標(biāo)題: Titlebook: G Protein Signaling; Methods and Protocol Alan V. Smrcka Book 2004 Humana Press 2004 [打印本頁(yè)] 作者: legerdemain 時(shí)間: 2025-3-21 17:36
書(shū)目名稱G Protein Signaling影響因子(影響力)
書(shū)目名稱G Protein Signaling影響因子(影響力)學(xué)科排名
書(shū)目名稱G Protein Signaling網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱G Protein Signaling網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱G Protein Signaling被引頻次
書(shū)目名稱G Protein Signaling被引頻次學(xué)科排名
書(shū)目名稱G Protein Signaling年度引用
書(shū)目名稱G Protein Signaling年度引用學(xué)科排名
書(shū)目名稱G Protein Signaling讀者反饋
書(shū)目名稱G Protein Signaling讀者反饋學(xué)科排名
作者: Ceremony 時(shí)間: 2025-3-22 00:06 作者: 熟練 時(shí)間: 2025-3-22 01:34 作者: PALL 時(shí)間: 2025-3-22 07:54 作者: 漸變 時(shí)間: 2025-3-22 11:19 作者: Contracture 時(shí)間: 2025-3-22 16:51
Book 2004ucture, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent 作者: Contracture 時(shí)間: 2025-3-22 17:04
Expression and Purification of Soluble Adenylyl Cyclase from ,histidine tag (H6) is incorporated into the expression vector and utilized for affinity purification on a Ni-NTA column. Subsequently, an anion exchange column is employed to further purify the protein.作者: 青石板 時(shí)間: 2025-3-22 21:59
Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cellsbecause AC that are endogenously expressed in Sf9 cells contribute low amounts of activity relative to the overexpressed enzyme. These approaches are applicable to all nine isoforms of mammalian membrane-bound AC isoforms.作者: cortisol 時(shí)間: 2025-3-23 03:23
Analysis of G Protein-Mediated Activation of Phospholipase C in Cultured Cellsibes a cell-based assay system to determine the activity of PLC by monitoring the levels of IPs in cultured cells. This assay system can be used to examine the activation of G protein-coupled receptors (GPCRs), coupling of GPCRs to G proteins, and the interactions of G proteins to PLCβ.作者: Wernickes-area 時(shí)間: 2025-3-23 06:10
Analysis of the Coupling of G12/13 to G Protein-Coupled Receptors Using a Luciferase Reporter Assay of the reporter luciferase under the control of a modified serum-responsive element (SRE) in a cell line derived from mice lacking Gα. that are transfected with a GPCR of interest, allows for easy, fast, and valid determination of the coupling of the GPCR to Gα..作者: Lobotomy 時(shí)間: 2025-3-23 11:14
Selective Inhibition of G Protein-Mediated Pathways Using RGS Domainss through RGS domain of about 120 amino acids. Thus, the overexpression of the RGS domain in cells can specifically block the signalling pathways that are mediated by the interacting G proteins. The method is particularly useful to differentiate between G.-mediated and G.-mediated pathways.作者: 舊式步槍 時(shí)間: 2025-3-23 17:21
Carol Anne Spreen,Chrissie Monaghan)-tagged subunits using metal chelate chromatography. Modification of Gα with myristate can be recapitulated in . by expressing .-myristoyltransferase (NMT) with its G protein substrate. Protocols for the production and purification of myristoylated Gα are presented.作者: TIA742 時(shí)間: 2025-3-23 21:39
From Tre Styles to Trayvon Martin)-tagged PLC β the purification involves two steps, affinity chromatography with Ni-NTA agarose followed by heparin Sepharose chromatography. 6-His-tagged PLCε can be purified in a single step with nickel nitrilotriacetic acid-agarose (Ni-NTA) affinity chromatography.作者: TOXIN 時(shí)間: 2025-3-24 01:11
Incivility as a Tool for Social Change,tion of a phosphatidylalcohol, which is a specific and unique marker for PLD activity. This protocol is highly sensitive for the detection of PLD activity following the stimulation of intact cells, being a valuable method for studying the regulation of PLD activity in vivo.作者: 凝結(jié)劑 時(shí)間: 2025-3-24 06:02 作者: 欺騙世家 時(shí)間: 2025-3-24 07:12 作者: Fortify 時(shí)間: 2025-3-24 12:04 作者: 冰雹 時(shí)間: 2025-3-24 15:20 作者: 吸氣 時(shí)間: 2025-3-24 19:51 作者: Headstrong 時(shí)間: 2025-3-24 23:15 作者: 顛簸下上 時(shí)間: 2025-3-25 03:40
1064-3745 f physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole anima作者: Digest 時(shí)間: 2025-3-25 08:27
https://doi.org/10.1007/978-94-6091-734-9histidine tag (H6) is incorporated into the expression vector and utilized for affinity purification on a Ni-NTA column. Subsequently, an anion exchange column is employed to further purify the protein.作者: GLUT 時(shí)間: 2025-3-25 12:22 作者: 職業(yè)拳擊手 時(shí)間: 2025-3-25 18:49 作者: deactivate 時(shí)間: 2025-3-25 22:53
