines作者: 高度表 時(shí)間: 2025-3-23 17:26 作者: 很像弓] 時(shí)間: 2025-3-23 21:26 作者: Forage飼料 時(shí)間: 2025-3-24 01:06 作者: Favorable 時(shí)間: 2025-3-24 02:46
https://doi.org/10.1007/978-3-658-24813-0es, autoimmune diseases, inflammation, cancer, and diseases of the nervous system. Moreover, they are one of the most amenable targets for drug discovery because they can be modulated by small molecules, peptides, proteins, and antibodies. Therefore it may not come as a surprise that close to 40 % o作者: 預(yù)示 時(shí)間: 2025-3-24 07:06
https://doi.org/10.1007/978-3-658-01505-3ssibility for discovering GPCR drugs with better efficacy and safety profiles. However, quantification of ligand bias is challenging. Herein, we present five different label-free dynamic mass redistribution (DMR) approaches to assess ligand bias acting at the β.-adrenergic receptor (β.AR). Multipara作者: 收藏品 時(shí)間: 2025-3-24 14:28 作者: LITHE 時(shí)間: 2025-3-24 15:33 作者: Nerve-Block 時(shí)間: 2025-3-24 20:47
978-1-4939-5580-0Springer Science+Business Media New York 2015作者: 極深 時(shí)間: 2025-3-25 03:08 作者: 倔強(qiáng)不能 時(shí)間: 2025-3-25 06:48 作者: AGATE 時(shí)間: 2025-3-25 09:03 作者: GRAZE 時(shí)間: 2025-3-25 12:21 作者: thalamus 時(shí)間: 2025-3-25 17:11
Christoph Keese,Wolfgang Münchauintracellular calcium changes. The assay is designed to study calcium mobilization induced by majority of GPCRs and calcium channels and allows for simultaneous concentration-dependent analysis of several receptor agonists and antagonists, useful in receptor characterization and drug discovery projects.作者: Hemodialysis 時(shí)間: 2025-3-25 20:35 作者: transient-pain 時(shí)間: 2025-3-26 01:09 作者: 燒瓶 時(shí)間: 2025-3-26 05:24
Solutions to Introductory Problems,ods – can become extremely powerful approaches for visualizing these cellular protein–protein interactions, even between more than two proteins. Overall, these methods might become extremely important tools in GPCR drug discovery.作者: Narrative 時(shí)間: 2025-3-26 09:03
Minimising the Cause of Human Errorcan be separated using either a progressive threshold or a local threshold method. Both methods can be optimized to either a maximum number of selection or maximum area selected resulting in conceptually distinct selections.作者: BANAL 時(shí)間: 2025-3-26 13:49 作者: AER 時(shí)間: 2025-3-26 19:12 作者: 放肆的你 時(shí)間: 2025-3-26 22:01
Quantifying GPCR Internalization: A Focus on the Kisspeptin Receptorhe use of immunofluorescence and imaging techniques as well as flow cytometry. The techniques described use the FLAG-tagged kisspeptin receptor (KISS1R) as an example but are equally applicable to any other GPCR.作者: Provenance 時(shí)間: 2025-3-27 01:16 作者: 喃喃訴苦 時(shí)間: 2025-3-27 05:52 作者: Seizure 時(shí)間: 2025-3-27 13:30
Measurement of Surface-Mediated Ca2+ Transients on the Single-Cell Level in a Microfluidic Lab-on-a-er cells or particles, and the simultaneous analysis of the induced cytosolic calcium signals. The method is based on a microfluidic lab-on-a-chip environment that allows for contactless cell and particle handling by dielectrophoretic forces.作者: 增強(qiáng) 時(shí)間: 2025-3-27 14:09
https://doi.org/10.1007/978-3-0348-6378-0luorescence plate reader platforms. For such assays a considerable amount of cells expressing the desired biosensor is needed. A method to achieve sufficient and reproducible level of cAMP biosensor protein expression with the means of BacMam transduction system is the subject of this chapter.作者: modest 時(shí)間: 2025-3-27 18:13
cAMP Assay for GPCR Ligand Characterization: Application of BacMam Expression Systemluorescence plate reader platforms. For such assays a considerable amount of cells expressing the desired biosensor is needed. A method to achieve sufficient and reproducible level of cAMP biosensor protein expression with the means of BacMam transduction system is the subject of this chapter.作者: 割公牛膨脹 時(shí)間: 2025-3-27 22:34 作者: crescendo 時(shí)間: 2025-3-28 05:34 作者: 獨(dú)特性 時(shí)間: 2025-3-28 10:08
https://doi.org/10.1007/978-3-658-24813-0 activity integrates this complexity. In this assay protocol, we describe how the xCELLigence RTCA HT impedance-based platform which can be used for functional cell-based GPCR assays can be utilized for GPCR screening.作者: Minutes 時(shí)間: 2025-3-28 13:54
https://doi.org/10.1007/978-3-658-01505-3gand-directed desensitization of the receptor. DMR antagonist reverse assays manifest biased antagonism. DMR profiling using distinct probe-modulated cells detects the biased agonism in the context of self-referenced pharmacological activity map.作者: 慢跑 時(shí)間: 2025-3-28 15:38 作者: 組成 時(shí)間: 2025-3-28 19:54
Olfactory Receptor Screening Assay Using Nanovesicle-Immobilized Carbon Nanotube Transistor surface membrane and induce influx of calcium ions similar to olfactory signal transduction. This ion influx causes an electrical current change along the carbon nanotube, and then this change is measured by the SWNT-FET sensor. This technique facilitates the simple and rapid screening of OR functions.作者: 蝕刻術(shù) 時(shí)間: 2025-3-28 23:18 作者: 背心 時(shí)間: 2025-3-29 05:15 作者: 亞當(dāng)心理陰影 時(shí)間: 2025-3-29 10:03 作者: 包庇 時(shí)間: 2025-3-29 14:53
G protein-Coupled Receptors: An Overview of Signaling Mechanisms and Screening Assaysof the most exciting aspects of cell biology. Among the different receptor types recognized today, G-protein-coupled receptors (GPCRs) constitute, undoubtedly, one of the most important classes, in part due to their versatility, but particularly, due to their central role in a multitude of physiolog作者: 陳腐的人 時(shí)間: 2025-3-29 19:13 作者: 有角 時(shí)間: 2025-3-29 21:37 作者: 變白 時(shí)間: 2025-3-30 02:28 作者: Hyperlipidemia 時(shí)間: 2025-3-30 05:51 作者: Tailor 時(shí)間: 2025-3-30 09:28
Ca2+ Mobilization Assays in GPCR Drug Discoveryfluorescence-based method (FLIPR Calcium 4 Assay) developed by Molecular Devices for a FlexStation and routinely used in our laboratory for detecting intracellular calcium changes. The assay is designed to study calcium mobilization induced by majority of GPCRs and calcium channels and allows for si作者: 隱語 時(shí)間: 2025-3-30 16:20 作者: 繁榮中國 時(shí)間: 2025-3-30 18:53
Monitoring G Protein-Coupled Receptor Activation Using the Protein Fragment Complementation Techniqu, proliferation, hormonal regulation, and neuronal activity. Further, they are involved in many disease-relevant processes, such as cancer and neurodevelopmental diseases, and represent the largest class of drug targets. Therefore, monitoring how GPCRs are regulated in their activity is crucial to u作者: RAG 時(shí)間: 2025-3-30 23:34 作者: oracle 時(shí)間: 2025-3-31 01:25 作者: 鬼魂 時(shí)間: 2025-3-31 07:57
Use of ImageJ to Recover Information from Individual Cells in a G Protein-Coupled Receptor Assay to accurately and timely extract useful information from image and video data has become more and more important. Image J is an open-source program that provides powerful tools with a simple interface designed to fit the needs of image analysis of most researchers. In this chapter, Image J routines作者: 谷類 時(shí)間: 2025-3-31 10:25