De fysiologie van de glucosehuishouding, of the reporter luciferase under the control of a modified serum-responsive element (SRE) in a cell line derived from mice lacking Gα. that are transfected with a GPCR of interest, allows for easy, fast, and valid determination of the coupling of the GPCR to Gα..作者: 聯(lián)合 時(shí)間: 2025-3-26 02:40
https://doi.org/10.1007/978-3-322-97190-6s through RGS domain of about 120 amino acids. Thus, the overexpression of the RGS domain in cells can specifically block the signalling pathways that are mediated by the interacting G proteins. The method is particularly useful to differentiate between G.-mediated and G.-mediated pathways.作者: 動(dòng)機(jī) 時(shí)間: 2025-3-26 04:58 作者: 哪有黃油 時(shí)間: 2025-3-26 10:05
Assay for G Protein-Dependent Activation of Phospholipase C β Using Purified Protein Componentsivity PLC activity and its ability to be regulated by βγ and α. subunits. It can also be used to assess the functionality of the components after modification by mutagenesis, chemical modification, or in the presence of competing molecules.作者: 食物 時(shí)間: 2025-3-26 13:14 作者: MORPH 時(shí)間: 2025-3-26 20:08 作者: nitric-oxide 時(shí)間: 2025-3-26 21:04
G Protein Signaling978-1-59259-430-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Exclaim 時(shí)間: 2025-3-27 01:24 作者: 不感興趣 時(shí)間: 2025-3-27 08:02 作者: hypnotic 時(shí)間: 2025-3-27 10:04 作者: Conduit 時(shí)間: 2025-3-27 17:32
Carol Anne Spreen,Chrissie Monaghanin for biochemical and biophysical analyses. Wild-type and mutant forms of Gα are easily produced for analysis of their intrinsic biochemical properties, as well as for reconstitution with receptors, effectors, regulators, and G protein βγ subunits. Methods are described for the expression of G.α an作者: Intersect 時(shí)間: 2025-3-27 21:04
Yukiko Yamaguchi,Nikhilesh Dholakiacombinant G proteins are critically important. Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits.作者: beta-carotene 時(shí)間: 2025-3-27 22:57 作者: 易于出錯(cuò) 時(shí)間: 2025-3-28 04:24
From Tre Styles to Trayvon Martinerol (DAG) and inositol 1,4,5-triphosphate (IP.). The PLCβ isoforms of PLCs are activated by G proteins after hormone or neurotransmitter stimulation of G protein-coupled receptors (GPCR). PLCe is a recently identified PLC isoform that is activated by Ras and Gβγ subunit although the physiological r作者: transdermal 時(shí)間: 2025-3-28 10:06 作者: Commodious 時(shí)間: 2025-3-28 10:50
https://doi.org/10.1007/978-3-662-09790-8r assessing the activity of these cyclases. Membrane preparations derived from this overexpression system provide homogeneous sources of mammalian AC because AC that are endogenously expressed in Sf9 cells contribute low amounts of activity relative to the overexpressed enzyme. These approaches are 作者: 大洪水 時(shí)間: 2025-3-28 14:40 作者: abject 時(shí)間: 2025-3-28 19:39
Sanja Runti?,Jana Mare?ová,Klára Kolinskácatalyzes the hydrolysis of phosphotidylinositol 4,5-bisphosphates (PIP.) to inositol trisphosphate (IP.) and diacylglycerol (DAG). This chapter describes a cell-based assay system to determine the activity of PLC by monitoring the levels of IPs in cultured cells. This assay system can be used to ex作者: 怎樣才咆哮 時(shí)間: 2025-3-29 00:08 作者: ablate 時(shí)間: 2025-3-29 04:04 作者: 千篇一律 時(shí)間: 2025-3-29 10:38
(Un)wirtschaftliche HaushaltsführungKnowledge of the subcellular distribution of GPCRs is required in many experimental situations. Most GPCR signaling occurs in response to activators that interact with receptors localized on the cell surface. GPCRs must move from their site of synthesis to the plasma membrane, often undergoing redis作者: Carcinoma 時(shí)間: 2025-3-29 13:42 作者: Veneer 時(shí)間: 2025-3-29 16:17
De fysiologie van de glucosehuishouding,ferase reporter gene assay system is described for the study of the coupling of GPCRs to Gα.. This assay system, in which ligand-stimulated production of the reporter luciferase under the control of a modified serum-responsive element (SRE) in a cell line derived from mice lacking Gα. that are trans作者: Accommodation 時(shí)間: 2025-3-29 20:06
https://doi.org/10.1007/978-3-322-97190-6members of G protein α subunits and, in most cases, act as GTPase activating proteins (GAPs). RGS proteins interact with activated forms of Gα subunits through RGS domain of about 120 amino acids. Thus, the overexpression of the RGS domain in cells can specifically block the signalling pathways that作者: dilute 時(shí)間: 2025-3-30 02:51 作者: Jubilation 時(shí)間: 2025-3-30 07:26
Purification of G Protein Subunits from Sf9 Insect Cells Using Hexahistidine-Tagged α and βγ Subunitcombinant G proteins are critically important. Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits.作者: 軍火 時(shí)間: 2025-3-30 11:26
